{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:48:31Z","timestamp":1790455711290,"version":"4.1.0"},"reference-count":79,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Mohammed VI Polytechnic University (UM6P) of Benguerir"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The phytochemical profiles, antioxidant capacities, mineral composition, and antibacterial activities of Zingiber officinale (Z. officinal) and Piper nigrum (P. nigrum) were explored through aqueous, ethanolic, and methanolic extractions. The extracts were analyzed for polyphenols, flavonoids, and tannins, and their antioxidant potential was assessed using the DPPH assay. UPLC-HRMS identified major bioactive compounds, including 6-gingerol and shogaol in Z. officinale, and piperine and piperlonguminine in P. nigrum. Mineral analysis showed that P. nigrum was particularly rich in essential elements, including calcium (Ca), magnesium (Mg), and iron (Fe). In antibacterial testing, P. nigrum demonstrated wider zones of inhibition against E. coli, whereas Z. officinale was more active at lower concentrations, showing MICs as low as 3.91 \u00b5g\/mL against Salmonella and S. aureus. PCA analysis revealed strong correlations between phenolic content and biological effects. These results underscore the potential of both spices as effective natural agents for use in food preservation and health-promoting applications.<\/jats:p>","DOI":"10.3390\/ijms26167782","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T07:45:31Z","timestamp":1755071131000},"page":"7782","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrated Phytochemical Profiling, UPLC-HRMS Characterization, and Bioactivity Evaluation of Zingiber officinale and Piper nigrum"],"prefix":"10.3390","volume":"26","author":[{"given":"Aicha","family":"Boubker","sequence":"first","affiliation":[{"name":"Laboratory of Materials, Nanotechnologies and Environment, Faculty of Sciences, Mohammed V University in Rabat, BP: 1014, Ibn Battouta Avenue, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmoula","family":"El Ouardi","sequence":"additional","affiliation":[{"name":"Department of Microbiology of Water, Food and Environment, National Institute of Hygiene, BP: 769, Agdal, 27, Avenue Ibn Batouta, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5027-1173","authenticated-orcid":false,"given":"Taha","family":"El Kamli","sequence":"additional","affiliation":[{"name":"Laboratory of Anti-Doping Control, Hassan II Institute of Agronomic and Veterinary, BP: 6202, Madinat Al Irfane, Rabat 10101, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Kaicer","sequence":"additional","affiliation":[{"name":"Laboratory of Analysis, Geometry and Applications, Systems and Optimization, Ibn Tofail University, Campus of University, BP: 242, Kenitra 14000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1147-9128","authenticated-orcid":false,"given":"Faouzi","family":"Kichou","sequence":"additional","affiliation":[{"name":"Department of Pathology and Veterinary Public Health, Hassan II Institute of Agronomic and Veterinary, BP: 6202, Madinat Al Irfane, Rabat 10101, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khaoula","family":"Errafii","sequence":"additional","affiliation":[{"name":"African Genom, University Mohammed VI Polytechnic Center, BP: 660, Hay Moulay Rachid, Benguerir 43150, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1386-0806","authenticated-orcid":false,"given":"Adnane","family":"El Hamidi","sequence":"additional","affiliation":[{"name":"Laboratory of Materials, Nanotechnologies and Environment, Faculty of Sciences, Mohammed V University in Rabat, BP: 1014, Ibn Battouta Avenue, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachid","family":"Ben Aakame","sequence":"additional","affiliation":[{"name":"Laboratory of Food Toxicology, National Institute of Hygiene, BP: 769, Agdal, 27, Avenue Ibn Batouta, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8120-546X","authenticated-orcid":false,"given":"Aicha","family":"Sifou","sequence":"additional","affiliation":[{"name":"Laboratory of Materials, Nanotechnologies and Environment, Faculty of Sciences, Mohammed V University in Rabat, BP: 1014, Ibn Battouta Avenue, Rabat 10000, Morocco"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jtcme.2024.05.006","article-title":"Medicinal Plants for the Management of Post-COVID-19 Fatigue: A Literature Review on the Role and Mechanisms","volume":"15","author":"Chen","year":"2025","journal-title":"J. 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