{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T04:53:34Z","timestamp":1792040014423,"version":"4.4.0"},"reference-count":60,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T00:00:00Z","timestamp":1757462400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Science &amp; Technology Project of Anhui Province","award":["202423l10050003"],"award-info":[{"award-number":["202423l10050003"]}]},{"name":"Key Science &amp; Technology Project of Anhui Province","award":["JBGS [2021] 130"],"award-info":[{"award-number":["JBGS [2021] 130"]}]},{"name":"Key Science &amp; Technology Project of Anhui Province","award":["AB241484006"],"award-info":[{"award-number":["AB241484006"]}]},{"name":"Key Science &amp; Technology Project of Anhui Province","award":["2022-ZYXT-07"],"award-info":[{"award-number":["2022-ZYXT-07"]}]},{"name":"Project of Seed Industry Revitalization in Jiangsu Province, China","award":["202423l10050003"],"award-info":[{"award-number":["202423l10050003"]}]},{"name":"Project of Seed Industry Revitalization in Jiangsu Province, China","award":["JBGS [2021] 130"],"award-info":[{"award-number":["JBGS [2021] 130"]}]},{"name":"Project of Seed Industry Revitalization in Jiangsu Province, China","award":["AB241484006"],"award-info":[{"award-number":["AB241484006"]}]},{"name":"Project of Seed Industry Revitalization in Jiangsu Province, China","award":["2022-ZYXT-07"],"award-info":[{"award-number":["2022-ZYXT-07"]}]},{"name":"key research and development plan of Guangxi","award":["202423l10050003"],"award-info":[{"award-number":["202423l10050003"]}]},{"name":"key research and development plan of Guangxi","award":["JBGS [2021] 130"],"award-info":[{"award-number":["JBGS [2021] 130"]}]},{"name":"key research and development plan of Guangxi","award":["AB241484006"],"award-info":[{"award-number":["AB241484006"]}]},{"name":"key research and development plan of Guangxi","award":["2022-ZYXT-07"],"award-info":[{"award-number":["2022-ZYXT-07"]}]},{"name":"Major Technology Collaborative Promotion Plan for Largemouth Bass Industry in Jiangsu Province","award":["202423l10050003"],"award-info":[{"award-number":["202423l10050003"]}]},{"name":"Major Technology Collaborative Promotion Plan for Largemouth Bass Industry in Jiangsu Province","award":["JBGS [2021] 130"],"award-info":[{"award-number":["JBGS [2021] 130"]}]},{"name":"Major Technology Collaborative Promotion Plan for Largemouth Bass Industry in Jiangsu Province","award":["AB241484006"],"award-info":[{"award-number":["AB241484006"]}]},{"name":"Major Technology Collaborative Promotion Plan for Largemouth Bass Industry in Jiangsu Province","award":["2022-ZYXT-07"],"award-info":[{"award-number":["2022-ZYXT-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study presents the development of a quantitative evaluation method utilizing machine vision technology to characterize the extent of body surface damage in largemouth bass (Micropterus salmoides) infected with largemouth bass ranavirus (LMBV). High-resolution, multi-angle images (6000 \u00d7 4000 pixels) of the body surface from 239 infected specimens were acquired at a fixed distance of 40 cm using a SONY ILCE-7RM3 digital camera within a GODOX-LST60 softbox. Key parameters, including the number of segmented injury areas, the count of body surface lesions, and the total lesion area, were analyzed. These parameters were integrated through principal component analysis (PCA) to construct a comprehensive damage scoring model. The severity of viral-induced body surface damage was categorized into four grades: uninjured (0), minor injury (1), moderate injury (2), and severe injury (3). Histopathological examination revealed that early-stage infection (grade 1) predominantly exhibited localized hemorrhagic spots in the muscular region of the body side (B\/E region) with limited lesion area. In contrast, moderate to severe infections (grades 2\u20133) were characterized by extensive ulceration, muscle necrosis, and visceral lesions, including hepatic fibrosis and splenic granulomatous formations. Quantitative real-time PCR (qRT-PCR) analysis demonstrated a progressive upregulation of pro-inflammatory cytokines (IL-6, IL-8, TNF-\u03b1, CXCL2) in immune organs, concomitant with increased expression of apoptosis-related genes (CASP8, CYC). This study successfully established a rapid and objective quantitative grading system for ranavirus infection, offering a novel technical approach for early diagnosis and precise prevention and control strategies against largemouth bass ranavirus.<\/jats:p>","DOI":"10.3390\/ijms26188810","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T12:04:55Z","timestamp":1757505895000},"page":"8810","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of a Comprehensive Lesion Severity Classification Model for Largemouth Bass (Micropterus salmoides) Ranavirus (LMBV) Based on Machine Vision"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3465-2061","authenticated-orcid":false,"given":"Hui","family":"Sun","sequence":"first","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jixiang","family":"Hua","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-1775","authenticated-orcid":false,"given":"Yifan","family":"Tao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziying","family":"Yang","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taide","family":"Zhu","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqi","family":"Lu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0224-4511","authenticated-orcid":false,"given":"Wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalun","family":"Dong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linbing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jixiang","family":"He","sequence":"additional","affiliation":[{"name":"Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4718-6618","authenticated-orcid":false,"given":"Jun","family":"Qiang","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1080\/10641262.2012.753405","article-title":"Fish Matters: Importance of Aquatic Foods in Human Nutrition and Global Food Supply","volume":"21","author":"Tacon","year":"2013","journal-title":"Rev. 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