{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T13:37:12Z","timestamp":1785332232716,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"Consejer\u00eda de Salud de la Junta de Andaluc\u00eda","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"Fundaci\u00f3n Unicaja","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"UMA-FEDER &amp; ALIANZA MIXTA ANDALUC\u00cdA-ROCHE","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"Universidad de M\u00e1laga\/CBUA","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"Plan Propio de Investigaci\u00f3n, Transferencia y Divulgaci\u00f3n Cient\u00edfica de la Universidad de M\u00e1laga 2023","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"University Teacher Training of the Ministry of Science, Innovation, and Universities","doi-asserted-by":"publisher","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["PE-0106-2019"],"award-info":[{"award-number":["PE-0106-2019"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["C19047-2018"],"award-info":[{"award-number":["C19047-2018"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["UMA18-FEDERJA-042"],"award-info":[{"award-number":["UMA18-FEDERJA-042"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["FIS21\/00321"],"award-info":[{"award-number":["FIS21\/00321"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["FIS24\/00161"],"award-info":[{"award-number":["FIS24\/00161"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["CD23\/00110"],"award-info":[{"award-number":["CD23\/00110"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["FI19-00112"],"award-info":[{"award-number":["FI19-00112"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["CD21\/00164"],"award-info":[{"award-number":["CD21\/00164"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["FPU22\/01679"],"award-info":[{"award-number":["FPU22\/01679"]}]},{"name":"Personal T\u00e9cnico de Apoyo de la Agencia Estatal de Investigaci\u00f3n, Ministerio de Ciencias e Innovaci\u00f3n","award":["PTA2022-022049-I"],"award-info":[{"award-number":["PTA2022-022049-I"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Emerging evidence suggests a bidirectional relationship between gut microbiota, melatonin synthesis, and breast cancer (BC) development in hormone receptor-positive patients (HR+HER2+ and HR+HER2-). This study investigated alterations in gut microbiota composition, the serum serotonin\u2013N-acetylserotonin (NAS)\u2013melatonin axis, fecal short-chain fatty acids (SCFAs) and beta-glucuronidase (\u03b2GD) activity, and serum zonulin in HR+ BC patients compared to healthy controls. Blood and fecal samples were analyzed using mass spectrometry for serotonin, NAS, melatonin, and SCFAs; ELISA for AANAT, ASMT, 14-3-3 protein, and zonulin; fluorometric assay for \u03b2GD activity; and 16S rRNA sequencing for gut microbiota composition. HR+ BC patients exhibited gut dysbiosis with reduced Bifidobacterium longum and increased Bacteroides eggerthii, alongside elevated fecal \u03b2GD activity, SCFA levels (e.g., isovaleric acid), and serum zonulin, indicating increased intestinal permeability. Serum serotonin and N-acetylserotonin (NAS) levels were elevated, while melatonin levels were reduced, with a higher NAS\/melatonin ratio in BC patients. AANAT levels were increased, and ASMT levels were decreased, suggesting disrupted melatonin synthesis. Bifidobacterium longum positively correlated with melatonin and negatively with \u03b2GD activity, while Bacteroides eggerthii showed a positive correlation with \u03b2GD activity. These findings suggested that gut microbiota alterations, disrupted melatonin synthesis, microbial metabolism, and intestinal permeability may contribute to BC pathophysiology. The NAS\/melatonin ratio could represent a potential biomarker, necessitating further mechanistic studies to confirm causality and explore therapeutic interventions.<\/jats:p>","DOI":"10.3390\/ijms26146801","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T08:24:17Z","timestamp":1752654257000},"page":"6801","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Interplay Between Gut Microbiota and the Melatonergic Pathway in Hormone Receptor-Positive Breast Cancer"],"prefix":"10.3390","volume":"26","author":[{"given":"Aurora","family":"Laborda-Illanes","sequence":"first","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4776-285X","authenticated-orcid":false,"given":"Soukaina","family":"Boutriq","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2434-6562","authenticated-orcid":false,"given":"Luc\u00eda","family":"Aranega-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Castellano-Castillo","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"S\u00e1nchez-Alcoholado","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac","family":"Plaza-Andrades","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Peralta-Linero","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"Alba","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"},{"name":"Department of Medicine and Pediatrics, Faculty of Medicine, University of Malaga, 29071 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Carlos","family":"Fern\u00e1ndez-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Nutrition, Regional University Hospital of Malaga, Biomedical Research Institute of Malaga (IBIMA), Faculty of Medicine, University of Malaga, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8020-2531","authenticated-orcid":false,"given":"Alicia","family":"Gonz\u00e1lez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"},{"name":"Department of Endocrinology and Nutrition, Regional University Hospital of Malaga, Biomedical Research Institute of Malaga (IBIMA), Faculty of Medicine, University of Malaga, 29010 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Isabel","family":"Queipo-Ortu\u00f1o","sequence":"additional","affiliation":[{"name":"Medical Oncology Clinical Management Unit, Virgen de la Victoria University Hospital, M\u00e1laga Biomedical Research Institute (IBIMA) BIONAND Platform-CIMES-UMA, 29010 Malaga, Spain"},{"name":"Department of Surgical Specialties, Biochemical and Immunology, Faculty of Medicine, University of M\u00e1laga, 29071 Malaga, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Laborda-Illanes, A., Sanchez-Alcoholado, L., Dominguez-Recio, M.E., Jimenez-Rodriguez, B., Lavado, R., Comino-M\u00e9ndez, I., Alba, E., and Queipo-Ortu\u00f1o, M.I. (2020). Breast and Gut Microbiota Action Mechanisms in Breast Cancer Pathogenesis and Treatment. Cancers, 12.","DOI":"10.3390\/cancers12092465"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Parida, S., and Sharma, D. (2019). The Microbiome\u2013Estrogen Connection and Breast Cancer Risk. Cells, 8.","DOI":"10.3390\/cells8121642"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1615\/JEnvironPatholToxicolOncol.2022041294","article-title":"Melatonin: A Potential Antineoplastic Agent in Breast Cancer","volume":"41","author":"Samanta","year":"2022","journal-title":"J. Environ. Pathol. Toxicol. Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/aps.2010.225","article-title":"A Protective Effect of Melatonin on Intestinal Permeability Is Induced by Diclofenac via Regulation of Mitochondrial Function in Mice","volume":"32","author":"Mei","year":"2011","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Li, Y., Hao, Y., Fan, F., and Zhang, B. (2018). The Role of Microbiome in Insomnia, Circadian Disturbance and Depression. Front. Psychiatry, 9.","DOI":"10.3389\/fpsyt.2018.00669"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1096\/fj.14-259598","article-title":"Gut Microbes Promote Colonic Serotonin Production through an Effect of Short-Chain Fatty Acids on Enterochromaffin Cells","volume":"29","author":"Reigstad","year":"2015","journal-title":"FASEB J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0021-9258(18)64458-8","article-title":"Purification and Properties of Hydroxyindole-O-Methyl Transferase","volume":"236","author":"Axelrod","year":"1961","journal-title":"J. Biol. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1006\/frne.1999.0194","article-title":"Melatonin and Mammary Pathological Growth","volume":"21","author":"Cos","year":"2000","journal-title":"Front. Neuroendocrinol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3876","DOI":"10.1073\/pnas.0912531107","article-title":"N-Acetylserotonin Activates TrkB Receptor in a Circadian Rhythm","volume":"107","author":"Jang","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Laborda-Illanes, A., S\u00e1nchez-Alcoholado, L., Boutriq, S., Plaza-Andrades, I., Peralta-Linero, J., Alba, E., Gonz\u00e1lez-Gonz\u00e1lez, A., and Queipo-Ortu\u00f1o, M.I. (2021). A New Paradigm in the Relationship between Melatonin and Breast Cancer: Gut Microbiota Identified as a Potential Regulatory Agent. Cancers, 13.","DOI":"10.3390\/cancers13133141"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"101873","DOI":"10.1016\/j.esmoop.2023.101873","article-title":"63P The Impact of Disruption of Melatonin Secretion on the Structural-Functional Changes of the Microbiome and the Role of the Melatonin-Microbiome Axis in the Initiation of Carcinogenesis","volume":"8","author":"Tavartkiladze","year":"2023","journal-title":"ESMO Open"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Iesanu, M.I., Zahiu, C.D.M., Dogaru, I.-A., Chitimus, D.M., Pircalabioru, G.G., Voiculescu, S.E., Isac, S., Galos, F., Pavel, B., and O\u2019Mahony, S.M. (2022). Melatonin-Microbiome Two-Sided Interaction in Dysbiosis-Associated Conditions. Antioxidants, 11.","DOI":"10.3390\/antiox11112244"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e12598","DOI":"10.1111\/jpi.12598","article-title":"Melatonin Reduces Inflammatory Response in Human Intestinal Epithelial Cells Stimulated by Interleukin-1\u03b2","volume":"67","author":"Mannino","year":"2019","journal-title":"J. Pineal Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2646","DOI":"10.1089\/neu.2018.6012","article-title":"Melatonin Treatment Alleviates Spinal Cord Injury-Induced Gut Dysbiosis in Mice","volume":"36","author":"Jing","year":"2019","journal-title":"J. Neurotrauma"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"325","DOI":"10.5217\/ir.2019.00093","article-title":"Melatonin in the Colon Modulates Intestinal Microbiota in Response to Stress and Sleep Deprivation","volume":"18","author":"Park","year":"2020","journal-title":"Intest. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1007\/s11325-020-02246-3","article-title":"Relationships of Sleep Disturbance, Intestinal Microbiota, and Postoperative Pain in Breast Cancer Patients: A Prospective Observational Study","volume":"25","author":"Yao","year":"2020","journal-title":"Sleep Breath."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.bcp.2019.07.014","article-title":"Breast Cancer: Occluded Role of Mitochondria N-Acetylserotonin\/Melatonin Ratio in Co-Ordinating Pathophysiology","volume":"168","author":"Anderson","year":"2019","journal-title":"Biochem. Pharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Veres-Sz\u00e9kely, A., Sz\u00e1sz, C., Pap, D., Szebeni, B., Bokrossy, P., and Vannay, \u00c1. (2023). Zonulin as a Potential Therapeutic Target in Microbiota-Gut-Brain Axis Disorders: Encouraging Results and Emerging Questions. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24087548"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Dutta, D., and Lim, S.H. (2020). Bidirectional Interaction between Intestinal Microbiome and Cancer: Opportunities for Therapeutic Interventions. Biomark. Res., 8.","DOI":"10.1186\/s40364-020-00211-6"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Bonmati-Carrion, M.-A., and Tomas-Loba, A. (2021). Melatonin and Cancer: A Polyhedral Network Where the Source Matters. Antioxidants, 10.","DOI":"10.3390\/antiox10020210"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/s10096-023-04551-7","article-title":"Microbial Dynamics with CRC Progression: A Study of the Mucosal Microbiota at Multiple Sites in Cancers, Adenomatous Polyps, and Healthy Controls","volume":"42","author":"Senthakumaran","year":"2023","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1002\/cac2.12469","article-title":"Bifidobacterium Adolescentis Orchestrates CD143+ Cancer-Associated Fibroblasts to Suppress Colorectal Tumorigenesis by Wnt Signaling-Regulated GAS1","volume":"43","author":"Chen","year":"2023","journal-title":"Cancer Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"173596","DOI":"10.1016\/j.ejphar.2020.173596","article-title":"Anti-Tumor Effect of a Recombinant Bifidobacterium Strain Secreting a Claudin-Targeting Molecule in a Mouse Breast Cancer Model","volume":"887","author":"Shimizu","year":"2020","journal-title":"Eur. J. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Parisa, A., Roya, G., Mahdi, R., Shabnam, R., Maryam, E., and Malihe, T. (2020). Anti-Cancer Effects of Bifidobacterium Species in Colon Cancer Cells and a Mouse Model of Carcinogenesis. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0232930"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5296","DOI":"10.7150\/thno.55986","article-title":"Role of Serotonin Receptor Signaling in Cancer Cells and Anti-Tumor Immunity","volume":"11","author":"Karmakar","year":"2021","journal-title":"Theranostics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.2147\/IJGM.S310591","article-title":"Identification of Serotonin as a Predictive Marker for Breast Cancer Patients","volume":"14","author":"Xie","year":"2021","journal-title":"Int. J. Gen. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"R81","DOI":"10.1186\/bcr2448","article-title":"Altered Serotonin Physiology in Human Breast Cancers Favors Paradoxical Growth and Cell Survival","volume":"11","author":"Pai","year":"2009","journal-title":"Breast Cancer Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1021\/acsmedchemlett.7b00282","article-title":"Serotonin Analogues as Inhibitors of Breast Cancer Cell Growth","volume":"8","author":"Jose","year":"2017","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Balakrishna, P., George, S., Hatoum, H., and Mukherjee, S. (2021). Serotonin Pathway in Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22031268"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3540","DOI":"10.1073\/pnas.1119201109","article-title":"N-Acetyl Serotonin Derivatives as Potent Neuroprotectants for Retinas","volume":"109","author":"Shen","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Gehlert, S., Clanton, M., and on behalf of the Shift Work and Breast Cancer Strategic Advisory Group (2020). Shift Work and Breast Cancer. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17249544"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.critrevonc.2017.12.018","article-title":"Melatonin and Breast Cancer: Evidences from Preclinical and Human Studies","volume":"122","author":"Kubatka","year":"2018","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s10549-007-9617-5","article-title":"Melatonin, Environmental Light, and Breast Cancer","volume":"108","author":"Srinivasan","year":"2008","journal-title":"Breast Cancer Res. Treat."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/j.1600-079X.2008.00559.x","article-title":"Selective Estrogen Enzyme Modulator Actions of Melatonin in Human Breast Cancer Cells","volume":"45","author":"Gonzalez","year":"2008","journal-title":"J. Pineal Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.2147\/OTT.S298512","article-title":"Role and Therapeutic Potential of Melatonin in Various Type of Cancers","volume":"14","author":"Gurunathan","year":"2021","journal-title":"Onco Targets Ther."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1158\/1541-7786.MCR-16-0158","article-title":"Melatonin Represses Metastasis in Her2 -Postive Human Breast Cancer Cells by Suppressing RSK2 Expression","volume":"14","author":"Mao","year":"2016","journal-title":"Mol. Cancer Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2848","DOI":"10.1002\/ijc.33512","article-title":"A Novel Signature Predicts Recurrence Risk and Therapeutic Response in Breast Cancer Patients","volume":"148","author":"Tran","year":"2021","journal-title":"Int. J. Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"R53","DOI":"10.1186\/bcr3445","article-title":"Analysis of Polymorphisms in the Circadian-Related Genes and Breast Cancer Risk in Norwegian Nurses Working Night Shifts","volume":"15","author":"Zienolddiny","year":"2013","journal-title":"Breast Cancer Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Silva, Y.P., Bernardi, A., and Frozza, R.L. (2020). The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication. Front. Endocrinol., 11.","DOI":"10.3389\/fendo.2020.00025"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2268","DOI":"10.1016\/j.neuron.2022.04.024","article-title":"5-Hydroxytryptamine Produced by Enteric Serotonergic Neurons Initiates Colorectal Cancer Stem Cell Self-Renewal and Tumorigenesis","volume":"110","author":"Zhu","year":"2022","journal-title":"Neuron"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhao, Z.-X., Yuan, X., Cui, Y.-Y., Liu, J., Shen, J., Jin, B.-Y., Feng, B.-C., Zhai, Y.-J., Zheng, M.-Q., and Kou, G.-J. (2021). Melatonin Mitigates Oxazolone-Induced Colitis in Microbiota-Dependent Manner. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.783806"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1476-511X-8-21","article-title":"Lactic Acid Bacteria Affect Serum Cholesterol Levels, Harmful Fecal Enzyme Activity, and Fecal Water Content","volume":"8","author":"Lee","year":"2009","journal-title":"Lipids Health Dis."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Candeliere, F., Raimondi, S., Ranieri, R., Musmeci, E., Zambon, A., Amaretti, A., and Rossi, M. (2022). \u03b2-Glucuronidase Pattern Predicted From Gut Metagenomes Indicates Potentially Diversified Pharmacomicrobiomics. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.826994"},{"key":"ref_44","first-page":"242","article-title":"Evaluation of the Antiproliferative Effect of Bifidobacterium Longum BB-536 in Solid Tumor Cell Lines, Co-Cultured with Murine Splenocytes","volume":"45","author":"Tripodi","year":"2021","journal-title":"Biochim. Clin."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101815","DOI":"10.1016\/j.genrep.2023.101815","article-title":"Anti-Tumor Effects of Bacteroides fragilis and Bifidobacterium bifidum Culture Supernatants on Mouse Breast Cancer","volume":"33","author":"Karami","year":"2023","journal-title":"Gene Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"114283","DOI":"10.1016\/j.intimp.2025.114283","article-title":"Bifidobacterium Longum Subsp. Longum XZ01 Delays the Progression of Colon Cancer in Mice through the Interaction between the Microbial Spatial Distribution and Tumour Immunity","volume":"150","author":"Chen","year":"2025","journal-title":"Int. Immunopharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Dziarski, R., Park, S.Y., Kashyap, D.R., Dowd, S.E., and Gupta, D. (2016). Pglyrp-Regulated Gut Microflora Prevotella Falsenii, Parabacteroides Distasonis and Bacteroides Eggerthii Enhance and Alistipes Finegoldii Attenuates Colitis in Mice. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146162"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"11","DOI":"10.5217\/ir.2015.13.1.11","article-title":"Intestinal Permeability Regulation by Tight Junction: Implication on Inflammatory Bowel Diseases","volume":"13","author":"Lee","year":"2015","journal-title":"Intest. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1515\/bmc-2019-0017","article-title":"Endometriosis Pathoetiology and Pathophysiology: Roles of Vitamin A, Estrogen, Immunity, Adipocytes, Gut Microbiome and Melatonergic Pathway on Mitochondria Regulation","volume":"10","author":"Anderson","year":"2019","journal-title":"Biomol. Concepts"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Anderson, G., Carbone, A., and Mazzoccoli, G. (2021). Tryptophan Metabolites and Aryl Hydrocarbon Receptor in Severe Acute Respiratory Syndrome, Coronavirus-2 (SARS-CoV-2) Pathophysiology. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22041597"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1178646919855942","DOI":"10.1177\/1178646919855942","article-title":"Glioblastoma: Role of Mitochondria N-Acetylserotonin\/Melatonin Ratio in Mediating Effects of miR-451 and Aryl Hydrocarbon Receptor and in Coordinating Wider Biochemical Changes","volume":"12","author":"Anderson","year":"2019","journal-title":"Int. J. Tryptophan Res."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Benabou, M., Rolland, T., Leblond, C., Millot, G., Huguet, G., Delorme, R., Leboyer, M., Pagan, C., Callebert, J., and Maronde, E. (2017). Heritability of the Melatonin Synthesis Variability in Autism Spectrum Disorders. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-18016-3"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e479","DOI":"10.1038\/tp.2014.120","article-title":"The Serotonin-N-Acetylserotonin\u2013Melatonin Pathway as a Biomarker for Autism Spectrum Disorders","volume":"4","author":"Pagan","year":"2014","journal-title":"Transl. Psychiatry"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Takada, A., Shimizu, F., and Masuda, J. (2018). Measurement of Plasma Tryptophan Metabolites: Clinical and Experimental Application for Depression and Stress States Assessment, IntechOpen.","DOI":"10.5772\/intechopen.78560"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1124\/pr.55.2.2","article-title":"Generation of the Melatonin Endocrine Message in Mammals: A Review of the Complex Regulation of Melatonin Synthesis by Norepinephrine, Peptides, and Other Pineal Transmitters","volume":"55","author":"Simonneaux","year":"2003","journal-title":"Pharmacol. Rev."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-Resolution Sample Inference from Illumina Amplicon Data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Calgaro, M., Romualdi, C., Waldron, L., Risso, D., and Vitulo, N. (2020). Assessment of Statistical Methods from Single Cell, Bulk RNA-Seq, and Metagenomics Applied to Microbiome Data. Genome Biol., 21.","DOI":"10.1186\/s13059-020-02104-1"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e12626","DOI":"10.1111\/jpi.12626","article-title":"Characterization of Serotonin and N-Acetylserotonin Systems in the Human Epidermis and Skin Cells","volume":"68","author":"Slominski","year":"2020","journal-title":"J. Pineal Res."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Kaliannan, K., Donnell, S.O., Murphy, K., Stanton, C., Kang, C., Wang, B., Li, X.-Y., Bhan, A.K., and Kang, J.X. (2022). Decreased Tissue Omega-6\/Omega-3 Fatty Acid Ratio Prevents Chemotherapy-Induced Gastrointestinal Toxicity Associated with Alterations of Gut Microbiome. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23105332"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2108279","DOI":"10.1080\/19490976.2022.2108279","article-title":"Fecal \u03b2-Glucuronidase Activity Differs between Hematopoietic Cell and Kidney Transplantation and a Possible Mechanism for Disparate Dose Requirements","volume":"14","author":"Khan","year":"2022","journal-title":"Gut Microbes"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"5555","DOI":"10.1007\/s00216-017-0493-5","article-title":"Development of a Fast and Cost-Effective Gas Chromatography\u2013Mass Spectrometry Method for the Quantification of Short-Chain and Medium-Chain Fatty Acids in Human Biofluids","volume":"409","author":"Lotti","year":"2017","journal-title":"Anal. Bioanal. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/14\/6801\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:10:24Z","timestamp":1760033424000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/14\/6801"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,16]]},"references-count":62,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["ijms26146801"],"URL":"https:\/\/doi.org\/10.3390\/ijms26146801","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,16]]}}}