{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:21:33Z","timestamp":1768317693960,"version":"3.49.0"},"reference-count":23,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"START Project Promotion Type","award":["JPMJST1819"],"award-info":[{"award-number":["JPMJST1819"]}]},{"name":"START Project Promotion Type","award":["JP20K12649"],"award-info":[{"award-number":["JP20K12649"]}]},{"name":"START Project Promotion Type","award":["JP20K07156"],"award-info":[{"award-number":["JP20K07156"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JPMJST1819"],"award-info":[{"award-number":["JPMJST1819"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP20K12649"],"award-info":[{"award-number":["JP20K12649"]}]},{"name":"Japan Society for the Promotion of Science (JSPS) KAKENHI","award":["JP20K07156"],"award-info":[{"award-number":["JP20K07156"]}]},{"name":"Faculty of Pharmacy, Juntendo University","award":["JPMJST1819"],"award-info":[{"award-number":["JPMJST1819"]}]},{"name":"Faculty of Pharmacy, Juntendo University","award":["JP20K12649"],"award-info":[{"award-number":["JP20K12649"]}]},{"name":"Faculty of Pharmacy, Juntendo University","award":["JP20K07156"],"award-info":[{"award-number":["JP20K07156"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The study aim was to apply murine double minute 2 (MDM2)-siRNA to a biodegradable siRNA delivery vector, ternary complex, for treating colorectal cancer peritoneal dissemination. The ternary complex containing MDM2-siRNA (MDM2-siRNA complex) was constructed by mixing MDM2-siRNA, dendrigraft poly-L-lysine, and \u03b3-polyglutamic acid. Cellular uptake of the ternary complex and suppressive effect on MDM2-mRNA were determined in a mouse colorectal cancer cell line. Tumor-growth inhibition by the MDM2-siRNA complex was evaluated in peritoneal dissemination model mice. The MDM2-siRNA complex, with an approximately 177 nm particle size and \u221235 mV \u03b6-potential, prevented degradation of the inner siRNA by RNase. In the in vitro study, the ternary complex was highly taken up by the cells, and 2 \u03bcg\/mL of the MDM2-siRNA complex significantly decreased MDM2-mRNA to about 30% of control cells. Intraperitoneal administration in colorectal cancer peritoneal dissemination model mice showed little effect of the ternary complex containing scramble-siRNA on cancer growth in the peritoneal cavity. Conversely, the MDM2-siRNA complex significantly reduced peritoneal dissemination to less than 1\/1000th of control mice and successfully prolonged survival time. In this study, we found that the biodegradable MDM2-siRNA complex had a suppressive effect on MDM2-mRNA in cancer cells and tumor growth of peritoneal dissemination.<\/jats:p>","DOI":"10.3390\/ijms26188883","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T12:08:16Z","timestamp":1757678896000},"page":"8883","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biodegradable Nanoparticles Encapsulating Murine Double Minute 2 siRNA to Treat Peritoneal Dissemination of Colon Cancer"],"prefix":"10.3390","volume":"26","author":[{"given":"Tomoaki","family":"Kurosaki","sequence":"first","affiliation":[{"name":"Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"},{"name":"Faculty of Pharmacy, Juntendo University, 6-8-1, Hinode, Urayasu 279-0013, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akari","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuuki","family":"Takashima","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Juntendo University, 6-8-1, Hinode, Urayasu 279-0013, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukinobu","family":"Kodama","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: Sources, methods, and major patterns in GLOBOCAN 2012","volume":"136","author":"Ferlay","year":"2015","journal-title":"Int. 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