{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T06:33:34Z","timestamp":1780727614567,"version":"3.54.1"},"reference-count":113,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T00:00:00Z","timestamp":1752624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Environmental Health Sciences","award":["R01 ES030722"],"award-info":[{"award-number":["R01 ES030722"]}]},{"name":"National Institute of Environmental Health Sciences","award":["F31 ES030278"],"award-info":[{"award-number":["F31 ES030278"]}]},{"name":"National Institute of Environmental Health Sciences","award":["K01 OD01462"],"award-info":[{"award-number":["K01 OD01462"]}]},{"name":"National Center for Advancement of Translational Sciences","award":["R01 ES030722"],"award-info":[{"award-number":["R01 ES030722"]}]},{"name":"National Center for Advancement of Translational Sciences","award":["F31 ES030278"],"award-info":[{"award-number":["F31 ES030278"]}]},{"name":"National Center for Advancement of Translational Sciences","award":["K01 OD01462"],"award-info":[{"award-number":["K01 OD01462"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) exposure has long been associated with reproductive dysfunction in males and females even at miniscule levels, which can persist across generations. Given the continued industrial use and detection of other aryl hydrocarbon receptor (AhR) agonists in the general population and the demonstrated heritable phenotypes of TCDD exposure, further work is justified to elucidate reproductive pathologies and minimize exposure risk. In females, multi- and transgenerational subfertility has been demonstrated in a zebrafish (Danio rerio) model exposed to 50 pg\/mL TCDD once at 3 and 7 weeks post fertilization (wpf). We further characterize the histopathologic, hormonal and transcriptomic outcomes of the mature female zebrafish ovary following early-life TCDD exposure. Exposure was associated with significantly increased ovarian atresia in the F0 and F1, but not F2 generation. Other oocyte staging and vitellogenesis were unaffected in all generations. Exposed F0 females showed increased levels of whole-body triiodothyronine (T3) and 17\u03b2-estradiol (E2) levels, but not vitellogenin (Vtg), 11-ketotestosterone (11-KT), cortisol, thyroxine (T4), or testosterone (T). Ovarian transcriptomics were most dysregulated in the F2. Both F0 and F2, but not F1, showed changes in epigenetic-related gene expression. Rho signaling was the top pathway for both F0 and F2.<\/jats:p>","DOI":"10.3390\/ijms26146839","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T15:48:22Z","timestamp":1752680902000},"page":"6839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Multi- and Transgenerational Histological and Transcriptomic Outcomes of Developmental TCDD Exposure in Zebrafish (Danio rerio) Ovary"],"prefix":"10.3390","volume":"26","author":[{"given":"Amelia","family":"Paquette","sequence":"first","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Cavaneau","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Haimbaugh","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle N.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Akemann","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Wayne State University, Detroit, MI 48202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Dennis","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5057-4155","authenticated-orcid":false,"given":"Tracie R.","family":"Baker","sequence":"additional","affiliation":[{"name":"Department of Environmental and Global Health, University of Florida, Gainesville, FL 32611, USA"},{"name":"Department of Pharmacology, Wayne State University, Detroit, MI 48202, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1375\/twin.4.5.293","article-title":"Effects of prenatal exposure to the Dutch famine on adult disease in later life: An overview","volume":"4","author":"Roseboom","year":"2001","journal-title":"Twin Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4097","DOI":"10.1210\/en.2012-1422","article-title":"Endocrine-disrupting chemicals and public health protection: A statement of principles from The Endocrine Society","volume":"153","author":"Zoeller","year":"2012","journal-title":"Endocrinology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Manikkam, M., Tracey, R., Guerrero-Bosagna, C., and Skinner, M.K. 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