{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T20:21:11Z","timestamp":1760473271256,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interdisziplin\u00e4res Zentrum f\u00fcr Klinische Forschung W\u00fcrzburg","award":["A-371","AdvCSP-2","G-4960","G-5310"],"award-info":[{"award-number":["A-371","AdvCSP-2","G-4960","G-5310"]}]},{"name":"UAE University grants","award":["A-371","AdvCSP-2","G-4960","G-5310"],"award-info":[{"award-number":["A-371","AdvCSP-2","G-4960","G-5310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Allergic contact dermatitis (ACD) is a frequent inflammatory skin disease that evolves upon exposure to contact allergens in sensitized individuals. Both the adaptive and innate immune system play pivotal roles in the pathogenesis of ACD. While the importance of T cells is undisputed, the relevance of B lymphocytes is less clear. The published data support a critical role for NFATc1 in B cell activation. Therefore, we investigated the impact of NFATc1 on B cell function during murine contact hypersensitivity (CHS), the mouse model for human ACD. Compared with wild-type mice, B cell-specific ablation of NFATc1 (Nfatc1f\/f x mb1-cre) resulted in significantly diminished CHS responses measured by ear thickness (0.81 \u00b1 0.02 mm vs. 0.48 \u00b1 0.02 mm (p = 0.0007)) to the obligate contact allergen 2,4,6-trinitrochlorobenzene, accompanied by a marked increase in the frequency of IL-10-producing regulatory B cells. Flow cytometric analysis showed that IL-4- and IL-17-producing CD4+ T cells were reduced, while IFN-\u03b3-producing CD4+ T cells were marginally increased in Nfatc1f\/f x mb1-cre mice. In conclusion, NFATc1 mediates CHS responses by modulating the development of IL-10-producing B cells. These findings support the compelling notion that targeting NFATc1 may represent a potential therapeutic strategy for allergic responses.<\/jats:p>","DOI":"10.3390\/ijms26178125","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T12:31:26Z","timestamp":1755865886000},"page":"8125","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["NFATc1 Abrogation in B Cells Ameliorates Contact Hypersensitivity Responses"],"prefix":"10.3390","volume":"26","author":[{"given":"Franziska","family":"Gr\u00e4n","sequence":"first","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-1039","authenticated-orcid":false,"given":"Muhammad","family":"Azeem","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"},{"name":"Department of Molecular Pathology, Institute of Pathology, University of W\u00fcrzburg, 97078 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Serfling","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology, Institute of Pathology, University of W\u00fcrzburg, 97078 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7095-9848","authenticated-orcid":false,"given":"Matthias","family":"Goebeler","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6483-0191","authenticated-orcid":false,"given":"Andreas","family":"Kerstan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology and Allergology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6488-1722","authenticated-orcid":false,"given":"Khalid","family":"Muhammad","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, United Arab Emirates University, Al Ain 15551, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.mcna.2019.08.012","article-title":"Allergic contact dermatitis","volume":"104","author":"Nassau","year":"2020","journal-title":"Med. 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