{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T20:15:47Z","timestamp":1768680947658,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Program of the Sirius Federal Territory \u201cScientific and technological development of the Sirius Federal Territory\u201d","award":["18-03"],"award-info":[{"award-number":["18-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Protein association and aggregation are fundamental processes that play critical roles in a variety of biological phenomena from cell signaling to the development of incurable diseases, including amyloidoses. Understanding the basic biophysical principles governing protein aggregation processes is of crucial importance for developing treatment strategies for diseases associated with protein aggregation, including sarcopenia, as well as for the treatment of pathological processes associated with the disruption of functional protein complexes. This work, using a set of methods such as atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction, as well as bioinformatics analysis, investigated the structures of complexes formed by titin and myosin-binding protein C (MyBP-C). TEM revealed the formation of morphologically ordered aggregates in the form of beads during co-incubation of titin and MyBP-C under close-to-physiological conditions (175 mM KCl, pH 7.0). AFM showed the formation of a relatively homogeneous film with local areas of relief change. Fluorimetry with thioflavin T, as well as FTIR spectroscopy, revealed signs of an amyloid-like structure, including a signal in the cross-\u03b2 region. X-ray diffraction showed the presence of a cross-\u03b2 structure characteristic of amyloid aggregates. Such structural features were not observed in the control samples of the investigated proteins separately. In sarcomeres, these proteins are associated with each other, and this interaction plays a partial role in the formation of a strong sarcomeric cytoskeleton. We found that under physiological ionic-strength conditions titin and MyBP-C form complexes in which an amyloid-like structure is present. The possible functional significance of amyloid-like aggregation of these proteins in muscle cells in vivo is discussed.<\/jats:p>","DOI":"10.3390\/ijms26146910","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T11:12:34Z","timestamp":1752837154000},"page":"6910","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Formation of an Amyloid-like Structure During In Vitro Interaction of Titin and Myosin-Binding Protein C"],"prefix":"10.3390","volume":"26","author":[{"given":"Tatiana A.","family":"Uryupina","sequence":"first","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liya G.","family":"Bobyleva","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2212-5640","authenticated-orcid":false,"given":"Nikita V.","family":"Penkov","sequence":"additional","affiliation":[{"name":"Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2547-8804","authenticated-orcid":false,"given":"Maria A.","family":"Timchenko","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"},{"name":"Institute for Biological Instrumentation, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1016-5936","authenticated-orcid":false,"given":"Azat G.","family":"Gabdulkhakov","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences,142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna V.","family":"Glyakina","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences,142290 Pushchino, Russia"},{"name":"Institute of Mathematical Problems of Biology, Russian Academy of Sciences, Branch of the Keldysh Institute of Applied Mathematics, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9611-419X","authenticated-orcid":false,"given":"Vadim V.","family":"Rogachevsky","sequence":"additional","affiliation":[{"name":"Institute of Cell Biophysics, Federal Research Center Pushchino Scientific Center for Biological Research, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9246-7369","authenticated-orcid":false,"given":"Alexey K.","family":"Surin","sequence":"additional","affiliation":[{"name":"Institute of Protein Research, Russian Academy of Sciences,142290 Pushchino, Russia"},{"name":"Branch of the Shemyakin\u2013Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 142290 Pushchino, Russia"},{"name":"State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3962-1520","authenticated-orcid":false,"given":"Oxana V.","family":"Galzitskaya","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"},{"name":"Institute of Protein Research, Russian Academy of Sciences,142290 Pushchino, Russia"},{"name":"Gamaleya Research Centre of Epidemiology and Microbiology, 123098 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6063-6789","authenticated-orcid":false,"given":"Ivan M.","family":"Vikhlyantsev","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9477-1214","authenticated-orcid":false,"given":"Alexander G.","family":"Bobylev","sequence":"additional","affiliation":[{"name":"Scientific Center of Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sirius Federal Territory, 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