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For instance, ArfGAP with dual pleckstrin homology domains 1 (Adap1) exerts an anti-hypertrophic effect in cultured cardiomyocytes. Its homologous protein, Adap2, is also expressed in the heart but its role remains elusive. To elucidate its function, we investigated the effects of adenoviral-mediated overexpression of Adap2 in cultured neonatal rat ventricular myocytes under both basal and pro-hypertrophic conditions, employing a range of microscopy and biochemical techniques. Despite minimal detection in neonatal rat hearts, Adap2 was found to be well expressed in adult rat hearts, being predominantly localized at the membrane fraction. In contrast to Adap1, overexpression of Adap2 provokes the robust accumulation of \u03b21-integrin at the cellular surface of cultured cardiomyocytes. Interestingly, overexpressed Adap2 relocalizes at the sarcolemma and increases the size of cardiomyocytes upon phenylephrine stimulation, despite attenuating Erk1\/2 phosphorylation and Nppa gene expression. Under these same conditions, cardiomyocytes overexpressing Adap2 also express higher level of detyrosinated tubulin, a marker of hypertrophic response. These findings provide new insights into the pro-hypertrophic function of Adap2 in cardiomyocytes.<\/jats:p>","DOI":"10.3390\/ijms26157588","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T07:45:11Z","timestamp":1754466311000},"page":"7588","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["ArfGAP with Dual Pleckstrin Homology Domains 2 Promotes Hypertrophy of Cultured Neonatal Cardiomyocytes"],"prefix":"10.3390","volume":"26","author":[{"given":"Jonathan","family":"Berthiaume","sequence":"first","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey-Ann","family":"Dumont","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7681-6212","authenticated-orcid":false,"given":"Lauralyne","family":"Dumont","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Fr\u00e9d\u00e9rique","family":"Roy","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7193-0051","authenticated-orcid":false,"given":"Hugo","family":"Gigu\u00e8re","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8600-5440","authenticated-orcid":false,"given":"Mannix","family":"Auger-Messier","sequence":"additional","affiliation":[{"name":"D\u00e9partement de M\u00e9decine\u2014Service de Cardiologie, Centre de Recherche du CHUS, Facult\u00e9 de M\u00e9decine et des Sciences de la Sant\u00e9, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"},{"name":"Institut de Pharmacologie de Sherbrooke, Universit\u00e9 de Sherbrooke, Sherbrooke, QC J1H 5N4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Giguere, H., Dumont, A.A., Berthiaume, J., Oliveira, V., Laberge, G., and Auger-Messier, M. 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