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priority environmental pollutants because of their persistence, bioaccumulation, and endocrine-disrupting properties. As a characterized PAE-degrading strain of marine origin, Mycolicibacterium phocaicum RL-HY01 utilizes di-(2-ethylhexyl) phthalate (DEHP) as its sole carbon and energy source. Genome sequencing and RT-qPCR analysis revealed a previously uncharacterized hydrolase gene (dehpH) in strain RL-HY01, which catalyzes ester bond cleavage in PAEs. Subsequently, recombinant expression of the cloned dehpH gene from strain RL-HY01 was established in Escherichia coli BL21(DE3). The purified recombinant DehpH exhibited optimal activity at 30 \u00b0C and pH 8.0. Its activity was enhanced by Co2+ and tolerant to most metal ions but strongly inhibited by EDTA, SDS, and PMSF. Organic solvents (Tween-80, Triton X-100, methanol, ethanol, isopropanol, acetone, acetonitrile, ethyl acetate, and n-hexane) showed minimal impact. Substrate specificity assay indicated that DehpH could efficiently degrade the short and long side-chain PAEs but failed to hydrolyze the cyclic side-chain PAE (DCHP). The kinetics parameters for the hydrolysis of DEHP were determined under the optimized conditions, and DehpH had a Vmax of 0.047 \u00b1 0.002 \u03bcmol\/L\/min, Km of 462 \u00b1 50 \u03bcmol\/L, and kcat of 3.07 s\u22121. Computational prediction through structural modeling and docking identified the active site, with mutagenesis studies confirming Ser228, Asp324, and His354 as functionally indispensable residues forming the catalytic triad. The identification and characterization of DehpH provided novel insights into the mechanism of DEHP biodegradation and might promote the application of the target enzyme.<\/jats:p>","DOI":"10.3390\/ijms26178141","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T12:31:26Z","timestamp":1755865886000},"page":"8141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Identification and Characterization of a Novel Di-(2-ethylhexyl) Phthalate Hydrolase from a Marine Bacterial Strain Mycolicibacterium phocaicum RL-HY01"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3021-7246","authenticated-orcid":false,"given":"Lei","family":"Ren","sequence":"first","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caiyu","family":"Kuang","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongle","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1393-1608","authenticated-orcid":false,"given":"John L.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Faculty of Science and Engineering, University of Nottingham Ningbo China, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danting","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanqiao","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.envint.2016.09.013","article-title":"A global assessment of phthalates burden and related links to health effects","volume":"97","author":"Katsikantami","year":"2016","journal-title":"Environ. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s00253-017-8687-5","article-title":"Bacteria-mediated phthalic acid esters degradation and related molecular mechanisms","volume":"102","author":"Ren","year":"2018","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.2166\/wst.2023.291","article-title":"Uptake, accumulation, and degradation of dibutyl phthalate by three wetland plants","volume":"88","author":"Fan","year":"2023","journal-title":"Water Sci. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"137580","DOI":"10.1016\/j.jhazmat.2025.137580","article-title":"Uptake, translocation, and biotransformation of phthalate acid esters in crop plants: A comprehensive review","volume":"489","author":"Feng","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, J., Li, Y., Wang, Y., Li, Z., Li, X., Bao, H., Li, J., and Zhou, D. (2025). Transcriptome sequencing and metabolite analysis revealed the single and combined effects of microplastics and di-(2-ethylhexyl) phthalate on mouse liver. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26104943"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"143297","DOI":"10.1016\/j.chemosphere.2024.143297","article-title":"Antioxidants in mitigating phthalate-induced male reproductive toxicity: A comprehensive review","volume":"364","author":"Mondal","year":"2024","journal-title":"Chemosphere"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1007\/s12020-016-1158-4","article-title":"The influence of phthalates and bisphenol A on the obesity development and glucose metabolism disorders","volume":"55","author":"Stojanoska","year":"2017","journal-title":"Endocrine"},{"key":"ref_8","unstructured":"EPA, U. (2025, March 10). Code of Federal Regulations, 40 CFR, Part 1992, Available online: https:\/\/www.ecfr.gov\/current\/title-40\/chapter-I\/subchapter-N\/part-423\/appendix-Appendix%20A%20to%20Part%20423."},{"key":"ref_9","unstructured":"European, U. (2025, March 10). Council Regulation (EEC), No 793\/93 of 23 March 1993 on the Evaluation and Control of the Risks of Existing Substances (OJ L84, 5 April 1993). European Union, Brussels 1993. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=CELEX:31993R0793."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"139138","DOI":"10.1016\/j.scitotenv.2020.139138","article-title":"Bioremediation of di-(2-ethylhexyl) phthalate contaminated red soil by Gordonia terrae RL-JC02: Characterization, metabolic pathway and kinetics","volume":"733","author":"Zhang","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1126\/science.1236281","article-title":"Evaluating pesticide degradation in the environment: Blind spots and emerging opportunities","volume":"341","author":"Fenner","year":"2013","journal-title":"Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"143208","DOI":"10.1016\/j.scitotenv.2020.143208","article-title":"Complete biodegradation of di-n-butyl phthalate (DBP) by a novel Pseudomonas sp. YJB6","volume":"761","author":"Feng","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kamaraj, Y., Jayathandar, R.S., Dhayalan, S., Subramaniyan, S., and Punamalai, G. (2022). Biodegradation of di-(2-ethylhexyl) phthalate by novel Rhodococcus sp. PFS1 strain isolated from paddy field soil. Arch. Microbiol., 204.","DOI":"10.1007\/s00203-021-02632-9"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"16609","DOI":"10.1007\/s11356-016-6829-4","article-title":"Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples","volume":"23","author":"Ren","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/s00253-008-1632-x","article-title":"Biodegradation of dimethyl phthalate by Sphingomonas sp. isolated from phthalic-acid-degrading aerobic granules","volume":"80","author":"Zeng","year":"2008","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Durante Rodr\u00edguez, G., de Francisco Polanco, S., Fern\u00e1ndez Ar\u00e9valo, U., and D\u00edaz, E. (2024). Engineering bacterial biocatalysts for the degradation of phthalic acid esters. Microb. Biotechnol., 17.","DOI":"10.1111\/1751-7915.70024"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1038\/nrmicro2652","article-title":"Microbial degradation of aromatic compounds\u2014From one strategy to four","volume":"9","author":"Fuchs","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"148303","DOI":"10.1016\/j.scitotenv.2021.148303","article-title":"Phthalic acid esters degradation by a novel marine bacterial strain Mycolicibacterium phocaicum RL-HY01: Characterization, metabolic pathway and bioaugmentation","volume":"791","author":"Ren","year":"2021","journal-title":"Sci. Total Environ."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Gupta, R.S., Lo, B., and Son, J. (2018). Phylogenomics and comparative genomic studies robustly support division of the genus mycobacterium into an emended genus Mycobacterium and four novel genera. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00067"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12934-023-02096-0","article-title":"Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.","volume":"22","author":"Bhattacharyya","year":"2023","journal-title":"Microb. Cell. Fact."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sakdapetsiri, C., Jeerasantikul, C., Ningthoujam, R., Rungsihiranrut, A., and Pinyakong, O. (2025). Biodegradation pathways of phthalate esters by Mycolicibacterium parafortuitum J101 and its ability to enhance bioremediation. Int. Biodeterior. Biodegrad., 202.","DOI":"10.1016\/j.ibiod.2025.106085"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4113","DOI":"10.1007\/s00203-021-02398-0","article-title":"Identification and characterization of a novel carboxylesterase EstQ7 from a soil metagenomic library","volume":"203","author":"Yan","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6825","DOI":"10.1007\/s00253-019-09956-z","article-title":"Identification and characterization of a meta-cleavage product hydrolase involved in biphenyl degradation from Arthrobacter sp. YC-RL1","volume":"103","author":"Jia","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s10529-019-02693-3","article-title":"Molecular cloning, expression and characterization of a novel feruloyl esterase from a soil metagenomic library with phthalate-degrading activity","volume":"41","author":"Wu","year":"2019","journal-title":"Biotechnol. Lett."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"138393","DOI":"10.1016\/j.chemosphere.2023.138393","article-title":"Cloning and rational modification of a cold-adapted esterase for phthalate esters and parabens degradation","volume":"325","author":"Liu","year":"2023","journal-title":"Chemosphere"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1042\/bj3430177","article-title":"Bacterial lipolytic enzymes: Classification and properties","volume":"343","author":"Arpigny","year":"1999","journal-title":"Biochem. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.ijbiomac.2020.07.317","article-title":"Characterization of XtjR8: A novel esterase with phthalate-hydrolyzing activity from a metagenomic library of lotus pond sludge","volume":"164","author":"Qiu","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6870","DOI":"10.1128\/AEM.02072-14","article-title":"Newly identified thermostable esterase from Sulfobacillus acidophilus: Properties and performance in phthalate ester degradation","volume":"80","author":"Zhang","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S337","DOI":"10.1016\/j.molcatb.2017.02.004","article-title":"Gene cloning and characterization of a psychrophilic phthalate esterase with organic solvent tolerance from an Arctic bacterium Sphingomonas glacialis PAMC 26605","volume":"133","author":"Hong","year":"2016","journal-title":"J. Mol. Catal. B Enzym."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Jiao, Y., Chen, X., Wang, X., Liao, X., Xiao, L., Miao, A., Wu, J., and Yang, L. (2013). Identification and characterization of a cold-active phthalate esters hydrolase by screening a metagenomic library derived from biofilms of a wastewater treatment plant. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0075977"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.1007\/s00253-012-4232-8","article-title":"Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli","volume":"97","author":"Wu","year":"2013","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Wang, Y., Deng, C., and Wang, X. (2025). Characterization of a novel salt- and solvent-tolerant esterase Dhs82 from soil metagenome capable of hydrolyzing estrogenic phthalate esters. Biophys. Chem., 316.","DOI":"10.1016\/j.bpc.2024.107348"},{"key":"ref_33","first-page":"e202400014","article-title":"Metal Ion-mediated optical biosensors with signal amplification: Recent advances","volume":"4","author":"Jiang","year":"2024","journal-title":"Anal. Sens."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3687","DOI":"10.1016\/j.biortech.2010.11.072","article-title":"Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant","volume":"102","author":"Gopinath","year":"2011","journal-title":"Bioresour. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"118069","DOI":"10.1016\/j.envres.2023.118069","article-title":"Effects of heavy metals and metalloids on the biodegradation of organic contaminants","volume":"246","author":"Feng","year":"2024","journal-title":"Environ. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4309","DOI":"10.1111\/1462-2920.15613","article-title":"Genetic mapping of highly versatile and solvent-tolerant Pseudomonas putida \u2009B6-2\u2009(ATCC BAA-2545) as a \u2018superstar\u2019 for mineralization of PAHs and dioxin-like compounds","volume":"23","author":"Wang","year":"2021","journal-title":"Environ. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1101\/gr.215087.116","article-title":"Canu: Scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation","volume":"27","author":"Koren","year":"2017","journal-title":"Genome Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Brettin, T., Davis, J.J., Disz, T., Edwards, R.A., Gerdes, S., Olsen, G.J., Olson, R., Overbeek, R., Parrello, B., and Pusch, G.D. (2015). RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci. Rep., 5.","DOI":"10.1038\/srep08365"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"D206","DOI":"10.1093\/nar\/gkt1226","article-title":"The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)","volume":"42","author":"Overbeek","year":"2013","journal-title":"Nucleic. Acids. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"W30","DOI":"10.1093\/nar\/gkx343","article-title":"IslandViewer 4: Expanded prediction of genomic islands for larger-scale datasets","volume":"45","author":"Bertelli","year":"2017","journal-title":"Nucleic. Acids. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W484","DOI":"10.1093\/nar\/gkad326","article-title":"Proksee: In-depth characterization and visualization of bacterial genomes","volume":"51","author":"Grant","year":"2023","journal-title":"Nucleic. Acids. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"W320","DOI":"10.1093\/nar\/gku316","article-title":"Deciphering key features in protein structures with the new ENDscript server","volume":"42","author":"Robert","year":"2014","journal-title":"Nucleic. Acids. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"117597","DOI":"10.1016\/j.envpol.2021.117597","article-title":"Heterologous expression of bacterial cytochrome P450 from Microbacterium keratanolyticum ZY and its application in dichloromethane dechlorination","volume":"287","author":"Hu","year":"2021","journal-title":"Environ. Pollut."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1128\/JB.183.12.3689-3703.2001","article-title":"Plasmid-encoded phthalate catabolic pathway in Arthrobacter keyseri 12B","volume":"183","author":"Eaton","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.ibiod.2014.12.006","article-title":"Two endocrine disrupting dibutyl phthalate degrading esterases and their compensatory gene expression in Sphingobium sp. SM42","volume":"99","author":"Whangsuk","year":"2015","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1128\/AEM.72.4.2394-2399.2006","article-title":"A mono-2-ethylhexyl phthalate hydrolase from a Gordonia sp. that is able to dissimilate di-2-ethylhexyl phthalate","volume":"72","author":"Nishioka","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1128\/AEM.02621-09","article-title":"Involvement of a novel ABC transporter and monoalkyl phthalate ester hydrolase in phthalate ester catabolism by Rhodococcus jostii RHA1","volume":"76","author":"Hara","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s00284-016-1159-9","article-title":"Degradation of di(2-ethylhexyl) phthalate by a novel Gordonia alkanivorans strain YC-RL2","volume":"74","author":"Nahurira","year":"2017","journal-title":"Curr. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.jbiosc.2016.01.008","article-title":"Re-characterization of mono-2-ethylhexyl phthalate hydrolase belonging to the serine hydrolase family","volume":"122","author":"Iwata","year":"2016","journal-title":"J. Biosci. Bioeng."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Ding, J., Wang, C., Xie, Z., Li, J., Yang, Y., Mu, Y., Tang, X., Xu, B., Zhou, J., and Huang, Z. (2015). Properties of a newly identified esterase from Bacillus sp. K91 and its novel function in diisobutyl phthalate degradation. PLoS ONE., 10.","DOI":"10.1371\/journal.pone.0119216"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"8548","DOI":"10.1021\/acs.jafc.9b02655","article-title":"Biodegradation of structurally diverse phthalate esters by a newly identified esterase with catalytic activity toward di(2-ethylhexyl) phthalate","volume":"67","author":"Huang","year":"2019","journal-title":"J. Agric. Food. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Mu, B., Sadowski, P., Te\u2019O, J., Patel, B., Pathiraja, N., and Dudley, K. (2024). Identification and characterization of moderately thermostable diisobutyl phthalate degrading esterase from a Great Artesian Basin Bacillus velezensis NP05. Biotechnol. Rep., 42.","DOI":"10.1016\/j.btre.2024.e00840"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"135191","DOI":"10.1016\/j.jhazmat.2024.135191","article-title":"Molecular insights into the catalytic mechanism of a phthalate ester hydrolase","volume":"476","author":"Wang","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.jbiosc.2019.11.002","article-title":"Characterization of a novel carboxylesterase from Bacillus velezensis SYBC H47 and its application in degradation of phthalate esters","volume":"129","author":"Huang","year":"2020","journal-title":"J. Biosci. Bioeng."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s12934-020-01336-x","article-title":"Characterization of a novel family VIII esterase EstM2 from soil metagenome capable of hydrolyzing estrogenic phthalates","volume":"19","author":"Sarkar","year":"2020","journal-title":"Microb. Cell Fact."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/17\/8141\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:33:45Z","timestamp":1760034825000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/17\/8141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,22]]},"references-count":57,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["ijms26178141"],"URL":"https:\/\/doi.org\/10.3390\/ijms26178141","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,22]]}}}