{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:13:41Z","timestamp":1791940421016,"version":"4.3.4"},"reference-count":66,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["24-75-00027"],"award-info":[{"award-number":["24-75-00027"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Renocardiac syndrome type 4 (RCS4) is a common comorbid pathology, but the mechanisms of kidney dysfunction-induced cardiac remodeling and the involvement of cardiac progenitor cells (CPCs) in this process remain unclear. The aim of this study was to investigate the structural and functional changes in the cardiac muscle in RCS4 induced by unilateral ureteral obstruction (UUO) and the role of nestin+ CPCs in these. Heart function and localization of nestin+ cells in the myocardium were assessed using nestin-GFP transgenic mice subjected to UUO for 14 and 28 days. UUO resulted in cardiac hypertrophy, accompanied by an elongation of the QRS wave on the ECG, decreased expression of Cxcl1, Cxcl9, and Il1b, reduced the number of CD11b+ cells, and increased in titin isoform parameters, such as T1\/MHC and TT\/MHC ratios, without changes in fibrosis markers. The number of nestin+ cells increased in the myocardium with increased duration of UUO and displayed an SCA-1+TBX5+ phenotype, consistent with CPCs. Thus, cardiac pathology in RCS4 was manifested by cardiomyocyte hypertrophy with changes in the electrophysiological phenotype of the heart, not accompanied by fibrosis or inflammation. Nestin+ cardiac cells retained the CPC phenotype during UUO, and their number increased, which suggests their participation in regenerative processes in the heart.<\/jats:p>","DOI":"10.3390\/ijms26168100","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T07:41:45Z","timestamp":1755848505000},"page":"8100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Adaptive and Pathological Changes of the Cardiac Muscle in a Mouse Model of Renocardiac Syndrome: The Role of Nestin-Positive Cells"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7556-1617","authenticated-orcid":false,"given":"Polina A.","family":"Abramicheva","sequence":"first","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya A.","family":"Sokolov","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"School of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arina A.","family":"Druzhinina","sequence":"additional","affiliation":[{"name":"School of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0098-827X","authenticated-orcid":false,"given":"Daria M.","family":"Potashnikova","sequence":"additional","affiliation":[{"name":"School of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2720-4575","authenticated-orcid":false,"given":"Nadezda V.","family":"Andrianova","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9810-9391","authenticated-orcid":false,"given":"Dmitry S.","family":"Semenovich","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6404-8105","authenticated-orcid":false,"given":"Vasily N.","family":"Manskikh","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ljubava D.","family":"Zorova","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmira I.","family":"Yakupova","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Problems of the Russian Academy of Sciences, 123007 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6063-6789","authenticated-orcid":false,"given":"Ivan M.","family":"Vikhlyantsev","sequence":"additional","affiliation":[{"name":"Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Russia"},{"name":"Pushchino Branch of the Federal State Budgetary Educational Institution of Higher Education \u201cRussian Biotechnological University (BIOTECH University)\u201d, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4230-3849","authenticated-orcid":false,"given":"Olga S.","family":"Tarasova","sequence":"additional","affiliation":[{"name":"School of Biology, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"},{"name":"Department of Physiology and Pathology, Faculty of Basic Medicine, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0863-8490","authenticated-orcid":false,"given":"Dmitry B.","family":"Zorov","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2838-3704","authenticated-orcid":false,"given":"Egor Y.","family":"Plotnikov","sequence":"additional","affiliation":[{"name":"A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, 119992 Moscow, Russia"},{"name":"V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1159\/000350397","article-title":"Cardiorenal Syndrome Type 4: A Review","volume":"3","author":"Clementi","year":"2013","journal-title":"Cardiorenal Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/ckj\/sfac113","article-title":"From Cardiorenal Syndromes to Cardionephrology: A Reflection by Nephrologists on Renocardiac Syndromes","volume":"16","author":"Quiroga","year":"2022","journal-title":"Clin. Kidney J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s40697-015-0075-4","article-title":"Renocardiac Syndromes: Physiopathology and Treatment Stratagems","volume":"2","author":"Kingma","year":"2015","journal-title":"Can. J. Kidney Health Dis."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Buliga-Finis, O.N., Ouatu, A., Badescu, M.C., Dima, N., Tanase, D.M., Richter, P., and Rezus, C. (2022). Beyond the Cardiorenal Syndrome: Pathophysiological Approaches and Biomarkers for Renal and Cardiac Crosstalk. Diagnostics, 12.","DOI":"10.3390\/diagnostics12040773"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Klimova, E., Aparicio-Trejo, O.E., Tapia, E., and Pedraza-Chaverri, J. (2019). Unilateral Ureteral Obstruction as a Model to Investigate Fibrosis-Attenuating Treatments. Biomolecules, 9.","DOI":"10.3390\/biom9040141"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1161\/01.RES.83.1.1","article-title":"Ventricular Myocytes Are Not Terminally Differentiated in the Adult Mammalian Heart","volume":"83","author":"Anversa","year":"1998","journal-title":"Circ. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S0092-8674(03)00687-1","article-title":"Adult Cardiac Stem Cells Are Multipotent and Support Myocardial Regeneration","volume":"114","author":"Beltrami","year":"2003","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"127","DOI":"10.15171\/jcvtr.2017.22","article-title":"Cardiac Progenitor Cells Application in Cardiovascular Disease","volume":"9","author":"Amini","year":"2017","journal-title":"J. Cardiovasc. Thorac. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12551-014-0148-0","article-title":"Cardiac Stem Cells: Translation to Human Studies","volume":"7","author":"Ge","year":"2015","journal-title":"Biophys. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1016\/j.yjmcc.2008.07.013","article-title":"The Rat Heart Contains a Neural Stem Cell Population; Role in Sympathetic Sprouting and Angiogenesis","volume":"45","author":"Beguin","year":"2008","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/0092-8674(90)90662-X","article-title":"CNS Stem Cells Express a New Class of Intermediate Filament Protein","volume":"60","author":"Lendahl","year":"1990","journal-title":"Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1002\/jcp.22794","article-title":"Nestin Expression Is Lost in Ventricular Fibroblasts during Postnatal Development of the Rat Heart and Re-Expressed in Scar Myofibroblasts","volume":"227","author":"Gosselin","year":"2012","journal-title":"J. Cell. Physiol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Buyan, M.I., Andrianova, N.V., Popkov, V.A., Zorova, L.D., Pevzner, I.B., Silachev, D.N., Zorov, D.B., and Plotnikov, E.Y. (2022). Age-Associated Loss in Renal Nestin-Positive Progenitor Cells. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911015"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1089\/scd.2004.13.658","article-title":"Nestin Expression by Newly Formed Human Blood Vessels","volume":"13","author":"Filip","year":"2004","journal-title":"Stem Cells Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/jcp.20264","article-title":"Nestin-Expressing Neural Stem Cells Identified in the Scar Following Myocardial Infarction","volume":"204","author":"Drapeau","year":"2005","journal-title":"J. Cell. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1161\/01.HYP.0000187888.39665.d9","article-title":"Resident Nestin+ Neural-like Cells and Fibers Are Detected in Normal and Damaged Rat Myocardium","volume":"46","author":"Dupuis","year":"2005","journal-title":"Hypertension"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3389\/fcell.2018.00015","article-title":"The Biological Role of Nestin(+)-Cells in Physiological and Pathological Cardiovascular Remodeling","volume":"6","author":"Calderone","year":"2018","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_18","unstructured":"Vaidya, S.R., and Aeddula, N.R. (2024). Chronic Kidney Disease. StatPearls [Internet], StatPearls Publishing."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.ymeth.2025.02.007","article-title":"Cardiac Titin Isoforms: Practice in Interpreting Results of Electrophoretic Analysis","volume":"236","author":"Yakupova","year":"2025","journal-title":"Methods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"868466","DOI":"10.3389\/fcvm.2022.868466","article-title":"Cellular Heterogeneity of the Heart","volume":"9","author":"Michel","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"113231","DOI":"10.1016\/j.biopha.2022.113231","article-title":"Cardiorenal Dysfunction and Hypertrophy Induced by Renal Artery Occlusion Are Normalized by Galangin Treatment in Rats","volume":"152","author":"Chaihongsa","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_22","first-page":"744","article-title":"Cardiac Hypertrophy and Cardiac Cell Death in Chronic Kidney Disease","volume":"17","author":"Rofe","year":"2015","journal-title":"Isr. Med. Assoc. J. IMAJ"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s00395-024-01056-y","article-title":"Chronic Kidney Disease Activates the HDAC6-Inflammatory Axis in the Heart and Contributes to Myocardial Remodeling in Mice: Inhibition of HDAC6 Alleviates Chronic Kidney Disease-Induced Myocardial Remodeling","volume":"119","author":"Kundu","year":"2024","journal-title":"Basic. Res. Cardiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mishima, K., Nakasatomi, M., Takahashi, S., Ikeuchi, H., Sakairi, T., Kaneko, Y., Hiromura, K., Nojima, Y., and Maeshima, A. (2019). Attenuation of Renal Fibrosis after Unilateral Ureteral Obstruction in Mice Lacking the N-Type Calcium Channel. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223496"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"257","DOI":"10.2174\/1874192401004010257","article-title":"Experimental Models of Renal Disease and the Cardiovascular System","volume":"4","author":"Grossman","year":"2010","journal-title":"Open Cardiovasc. Med. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1161\/CIRCULATIONAHA.109.860171","article-title":"Cardiac Titin: A Multifunctional Giant","volume":"121","author":"LeWinter","year":"2010","journal-title":"Circulation"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.yjmcc.2014.10.020","article-title":"Increased Myocardial Stiffness due to Cardiac Titin Isoform Switching in a Mouse Model of Volume Overload Limits Eccentric Remodeling","volume":"79","author":"Hutchinson","year":"2015","journal-title":"J. Mol. Cell Cardiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"966968","DOI":"10.3389\/fcvm.2022.1016452","article-title":"Proteomic and Phosphoproteomic Profiling in Heart Failure with Preserved Ejection Fraction (HFpEF)","volume":"9","author":"Saw","year":"2022","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1161\/01.CIR.0000135591.37759.AF","article-title":"Altered Titin Expression, Myocardial Stiffness, and Left Ventricular Function in Patients with Dilated Cardiomyopathy","volume":"110","author":"Nagueh","year":"2004","journal-title":"Circulation"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.amjmed.2005.08.028","article-title":"Prognostic Significance of Quantitative QRS Duration","volume":"119","author":"Desai","year":"2006","journal-title":"Am. J. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1113\/jphysiol.2014.279380","article-title":"Misinterpretation of the Mouse ECG: \u201cMusing the Waves of Mus Musculus\u201d","volume":"592","author":"Boukens","year":"2014","journal-title":"J. Physiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1093\/cvr\/cvu047","article-title":"Interaction between Myofibroblasts and Stem Cells in the Fibrotic Heart: Balancing between Deterioration and Regeneration","volume":"102","author":"Ramkisoensing","year":"2014","journal-title":"Cardiovasc. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107673","DOI":"10.1016\/j.carpath.2024.107673","article-title":"Current Concepts in the Epigenetic Regulation of Cardiac Fibrosis","volume":"73","author":"Hassanabad","year":"2024","journal-title":"Cardiovasc. Pathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"52","DOI":"10.3389\/fcvm.2019.00052","article-title":"Anti-Fibrotic Effects of Cardiac Progenitor Cells in a 3D-Model of Human Cardiac Fibrosis","volume":"6","author":"Deddens","year":"2019","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1161\/CIRCGENETICS.115.001249","article-title":"CD36 Is a Matrix Metalloproteinase-9 Substrate That Stimulates Neutrophil Apoptosis and Removal During Cardiac Remodeling","volume":"9","author":"Tian","year":"2016","journal-title":"Circ. Cardiovasc. Genet."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"9446","DOI":"10.1038\/s41598-018-27857-5","article-title":"Relationship of Polymorphisms in the Tissue Inhibitor of Metalloproteinase (TIMP)-1 and -2 Genes with Chronic Heart Failure","volume":"8","author":"Polina","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1161\/HYPERTENSIONAHA.117.09045","article-title":"Tissue Inhibitor of Matrix Metalloproteinase-1 Promotes Myocardial Fibrosis by Mediating CD63\u2013Integrin \u03b21 Interaction","volume":"69","author":"Takawale","year":"2017","journal-title":"Hypertension"},{"key":"ref_38","first-page":"391","article-title":"Matrix Metalloproteinase-9: Many Shades of Function in Cardiovascular Disease","volume":"28","author":"Yabluchanskiy","year":"2013","journal-title":"Physiology (Bethesda)"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1016\/j.transproceed.2007.06.046","article-title":"Down-Regulation of Matrix Metalloproteinase-9 (MMP-9) Expression in the Myocardium of Congestive Heart Failure Patients","volume":"39","author":"Batlle","year":"2007","journal-title":"Transplant. Proc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s00395-008-0715-2","article-title":"Reduced MMP-2 Activity Contributes to Cardiac Fibrosis in Experimental Diabetic Cardiomyopathy","volume":"103","author":"Seeland","year":"2008","journal-title":"Basic. Res. Cardiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Tanase, D.M., Valasciuc, E., Anton, I.-B., Gosav, E.M., Dima, N., Cucu, A.I., Costea, C.F., Floria, D.E., Hurjui, L.L., and Tarniceriu, C.C. (2025). Matrix Metalloproteinases: Pathophysiologic Implications and Potential Therapeutic Targets in Cardiovascular Disease. Biomolecules, 15.","DOI":"10.3390\/biom15040598"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.pharmthera.2013.03.009","article-title":"Matrix Metalloproteinase (MMP)-9: A Proximal Biomarker for Cardiac Remodeling and a Distal Biomarker for Inflammation","volume":"139","author":"Halade","year":"2013","journal-title":"Pharmacol. Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"765768","DOI":"10.3389\/fphar.2021.765768","article-title":"The Role of CXC Chemokines in Cardiovascular Diseases","volume":"12","author":"Lu","year":"2021","journal-title":"Front. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"674498","DOI":"10.3389\/fcvm.2021.674498","article-title":"A Review of CXCL1 in Cardiac Fibrosis","volume":"8","author":"Wu","year":"2021","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.3389\/fimmu.2018.01932","article-title":"The Role of CXCR3 and Associated Chemokines in the Development of Atherosclerosis and during Myocardial Infarction","volume":"9","author":"Szentes","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e70040","DOI":"10.1002\/jcp.70040","article-title":"Nestin(+)- and Nestin(\u2212)-Ventricular Cardiomyocytes Reenter the Cell Cycle In Vitro but Are Reciprocally Regulated in the Partial Apex-Resected 7-Day Neonatal Rat Heart","volume":"240","author":"Aubry","year":"2025","journal-title":"J. Cell. Physiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Hertig, V., Tardif, K., Meus, M.A., Duquette, N., Villeneuve, L., Toussaint, F., Ledoux, J., and Calderone, A. (2017). Nestin Expression Is Upregulated in the Fibrotic Rat Heart and Is Localized in Collagen-Expressing Mesenchymal Cells and Interstitial CD31(+)- Cells. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0176147"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"848","DOI":"10.5966\/sctm.2012-0174","article-title":"Nestin Expression in End-Stage Disease in Dystrophin-Deficient Heart: Implications for Regeneration from Endogenous Cardiac Stem Cells","volume":"2","author":"Berry","year":"2013","journal-title":"Stem Cells Transl. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1161\/ATVBAHA.110.214726","article-title":"Neural Crest-Derived Stem Cells Migrate and Differentiate into Cardiomyocytes after Myocardial Infarction","volume":"31","author":"Tamura","year":"2011","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1083\/jcb.200504061","article-title":"Cardiac Neural Crest Cells Contribute to the Dormant Multipotent Stem Cell in the Mammalian Heart","volume":"170","author":"Tomita","year":"2005","journal-title":"J. Cell Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1002\/jcp.25496","article-title":"Nestin Expressed by Pre-Existing Cardiomyocytes Recapitulated in Part an Embryonic Phenotype; Suppressive Role of p38 MAPK","volume":"232","author":"Meus","year":"2017","journal-title":"J. Cell Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s00428-008-0631-8","article-title":"Re-Expression of Nestin in the Myocardium of Postinfarcted Patients","volume":"453","author":"Mokry","year":"2008","journal-title":"Virchows Arch."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.1089\/scd.2014.0197","article-title":"Sca-1+ Cardiac Progenitor Cells and Heart-Making: A Critical Synopsis","volume":"23","author":"Valente","year":"2014","journal-title":"Stem Cells Dev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1006\/dbio.2000.0134","article-title":"TBX5 Transcription Factor Regulates Cell Proliferation during Cardiogenesis","volume":"230","author":"Hatcher","year":"2001","journal-title":"Dev. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1242\/dev.126.8.1739","article-title":"Tbx5 Is Essential for Heart Development","volume":"126","author":"Horb","year":"1999","journal-title":"Development"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1053\/j.ackd.2018.08.004","article-title":"Cardiorenal Syndrome: An Overview","volume":"25","author":"Ronco","year":"2018","journal-title":"Adv. Chronic Kidney Dis."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1038\/s41371-023-00843-9","article-title":"Sex and Gender Differences in the Management of Chronic Kidney Disease and Hypertension","volume":"37","author":"Mayne","year":"2023","journal-title":"J. Hum. Hypertens."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"897","DOI":"10.21037\/atm.2020.03.62","article-title":"Understanding Sex Differences in Progression and Prognosis of Chronic Kidney Disease","volume":"8","author":"Kattah","year":"2020","journal-title":"Ann. Transl. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/cne.10964","article-title":"Neural Stem and Progenitor Cells in Nestin-GFP Transgenic Mice","volume":"469","author":"Mignone","year":"2004","journal-title":"J. Comp. Neurol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Mignone, J., Peunova, N., and Enikolopov, G. (2016). Nestin-Based Reporter Transgenic Mouse Lines. Multipotent Stem Cells of the Hair Follicle, Springer.","DOI":"10.1007\/978-1-4939-3786-8_2"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"054003","DOI":"10.1088\/1361-6579\/ab10d6","article-title":"Phase Synchronization of Baroreflex Oscillations of Blood Pressure and Pulse Interval in Rats: The Effects of Cardiac Autonomic Blockade and Gradual Blood Loss","volume":"40","author":"Negulyaev","year":"2019","journal-title":"Physiol. Meas."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s12551-017-0266-6","article-title":"Nuances of Electrophoresis Study of Titin\/connectin","volume":"9","author":"Vikhlyantsev","year":"2017","journal-title":"Biophys. Rev."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Bergmeyer, H.U. (1974). UV-Assay with Pyruvate and NADH. Methods of Enzymatic Analysis, Elsevier.","DOI":"10.1016\/B978-0-12-091302-2.50010-4"},{"key":"ref_64","first-page":"187","article-title":"Creatine Kinase Isoenzymes: Electrophoretic and Quantitative Measurements","volume":"15","author":"Foreback","year":"1981","journal-title":"Crit. Rev. Clin. Lab. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1783","DOI":"10.1007\/s00216-004-2525-1","article-title":"Determination of the Thiol Redox State of Organisms: New Oxidative Stress Indicators","volume":"378","author":"Patsoukis","year":"2004","journal-title":"Anal. Bioanal. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/16\/8100\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:33:06Z","timestamp":1760034786000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/16\/8100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,21]]},"references-count":66,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["ijms26168100"],"URL":"https:\/\/doi.org\/10.3390\/ijms26168100","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,21]]}}}