{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:19:14Z","timestamp":1792016354720,"version":"4.4.0"},"reference-count":21,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:00:00Z","timestamp":1755216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health of the Russian Federation"},{"name":"LLC \u201cMonA.\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>\u03b2-adrenoreceptor (ADRB) ligands are actively used in the therapy of bronchopulmonary and cardiovascular diseases. When using these drugs, it is important to assess changes in ADRB content in different tissues. In most cases, the direct measurement of ADRB content in lung and heart cells is not possible. ADRB2 content in peripheral blood lymphocytes (or mononuclear cells) was shown to correlate with that in myocardial cells. It has been suggested that blood lymphocytes can be used to monitor ADRB content in solid organs. However, the estimation of ADRB1 content in myocardium from its content in peripheral lymphocytes is not possible due to the low content of ADRB1 in lymphocytes. In the present study, we performed simultaneous determination of ADRB1 and ADRB2 both in the total population of PBMCs and in isolated subpopulations of monocytes, T-lymphocytes, and NK-cells from 23 healthy donors using the modified radioligand method. The highest amount of ADRB2 was detected in NK cells, followed by PBMCs, monocytes, and T cells. The content of these receptors in all blood cell subpopulations was significantly correlated with each other, suggesting the possibility of using PBMCs to monitor ADRB2 in solid organs. For the first time, ADRB1 was detected in monocytes and NK cells.<\/jats:p>","DOI":"10.3390\/ijms26167894","type":"journal-article","created":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T16:09:55Z","timestamp":1755274195000},"page":"7894","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Novel Study of \u03b21- and \u03b22-Adrenergic Receptors Present on PBMCs, T Cells, Monocytes, and NK Cells by Radioligand Method: Quantitation and Correlations"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5485-4683","authenticated-orcid":false,"given":"Mihail. M.","family":"Peklo","sequence":"first","affiliation":[{"name":"National Medical Research Center of Cardiology Named After Academician E. I. Chazov, 121552 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina V.","family":"Smolyakova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Educational Institution of Higher Education \u201cRussian University of Medicine\u201d of the Ministry of Healthcare of the Russian Federation, 127006 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0176-5147","authenticated-orcid":false,"given":"Lyudmila N.","family":"Lipatova","sequence":"additional","affiliation":[{"name":"National Medical Research Center of Cardiology Named After Academician E. I. Chazov, 121552 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natal\u2019ya M.","family":"Kashirina","sequence":"additional","affiliation":[{"name":"National Medical Research Center of Cardiology Named After Academician E. I. Chazov, 121552 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurij S.","family":"Skoblov","sequence":"additional","affiliation":[{"name":"Institute of Bioorganic Chemistry Named After Academicians M. M. Shemyakin and Y. A. Ovchinnikov, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natal\u2019ya A.","family":"Skoblova","sequence":"additional","affiliation":[{"name":"Institute of Bioorganic Chemistry Named After Academicians M. M. Shemyakin and Y. A. Ovchinnikov, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihail A.","family":"Slinkin","sequence":"additional","affiliation":[{"name":"National Medical Research Center of Cardiology Named After Academician E. I. 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