{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:39:29Z","timestamp":1760060369521,"version":"build-2065373602"},"reference-count":75,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases","award":["DK 119482"],"award-info":[{"award-number":["DK 119482"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Distention of the urinary bladder wall during filling stretches the urothelium and induces the release of chemical mediators, including adenosine 5\u2032-triphosphate (ATP) and prostaglandins (PGs), that transmit signals between cells within the bladder wall. The urothelium also releases soluble nucleotidases (s-NTDs) that control the availability of ATP and its metabolites at receptor sites in umbrella cells and cells deeper in the bladder wall, as well as in the urine. This study investigated whether PGs regulate the intravesical breakdown of ATP by s-NTDs. Using a murine decentralized mucosa-only bladder model and an HPLC technology with fluorescence detection, we evaluated the decrease in ATP and increase in ADP, AMP, and adenosine (ADO) in intraluminal solutions (ILS) collected at the end of physiological bladder filling. PGD2, PGE2, and PGI2, but not PGF2\u03b1, inhibited the conversion of AMP (produced from ATP) to ADO, likely due to a suppressed intravesical release of s-AMPases. The effects of exogenous PGD2, PGE2, and PGI2 were mediated by DP1\/DP2, EP2, and IP prostanoid receptors, respectively. Activation of either DP1 or DP2 receptors by endogenous PGD2 also led to AMP increase and ADO decrease in ILS-containing ATP substrate. Finally, PGs produced by either COX-1 or COX-2 inhibited the hydrolysis of AMP to ADO. Together, these observations suggest that (1) endogenous PGs (chiefly PGD2, and to lesser degree PGE2 and PGI2) allow release of s-NTDs like s-ATPases and s-ADPases but impede the formation of ADO from intravesical ATP by inhibiting the release of s-NTDs\/s-AMPases; (2) it is possible that high concentrations of PGD2, PGE2 and PGI2, as anticipated in inflammation or bladder pain syndrome, delay the ADO production and prolong the action of excitatory purine mediators; and (3) either COX-1 and COX-2 are constitutively expressed in the mouse bladder mucosa or COX-2 is induced by distention of the urothelium during bladder filling.<\/jats:p>","DOI":"10.3390\/ijms26168023","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T15:29:29Z","timestamp":1755617369000},"page":"8023","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prostaglandins Regulate Urinary Purines by Modulating Soluble Nucleotidase Release in the Bladder Lumen"],"prefix":"10.3390","volume":"26","author":[{"given":"Mahsa","family":"Borhani Peikani","sequence":"first","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Gutierrez Cruz","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe S.","family":"Buckley","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2519-3323","authenticated-orcid":false,"given":"Violeta N.","family":"Mutafova-Yambolieva","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, Reno, NV 89557, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Durnin, L., Corrigan, R.D., Sanders, K.M., and Mutafova-Yambolieva, V.N. 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