{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:57:06Z","timestamp":1792069026635,"version":"4.4.0"},"reference-count":43,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["BR21882184"],"award-info":[{"award-number":["BR21882184"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Roux-en-Y gastric bypass (RYGB) surgery induces profound gut microbiota alterations that may impact metabolic outcomes. This study investigated site-specific effects of a multicomponent bovine colostrum-honey-serviceberry (CHJ) complex on post-RYGB microbiome changes in obese rats. Twenty-nine Wistar rats underwent RYGB surgery with CHJ supplementation, followed by mucosal-associated microbiota analysis from five gastrointestinal segments using 16S rRNA sequencing and serum metabolite profiling. RYGB caused regional-specific changes: decreased alpha diversity, systematic Proteobacteria increases (31.2 \u00b1 5.1% in duodenum), and reductions in SCFA-producing bacteria (Romboutsia, Roseburia). CHJ supplementation exhibited dual effects on the microbiome: restoration of beneficial bacteria (Lactobacillus, Bifidobacterium) in distal segments while concurrently promoting Enterobacteriaceae growth in proximal regions. CHJ also maintained alpha diversity levels of the mucosa-associated microbiota comparable to those observed in the control group. Disconnects emerged between predicted microbial functions and systemic metabolites: thiamine pathway activation accompanied 78.5% serum vitamin B1 reduction, indicating severe absorption deficits. Three distinct patterns emerged: pro-inflammatory (proximal), decolonization (widespread Helicobacteraceae loss), and restorative (selective CHJ-mediated recovery). Results demonstrate that post-RYGB dysbiosis exhibits profound regional heterogeneity requiring segment-specific interventions and highlight complex interactions between nutritional supplementation and surgically altered gut ecology in determining metabolic outcomes.<\/jats:p>","DOI":"10.3390\/ijms26157186","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T11:38:41Z","timestamp":1753443521000},"page":"7186","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Site-Specific Gut Microbiome Changes After Roux-en-Y Gastric Bypass in Rats: Effects of a Multicomponent Bovine Colostrum-Based Complex"],"prefix":"10.3390","volume":"26","author":[{"given":"Zhanagul","family":"Khassenbekova","sequence":"first","affiliation":[{"name":"Innovative Center ArtScience, Astana Z00T3X6, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4128-6482","authenticated-orcid":false,"given":"Kadyrzhan","family":"Makangali","sequence":"additional","affiliation":[{"name":"Department of Technology of Food and Processing Industry, S. Seifullin Kazakh Agrotechnical Research University, Astana Z11F9K5, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruzhan","family":"Shoman","sequence":"additional","affiliation":[{"name":"Department of Technology of Food and Processing Industry, S. Seifullin Kazakh Agrotechnical Research University, Astana Z11F9K5, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5480-933X","authenticated-orcid":false,"given":"Assem","family":"Sagandyk","sequence":"additional","affiliation":[{"name":"Department of Technology of Food and Processing Industry, S. Seifullin Kazakh Agrotechnical Research University, Astana Z11F9K5, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurislam","family":"Mukhanbetzhanov","sequence":"additional","affiliation":[{"name":"Innovative Center ArtScience, Astana Z00T3X6, Kazakhstan"},{"name":"Laboratory of Microbiome, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Ave., Block S1, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6246-4431","authenticated-orcid":false,"given":"Farkhad","family":"Tarikhov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiome, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Ave., Block S1, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timur","family":"Fazylov","sequence":"additional","affiliation":[{"name":"Innovative Center ArtScience, Astana Z00T3X6, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2037-1100","authenticated-orcid":false,"given":"Ylham","family":"Annaorazov","sequence":"additional","affiliation":[{"name":"Innovative Center ArtScience, Astana Z00T3X6, Kazakhstan"},{"name":"Department of Surgery and Anesthesiology-Intensive Care, Khoja Akhmet Yassawi International Kazakh-Turkish University, Shymkent X83B2G1, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1845-2726","authenticated-orcid":false,"given":"Elizaveta","family":"Vinogradova","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiome, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Ave., Block S1, Astana Z05H0P9, Kazakhstan"},{"name":"Interdisciplinary Sports Research, Center for Genetics and Life Sciences, Sirius University of Science and Technology, 1 Olympic Ave., Sirius Federal Territory, Sochi 354340, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9668-0327","authenticated-orcid":false,"given":"Samat","family":"Kozhakhmetov","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiome, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Ave., Block S1, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9479-0899","authenticated-orcid":false,"given":"Almagul","family":"Kushugulova","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiome, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Ave., Block S1, Astana Z05H0P9, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109924","DOI":"10.1016\/j.diabres.2022.109924","article-title":"Geographic distribution of metabolic syndrome and its components in the general adult population: A meta-analysis of global data from 28 million individuals","volume":"188","author":"Noubiap","year":"2022","journal-title":"Diabetes Res. 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