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The root culture of this species might represent a sustainable source of several pharmacologically active compounds. The biomass of a root T. parthenium culture was extracted with methanol and fractionated using column chromatography. Three selected fractions (4TP, 5TP, and 8TP) were analyzed via spectrophotometric, chromatographic, and mass spectrometry techniques and in vitro pharmacological assays. The greatest values for total phenolic and phenolic acid contents and antibacterial activity against Escherichia coli were determined for 4TP. The highest values for total flavonoid and sesquiterpene lactone contents, antioxidant potential, and \u03b1-amylase inhibitory effect were determined for 8TP. The antibacterial effect against Staphylococcus aureus was not significantly different among the three fractions. The root culture of T. parthenium is a potential source of several metabolites, such as phenolic acids, fatty acids, coumarins, sesquiterpenoids, and triterpenoids, which are capable of exerting \u03b1-amylase inhibition and antioxidant, antibacterial, and cytotoxic effects. Among eight phenolic compounds detected and quantified in the fractions, chlorogenic acid was the most abundant.<\/jats:p>","DOI":"10.3390\/ijms26157209","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T12:56:43Z","timestamp":1753448203000},"page":"7209","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Metabolomic and Pharmacological Approaches for Exploring the Potential of Tanacetum parthenium L. Root Culture as a Source of Bioactive Phytochemicals"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9921-732X","authenticated-orcid":false,"given":"Aurelio","family":"Nieto-Trujillo","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Recursos Bi\u00f3ticos, Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Carretera Toluca-Ixtlahuaca km 14.5, San Cayetano, Toluca 50295, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5822-6956","authenticated-orcid":false,"given":"Rosendo","family":"Luria-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Enfermedades Oncol\u00f3gicas, Hospital Infantil de M\u00e9xico Federico G\u00f3mez, Dr. M\u00e1rquez No 162, Col. Doctores, Cuauht\u00e9moc, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-7264","authenticated-orcid":false,"given":"Francisco","family":"Cruz-Sosa","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Universidad Aut\u00f3noma Metropolitana-Unidad Iztapalapa, Av. Ferrocarril San Rafael Atlixco No 186, Leyes de Reforma 1ra Secci\u00f3n, Iztapalapa, Mexico City 09310, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2555-0557","authenticated-orcid":false,"given":"Carmen","family":"Zepeda-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Campus El Cerrillo, Piedras Blancas, Carretera Toluca-Ixtlahuaca km. 15.5, Toluca 50200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6095-9673","authenticated-orcid":false,"given":"Mar\u00eda G.","family":"Gonz\u00e1lez-Pedroza","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Campus El Cerrillo, Piedras Blancas, Carretera Toluca-Ixtlahuaca km. 15.5, Toluca 50200, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3499-8668","authenticated-orcid":false,"given":"Cristina","family":"Burrola-Aguilar","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Recursos Bi\u00f3ticos, Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Carretera Toluca-Ixtlahuaca km 14.5, San Cayetano, Toluca 50295, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4685-5322","authenticated-orcid":false,"given":"Armando","family":"Sunny","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Ciencias Biol\u00f3gicas Aplicadas, Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Carretera Toluca-Ixtlahuaca km 14.5, San Cayetano, Toluca 50295, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4973-5265","authenticated-orcid":false,"given":"Jos\u00e9","family":"Correa-Basurto","sequence":"additional","affiliation":[{"name":"Laboratorio de Modelado Molecular, Bioinform\u00e1tica y Dise\u00f1o de F\u00e1rmacos, Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y Salvador D\u00edaz Mir\u00f3n S\/N, Casco de Santo Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0998-757X","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Guerrero-Analco","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecolog\u00eda, A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2394-0524","authenticated-orcid":false,"given":"Juan L.","family":"Monribot-Villanueva","sequence":"additional","affiliation":[{"name":"Laboratorio de Qu\u00edmica de Productos Naturales, Red de Estudios Moleculares Avanzados, Instituto de Ecolog\u00eda, A.C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5966-1423","authenticated-orcid":false,"given":"Mar\u00eda Elena","family":"Estrada-Z\u00fa\u00f1iga","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Recursos Bi\u00f3ticos, Facultad de Ciencias, Universidad Aut\u00f3noma del Estado de M\u00e9xico, Carretera Toluca-Ixtlahuaca km 14.5, San Cayetano, Toluca 50295, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Twaij, B.M., Jazar, Z.H., and Hasan, M.N. 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