{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:51:04Z","timestamp":1785372664864,"version":"3.55.0"},"reference-count":76,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T00:00:00Z","timestamp":1753660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministry of Health, Italy","doi-asserted-by":"publisher","award":["RRC-2025-23686388"],"award-info":[{"award-number":["RRC-2025-23686388"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>About one billion people worldwide are affected by neurologic disorders. Among the various neurologic disorders, one of the most common is Alzheimer\u2019s disease (AD). AD is a neurodegenerative disorder that progressively affects cognitive functions, disrupting the daily lives of millions of individuals. Mild cognitive impairment (MCI) is often considered a prodromal stage of Alzheimer\u2019s disease. In this article, we retrieved data from the online available dataset GSE63060, which includes transcriptomic data of 329 blood samples, of which there are 104 cognitively normal controls, 80 MCI patients, and 145 AD patients. We used transcriptomic data related to all three groups to perform an over-representation analysis of the gene ontologies followed by a network analysis. The aim of our study is to pinpoint alterations, detectable through a non-invasive method, in biological processes affected in MCI that persist during AD. Our goal is to uncover transcriptomic changes that could support earlier diagnosis and the development of more effective therapeutic strategies, starting from the early stages of the disease, to slow down or mitigate its progression. Our work provides a consistent picture of the transcriptomic unbalance of many genes strongly involved in ribosomal formation and biogenesis and splicing processes both in patients with MCI and with AD.<\/jats:p>","DOI":"10.3390\/ijms26157303","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T16:00:37Z","timestamp":1753718437000},"page":"7303","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Early Dysregulation of RNA Splicing and Translation Processes Are Key Markers from Mild Cognitive Impairment to Alzheimer\u2019s Disease: An In Silico Transcriptomic Analysis"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1287-6400","authenticated-orcid":false,"given":"Simone","family":"D\u2019Angiolini","sequence":"first","affiliation":[{"name":"IRCCS Centro Neurolesi \u201cBonino-Pulejo\u201d, Contrada Casazza, Via Provinciale Palermo, 98124 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-3353","authenticated-orcid":false,"given":"Agnese","family":"Gugliandolo","sequence":"additional","affiliation":[{"name":"IRCCS Centro Neurolesi \u201cBonino-Pulejo\u201d, Contrada Casazza, Via Provinciale Palermo, 98124 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5281-2691","authenticated-orcid":false,"given":"Gabriella","family":"Cal\u00ec","sequence":"additional","affiliation":[{"name":"IRCCS Centro Neurolesi \u201cBonino-Pulejo\u201d, Contrada Casazza, Via Provinciale Palermo, 98124 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0358-7348","authenticated-orcid":false,"given":"Luigi","family":"Chiricosta","sequence":"additional","affiliation":[{"name":"IRCCS Centro Neurolesi \u201cBonino-Pulejo\u201d, Contrada Casazza, Via Provinciale Palermo, 98124 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.jalz.2007.04.381","article-title":"Forecasting the global burden of Alzheimer\u2019s disease","volume":"3","author":"Brookmeyer","year":"2007","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Murakami, S., and Lacayo, P. (2022). Biological and disease hallmarks of Alzheimer\u2019s disease defined by Alzheimer\u2019s disease genes. Front. Aging Neurosci., 14.","DOI":"10.3389\/fnagi.2022.996030"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"613","DOI":"10.4088\/JCP.v68n0419","article-title":"A high-density whole-genome association study reveals that APOE is the major susceptibility gene for sporadic late-onset Alzheimer\u2019s disease","volume":"68","author":"Coon","year":"2007","journal-title":"J. Clin. Psychiatry"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103715","DOI":"10.1016\/j.mcn.2022.103715","article-title":"The identification of PSEN1 p.Tyr159Ser mutation in a non-canonic early-onset Alzheimer\u2019s disease family","volume":"120","author":"Li","year":"2022","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1016\/j.neuron.2022.03.004","article-title":"ApoE Cascade Hypothesis in the pathogenesis of Alzheimer\u2019s disease and related dementias","volume":"110","author":"Martens","year":"2022","journal-title":"Neuron"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.jalz.2018.02.009","article-title":"Prevalence of the apolipoprotein E epsilon4 allele in amyloid beta positive subjects across the spectrum of Alzheimer\u2019s disease","volume":"14","author":"Mattsson","year":"2018","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.1073\/pnas.0305799101","article-title":"Involvement of oxidative stress-induced abnormalities in ceramide and cholesterol metabolism in brain aging and Alzheimer\u2019s disease","volume":"101","author":"Cutler","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1212\/01.wnl.0000263217.36439.da","article-title":"Inflammatory markers and the risk of Alzheimer disease: The Framingham Study","volume":"68","author":"Tan","year":"2007","journal-title":"Neurology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1136\/jnnp.74.9.1206","article-title":"The prevalence and causes of dementia in people under the age of 65 years","volume":"74","author":"Harvey","year":"2003","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.jalz.2016.01.012","article-title":"Molecular genetics of early-onset Alzheimer\u2019s disease revisited","volume":"12","author":"Cacace","year":"2016","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.jalz.2011.03.003","article-title":"Toward defining the preclinical stages of Alzheimer\u2019s disease: Recommendations from the National Institute on Aging-Alzheimer\u2019s Association workgroups on diagnostic guidelines for Alzheimer\u2019s disease","volume":"7","author":"Sperling","year":"2011","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jalz.2011.03.005","article-title":"The diagnosis of dementia due to Alzheimer\u2019s disease: Recommendations from the National Institute on Aging-Alzheimer\u2019s Association workgroups on diagnostic guidelines for Alzheimer\u2019s disease","volume":"7","author":"McKhann","year":"2011","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1001\/archneur.58.3.397","article-title":"Mild cognitive impairment represents early-stage Alzheimer disease","volume":"58","author":"Morris","year":"2001","journal-title":"Arch. Neurol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1474-4422(03)00262-X","article-title":"Mild cognitive impairment: Prevalence, prognosis, aetiology, and treatment","volume":"2","author":"DeCarli","year":"2003","journal-title":"Lancet Neurol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/S1474-4422(06)70355-6","article-title":"Association between CSF biomarkers and incipient Alzheimer\u2019s disease in patients with mild cognitive impairment: A follow-up study","volume":"5","author":"Hansson","year":"2006","journal-title":"Lancet Neurol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1002\/ana.20009","article-title":"Imaging brain amyloid in Alzheimer\u2019s disease with Pittsburgh Compound-B","volume":"55","author":"Klunk","year":"2004","journal-title":"Ann. Neurol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/s41582-024-00989-1","article-title":"Alzheimer disease blood biomarkers: Considerations for population-level use","volume":"20","author":"Mielke","year":"2024","journal-title":"Nat. Rev. Neurol."},{"key":"ref_18","first-page":"2","article-title":"Assessing the Preparedness of the Health Care System Infrastructure in Six European Countries for an Alzheimer\u2019s Treatment","volume":"8","author":"Hlavka","year":"2019","journal-title":"Rand Health Q."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1002\/alz.12470","article-title":"Expected wait times for access to a disease-modifying Alzheimer\u2019s treatment in the United States","volume":"18","author":"Mattke","year":"2022","journal-title":"Alzheimer\u2019s Dement. J. Alzheimer\u2019s Assoc."},{"key":"ref_20","first-page":"D433","article-title":"STRING: Known and predicted protein-protein associations, integrated and transferred across organisms","volume":"33","author":"Jensen","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"15","DOI":"10.2174\/156720510790274473","article-title":"Alzheimer\u2019s disease (AD) and Mild Cognitive Impairment (MCI) patients are characterized by increased BDNF serum levels","volume":"7","author":"Angelucci","year":"2010","journal-title":"Curr. Alzheimer Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1136\/bjsm.24.4.274","article-title":"Echocardiographic left ventricular hypertrophy in Chinese endurance athletes","volume":"24","author":"Lo","year":"1990","journal-title":"Br. J. Sports Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1002\/jcp.24604","article-title":"Ribosome biogenesis: Emerging evidence for a central role in the regulation of skeletal muscle mass","volume":"229","author":"Chaillou","year":"2014","journal-title":"J. Cell. Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2030","DOI":"10.1101\/gad.386106","article-title":"The transcriptional activity of RNA polymerase I is a key determinant for the level of all ribosome components","volume":"20","author":"Laferte","year":"2006","journal-title":"Genes Dev."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Freed, E.F., Prieto, J.L., McCann, K.L., McStay, B., and Baserga, S.J. (2012). NOL11, implicated in the pathogenesis of North American Indian childhood cirrhosis, is required for pre-rRNA transcription and processing. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002892"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lee, L., and Whittall, J.B. (2025). WDR75: An essential protein for ribosome assembly undergoing purifying selection. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0318395"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/wrna.57","article-title":"The small subunit processome in ribosome biogenesis-progress and prospects","volume":"2","author":"Phipps","year":"2011","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1007\/s13238-018-0526-7","article-title":"Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate","volume":"10","author":"Zhou","year":"2019","journal-title":"Protein Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"113280","DOI":"10.1016\/j.celrep.2023.113280","article-title":"ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells","volume":"42","author":"Hao","year":"2023","journal-title":"Cell Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/S1097-2765(02)00438-0","article-title":"The sigma(70)-like motif: A eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis","volume":"9","author":"Wehner","year":"2002","journal-title":"Mol. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1093\/nar\/gkz105","article-title":"Sas10 controls ribosome biogenesis by stabilizing Mpp10 and delivering the Mpp10-Imp3-Imp4 complex to nucleolus","volume":"47","author":"Zhao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1091\/mbc.E15-02-0073","article-title":"The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis","volume":"26","author":"Zorbas","year":"2015","journal-title":"Mol. Biol. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Rahman, M.L., Bonnard, A.A., Wang, F., Ruaud, L., Guimiot, F., Li, Y., Defer, I., Wang, Y., Marchand, V., and Motorin, Y. (2024). New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops. medRxiv.","DOI":"10.1101\/2024.08.26.24312490"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1083\/jcb.200501141","article-title":"Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation","volume":"170","author":"Holzel","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1038\/s41586-022-05621-0","article-title":"Principles of mitoribosomal small subunit assembly in eukaryotes","volume":"614","author":"Harper","year":"2023","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1750-1326-4-5","article-title":"Independent component analysis of Alzheimer\u2019s DNA microarray gene expression data","volume":"4","author":"Kong","year":"2009","journal-title":"Mol. Neurodegener."},{"key":"ref_37","first-page":"2003","article-title":"Oxidative stress, mitochondrial damage and neurodegenerative diseases","volume":"8","author":"Guo","year":"2013","journal-title":"Neural Regen. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/ng909","article-title":"Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA","volume":"31","author":"Falkenberg","year":"2002","journal-title":"Nat. Genet."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.cmet.2018.05.007","article-title":"Ubiquitination of ABCE1 by NOT4 in Response to Mitochondrial Damage Links Co-translational Quality Control to PINK1-Directed Mitophagy","volume":"28","author":"Wu","year":"2018","journal-title":"Cell Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/onc.2012.604","article-title":"The function of ORAOV1\/LTO1, a gene that is overexpressed frequently in cancer: Essential roles in the function and biogenesis of the ribosome","volume":"33","author":"Zhai","year":"2014","journal-title":"Oncogene"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/sj.emboj.7600541","article-title":"Biogenesis of cytosolic ribosomes requires the essential iron-sulphur protein Rli1p and mitochondria","volume":"24","author":"Kispal","year":"2005","journal-title":"EMBO J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e08231","DOI":"10.7554\/eLife.08231","article-title":"The deca-GX3 proteins Yae1-Lto1 function as adaptors recruiting the ABC protein Rli1 for iron-sulfur cluster insertion","volume":"4","author":"Paul","year":"2015","journal-title":"eLife"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nature12731","article-title":"Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export","volume":"505","author":"Matsuo","year":"2014","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1534\/genetics.110.115584","article-title":"Dominant mutations in the late 40S biogenesis factor Ltv1 affect cytoplasmic maturation of the small ribosomal subunit in Saccharomyces cerevisiae","volume":"185","author":"Fassio","year":"2010","journal-title":"Genetics"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2422","DOI":"10.1128\/MCB.05343-11","article-title":"Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response","volume":"31","author":"Dosil","year":"2011","journal-title":"Mol. Cell. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4351","DOI":"10.1007\/s13277-015-4062-2","article-title":"RRP12 is a crucial nucleolar protein that regulates p53 activity in osteosarcoma cells","volume":"37","author":"Choi","year":"2016","journal-title":"Tumour Biol. J. Int. Soc. Oncodev. Biol. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1038\/nrm2838","article-title":"The mechanism of eukaryotic translation initiation and principles of its regulation","volume":"11","author":"Jackson","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nature13401","article-title":"DENR-MCT-1 promotes translation re-initiation downstream of uORFs to control tissue growth","volume":"512","author":"Schleich","year":"2014","journal-title":"Nature"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1111\/bpa.12335","article-title":"Altered Machinery of Protein Synthesis in Alzheimer\u2019s: From the Nucleolus to the Ribosome","volume":"26","author":"Gil","year":"2016","journal-title":"Brain Pathol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Will, C.L., and Luhrmann, R. (2011). Spliceosome structure and function. Cold Spring Harb. Perspect. Biol., 3.","DOI":"10.1101\/cshperspect.a003707"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1101\/gad.13.7.841","article-title":"Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing","volume":"13","author":"Chua","year":"1999","journal-title":"Genes Dev."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"4978","DOI":"10.1093\/emboj\/cdf480","article-title":"Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein","volume":"21","author":"Will","year":"2002","journal-title":"EMBO J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.cell.2008.09.020","article-title":"An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs","volume":"135","author":"Chari","year":"2008","journal-title":"Cell"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"110539","DOI":"10.1016\/j.ygeno.2022.110539","article-title":"FRG1 is a direct transcriptional regulator of nonsense-mediated mRNA decay genes","volume":"115","author":"Palo","year":"2023","journal-title":"Genomics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/nsmb.1415","article-title":"Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4\/U6-U5 tri-snRNP into the spliceosome","volume":"15","author":"Mathew","year":"2008","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"108989","DOI":"10.1016\/j.celrep.2021.108989","article-title":"Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms","volume":"35","author":"Daniels","year":"2021","journal-title":"Cell Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"8192","DOI":"10.1038\/ncomms9192","article-title":"mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3","volume":"6","author":"Gebhardt","year":"2015","journal-title":"Nat. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1261\/rna.037069.112","article-title":"The nuclear cap-binding complex interacts with the U4\/U6.U5 tri-snRNP and promotes spliceosome assembly in mammalian cells","volume":"19","author":"Pabis","year":"2013","journal-title":"Rna"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/nrm.2015.15","article-title":"The regulation and functions of the nuclear RNA exosome complex","volume":"17","author":"Kilchert","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.molcel.2016.09.025","article-title":"Identification of a Nuclear Exosome Decay Pathway for Processed Transcripts","volume":"64","author":"Meola","year":"2016","journal-title":"Mol. Cell"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1016\/j.celrep.2019.11.093","article-title":"Dysregulation of RNA Splicing in Tauopathies","volume":"29","author":"Apicco","year":"2019","journal-title":"Cell Rep."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1046\/j.0953-816x.2001.01836.x","article-title":"Characterizing CGI-94 (comparative gene identification-94) which is down-regulated in the hippocampus of early stage Alzheimer\u2019s disease brain","volume":"15","author":"Heese","year":"2002","journal-title":"Eur. J. Neurosci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1101\/gad.1569307","article-title":"Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes","volume":"21","author":"Zhang","year":"2007","journal-title":"Genes Dev."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Ibanez-Cabellos, J.S., Seco-Cervera, M., Picher-Latorre, C., Perez-Machado, G., Garcia-Gimenez, J.L., and Pallardo, F.V. (2020). Acute depletion of telomerase components DKC1 and NOP10 induces oxidative stress and disrupts ribosomal biogenesis via NPM1 and activation of the P53 pathway. Biochim. Biophys. Acta Mol. Cell Res., 1867.","DOI":"10.1016\/j.bbamcr.2020.118845"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1038\/s43587-022-00290-0","article-title":"Alzheimer\u2019s disease-associated U1 snRNP splicing dysfunction causes neuronal hyperexcitability and cognitive impairment","volume":"2","author":"Chen","year":"2022","journal-title":"Nat. Aging"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"102769","DOI":"10.1016\/j.dnarep.2019.102769","article-title":"The splicing component ISY1 regulates APE1 in base excision repair","volume":"86","author":"Jaiswal","year":"2020","journal-title":"DNA Repair"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1080\/15476286.2014.996494","article-title":"Splicing changes in SMA mouse motoneurons and SMN-depleted neuroblastoma cells: Evidence for involvement of splicing regulatory proteins","volume":"11","author":"Huo","year":"2014","journal-title":"RNA Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1523\/JNEUROSCI.3029-15.2016","article-title":"Pathological Tau Promotes Neuronal Damage by Impairing Ribosomal Function and Decreasing Protein Synthesis","volume":"36","author":"Meier","year":"2016","journal-title":"J. Neurosci. Off. J. Soc. Neurosci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"D711","DOI":"10.1093\/nar\/gky964","article-title":"ArrayExpress update\u2014From bulk to single-cell expression data","volume":"47","author":"Athar","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1093\/nar\/30.1.207","article-title":"Gene Expression Omnibus: NCBI gene expression and hybridization array data repository","volume":"30","author":"Edgar","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Sood, S., Gallagher, I.J., Lunnon, K., Rullman, E., Keohane, A., Crossland, H., Phillips, B.E., Cederholm, T., Jensen, T., and van Loon, L.J. (2015). A novel multi-tissue RNA diagnostic of healthy ageing relates to cognitive health status. Genome Biol., 16.","DOI":"10.1186\/s13059-015-0750-x"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Gentleman, R.C., Carey, V.J., Bates, D.M., Bolstad, B., Dettling, M., Dudoit, S., Ellis, B., Gautier, L., Ge, Y., and Gentry, J. (2004). Bioconductor: Open software development for computational biology and bioinformatics. Genome Biol., 5.","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3292","DOI":"10.1038\/s41596-024-01020-z","article-title":"Using clusterProfiler to characterize multiomics data","volume":"19","author":"Xu","year":"2024","journal-title":"Nat. Protoc."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"12515","DOI":"10.1093\/nar\/gkac1035","article-title":"Structure of Escherichia coli heat shock protein Hsp15 in complex with the ribosomal 50S subunit bearing peptidyl-tRNA","volume":"50","author":"Safdari","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A software environment for integrated models of biomolecular interaction networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/15\/7303\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:17:35Z","timestamp":1760033855000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/15\/7303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,28]]},"references-count":76,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["ijms26157303"],"URL":"https:\/\/doi.org\/10.3390\/ijms26157303","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,28]]}}}