{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:38:57Z","timestamp":1760060337701,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brain tumors are a significant medical problem. This study aimed to assess the potential significance of DPYSL2, 8-OHdG, and LP-PLA2 in the oncological diagnosis of patients with brain tumors who have a vitamin D deficiency. To this end, we measured the concentrations of LP-PLA2, DPYSL2, and 8-OHdG in 62 patients with different types of brain tumors who exhibited vitamin D deficiency. No significant variations were observed that would enable the differentiation of different types of intracranial tumors. However, we found a correlation between the concentrations of LP-PLA2 and DPYSL2 and tumor size (in the GBM and brain metastases groups). Additionally, we found a correlation between Lp-PLA2 and DPYSL2 levels in patients with GBM and brain metastases. In the control group, we identified correlations between 8-OHdG and Lp-PLA2, as well as between vitamin D levels and 8-OHdG. Further research is needed to fully comprehend the examined issues.<\/jats:p>","DOI":"10.3390\/ijms26167990","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T11:41:41Z","timestamp":1755603701000},"page":"7990","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of the Significance of LP-PLA2, DPYSL2, and 8-OHdG in the Oncological Diagnosis of Patients with Brain Tumors and Vitamin D Deficiency"],"prefix":"10.3390","volume":"26","author":[{"given":"Bart\u0142omiej","family":"Gromadzki","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-090 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Wici\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-090 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zygmunt","family":"Siedlecki","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Neurotraumatology and Pediatric Neurosurgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-094 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0141ukasz","family":"Rzepi\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Neurology, The 10th Military Research Hospital and Polyclinic, 85-681 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Fajkiel-Madajczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toru\u0144, 85-090 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"vi1","DOI":"10.1093\/neuonc\/noae145","article-title":"CBTRUS Statistical Report: Primary Brain and Other Central Nervous System Tumors Diagnosed in the United States in 2017\u20132021","volume":"26","author":"Price","year":"2024","journal-title":"Neuro. Oncol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2865","DOI":"10.1200\/JCO.2004.12.149","article-title":"Incidence proportions of brain metastases in patients diagnosed (1973 to 2001) in the Metropolitan Detroit Cancer Surveillance System","volume":"22","author":"Sloan","year":"2004","journal-title":"J. Clin. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.nec.2010.08.007","article-title":"Epidemiology of metastatic brain tumors","volume":"22","author":"Fox","year":"2011","journal-title":"Neurosurg. Clin. N. Am."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"P\u0142udowski, P., Kos-Kud\u0142a, B., Walczak, M., Fal, A., Zozuli\u0144ska-Zi\u00f3\u0142kiewicz, D., Sieroszewski, P., Peregud-Pogorzelski, J., Lauterbach, R., Targowski, T., and Lewi\u0144ski, A. (2023). Guidelines for Preventing and Treating Vitamin D Deficiency: A 2023 Update in Poland. Nutrients, 15.","DOI":"10.3390\/nu15030695"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Charoenngam, N., and Holick, M.F. (2020). Immunologic Effects of Vitamin D on Human Health and Disease. Nutrients, 12.","DOI":"10.3390\/nu12072097"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8428","DOI":"10.1080\/10408398.2022.2056574","article-title":"Association between vitamin D supplementation and cancer incidence and mortality: A trial sequential meta-analysis of randomized controlled trials","volume":"63","author":"Guo","year":"2023","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Abu El Maaty, M.A., and W\u00f6lfl, S. (2017). Vitamin D as a Novel Regulator of Tumor Metabolism: Insights on Potential Mechanisms and Implications for Anti-Cancer Therapy. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18102184"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/s41374-021-00673-8","article-title":"Anti-tumor effects of vitamin D in glioblastoma: Mechanism and therapeutic implications","volume":"102","author":"Lo","year":"2022","journal-title":"Lab. Investig."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1007\/s11011-019-00412-5","article-title":"From epidemiology and neurometabolism to treatment: Vitamin D in pathogenesis of glioblastoma Multiforme (GBM) and a proposal for Vitamin D + all-trans retinoic acid + Temozolomide combination in treatment of GBM","volume":"34","author":"Elmaci","year":"2019","journal-title":"Metab. Brain Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6256","DOI":"10.2174\/1381612820666140622200916","article-title":"Pathophysiological role and clinical significance of lipoprotein-associated phospholipase A2 (Lp-PLA2) bound to LDL and HDL","volume":"20","author":"Tellis","year":"2014","journal-title":"Curr. Pharm. Des."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kupert, E., Anderson, M., Liu, Y., Succop, P., Levin, L., Wang, J., Wikenheiser-brokamp, K., Chen, P., Pinney, S.M., and Macdonald, T. (2011). Plasma secretory phospholipase A2-IIa as a potential biomarker for lung cancer in patients with solitary pulmonary nodules. BMC Cancer, 11.","DOI":"10.1186\/1471-2407-11-513"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.canlet.2020.10.021","article-title":"Phospholipase A2 superfamily in cancer","volume":"497","author":"Peng","year":"2021","journal-title":"Cancer Lett."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.bcp.2007.04.021","article-title":"Phospholipase A2 as targets for anti-cancer drugs","volume":"74","author":"Cummings","year":"2007","journal-title":"Biochem. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Cai, H., Chiorean, E.G., Chiorean, M.V., Rex, D.K., Robb, B.W., Hahn, N.M., Liu, Z., Loehrer, P.J., Harrison, M.L., and Xu, Y. (2013). Elevated phospholipase A2 activities in plasma samples from multiple cancers. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057081"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"387","DOI":"10.4143\/crt.2016.106","article-title":"Underexpression of HOXA11 is associated with treatment resistance and poor prognosis in glioblastoma","volume":"49","author":"Se","year":"2017","journal-title":"Cancer Res. Treat."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Grant, N.J., Coates, P.J., Woods, Y.L., Bray, S.E., Morrice, N.A., Hastie, C.J., Lamont, D.J., Carey, F.A., and Sutherland, C. (2015). Phosphorylation of a splice variant of collapsin response mediator protein 2 in the nucleus of tumour cells links cyclin dependent kinase-5 to oncogenesis. BMC Cancer, 15.","DOI":"10.1186\/s12885-015-1691-1"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1038\/s41467-017-02811-7","article-title":"Tuning microtubule dynamics to enhance cancer therapy by modulating FER-mediated CRMP2 phosphorylation","volume":"9","author":"Zheng","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Abu Rmaileh, A., Solaimuthu, B., Khatib, A., Lavi, S., Tanna, M., Hayashi, A., Ben Yosef, M., Lichtenstein, M., Pillar, N., and Shaul, Y.D. (2022). DPYSL2 interacts with JAK1 to mediate breast cancer cell migration. J. Cell Biol., 221.","DOI":"10.1083\/jcb.202106078"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Qing, X., Shi, D., Lv, X., Wang, B., Chen, S., and Shao, Z. (2019). Prognostic significance of 8-hydroxy-2\u2032-deoxyguanosine in solid tumors: A meta-analysis. BMC Cancer, 19.","DOI":"10.1186\/s12885-019-6189-9"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1080S","DOI":"10.1093\/ajcn\/87.4.1080S","article-title":"Vitamin D deficiency: A worldwide problem with health consequences","volume":"87","author":"Holick","year":"2008","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1161\/ATVBAHA.112.249854","article-title":"Evidence supporting a key role of Lp-PLA2-generated lysophosphatidylcholine in human atherosclerotic plaque inflammation","volume":"32","author":"Edsfeldt","year":"2012","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1002\/biof.1890","article-title":"Oxidized phospholipids and lipoprotein- associated phospholipase A2 (Lp-PLA2) in atherosclerotic cardiovascular disease: An update","volume":"48","author":"Pantazi","year":"2022","journal-title":"Biofactors"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1093\/ije\/dyac087","article-title":"Vitamin D deficiency and C-reactive protein: A bidirectional Mendelian randomization study","volume":"52","author":"Zhou","year":"2023","journal-title":"Int. J. Epidemiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hibino, S., Kawazoe, T., Kasahara, H., Itoh, S., Ishimoto, T., Sakata-Yanagimoto, M., and Taniguchi, K. (2021). Inflammation-Induced Tumorigenesis and Metastasis. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115421"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1186\/1476-511X-10-170","article-title":"Antioxidant and inflammatory aspects of lipoprotein-associated phospholipase A2 (Lp-PLA2): A review","volume":"10","author":"Silva","year":"2011","journal-title":"Lipids Health Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1161\/ATVBAHA.115.305234","article-title":"Deciphering the Causal Role of sPLA2s and Lp-PLA2 in Coronary Heart Disease","volume":"35","author":"Talmud","year":"2015","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Borin, T.F., Angara, K., Rashid, M.H., Achyut, B.R., and Arbab, A.S. (2017). Arachidonic Acid Metabolite as a Novel Therapeutic Target in Breast Cancer Metastasis. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18122661"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Agulnik, D.M. (2012). Arachidonic acid metabolism and its implication on head and neck cancer. Head and Neck Cancer, InTech.","DOI":"10.5772\/1246"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1194\/jlr.R024190","article-title":"Modulation of oxidative stress, inflammation, and atherosclerosis by lipoprotein-associated phospholipase A2","volume":"53","author":"Rosenson","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1002\/cjp2.69","article-title":"PLA2G7 associates with hormone receptor negativity in clinical breast cancer samples and regulates epithelial-mesenchymal transition in cultured breast cancer cells","volume":"3","author":"Lehtinen","year":"2017","journal-title":"J. Pathol. Clin. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1111\/jcmm.17140","article-title":"High PLA2 level is correlated with glioblastoma progression via regulating DNA replication","volume":"26","author":"Zhang","year":"2022","journal-title":"J. Cell. Mol. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1158\/0008-5472.CAN-12-2374","article-title":"Deficiency of phospholipase A2 group 7 decreases intestinal polyposis and colon tumorigenesis in Apc(Min\/+) mice","volume":"73","author":"Xu","year":"2013","journal-title":"Cancer Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1002\/jcsm.12758","article-title":"Association of circulating PLA2G7 levels with cancer cachexia and assessment of darapladib as a therapy","volume":"12","author":"Morigny","year":"2021","journal-title":"J. Cachexia Sarcopenia Muscle"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Liao, Y., Badmann, S., Kraus, F., Topalov, N.E., Mayr, D., Kolben, T., Hester, A., Beyer, S., Mahner, S., and Jeschke, U. (2023). PLA2G7\/PAF-AH as Potential Negative Regulator of the Wnt Signaling Pathway Mediates Protective Effects in BRCA1 Mutant Breast Cancer. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010882"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e008094","DOI":"10.1136\/jitc-2023-008094","article-title":"Inhibiting PLA2G7 reverses the immunosuppressive function of intratumoral macrophages and augments immunotherapy response in hepatocellular carcinoma","volume":"12","author":"Zhang","year":"2024","journal-title":"J. Immunother. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1186\/s12957-021-02379-z","article-title":"DPYSL2 as potential diagnostic and prognostic biomarker linked to immune infiltration in lung adenocarcinoma","volume":"19","author":"Wu","year":"2021","journal-title":"World J. Surg. Oncol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1038\/s41419-023-06286-x","article-title":"Identification of multiple organ metastasis-associated hub mRNA\/miRNA signatures in non-small cell lung cancer","volume":"14","author":"Zu","year":"2023","journal-title":"Cell Death Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1002\/pmic.200700819","article-title":"Identification of collapsin response mediator protein-2 as a potential marker of colorectal carcinoma by comparative analysis of cancer cell secretomes","volume":"8","author":"Wu","year":"2008","journal-title":"Proteomics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/1758835920915302","article-title":"Anti-vimentin, anti-TUFM, anti-NAP1L1 and anti-DPYSL2 nanobodies display cytotoxic effect and reduce glioblastoma cell migration","volume":"12","author":"Zottel","year":"2020","journal-title":"Ther. Adv. Med. Oncol."},{"key":"ref_40","first-page":"2585","article-title":"Overview of the STAT-3 signaling pathway in cancer and the development of specific inhibitors","volume":"19","author":"Gu","year":"2020","journal-title":"Oncol. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Montero, P., Milara, J., Roger, I., and Cortijo, J. (2021). Role of JAK\/STAT in Interstitial Lung Diseases; Molecular and Cellular Mechanisms. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22126211"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1002\/med.21597","article-title":"Lipoprotein-associated phospholipase A2: The story continues","volume":"40","author":"Huang","year":"2020","journal-title":"Med. Res. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e15639","DOI":"10.7717\/peerj.15639","article-title":"Lp-PLA2 silencing ameliorates inflammation and autophagy in nonalcoholic steatohepatitis through inhibiting the JAK2\/STAT3 pathway","volume":"11","author":"Yao","year":"2023","journal-title":"PeerJ"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1038\/s41419-025-07593-1","article-title":"PLA2G7 promotes immune evasion of bladder cancer through the JAK-STAT-PDL1 axis","volume":"16","author":"Peng","year":"2025","journal-title":"Cell Death Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4103\/jcrt.JCRT_862_16","article-title":"Oxidative stress and its role in cancer","volume":"17","author":"Jelic","year":"2021","journal-title":"J. Cancer Res. Ther."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1097\/CAD.0000000000000602","article-title":"Anticancer effect of (S)-crizotinib on osteosarcoma cells by targeting MTH1 and activating reactive oxygen species","volume":"29","author":"Qing","year":"2018","journal-title":"Anti-Cancer Drugs"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.clon.2006.11.012","article-title":"Relationship between DNA damage and total antioxidant capacity in patients with glioblastoma multiforme","volume":"19","author":"Tuzgen","year":"2007","journal-title":"Clin. Oncol. (R. Coll. Radiol.)"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.mrfmmm.2012.02.005","article-title":"Does vitamin D protect against DNA damage?","volume":"733","author":"Armstrong","year":"2012","journal-title":"Mutat. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1158\/1055-9965.EPI-09-0448","article-title":"Effects of supplemental vitamin D and calcium on oxidative DNA damage marker in normal colorectal mucosa: A randomized clinical trial","volume":"19","author":"Fedirko","year":"2010","journal-title":"Cancer Epidemiol. Biomark. 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