{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T03:41:02Z","timestamp":1790134862284,"version":"4.0.1"},"reference-count":57,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the FEDER\/Junta de Andaluc\u00eda-Consejer\u00eda de Universidad, Investigaci\u00f3n e Innovaci\u00f3n","award":["B-CTS-134-UGR20"],"award-info":[{"award-number":["B-CTS-134-UGR20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic wounds are characterized by persistent inflammation and microbial colonization, which interfere with the healing process and represent a significant clinical challenge. This study evaluated the anti-inflammatory and reparative potential of ellagic acid and punicalagin, along with their antimicrobial activity. Human dermal fibroblasts were exposed to a simulated inflammatory microenvironment induced by interleukin-1\u03b2 (IL-1\u03b2), Interleukin-6 (IL-6), and Tumor Necrosis Factor-\u03b1 (TNF) or bacterial lipopolysaccharides (LPS) and subsequently treated with ellagic acid or punicalagin (10\u22126 M and 10\u22127 M). Cell proliferation was assessed via MTT assay, and migration was evaluated using the scratch wound assay. IL-1\u03b2 and IL-6 secretion was quantified by Enzyme-Linked Immunosorbent Assay in LPS-treated fibroblasts. Antimicrobial activity against Candida albicans, Staphylococcus aureus, and Pseudomonas aeruginosa was analyzed using the disk diffusion method. Both compounds significantly enhanced fibroblast viability and migration under inflammation and reduced the secretion of IL-1\u03b2 and IL-6. However, no antimicrobial activity was observed at the tested concentrations. These findings suggest that ellagic acid and punicalagin may promote wound healing by modulating inflammation and supporting fibroblast function, despite lacking direct antimicrobial effect. Further in vivo studies are needed to validate their therapeutic relevance and explore their potential in the development of novel treatments for chronic wounds.<\/jats:p>","DOI":"10.3390\/ijms26178681","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T14:53:01Z","timestamp":1757083981000},"page":"8681","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Anti-Inflammatory and Antimicrobial Effect of Ellagic Acid and Punicalagin in Dermal Fibroblasts"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6631-7866","authenticated-orcid":false,"given":"Javier","family":"Ramos-Torrecillas","sequence":"first","affiliation":[{"name":"Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustraci\u00f3n 60, 18016 Granada, Spain"},{"name":"Institute of Biosanitary Research, ibs.Granada, C\/Doctor Azpitarte 4, 18012 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8132-305X","authenticated-orcid":false,"given":"Anabel","family":"Gonz\u00e1lez-Acedo","sequence":"additional","affiliation":[{"name":"Institute of Biosanitary Research, ibs.Granada, C\/Doctor Azpitarte 4, 18012 Granada, Spain"},{"name":"Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences of Melilla, University of Granada, C\/Santander 1, 52005 Melilla, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9176-6997","authenticated-orcid":false,"given":"Luc\u00eda","family":"Melguizo-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustraci\u00f3n 60, 18016 Granada, Spain"},{"name":"Institute of Biosanitary Research, ibs.Granada, C\/Doctor Azpitarte 4, 18012 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4332-6812","authenticated-orcid":false,"given":"Concepci\u00f3n","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. Ilustraci\u00f3n 60, 18016 Granada, Spain"},{"name":"Institute of Biosanitary Research, ibs.Granada, C\/Doctor Azpitarte 4, 18012 Granada, Spain"},{"name":"Institute of Neuroscience, Centro de Investigaci\u00f3n Biom\u00e9dica (CIBM), University of Granada, Parque de Tecnol\u00f3gico de la Salud (PTS), Avda. del Conocimiento S\/N, Armilla, 18016 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4381-5942","authenticated-orcid":false,"given":"Elvira","family":"De Luna-Bertos","sequence":"additional","affiliation":[{"name":"Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Avda. 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Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ogawa, R. (2017). Keloid and Hypertrophic Scars Are the Result of Chronic Inflammation in the Reticular Dermis. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18030606"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhao, R., Liang, H., Clarke, E., Jackson, C., and Xue, M. (2016). Inflammation in Chronic Wounds. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122085"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/apm.12099","article-title":"The Role of Bacterial Biofilms in Chronic Infections","volume":"121","author":"Bjarnsholt","year":"2013","journal-title":"APMIS Suppl."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1128\/CMR.14.2.244-269.2001","article-title":"Wound Microbiology and Associated Approaches to Wound Management","volume":"14","author":"Bowler","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kadam, S., Shai, S., Shahane, A., and Kaushik, K.S. (2019). Recent Advances in Non-Conventional Antimicrobial Approaches for Chronic Wound Biofilms: Have We Found the \u2018Chink in the Armor\u2019?. Biomedicines, 7.","DOI":"10.3390\/biomedicines7020035"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.124.4.401","article-title":"Fibroblasts, Myofibroblasts, and Wound Contraction","volume":"124","author":"Grinnell","year":"1994","journal-title":"J. Cell Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1038\/sj.jid.5700890","article-title":"TNF-Alpha Suppresses Alpha-Smooth Muscle Actin Expression in Human Dermal Fibroblasts: An Implication for Abnormal Wound Healing","volume":"127","author":"Goldberg","year":"2007","journal-title":"J. Investig. Dermatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nrm809","article-title":"Myofibroblasts and Mechano-Regulation of Connective Tissue Remodelling","volume":"3","author":"Tomasek","year":"2002","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.jbiomech.2009.09.020","article-title":"The Myofibroblast: Paradigm for a Mechanically Active Cell","volume":"43","author":"Hinz","year":"2010","journal-title":"J. Biomech."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e23","DOI":"10.1017\/S1462399411001943","article-title":"Inflammation and Wound Healing: The Role of the Macrophage","volume":"13","author":"Koh","year":"2011","journal-title":"Expert Rev. Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Celiksoy, V., Moses, R.L., Sloan, A.J., Moseley, R., and Heard, C.M. (2020). Evaluation of the In Vitro Oral Wound Healing Effects of Pomegranate (Punica Granatum) Rind Extract and Punicalagin, in Combination with Zn (II). Biomolecules, 10.","DOI":"10.3390\/biom10091234"},{"key":"ref_15","first-page":"8209","article-title":"Effect of Ellagic Acid on Damage Caused by Hepatic Ischemia Reperfusion in Rats","volume":"26","author":"Durgun","year":"2022","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Venusova, E., Kolesarova, A., Horky, P., and Slama, P. (2021). Physiological and Immune Functions of Punicalagin. Nutrients, 13.","DOI":"10.3390\/nu13072150"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.intimp.2014.02.004","article-title":"Ellagic Acid Protects against Carrageenan-Induced Acute Inflammation through Inhibition of Nuclear Factor Kappa B, Inducible Cyclooxygenase and Proinflammatory Cytokines and Enhancement of Interleukin-10 via an Antioxidant Mechanism","volume":"19","year":"2014","journal-title":"Int. Immunopharmacol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1039\/c2fo10258g","article-title":"Anti-Inflammatory Effects of Pomegranate (Punica granatum L.) Husk Ellagitannins in Caco-2 Cells, an in Vitro Model of Human Intestine","volume":"3","author":"Hollebeeck","year":"2012","journal-title":"Food Funct."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zeng, Y., Zhao, H., Zhang, T., Zhang, C., He, Y., Du, L., Zuo, F., and Wang, W. (2022). Lung-Protective Effect of Punicalagin on LPS-Induced Acute Lung Injury in Mice. Biosci. Rep., 42.","DOI":"10.1042\/BSR20212196"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.1093\/emboj\/18.17.4645","article-title":"Functional Overlap between Two Classes of Matrix-Degrading Proteases in Wound Healing","volume":"18","author":"Lund","year":"1999","journal-title":"EMBO J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.clindermatol.2006.12.005","article-title":"Impaired Wound Healing","volume":"25","author":"Menke","year":"2007","journal-title":"Clin. Dermatol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ruggeri, M., Bianchi, E., Rossi, S., Vigani, B., Bonferoni, M.C., Caramella, C., Sandri, G., and Ferrari, F. (2020). Nanotechnology-Based Medical Devices for the Treatment of Chronic Skin Lesions: From Research to the Clinic. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12090815"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Illescas-Montes, R., Rueda-Fern\u00e1ndez, M., Gonz\u00e1lez-Acedo, A., Melguizo-Rodr\u00edguez, L., Garc\u00eda-Recio, E., Ramos-Torrecillas, J., and Garc\u00eda-Mart\u00ednez, O. (2023). Effect of Punicalagin and Ellagic Acid on Human Fibroblasts In Vitro: A Preliminary Evaluation of Their Therapeutic Potential. Nutrients, 16.","DOI":"10.3390\/nu16010023"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Olchowik-Grabarek, E., Sekowski, S., Mierzwinska, I., Zukowska, I., Abdulladjanova, N., Shlyonsky, V., and Zamaraeva, M. (2024). Cell Type-Specific Anti- and Pro-Oxidative Effects of Punica granatum L. Ellagitannins. Membranes, 14.","DOI":"10.3390\/membranes14100218"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/tqem.22077","article-title":"Anticancer Activity of Polyphenolic Punica Granatum Peel Extracts Obtained by Hybrid Ultrasound-Microwave Assisted Extraction: Evaluation on HeLa and HepG2 Cells","volume":"33","year":"2024","journal-title":"Environ. Qual. Manag."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"15593258241264954","DOI":"10.1177\/15593258241264954","article-title":"Punicalagin Restricts Growth, Promotes Apoptosis, and Reduces Invasion in Human Gastric Cancer Cells","volume":"22","author":"Sun","year":"2024","journal-title":"Dose-Response Publ. Int. Hormesis Soc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.2174\/092986712800672049","article-title":"Intracellular Signaling Pathways Modulated by Phenolic Compounds: Application for New Anti-Inflammatory Drugs Discovery","volume":"19","author":"Costa","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ol\u0119dzka, A.J., and Czerwi\u0144ska, M.E. (2023). Role of Plant-Derived Compounds in the Molecular Pathways Related to Inflammation. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054666"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lawrence, T. (2009). The Nuclear Factor NF-kappaB Pathway in Inflammation. Cold Spring Harb. Perspect. Biol., 1.","DOI":"10.1101\/cshperspect.a001651"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4070","DOI":"10.1096\/fj.201701536R","article-title":"Endothelial microRNAs Regulating the NF-\u03baB Pathway and Cell Adhesion Molecules during Inflammation","volume":"32","author":"Zhong","year":"2018","journal-title":"FASEB J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"189","DOI":"10.4167\/jbv.2012.42.3.189","article-title":"Mitogen-Activated Protein Kinases in Inflammation","volume":"42","author":"Manzoor","year":"2012","journal-title":"J. Bacteriol. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1002\/bies.950180708","article-title":"Protein Kinase Cascades Activated by Stress and Inflammatory Cytokines","volume":"18","author":"Kyriakis","year":"1996","journal-title":"BioEssays"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1177\/154405910708600903","article-title":"P38 MAPK Signaling in Oral-Related Diseases","volume":"86","author":"Patil","year":"2007","journal-title":"J. Dent. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1080\/09540105.2017.1339670","article-title":"Ellagic Acid Protects against LPS-Induced Acute Lung Injury through Inhibition of Nuclear Factor Kappa B, Proinflammatory Cytokines and Enhancement of Interleukin-10","volume":"28","author":"Guan","year":"2017","journal-title":"Food Agric. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"BenSaad, L.A., Kim, K.H., Quah, C.C., Kim, W.R., and Shahimi, M. (2017). Anti-Inflammatory Potential of Ellagic Acid, Gallic Acid and Punicalagin A&B Isolated from Punica Granatum. BMC Complement. Altern. Med., 17.","DOI":"10.1186\/s12906-017-1555-0"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.intimp.2014.07.005","article-title":"Ellagic Acid Protects Lipopolysaccharide\/D-Galactosamine-Induced Acute Hepatic Injury in Mice","volume":"22","author":"Gu","year":"2014","journal-title":"Int. Immunopharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1177\/15353702231182230","article-title":"Oral Ellagic Acid Attenuated LPS-Induced Neuroinflammation in Rat Brain: MEK1 Interaction and M2 Microglial Polarization","volume":"248","author":"Liu","year":"2023","journal-title":"Exp. Biol. Med."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Cao, Y., Chen, J., Ren, G., Zhang, Y., Tan, X., and Yang, L. (2019). Punicalagin Prevents Inflammation in LPS- Induced RAW264.7 Macrophages by Inhibiting FoxO3a\/Autophagy Signaling Pathway. Nutrients, 11.","DOI":"10.3390\/nu11112794"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1002\/mnfr.201400163","article-title":"Punicalagin Inhibits Neuroinflammation in LPS-Activated Rat Primary Microglia","volume":"58","author":"Olajide","year":"2014","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.neuropharm.2017.01.025","article-title":"Inhibitory Effect of Punicalagin on Lipopolysaccharide-Induced Neuroinflammation, Oxidative Stress and Memory Impairment via Inhibition of Nuclear Factor-kappaB","volume":"117","author":"Kim","year":"2017","journal-title":"Neuropharmacology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1016\/j.jvs.2008.06.042","article-title":"The Relationship between Cytokine Concentrations and Wound Healing in Chronic Venous Ulceration","volume":"48","author":"Gohel","year":"2008","journal-title":"J. Vasc. Surg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/wrr.13210","article-title":"Cytokine Stability in Chronic Wound Fluid and Its Association to Fibroblast Proliferation","volume":"32","author":"Burian","year":"2024","journal-title":"Wound Repair Regen."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"153134","DOI":"10.1016\/j.phymed.2019.153134","article-title":"Phytochemicals Targeting Matrix Metalloproteinases Regulating Tissue Degradation in Inflammation and Rheumatoid Arthritis","volume":"66","author":"Alamgeer","year":"2020","journal-title":"Phytomedicine"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Acedo, A., Illescas-Montes, R., de Luna-Bertos, E., Ruiz, C., Ramos-Torrecillas, J., Garc\u00eda-Mart\u00ednez, O., and Melguizo-Rodr\u00edguez, L. (2024). Extra Virgin Olive Oil Phenolic Compounds Modulate the Gene Expression of Biomarkers Involved in Fibroblast Proliferation and Differentiation. Genes, 15.","DOI":"10.3390\/genes15020173"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zhang, X., Wang, S., Wu, Y., Liu, X., Wang, J., and Han, D. (2022). Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL\/6 Mice through Activating Nrf2 Mediated Signaling Pathway. Nutrients, 14.","DOI":"10.3390\/nu14051103"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Xiao, Y., Huang, R., Wang, N., Deng, Y., Tan, B., Yin, Y., Qi, M., and Wang, J. (2022). Ellagic Acid Alleviates Oxidative Stress by Mediating Nrf2 Signaling Pathways and Protects against Paraquat-Induced Intestinal Injury in Piglets. Antioxid. Basel Switz., 11.","DOI":"10.3390\/antiox11020252"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.jff.2019.02.015","article-title":"Pomegranate (Punica Granatum) Phenolics Ameliorate Hydrogen Peroxide-Induced Oxidative Stress and Cytotoxicity in Human Keratinocytes","volume":"54","author":"Liu","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Liu, H., Zhu, W., Zou, Y., and Xia, X. (2024). Antimicrobial Activity and Mechanisms of Punicalagin against Vibrio Parahaemolyticus. Foods, 13.","DOI":"10.3390\/foods13091366"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1089\/fpd.2016.2226","article-title":"Antimicrobial Activity of Punicalagin Against Staphylococcus Aureus and Its Effect on Biofilm Formation","volume":"14","author":"Xu","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mac\u00eado, N.S., Barbosa, C.R.D.S., Bezerra, A.H., Silveira, Z.d.S., da Silva, L., Coutinho, H.D.M., Dashti, S., Kim, B., da Cunha, F.A.B., and da Silva, M.V. (2022). Evaluation of Ellagic Acid and Gallic Acid as Efflux Pump Inhibitors in Strains of Staphylococcus Aureus. Biol. Open, 11.","DOI":"10.1242\/bio.059434"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1080\/09168451.2015.1116924","article-title":"Antimicrobial Activity of Hydroxytyrosol: A Current Controversy","volume":"80","author":"Truchado","year":"2016","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/2042-5783-2-5","article-title":"Growth Comparison of Several Escherichia Coli Strains Exposed to Various Concentrations of Lactoferrin Using Linear Spline Regression","volume":"2","author":"Sekse","year":"2012","journal-title":"Microb. Inform. Exp."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/j.1742-481X.2007.00347.x","article-title":"Interleukin-6 Concentrations in Wound Fluids Rather than Serological Markers Are Useful in Assessing Bacterial Triggers of Ulcer Inflammation","volume":"5","author":"Ambrosch","year":"2008","journal-title":"Int. Wound J."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"E238","DOI":"10.1002\/lary.27796","article-title":"Excessive Inflammation Portends Complications: Wound Cytokines and Head and Neck Surgery Outcomes","volume":"129","author":"Lassig","year":"2019","journal-title":"Laryngoscope"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.2337\/db12-1450","article-title":"Blocking Interleukin-1\u03b2 Induces a Healing-Associated Wound Macrophage Phenotype and Improves Healing in Type 2 Diabetes","volume":"62","author":"Mirza","year":"2013","journal-title":"Diabetes"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"D\u00edez-Tercero, L., Delgado, L.M., Bosch-Ru\u00e9, E., and Perez, R.A. (2021). Evaluation of the Immunomodulatory Effects of Cobalt, Copper and Magnesium Ions in a pro Inflammatory Environment. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-91070-0"},{"key":"ref_57","first-page":"56825","article-title":"A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration","volume":"133","author":"Cappiello","year":"2018","journal-title":"J. Vis. Exp. 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