{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:46:30Z","timestamp":1789494390302,"version":"build-2803163510"},"reference-count":63,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["2024TR002502"],"award-info":[{"award-number":["2024TR002502"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["2023TR001472"],"award-info":[{"award-number":["2023TR001472"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2024TR002502"],"award-info":[{"award-number":["2024TR002502"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2023TR001472"],"award-info":[{"award-number":["2023TR001472"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Estado de Santa Catarina","award":["2024TR002502"],"award-info":[{"award-number":["2024TR002502"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa e Inova\u00e7\u00e3o do Estado de Santa Catarina","award":["2023TR001472"],"award-info":[{"award-number":["2023TR001472"]}]},{"name":"Federal University of Fronteira Sul (UFFS)","award":["2024TR002502"],"award-info":[{"award-number":["2024TR002502"]}]},{"name":"Federal University of Fronteira Sul (UFFS)","award":["2023TR001472"],"award-info":[{"award-number":["2023TR001472"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pituitary neuroendocrine tumors (PitNETs) are the most common intracranial tumors. Evidence suggests that these types of tumors may have high recurrence rates. In this context, the purinergic system, oxidative stress, and inflammation are important signaling pathways involved in the cancer\u2019s pathophysiology. This study aimed to evaluate the sociodemographic and diagnostic profiles, as well as assess the purinergic signaling, immunological, and redox profiles, of patients after PitNET resection. We collected sociodemographic data and the patients\u2019 diagnostic profiles. We also collected blood samples to analyze glycemia, triglycerides, albumin, and ATP levels. The ectonucleotidase activity was determined in peripheral blood mononuclear cells (PBMCs). In addition, we evaluated their redox and immunological profiles. There was a prevalence of gonadotropic macroadenoma derived from PIT-1 cells. We found that patients included in the PitNET group had increased glycemia, serum ATP levels, and ATP hydrolysis in PBMCs. Analyzing their immunological profiles, we found that patients had increased levels of IL-6, IL-10, and TNF, while the IL-27 level was decreased. Regarding their redox profiles, PitNET patients had increased levels of ROS and protein carbonylation. Unexpectedly, patients also showed increased levels of non-protein thiols (NPSHs), total thiols (PSHs), and ascorbic acid. Thus, the dysregulation of purinergic signaling sustained chronic inflammation and oxidative imbalance in PitNET patients for a long time after surgical resection. These data suggest that patients with PitNETs require long-term accompanying to prevent cancer recurrence prognosis. The biomarkers highlighted in this study may be good tools to help the medical approaches.<\/jats:p>","DOI":"10.3390\/ijms26146890","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T15:15:54Z","timestamp":1752765354000},"page":"6890","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dysregulation of Purinergic Signaling Sustains Chronic Inflammation and Oxidative Imbalance in Patients After PitNET Surgical Resection"],"prefix":"10.3390","volume":"26","author":[{"given":"Geile","family":"Fistarol","sequence":"first","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz A.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilnei B.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Multicentric Postgraduate Program in Biochemistry and Molecular Biology, State University of Santa Catarina, Lages 88520-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane","family":"Manica","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biochemistry, University of Santa Catarina, Florian\u00f3polis 88040-970, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marceli C.","family":"Hanauer","sequence":"additional","affiliation":[{"name":"Department of Nursing, Postgraduate Program in Nursing, Federal University of Santa Catarina, Florian\u00f3polis 88040-970, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Dallagnol","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael A.","family":"Narzetti","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biochemistry, University of Santa Catarina, Florian\u00f3polis 88040-970, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8604-471X","authenticated-orcid":false,"given":"Maria L.","family":"Bergamini","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4286-5083","authenticated-orcid":false,"given":"Vit\u00f3ria C.","family":"de Melo","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tais","family":"Vidal","sequence":"additional","affiliation":[{"name":"Center of Health Sciences, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6753-6083","authenticated-orcid":false,"given":"Micheli M.","family":"Pillat","sequence":"additional","affiliation":[{"name":"Center of Health Sciences, Federal University of Santa Maria, Santa Maria 97105-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jussara","family":"de Lima","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"},{"name":"Regional Hospital of West, Center of Neurosurgery, Chapec\u00f3 89812-505, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo L. V.","family":"da Cunha","sequence":"additional","affiliation":[{"name":"Regional Hospital of West, Center of Neurosurgery, Chapec\u00f3 89812-505, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marielle L.","family":"Makiyama","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Marafon","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7864-7416","authenticated-orcid":false,"given":"Aniela P.","family":"Kempka","sequence":"additional","affiliation":[{"name":"Multicentric Postgraduate Program in Biochemistry and Molecular Biology, State University of Santa Catarina, Lages 88520-000, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2844-8563","authenticated-orcid":false,"given":"Ariane","family":"Zamoner","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biochemistry, University of Santa Catarina, Florian\u00f3polis 88040-970, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9263-4980","authenticated-orcid":false,"given":"Margarete D.","family":"Bagatini","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biomedical Science, Federal University of Fronteira Sul, Chapec\u00f3 89815-899, SC, Brazil"},{"name":"Postgraduate Program in Biochemistry, University of Santa Catarina, Florian\u00f3polis 88040-970, SC, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1038\/s41379-021-00820-y","article-title":"Pituitary Neuroendocrine Tumors: A Model for Neuroendocrine Tumor Classification","volume":"34","author":"Asa","year":"2021","journal-title":"Mod. 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