{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T15:55:14Z","timestamp":1784735714448,"version":"3.55.0"},"reference-count":72,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T00:00:00Z","timestamp":1754438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Clinic for Gynecology, Obstetrics and Reproductive Medicine, Saarland University Clinic"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sperm count, motility, and morphology are semen parameters that directly affect male fertility. The presence of cytokines in seminal plasma negatively or positively influences these parameters. Interleukins and prostaglandins are proinflammatory cytokines present in human seminal plasma and play crucial roles in fertilization, in general and after intracytoplasmic sperm injection (ICSI) procedures. This study aimed to investigate the possible influence of interleukins IL-17 and IL-18, and prostaglandins PGE2 and PGF2\u03b1 on male infertility. Semen samples were collected from 58 males who underwent the ICSI procedure. An enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of IL-17, IL-18, PGE2, and PGF2\u03b1, and these concentrations were then correlated with semen parameters and the rate of fertilization. Furthermore, the chromatin integrity of the sperm was evaluated with an Acridine Orange (AO) assay. The results showed an inversely proportional relationship between the AO binding intensity and fertilization rate (r = \u22120.394; p \u2264 0.002). Furthermore, a negative correlation was observed between the IL-18 concentration and positive AO (p \u2264 0.021). Moreover, the IL-18 concentration was positively correlated with the fertilization rate (p \u2264 0.05). In contrast, IL-17 did not significantly correlate with any semen parameters or with the fertilization rate. Seminal PGE2 levels were significantly correlated with embryo cleavage at 72 h (p \u2264 0.05). To conclude, this study revealed that denaturation of sperm nuclear deoxyribonucleic acid (DNA) contributes to low fertilization rates. In addition, this study proposed a potential role for IL-18 in fertilization. PGE2 likely influences embryo development, but further studies are needed to examine the impact of seminal PGE2 on the oocyte to fully elucidate its contribution to this complex biological process.<\/jats:p>","DOI":"10.3390\/ijms26157627","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T08:33:06Z","timestamp":1754555586000},"page":"7627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Relationship Between Prostaglandin and Interleukin Concentrations in Seminal Fluid and Their Influence on the Rate of Fertilization in Men Undergoing ICSI"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-8836","authenticated-orcid":false,"given":"Houda","family":"Amor","sequence":"first","affiliation":[{"name":"Biochemistry & Molecular Biology of Reproductive Medicine Laboratory, Department of Obstetrics, Gynecology & Reproductive Medicine, Medical Faculty, University of Saarland, 66421 Homburg, Germany"},{"name":"Department of Endocrinology & Reproductive Medicine, University Clinics Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2673-2691","authenticated-orcid":false,"given":"Fatina W.","family":"Dahadhah","sequence":"additional","affiliation":[{"name":"Department of Basic Dental Sciences, Faculty of Dentistry, The Hashemite University, Zarqa 13115, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Michael","family":"Jankowski","sequence":"additional","affiliation":[{"name":"Biochemistry & Molecular Biology of Reproductive Medicine Laboratory, Department of Obstetrics, Gynecology & Reproductive Medicine, Medical Faculty, University of Saarland, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rami","family":"Al Nasser","sequence":"additional","affiliation":[{"name":"British Syrian IVF Centre, Al-Rasheed Hospital, Damascus 0100, Syria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Endocrinology & Reproductive Medicine, University Clinics Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingolf","family":"Juhasz-B\u00f6ss","sequence":"additional","affiliation":[{"name":"Department of Endocrinology & Reproductive Medicine, University Clinics Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich Franz","family":"Solomayer","sequence":"additional","affiliation":[{"name":"Biochemistry & Molecular Biology of Reproductive Medicine Laboratory, Department of Obstetrics, Gynecology & Reproductive Medicine, Medical Faculty, University of Saarland, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamad Eid","family":"Hammadeh","sequence":"additional","affiliation":[{"name":"Biochemistry & Molecular Biology of Reproductive Medicine Laboratory, Department of Obstetrics, Gynecology & Reproductive Medicine, Medical Faculty, University of Saarland, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/S0015-0282(02)03105-9","article-title":"Best practice policies for male infertility","volume":"77","author":"Sharlip","year":"2002","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1093\/humupd\/6.2.160","article-title":"Sperm function assays and their predictive value for fertilization outcome in IVF therapy: A meta-analysis","volume":"6","author":"Oehninger","year":"2000","journal-title":"Hum. Reprod. Update"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1002\/j.1939-4640.2000.tb03273.x","article-title":"DNA integrity in human spermatozoa: Relationships with semen quality","volume":"21","author":"Irvine","year":"2000","journal-title":"J. Androl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1530\/ror.0.0040031","article-title":"Origin of DNA damage in ejaculated human spermatozoa","volume":"4","author":"Sakkas","year":"1999","journal-title":"Rev. Reprod."},{"key":"ref_5","unstructured":"WHO World Health Organization (2021). WHO laboratory manual for the examination and processing of human semen. WHO Laboratory Manual for the Examination and Processing of Human Semen, World Health Organization."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1002\/1098-2795(20010401)58:4<357::AID-MRD1>3.0.CO;2-T","article-title":"Transition nuclear proteins during spermiogenesis: Unrepaired DNA breaks not allowed","volume":"58","author":"Kierszenbaum","year":"2001","journal-title":"Mol. Reprod. Dev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/humupd\/dml009","article-title":"Protamines and male infertility","volume":"12","author":"Oliva","year":"2006","journal-title":"Hum. Reprod. Update"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1093\/humupd\/dmg027","article-title":"Role of sperm chromatin abnormalities and DNA damage in male infertility","volume":"9","author":"Agarwal","year":"2003","journal-title":"Hum. Reprod. Update"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1093\/humrep\/15.5.1112","article-title":"The use of two density gradient centrifugation techniques and the swim-up method to separate spermatozoa with chromatin and nuclear DNA anomalies","volume":"15","author":"Sakkas","year":"2000","journal-title":"Hum. Reprod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.fertnstert.2009.02.050","article-title":"DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome","volume":"94","author":"Franchi","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1093\/oxfordjournals.humrep.a137472","article-title":"Use of aniline blue to assess chromatin condensation in morphologically normal spermatozoa in normal and infertile men","volume":"6","author":"Hofmann","year":"1991","journal-title":"Hum. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/S0090-4295(02)01975-1","article-title":"Prevalence of abnormal sperm DNA denaturation in fertile and infertile men","volume":"60","author":"Zini","year":"2002","journal-title":"Urology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1093\/humrep\/den321","article-title":"Sperm DNA damage is associated with an increased risk of pregnancy loss after IVF and ICSI: Systematic review and meta-analysis","volume":"23","author":"Zini","year":"2008","journal-title":"Hum. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1093\/humrep\/deh233","article-title":"Full-term pregnancies achieved with ICSI despite high levels of sperm chromatin damage","volume":"19","author":"Gandini","year":"2004","journal-title":"Hum. Reprod."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.fertnstert.2007.06.018","article-title":"Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates","volume":"90","author":"Lin","year":"2008","journal-title":"Fertil. Steril."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"536","DOI":"10.2164\/jandrol.110.012583","article-title":"Seminal plasma: An essential attribute to spermatozoa","volume":"33","author":"Juyena","year":"2012","journal-title":"J. Androl."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1002\/pros.10255","article-title":"Proteomic analysis of human prostasomes","volume":"56","author":"Utleg","year":"2003","journal-title":"Prostate"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Pilch, B., and Mann, M. (2006). Large-scale and high-confidence proteomic analysis of human seminal plasma. Genome Biol., 7.","DOI":"10.1186\/gb-2006-7-5-r40"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1093\/molehr\/gam028","article-title":"Seminal plasma differentially regulates inflammatory cytokine gene expression in human cervical and vaginal epithelial cells","volume":"13","author":"Sharkey","year":"2007","journal-title":"Mol. Hum. Reprod."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1093\/humupd\/dmu052","article-title":"The role of seminal plasma for improved outcomes during in vitro fertilization treatment: Review of the literature and meta-analysis","volume":"21","author":"Crawford","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"ref_21","first-page":"106","article-title":"The role of the microbial factor in the pathogenesis of male infertility","volume":"2","author":"Bukharin","year":"2000","journal-title":"Zh. Mikrobiol. Epidemiol. Immunobiol."},{"key":"ref_22","first-page":"123","article-title":"Impact of inflammation on male reproductive tract","volume":"16","author":"Azenabor","year":"2015","journal-title":"J. Reprod. Infertil."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"N3","DOI":"10.1097\/00003246-200004001-00002","article-title":"Cytokine signaling-regulation of the immune response in normal and critically ill states","volume":"28","author":"Oberholzer","year":"2000","journal-title":"Crit. Care Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"325","DOI":"10.2164\/jandrol.106.001149","article-title":"Inflammatory mediators exert toxic effects of oxidative stress on human spermatozoa","volume":"28","author":"Fraczek","year":"2007","journal-title":"J. Androl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1111\/j.1439-0272.2010.01042.x","article-title":"Cytokine expression in the seminal plasma and its effects on fertilisation rates in an IVF cycle","volume":"43","author":"Seshadri","year":"2011","journal-title":"Andrologia"},{"key":"ref_26","first-page":"291","article-title":"Proinflammatory cytokines","volume":"1","author":"Feldmann","year":"2001","journal-title":"Cytokine Ref."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0165-0378(01)00063-8","article-title":"A repertoire of cytokines in human seminal plasma","volume":"54","author":"Maegawa","year":"2002","journal-title":"J. Reprod. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0165-0378(02)00060-8","article-title":"Cytokines and the immune-testicular axis","volume":"58","author":"Hedger","year":"2003","journal-title":"J. Reprod. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1093\/humrep\/16.3.517","article-title":"Relationship of seminal plasma interleukin (IL)-8 and IL-6 with semen quality","volume":"16","author":"Boit","year":"2001","journal-title":"Hum. Reprod."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1046\/j.1442-2042.2001.00358.x","article-title":"Seminal plasma cytokine levels in the diagnosis of chronic pelvic pain syndrome","volume":"8","author":"Orhan","year":"2001","journal-title":"Int. J. Urol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1016\/S0015-0282(16)58643-9","article-title":"Is leukocytospermia clinically relevant?","volume":"66","author":"Yanushpolsky","year":"1996","journal-title":"Fertil. Steril."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1093\/oxfordjournals.humrep.a137493","article-title":"Influence of polymorphonuclear granulocytes on the zona-free hamster oocyte assay","volume":"6","author":"Vogelpoel","year":"1991","journal-title":"Hum. Reprod."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Loveland, K.L., Klein, B., Pueschl, D., Indumathy, S., Bergmann, M., Loveland, B.E., Hedger, M.P., and Schuppe, H.-C. (2017). Cytokines in Male Fertility and Reproductive Pathologies: Immunoregulation and Beyond. Front. Endocrinol., 8.","DOI":"10.3389\/fendo.2017.00307"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mary, A.P., Nandeesha, H., Papa, D., Chitra, T., Ganesh, R.N., and Menon, V. (2021). Matrix metalloproteinase-9 is elevated and related to interleukin-17 and psychological stress in male infertility: A cross-sectional study. Int. J. Reprod. Biomed., 19.","DOI":"10.18502\/ijrm.v19i4.9059"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ihim, S.A., Abubakar, S.D., Zian, Z., Sasaki, T., Saffarioun, M., Maleknia, S., and Azizi, G. (2022). Interleukin-18 cytokine in immunity, inflammation, and autoimmunity: Biological role in induction, regulation, and treatment. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.919973"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1111\/j.1600-0897.2006.00380.x","article-title":"Increased IL-18 levels in seminal plasma of infertile men with genital tract infections","volume":"55","author":"Matalliotakis","year":"2006","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ijbiomac.2013.12.005","article-title":"IL-18 levels in the semen of male infertility: Semen analysis","volume":"64","author":"Qian","year":"2014","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jri.2016.03.006","article-title":"The relationship of seminal transforming growth factor-\u03b21 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF\/ICSI treatment","volume":"117","author":"Nikolaeva","year":"2016","journal-title":"J. Reprod. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.tem.2004.08.006","article-title":"Prostaglandin receptor signalling and function in human endometrial pathology","volume":"15","author":"Jabbour","year":"2004","journal-title":"Trends Endocrinol. Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1152\/physrev.1999.79.4.1193","article-title":"Prostanoid receptors: Structures, properties, and functions","volume":"79","author":"Narumiya","year":"1999","journal-title":"Physiol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0163-7827(01)00002-9","article-title":"Mammalian phospholipases A 2: Mediators of inflammation, proliferation and apoptosis","volume":"40","author":"Capper","year":"2001","journal-title":"Prog. Lipid Res."},{"key":"ref_42","unstructured":"Patton, K.T., Thibodeau, G.A., and Douglas, M.M. (2012). Essentials of Anatomy & Physiology, Elsevier."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1095\/biolreprod58.2.600","article-title":"Lipocalin-type prostaglandin D synthase in human male reproductive organs and seminal plasma","volume":"58","author":"Tokugawa","year":"1998","journal-title":"Biol. Reprod."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1093\/molehr\/4.6.555","article-title":"Prostaglandins induce calcium influx in human spermatozoa","volume":"4","author":"Shimizu","year":"1998","journal-title":"Mol. Hum. Reprod."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1071\/RD14035","article-title":"Low physiological levels of prostaglandins E2 and F2\u03b1 improve human sperm functions","volume":"28","author":"Rios","year":"2016","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Szczuko, M., Kikut, J., Komorniak, N., Bilicki, J., Celewicz, Z., and Zi\u0119tek, M. (2020). The Role of Arachidonic and Linoleic Acid Derivatives in Pathological Pregnancies and the Human Reproduction Process. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21249628"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Male, D., Brostoff, J., Roth, D.B., and Roit, I. (2006). Mechanism of Innate Immunity. Immunology, Mosby.","DOI":"10.1016\/B0-323-03399-7\/50008-8"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/S0015-0282(00)01591-0","article-title":"Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa","volume":"74","author":"Duru","year":"2000","journal-title":"Fertil. Steril."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Sanocka, D., and Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reprod. Biol. Endocrinol., 2.","DOI":"10.1186\/1477-7827-2-12"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1080\/01485010290099390","article-title":"Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss","volume":"49","author":"Carrell","year":"2003","journal-title":"Arch. Androl."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/j.1365-2605.2008.00943.x","article-title":"Biological and clinical significance of DNA damage in the male germ line","volume":"32","author":"Aitken","year":"2009","journal-title":"Int. J. Androl."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.theriogenology.2005.09.011","article-title":"Clinical aspects of sperm DNA fragmentation detection and male infertility","volume":"65","author":"Evenson","year":"2006","journal-title":"Theriogenology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1080\/19396360801957739","article-title":"Sperm DNA tests as useful adjuncts to semen analysis","volume":"54","author":"Lewis","year":"2008","journal-title":"Syst. Biol. Reprod. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1093\/humrep\/15.8.1717","article-title":"Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques","volume":"15","author":"Larson","year":"2000","journal-title":"Hum. Reprod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1439-0272.1998.tb01379.x","article-title":"Association between sperm cell chromatin condensation, morphology based on strict criteria, and fertilization, cleavage and pregnancy rates in an IVF program","volume":"30","author":"Hammadeh","year":"1998","journal-title":"Andrologia"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s11195-006-9015-7","article-title":"Impact of sperm collection methods on sperm parameters in spinal cord injured men and compared to normal controls in ICSI program","volume":"24","author":"Salsabili","year":"2006","journal-title":"Sex. Disabil."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1023\/A:1016006509036","article-title":"Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos","volume":"19","author":"Tomazevic","year":"2002","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.fertnstert.2003.09.063","article-title":"Sperm chromatin structure assay (SCSA\u00ae) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles","volume":"81","author":"Virro","year":"2004","journal-title":"Fertil. Steril."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s00441-005-1127-3","article-title":"Seminal plasma and male factor signalling in the female reproductive tract","volume":"322","author":"Robertson","year":"2005","journal-title":"Cell Tissue Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1365-2605.2005.00547.x","article-title":"Consequences of semen inflammation and lipid peroxidation on fertilization capacity of spermatozoa in in vitro conditions","volume":"28","author":"Pawelczyk","year":"2005","journal-title":"Int. J. Androl."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"617","DOI":"10.3109\/08820139.2014.909453","article-title":"IL-17A concentration of seminal plasma and follicular fluid in infertile men and women with various clinical diagnoses","volume":"43","author":"Sabbaghi","year":"2014","journal-title":"Immunol. Investig."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.4049\/jimmunol.161.7.3400","article-title":"IL-12 up-regulates IL-18 receptor expression on T cells, Th1 cells, and B cells: Synergism with IL-18 for IFN-\u03b3 production","volume":"161","author":"Yoshimoto","year":"1998","journal-title":"J. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1093\/humrep\/deh324","article-title":"A combination of interleukin-6 and its soluble receptor impairs sperm motility: Implications in infertility associated with endometriosis","volume":"19","author":"Yoshida","year":"2004","journal-title":"Hum. Reprod."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Bungum, M. (2012). Sperm DNA integrity assessment: A new tool in diagnosis and treatment of fertility. Obstet. Gynecol. Int., 2012.","DOI":"10.1155\/2012\/531042"},{"key":"ref_65","first-page":"5672","article-title":"The relationship between IL-17 and male infertility: Semen analysis","volume":"6","author":"Qian","year":"2012","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1600-0897.1996.tb00145.x","article-title":"Quantitative Assessment of Cytokines (GRO\u03b1 and IL-10) in Human Seminal Plasma During Genitourinary Inflammation","volume":"36","author":"Rajasekaran","year":"1996","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1093\/jb\/mvu081","article-title":"Roles of prostaglandin receptors in female reproduction","volume":"157","author":"Sugimoto","year":"2015","journal-title":"J. Biochem."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1152\/physiol.00036.2010","article-title":"Regulation of smooth muscle contraction by the epithelium: Role of prostaglandins","volume":"26","author":"Ruan","year":"2011","journal-title":"Physiology"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/0090-6980(81)90199-4","article-title":"The influence of prostaglandins on sperm motility","volume":"21","author":"Schlegel","year":"1981","journal-title":"Prostaglandins"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/S0015-0282(16)59723-4","article-title":"Effect of prostaglandins on human sperm function in vitro and seminal adenosine triphosphate content","volume":"49","author":"Gottlieb","year":"1988","journal-title":"Fertil. Steril."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"14539","DOI":"10.1073\/pnas.0805699105","article-title":"Timely interaction between prostaglandin and chemokine signaling is a prerequisite for successful fertilization","volume":"105","author":"Tamba","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1002\/eji.200838969","article-title":"Prostaglandin E2 enhances Th17 responses via modulation of IL-17 and IFN-\u03b3 production by memory CD4+ T cells","volume":"39","author":"Napolitani","year":"2009","journal-title":"Eur. J. 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