{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T19:25:36Z","timestamp":1778700336763,"version":"3.51.4"},"reference-count":44,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Klebsiella pneumoniae, due to its capacity to produce numerous virulence factors and form biofilms, is one of the most significant etiological agents of nosocomial infections. The extensive and often unwarranted use of antibiotic therapy has driven the emergence of various mutations, adaptive mechanisms, and horizontal gene transfer among K. pneumoniae strains, resulting in resistance to most beta-lactam antibiotics, carbapenems, and the last-resort drug\u2014colistin. A promising alternative or adjunctive treatment is the application of rotating magnetic fields (RMFs). The present study aimed to evaluate changes in colistin susceptibility among 20 extended-spectrum beta-lactamases (ESBLs) and 20 K. pneumoniae carbapenemase (KPC)-positive K. pneumoniae strains isolated from hospital infections following exposure to RMF at frequencies of 5 and 50 Hz. Exposure to RMF at 5 Hz resulted in decreased colistin minimum inhibitory concentration (MIC) values in over half of the tested (ESBLs) and (KPC)-positive strains. Additionally, RMF at 50 Hz reduced colistin MIC values in 30% of (ESBL)-positive and 40% of (KPC)-positive strains. Therefore, in the future, RMF may be developed as a supportive therapeutic strategy to improve the efficacy of antibiotics in the treatment of infections caused by multidrug-resistant (MDR) pathogens, including colistin-resistant K. pneumoniae.<\/jats:p>","DOI":"10.3390\/ijms26178281","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T14:43:18Z","timestamp":1756219398000},"page":"8281","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of Colistin Susceptibility of Klebsiella pneumoniae Strains Exposed to Rotating Magnetic Field"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5785-9612","authenticated-orcid":false,"given":"Agata","family":"Pruss","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dagmara","family":"Kobyli\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Pomeranian Medical University in Szczecin, Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4915-6298","authenticated-orcid":false,"given":"Karol","family":"Fija\u0142kowski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Biotechnology, West Pomeranian University of Technology in Szczecin, Piastow 45, 70-311 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Masiuk","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Pomeranian Medical University in Szczecin, Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0016-0541","authenticated-orcid":false,"given":"Pawe\u0142","family":"Kwiatkowski","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Immunology, Pomeranian Medical University in Szczecin, Powstancow Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1097\/INF.0000000000001675","article-title":"Klebsiella pneumoniae: Virulence, biofilm and antimicrobial resistance","volume":"36","author":"Piperaki","year":"2017","journal-title":"Pediatr. 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