{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T11:03:57Z","timestamp":1785927837581,"version":"3.56.0"},"reference-count":34,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007686","name":"Instituto Nacional de Perinatolog\u00eda","doi-asserted-by":"publisher","award":["2019-1-26"],"award-info":[{"award-number":["2019-1-26"]}],"id":[{"id":"10.13039\/501100007686","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico for Dora Mar\u00eda Vel\u00e1zquez-Hern\u00e1ndez","award":["2019-1-26"],"award-info":[{"award-number":["2019-1-26"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometriosis is a disorder characterized by the presence of endometrial tissue outside the uterus, leading to dyspareunia, chronic pelvic pain, dysuria, and infertility. The latter has been related to implantation failure associated with alterations in decidualization, a process regulated by sex hormones such as progesterone. Membrane progesterone receptor \u03b2 (mPR\u03b2) exhibits a lower expression in endometriotic tissues than in normal endometrial ones. However, the role of mPR\u03b2 in decidualization is unknown. This work aimed to investigate whether mPR\u03b2 plays a role in the decidualization of endometrial stromal cells (ESCs) derived from women with and without endometriosis. The mPR agonist OrgOD-2 induced the gene expression of key decidualization markers (insulin-like growth factor binding protein 1, prolactin, transcription factor heart and neural crest derivatives-expressed transcript 2, and fork-head transcription factor) in healthy ESCs, eutopic (uterine cavity), and ectopic (outside of the uterine cavity) ESCs from women with endometriosis. Notably, the expression of the decidualization markers was lower in endometriotic cells than in healthy endometrial ones. An siRNA mediated knockdown of mPR\u03b2 reduced the expression of decidualization-associated genes in ESCs treated with a decidualization stimuli, regardless of whether cells were derived from healthy women or those with endometriosis. Our data suggest that progesterone, through mPR\u03b2 activation, regulates the decidualization process in endometrial stromal cells from women with and without endometriosis.<\/jats:p>","DOI":"10.3390\/ijms26157297","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T16:00:37Z","timestamp":1753718437000},"page":"7297","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Membrane Progesterone Receptor Beta Regulates the Decidualization of Endometrial Stromal Cells in Women with Endometriosis"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9343-9036","authenticated-orcid":false,"given":"Dora Maria","family":"Vel\u00e1zquez-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Reproducci\u00f3n Humana, Instituto Nacional de Perinatolog\u00eda-Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7151-2976","authenticated-orcid":false,"given":"Edgar Ricardo","family":"V\u00e1zquez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Reproducci\u00f3n Humana, Instituto Nacional de Perinatolog\u00eda-Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9972-1214","authenticated-orcid":false,"given":"Oliver","family":"Cruz-Orozco","sequence":"additional","affiliation":[{"name":"Departamento de Ginecolog\u00eda Quir\u00fargica, Instituto Nacional de Perinatolog\u00eda, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9548-3280","authenticated-orcid":false,"given":"Jos\u00e9 Roberto","family":"Silvestri-Tomassoni","sequence":"additional","affiliation":[{"name":"Departamento de Ginecolog\u00eda Quir\u00fargica, Instituto Nacional de Perinatolog\u00eda, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"S\u00e1nchez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Ginecolog\u00eda Quir\u00fargica, Instituto Nacional de Perinatolog\u00eda, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4740-7994","authenticated-orcid":false,"given":"Andrea","family":"Olgu\u00edn-Ortega","sequence":"additional","affiliation":[{"name":"Departamento de Ginecolog\u00eda Quir\u00fargica, Instituto Nacional de Perinatolog\u00eda, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6384-6823","authenticated-orcid":false,"given":"Luis F.","family":"Escobar-Ponce","sequence":"additional","affiliation":[{"name":"Coordinaci\u00f3n de Cirug\u00eda P\u00e9vica Avanzada, Instituto Nacional de Perinatolog\u00eda, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4249-7222","authenticated-orcid":false,"given":"Mauricio","family":"Rodr\u00edguez-Dorantes","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Medicina Gen\u00f3mica, Perif\u00e9rico Sur 4809, Ciudad de M\u00e9xico 14610, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2978-1253","authenticated-orcid":false,"given":"Ignacio","family":"Camacho-Arroyo","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n en Reproducci\u00f3n Humana, Instituto Nacional de Perinatolog\u00eda-Facultad de Qu\u00edmica, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad de M\u00e9xico 11000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/S0140-6736(21)00389-5","article-title":"Endometriosis is a chronic systemic disease: Clinical challenges and novel innovation","volume":"397","author":"Taylor","year":"2021","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"154847","DOI":"10.1016\/j.prp.2023.154847","article-title":"Endometriosis: Classification, pathophysiology, and treatment options","volume":"251","author":"Pasalic","year":"2023","journal-title":"Pathol. 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