{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T02:32:15Z","timestamp":1788229935687,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Dental and Craniofacial Research (NIDCR)","award":["R01DE026433"],"award-info":[{"award-number":["R01DE026433"]}]},{"name":"National Institute of Dental and Craniofacial Research (NIDCR)","award":["R01DE029159"],"award-info":[{"award-number":["R01DE029159"]}]},{"name":"National Institute of Dental and Craniofacial Research (NIDCR)","award":["F31DE031153"],"award-info":[{"award-number":["F31DE031153"]}]},{"name":"National Institute of Dental and Craniofacial Research (NIDCR)","award":["T90DE023520"],"award-info":[{"award-number":["T90DE023520"]}]},{"name":"National Institutes of Health (NIH)","award":["R01DE026433"],"award-info":[{"award-number":["R01DE026433"]}]},{"name":"National Institutes of Health (NIH)","award":["R01DE029159"],"award-info":[{"award-number":["R01DE029159"]}]},{"name":"National Institutes of Health (NIH)","award":["F31DE031153"],"award-info":[{"award-number":["F31DE031153"]}]},{"name":"National Institutes of Health (NIH)","award":["T90DE023520"],"award-info":[{"award-number":["T90DE023520"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>MicroRNA (miR)-200c enhances osteogenesis, modulates inflammation, and participates in dentin development. This study was to investigate the beneficial potential of miR-200c in vital pulp therapy (VPT) by mitigating pulpitis and promoting dentin regeneration. We explored the miR-200c variations in inflamed pulp tissues from patients with symptomatic irreversible pulpitis and primary human dental pulp-derived cells (DPCs) challenged with P.g. lipopolysaccharide (Pg-LPS). We further assessed the functions of overexpression of miR-200c on odontogenic differentiation, pulpal inflammation, and dentin regeneration in vitro and in vivo. Our findings revealed a noteworthy downregulation of miR-200c expression in inflamed pulp tissues and primary human DPCs. Through the overexpression of miR-200c via transfecting plasmid DNA (pDNA), we observed a substantial downregulation of proinflammatory cytokines interleukin (IL)-6 and IL-8 in human DPCs. Furthermore, this overexpression significantly enhanced the transcript and protein levels of odontogenic differentiation markers, including Runt-related transcription factor (Runx)2, osteocalcin (OCN), dentin matrix protein (DMP)1, and dentin sialophosphoprotein (DSPP). In a rat model of pulpitis induced by Pg-LPS, we demonstrated notable benefits by local application of pDNA encoding miR-200c delivered by CaCO3-based nanoparticles to reduce pulpal inflammation and promote dentin formation. These results underscore the significant impact of locally applied miR-200c in modulating pulpal inflammation and facilitating dentin repair, showcasing its ability to improve VPT outcomes.<\/jats:p>","DOI":"10.3390\/ijms26146734","type":"journal-article","created":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T09:45:52Z","timestamp":1752572752000},"page":"6734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["microRNA-200c Mitigates Pulpitis and Promotes Dentin Regeneration"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1755-1381","authenticated-orcid":false,"given":"Tadkamol","family":"Krongbaramee","sequence":"first","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"},{"name":"Division of Endodontics, Department of Restorative Dentistry & Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8934-1618","authenticated-orcid":false,"given":"Chawin","family":"Upara","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Remy","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6388-7046","authenticated-orcid":false,"given":"Long","family":"Jiang","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jue","family":"Hu","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kittiphoj","family":"Tikkhanarak","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Radiology, and Medicine, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0455-4823","authenticated-orcid":false,"given":"Bruno","family":"Cavalcanti","sequence":"additional","affiliation":[{"name":"Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongli","family":"Sun","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2790-3499","authenticated-orcid":false,"given":"Fabricio B.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Endodontics, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0308-5049","authenticated-orcid":false,"given":"Liu","family":"Hong","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research, College of Dentistry, the University of Iowa, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"ref_1","first-page":"6","article-title":"A review on vital pulp therapy in primary teeth","volume":"10","author":"Parisay","year":"2015","journal-title":"Iran. 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