{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:03:54Z","timestamp":1787065434509,"version":"build-2736575974"},"reference-count":43,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Fujian Province","award":["2025J01929"],"award-info":[{"award-number":["2025J01929"]}]},{"name":"Natural Science Foundation of Fujian Province","award":["2022J01236"],"award-info":[{"award-number":["2022J01236"]}]},{"name":"Natural Science Foundation of Fujian Province","award":["XRCXZ2021030"],"award-info":[{"award-number":["XRCXZ2021030"]}]},{"name":"Natural Science Foundation of Fujian Province","award":["X2024005-talents"],"award-info":[{"award-number":["X2024005-talents"]}]},{"name":"Startup Fund for High-level Talents of the Fujian Medical University","award":["2025J01929"],"award-info":[{"award-number":["2025J01929"]}]},{"name":"Startup Fund for High-level Talents of the Fujian Medical University","award":["2022J01236"],"award-info":[{"award-number":["2022J01236"]}]},{"name":"Startup Fund for High-level Talents of the Fujian Medical University","award":["XRCXZ2021030"],"award-info":[{"award-number":["XRCXZ2021030"]}]},{"name":"Startup Fund for High-level Talents of the Fujian Medical University","award":["X2024005-talents"],"award-info":[{"award-number":["X2024005-talents"]}]},{"name":"Scientific Research Foundation for the High-level Talents, Fujian University of Traditional Chinese Medicine","award":["2025J01929"],"award-info":[{"award-number":["2025J01929"]}]},{"name":"Scientific Research Foundation for the High-level Talents, Fujian University of Traditional Chinese Medicine","award":["2022J01236"],"award-info":[{"award-number":["2022J01236"]}]},{"name":"Scientific Research Foundation for the High-level Talents, Fujian University of Traditional Chinese Medicine","award":["XRCXZ2021030"],"award-info":[{"award-number":["XRCXZ2021030"]}]},{"name":"Scientific Research Foundation for the High-level Talents, Fujian University of Traditional Chinese Medicine","award":["X2024005-talents"],"award-info":[{"award-number":["X2024005-talents"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sulforaphane, a natural isothiocyanate predominantly found in cruciferous vegetables, has shown potential in preventing and treating Helicobacter pylori infection. However, the underlying metabolic mechanisms remain largely unclear. This study employed high-coverage metabolomics and lipidomics methods to comprehensively investigate the effects of sulforaphane on the serum and liver metabolic profiles of H. pylori-infected mice. Metabolomics and lipidomics analysis revealed that H. pylori infection disrupted multiple metabolic pathways, leading to perturbations in amino acids, fatty acids, bile acids, and various lipid species. Sulforaphane treatment can ameliorate these disruptions, notably reversing alterations in serum glycerophospholipids and restoring hepatic levels of amino acids, bile acids, glycerophospholipids, ceramides, and peptides. Key metabolic pathways implicated included glutathione metabolism and glycine and serine metabolism, which are associated with antioxidant defense and host resistance to pathogenic infections. These findings offer a comprehensive metabolic basis for understanding the therapeutic effects of sulforaphane against H. pylori infection.<\/jats:p>","DOI":"10.3390\/ijms26167791","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T07:45:31Z","timestamp":1755071131000},"page":"7791","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Therapeutic Effects of Sulforaphane on Helicobacter pylori-Infected Mice: Insights from High-Coverage Metabolomics and Lipidomics Analyses of Serum and Liver"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0173-2539","authenticated-orcid":false,"given":"Shuling","family":"He","sequence":"first","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9148-5369","authenticated-orcid":false,"given":"Lvyun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyao","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Disheng","family":"Feng","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Chinese Materia Medica, Institute of Structural Pharmacology & TCM Chemical Biology, College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou 350122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ansari, S., and Yamaoka, Y. (2019). Helicobacter pylori virulence factors exploiting gastric colonization and its pathogenicity. Toxins, 11.","DOI":"10.3390\/toxins11110677"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1158\/1055-9965.EPI-13-1057","article-title":"Gastric cancer: Descriptive epidemiology, risk factors, screening, and prevention","volume":"23","author":"Karimi","year":"2014","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"5578","DOI":"10.3748\/wjg.v25.i37.5578","article-title":"Pathogenesis and clinical management of Helicobacter pylori gastric infection","volume":"25","author":"Soares","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4066","DOI":"10.2174\/1381612823666170207103943","article-title":"Role of sulforaphane in protection of gastrointestinal tract against H. pylori and NSAID-induced oxidative stress","volume":"23","author":"Yanaka","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Xie, H., Chun, F.K.-H., Rutz, J., and Blaheta, R.A. (2021). Sulforaphane impact on reactive oxygen species (ROS) in bladder carcinoma. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115938"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Janczewski, \u0141. (2022). Sulforaphane and its bifunctional analogs: Synthesis and biological activity. Molecules, 27.","DOI":"10.3390\/molecules27051750"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"486","DOI":"10.5009\/gnl14040","article-title":"The effects of broccoli sprout extract containing sulforaphane on lipid peroxidation and Helicobacter pylori infection in the gastric mucosa","volume":"9","author":"Chang","year":"2015","journal-title":"Gut Liver"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"7610","DOI":"10.1073\/pnas.112203099","article-title":"Sulforaphane inhibits extracellular, intracellular, and antibiotic-resistant strains of Helicobacter pylori and prevents benzo[a]pyrene-induced stomach tumors","volume":"99","author":"Fahey","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.bbrc.2013.03.126","article-title":"Urease from Helicobacter pylori is inactivated by sulforaphane and other isothiocyanates","volume":"435","author":"Fahey","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1039\/B906712B","article-title":"Systems level studies of mammalian metabolomes: The roles of mass spectrometry and nuclear magnetic resonance spectroscopy","volume":"40","author":"Dunn","year":"2011","journal-title":"Chem. Soc. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhu, Y., Wang, P., Sha, W., and Sang, S. (2016). Urinary biomarkers of whole grain wheat intake identified by non-targeted and targeted metabolomics approaches. Sci. Rep., 6.","DOI":"10.1038\/srep36278"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.jgg.2019.11.009","article-title":"Integration of lipidomics and metabolomics for in-depth understanding of cellular mechanism and disease progression","volume":"47","author":"Wang","year":"2020","journal-title":"J. Genet. Genom."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.jcmgh.2017.02.002","article-title":"Oxidative stress resulting from Helicobacter pylori infection contributes to gastric carcinogenesis","volume":"3","author":"Butcher","year":"2017","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1038\/s41467-023-39487-1","article-title":"NOD1 mediates interleukin-18 processing in epithelial cells responding to Helicobacter pylori infection in mice","volume":"14","author":"Tran","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/j.1469-0691.2009.02719.x","article-title":"The pathological effect of Helicobacter pylori infection on liver tissues in mice","volume":"15","author":"Huang","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1716701","DOI":"10.1155\/2017\/1716701","article-title":"Multifarious beneficial effect of nonessential amino acid, glycine: A review","volume":"2017","author":"Razak","year":"2017","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1038\/s41467-019-11129-5","article-title":"Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing","volume":"10","author":"Cheng","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s00726-013-1493-1","article-title":"Glycine metabolism in animals and humans: Implications for nutrition and health","volume":"45","author":"Wang","year":"2013","journal-title":"Amino Acids"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6962","DOI":"10.1039\/D2FO00674J","article-title":"Cyclo(-Phe-Phe) alleviates chick embryo liver injury via activating the Nrf2 pathway","volume":"13","author":"Zhang","year":"2022","journal-title":"Food Funct."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zembron-Lacny, A., Wawrzyniak-Gramacka, E., Ksi\u0105\u017cek, A., Zagrodna, A., Kope\u0107, W., and S\u0142owi\u0144ska-Lisowska, M. (2022). Dipeptide extract modulates the oxi-antioxidant response to intense physical exercise. Nutrients, 14.","DOI":"10.3390\/nu14122402"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1080\/19490976.2019.1674124","article-title":"Diversification of host bile acids by members of the gut microbiota","volume":"11","author":"Winston","year":"2020","journal-title":"Gut Microbes"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Yang, M., Gu, Y., Li, L., Liu, T., Song, X., Sun, Y., Cao, X., Wang, B., Jiang, K., and Cao, H. (2021). Bile acid-gut microbiota axis in inflammatory bowel disease: From bench to Bedside. Nutrients, 13.","DOI":"10.3390\/nu13093143"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.1016\/j.cmet.2023.07.011","article-title":"Hyodeoxycholic acid alleviates non-alcoholic fatty liver disease through modulating the gut-liver axis","volume":"35","author":"Kuang","year":"2023","journal-title":"Cell Metab."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, H., Wang, Q., Chen, P., Zhou, C., Zhang, X., and Chen, L. (2021). Ursodeoxycholic acid treatment restores gut microbiota and alleviates liver inflammation in non-alcoholic steatohepatitic mouse model. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.788558"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Gao, J., Xu, K., Liu, H., Liu, G., Bai, M., Peng, C., Li, T., and Yin, Y. (2018). Impact of the gut microbiota on intestinal immunity mediated by tryptophan metabolism. Front. Cell. Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00013"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2400535","DOI":"10.1002\/mnfr.202400535","article-title":"Sulforaphane improves liver metabolism and gut microbiota in circadian rhythm disorder mice models fed with high-fat diets","volume":"68","author":"He","year":"2024","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.jpha.2019.11.007","article-title":"Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS","volume":"10","author":"Liu","year":"2020","journal-title":"J. Pharm. Anal."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s00432-024-05857-5","article-title":"Metabolomic profiling of upper GI malignancies in blood and tissue: A systematic review and meta-analysis","volume":"150","author":"Balonov","year":"2024","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10732748211041881","DOI":"10.1177\/10732748211041881","article-title":"Effect of Helicobacter pylori on plasma metabolic phenotype in patients with gastric cancer","volume":"28","author":"Wang","year":"2021","journal-title":"Cancer Control"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.addr.2020.06.028","article-title":"Bioactive lipids, inflammation and chronic diseases","volume":"159","author":"Leuti","year":"2020","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, Y., Xu, H., Zhao, Z., Dong, Y., Wang, X., and Niu, J. (2022). No evidence for a causal link between Helicobacter pylori infection and nonalcoholic fatty liver disease: A bidirectional mendelian randomization study. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.1018322"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6734809","DOI":"10.1155\/2018\/6734809","article-title":"Effect of Helicobacter pylori infection on serum lipid profile","volume":"2018","author":"Haeri","year":"2018","journal-title":"J. Lipids"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Xie, J., Wang, J., Zeng, R., and Xie, Y. (2023). Association between Helicobacter pylori infection and triglyceride levels: A nested cross-sectional study. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1220347"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1093\/ajcn\/74.6.737","article-title":"De novo lipogenesis during controlled overfeeding with sucrose or glucose in lean and obese women","volume":"74","author":"McDevitt","year":"2001","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Adeva-Andany, M.M., P\u00e9rez-Felpete, N., Fern\u00e1ndez-Fern\u00e1ndez, C., Donapetry-Garc\u00eda, C., and Pazos-Garc\u00eda, C. (2016). Liver glucose metabolism in humans. Biosci. Rep., 36.","DOI":"10.1042\/BSR20160385"},{"key":"ref_36","first-page":"1220","article-title":"Daily intake of sulforaphane-rich broccoli sprouts prevents progression of high salt diet-induced gastric atrophy in H. pylori-infected C57\/BL6 mice in vivo","volume":"65","author":"Yanaka","year":"2005","journal-title":"Cancer Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3171","DOI":"10.1007\/s11095-011-0500-z","article-title":"Metabolism and tissue distribution of sulforaphane in Nrf2 knockout and wild-type mice","volume":"28","author":"Clarke","year":"2011","journal-title":"Pharm. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.vph.2016.07.004","article-title":"Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways","volume":"85","author":"Holloway","year":"2016","journal-title":"Vasc. Pharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.taap.2017.04.010","article-title":"Increased seizure susceptibility and other toxicity symptoms following acute sulforaphane treatment in mice","volume":"326","author":"Nieoczym","year":"2017","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e2115753118","DOI":"10.1073\/pnas.2115753118","article-title":"Locust density shapes energy metabolism and oxidative stress resulting in divergence of flight traits","volume":"119","author":"Du","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W398","DOI":"10.1093\/nar\/gkae253","article-title":"MetaboAnalyst 6.0: Towards a unified platform for metabolomics data processing, analysis and interpretation","volume":"52","author":"Pang","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Li, J., Miao, B., Wang, S., Dong, W., Xu, H., Si, C., Wang, W., Duan, S., Lou, J., and Bao, Z. (2022). Hiplot: A comprehensive and easy-to-use web service for boosting publication-ready biomedical data visualization. Brief. Bioinform., 23.","DOI":"10.1093\/bib\/bbac261"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e85","DOI":"10.1002\/imt2.85","article-title":"OmicStudio: A composable bioinformatics cloud platform with real-time feedback that can generate high-quality graphs for publication","volume":"2","author":"Lyu","year":"2023","journal-title":"iMeta"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/16\/7791\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:25:49Z","timestamp":1760034349000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/16\/7791"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,12]]},"references-count":43,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["ijms26167791"],"URL":"https:\/\/doi.org\/10.3390\/ijms26167791","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,12]]}}}