{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:36:25Z","timestamp":1787160985684,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korean government (MOTIE)","award":["RS-2024-00409639"],"award-info":[{"award-number":["RS-2024-00409639"]}]},{"name":"Korean government (MOTIE)","award":["RS-2023-00281517"],"award-info":[{"award-number":["RS-2023-00281517"]}]},{"name":"Korean government (MSIT)","award":["RS-2024-00409639"],"award-info":[{"award-number":["RS-2024-00409639"]}]},{"name":"Korean government (MSIT)","award":["RS-2023-00281517"],"award-info":[{"award-number":["RS-2023-00281517"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study investigated the natural bioactive compounds in Polygonum cuspidatum Sieb. et Zucc. (P. cuspidatum) by fractionating a 70% ethanol extract using n-hexane, chloroform, ethyl acetate, n-butanol, and water. The total polyphenol and flavonoid contents of each fraction were determined, and their antioxidant activities were evaluated using DPPH, ABTS, and FRAP assays. Additionally, the anti-diabetic potential was assessed via \u03b1-glucosidase inhibitory activity, while anti-obesity activity was evaluated using lipase inhibitory activity. The fractions were also tested for tyrosinase and elastase inhibitory activities to assess their skin-whitening and anti-wrinkle potential, and their antibacterial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was determined using the agar diffusion method. Finally, bioactive compounds were identified and quantified using HPLC and GC\u2013MSD. The results showed that the ethyl acetate fraction possessed the highest total polyphenol content (0.53 \u00b1 0.01 g GAE\/g) and total flavonoid content (0.19 \u00b1 0.02 g QE\/g). It also exhibited strong antioxidant activity, with the lowest DPPH radical scavenging IC50 (0.01 \u00b1 0.00 mg\/mL), ABTS radical scavenging IC50 (0.06 \u00b1 0.00 mg\/mL), and the highest FRAP value (6.02 \u00b1 0.30 mM Fe2+\/mg). Moreover, it demonstrated potent enzyme inhibitory activities, including tyrosinase inhibitory activity (67.78 \u00b1 2.50%), elastase inhibitory activity (83.84 \u00b1 1.64%), \u03b1-glucosidase inhibitory activity (65.14 \u00b1 10.29%), and lipase inhibitory activity (85.79 \u00b1 1.04%). In the antibacterial activity, the ethyl acetate fraction produced a clear inhibitory zone of 19.50 mm against Staphylococcus aureus, indicating notable antibacterial activity. HPLC-PDA and GC\u2013MSD analyses identified tannic acid and emodin as the major bioactive constituents. These findings suggest that the ethyl acetate fraction of P. cuspidatum extract, rich in polyphenol and flavonoid compounds, is a promising natural source of bioactive ingredients for applications in the food, pharmaceutical, and cosmetic industries. Further research is needed to explore its mechanisms and therapeutic applications.<\/jats:p>","DOI":"10.3390\/ijms26147011","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T13:59:11Z","timestamp":1753106351000},"page":"7011","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Solvent Fractionation of Polygonum cuspidatum Sieb. et Zucc. for Antioxidant, Biological Activity, and Chromatographic Characterization"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2190-1433","authenticated-orcid":false,"given":"Yuchen","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Chemistry, Hannam University, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Hannam University, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1787-8939","authenticated-orcid":false,"given":"Woonjung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Hannam University, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4067\/S0716-97602007000100002","article-title":"Antioxidant activity of extract from Polygonum","volume":"40","author":"Hsu","year":"2007","journal-title":"Biol. 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