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007491"],"award-info":[{"award-number":["OCI-01-0145-FEDER- 007491"]}]},{"name":"COMPETE 2020","award":["UIDB\/00215\/2020"],"award-info":[{"award-number":["UIDB\/00215\/2020"]}]},{"name":"COMPETE 2020","award":["UIDP\/00215\/2020"],"award-info":[{"award-number":["UIDP\/00215\/2020"]}]},{"name":"COMPETE 2020","award":["LA\/P\/0064\/2020"],"award-info":[{"award-number":["LA\/P\/0064\/2020"]}]},{"name":"COMPETE 2020","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}]},{"name":"COMPETE 2020","award":["UI\/BD\/150750\/2020"],"award-info":[{"award-number":["UI\/BD\/150750\/2020"]}]},{"name":"COMPETE 2020","award":["OCI-01-0145-FEDER- 007491"],"award-info":[{"award-number":["OCI-01-0145-FEDER- 007491"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Male fertility has declined over the years, partly due to metabolic disorders such as obesity and Type 2 diabetes. Antidiabetic drugs, including GLP-1 receptor agonists like liraglutide, are widely used to manage these conditions and aid in weight loss. Within the male reproductive tract, Leydig cells (LCs) are essential since they produce testosterone. Notably, the influence of antidiabetics on LCs remains a subject of limited investigation. Herein, we aimed to evaluate the effect of liraglutide on the physiology of LCs. To this end, we cultured LCs (BLTK1 cell line) without (control) or in the presence of selected concentrations of liraglutide. We then assessed their metabolic viability, cell proliferation, LDH release, ROS production, mitochondrial membrane potential, and in vivo mitochondrial cell performance, as well as the number of mtDNA copies. We also measured androstenedione production. Our results showed that liraglutide at pharmacological and supra-pharmacological concentrations increased the metabolic viability of LCs and reduced ROS production at all concentrations. Furthermore, the pharmacological concentration of liraglutide increased the basal respiration, maximal respiration, proton leak, and oxygen consumption rate related to ATP-linked production. Androstenedione production remained unchanged, which may be related to the inherent limitations of the cell line in supporting steroidogenesis. Overall, our findings suggest that liraglutide exhibits a potential protective effect on LC function, particularly by enhancing metabolic viability, reducing oxidative stress, and improving mitochondrial performance, highlighting its potential beyond the established role in diabetes and weight management.<\/jats:p>","DOI":"10.3390\/ijms26188903","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T12:34:03Z","timestamp":1757680443000},"page":"8903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Pharmacological Dose of Liraglutide Improves Mitochondrial Performance in Mouse Leydig Cells"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5972-8096","authenticated-orcid":false,"given":"Bruno","family":"Oliveira-Lopes","sequence":"first","affiliation":[{"name":"Unit for Multidisciplinary Research in Biomedicine (UMIB), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, 4050-313 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3813-8107","authenticated-orcid":false,"given":"Patr\u00edcia C.","family":"Braga","sequence":"additional","affiliation":[{"name":"Unit for Multidisciplinary Research in Biomedicine (UMIB), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, 4050-313 Porto, Portugal"},{"name":"Laboratory for Integrative and Translational Research in Population Health (ITR), 4050-600 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4989-5699","authenticated-orcid":false,"given":"Pedro F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, 3010-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7635-783X","authenticated-orcid":false,"given":"Marco G.","family":"Alves","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel L.","family":"Bernardino","sequence":"additional","affiliation":[{"name":"Unit for Multidisciplinary Research in Biomedicine (UMIB), School of Medicine and Biomedical Sciences (ICBAS), University of Porto, 4050-313 Porto, Portugal"},{"name":"Laboratory for Integrative and Translational Research in Population Health (ITR), 4050-600 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1093\/humrep\/dem077","article-title":"Insulin dependant diabetes mellitus: Implications for male reproductive function","volume":"22","author":"Agbaje","year":"2007","journal-title":"Hum. Reprod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1542\/peds.2012-3769","article-title":"Annual summary of vital statistics: 2010\u20132011","volume":"131","author":"Hamilton","year":"2013","journal-title":"Pediatrics"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1038\/nrd3148","article-title":"Liraglutide","volume":"9","author":"Drucker","year":"2010","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Rato, L., Oliveira, P.F., Sousa, M., Silva, B.M., and Alves, M.G. (2019). Role of reactive oxygen species in diabetes-induced male reproductive dysfunction. Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction, Elsevier.","DOI":"10.1016\/B978-0-12-812501-4.00014-6"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.diabres.2011.12.015","article-title":"An overview of the pharmacokinetics, efficacy and safety of liraglutide","volume":"97","author":"Bode","year":"2012","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.2147\/DDDT.S58459","article-title":"Liraglutide and obesity: A review of the data so far","volume":"9","author":"Ladenheim","year":"2015","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"187","DOI":"10.2337\/db11-1029","article-title":"Na+-D-glucose cotransporter SGLT1 is pivotal for intestinal glucose absorption and glucose-dependent incretin secretion","volume":"61","author":"Gorboulev","year":"2012","journal-title":"Diabetes"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.peptides.2017.12.013","article-title":"Control of insulin secretion by GLP-1","volume":"100","author":"Jones","year":"2018","journal-title":"Peptides"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Chaudhury, A., Duvoor, C., Reddy Dendi, V.S., Kraleti, S., Chada, A., Ravilla, R., Marco, A., Shekhawat, N.S., Montales, M.T., and Kuriakose, K. (2017). Clinical Review of Antidiabetic Drugs: Implications for Type 2 Diabetes Mellitus Management. Front. Endocrinol., 8.","DOI":"10.3389\/fendo.2017.00006"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"392","DOI":"10.2174\/157339911797579160","article-title":"Pharmacological management of type 2 diabetes mellitus: An update","volume":"7","author":"Sherbeeni","year":"2011","journal-title":"Curr. Diabetes Rev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Pelusi, C. (2022). The Effects of the New Therapeutic Treatments for Diabetes Mellitus on the Male Reproductive Axis. Front. Endocrinol., 13.","DOI":"10.3389\/fendo.2022.821113"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1093\/biolre\/iox087","article-title":"GLP-1 increases Kiss-1 mRNA expression in kisspeptin-expressing neuronal cells","volume":"97","author":"Oride","year":"2017","journal-title":"Biol. Reprod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1935","DOI":"10.1111\/andr.12871","article-title":"Glucagon-like peptide-1 receptor is expressed in human and rodent testis","volume":"8","author":"Caltabiano","year":"2020","journal-title":"Andrology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1111\/andr.13015","article-title":"Is there a role for glucagon-like peptide-1 receptor agonists in the treatment of male infertility?","volume":"9","author":"Cannarella","year":"2021","journal-title":"Andrology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.taap.2018.10.009","article-title":"Metabolic dynamics of human Sertoli cells are differentially modulated by physiological and pharmacological concentrations of GLP-1","volume":"362","author":"Martins","year":"2019","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1210\/endo.141.2.7326","article-title":"Neuroendocrine function and response to stress in mice with complete disruption of glucagon-like peptide-1 receptor signaling","volume":"141","author":"MacLusky","year":"2000","journal-title":"Endocrinology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1111\/aji.12420","article-title":"GLP-1 Receptor Agonist Exenatide Attenuates the Detrimental Effects of Obesity on Inflammatory Profile in Testis and Sperm Quality in Mice","volume":"74","author":"Zhang","year":"2015","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1111\/andr.12099","article-title":"Adding liraglutide to lifestyle changes, metformin and testosterone therapy boosts erectile function in diabetic obese men with overt hypogonadism","volume":"3","author":"Giagulli","year":"2015","journal-title":"Andrology"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"La Vignera, S., Condorelli, R.A., Calogero, A.E., Cannarella, R., and Aversa, A. (2023). Sexual and Reproductive Outcomes in Obese Fertile Men with Functional Hypogonadism After Treatment with Liraglutide: Preliminary Results. J. Clin. Med., 12.","DOI":"10.3390\/jcm12020672"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.rbmo.2014.07.009","article-title":"The effects of liraglutide on male fertility: A case report","volume":"29","author":"Fontoura","year":"2014","journal-title":"Reprod. Biomed. Online"},{"key":"ref_21","first-page":"109","article-title":"Effects of liraglutide on sperm characteristics and fertilization potential following experimentally induced diabetes in mice","volume":"12","author":"Pourheydar","year":"2021","journal-title":"Vet. Res. Forum"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.cmet.2023.02.003","article-title":"The global burden of metabolic disease: Data from 2000 to 2019","volume":"35","author":"Chew","year":"2023","journal-title":"Cell Metab."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wronka, M., Krzemi\u0144ska, J., M\u0142ynarska, E., Rysz, J., and Franczyk, B. (2023). New Insights into the Use of Liraglutide\u2014Impact on Cardiovascular Risk and Microvascular Outcomes. Biomedicines, 11.","DOI":"10.3390\/biomedicines11041159"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.3390\/cimb45020092","article-title":"Urinary Proteome Differences in Patients with Type 2 Diabetes Pre and Post Liraglutide Treatment","volume":"45","author":"Rafiullah","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_25","first-page":"79","article-title":"Reproductive dysfunctions in males with type 2 diabetes mellitus: An updated review","volume":"8","author":"Ray","year":"2020","journal-title":"Diabetes"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1177\/0091270011430504","article-title":"Dosing rationale for liraglutide in type 2 diabetes mellitus: A pharmacometric assessment","volume":"52","author":"Ingwersen","year":"2012","journal-title":"J. Clin. Pharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1002\/phar.1639","article-title":"Liraglutide: A New Option for the Treatment of Obesity","volume":"35","author":"Nuffer","year":"2015","journal-title":"Pharmacotherapy"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Salvio, G., Ciarloni, A., Ambo, N., Bordoni, M., Perrone, M., Rossi, S., and Balercia, G. (2025). Effects of glucagon-like peptide 1 receptor agonists on testicular dysfunction: A systematic review and meta-analysis. Andrology.","DOI":"10.1111\/andr.70022"},{"key":"ref_29","first-page":"233","article-title":"The Akt\/FoxO1\/p27 pathway mediates the proliferative action of liraglutide in beta cells","volume":"5","author":"Fang","year":"2012","journal-title":"Mol. Med. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2366","DOI":"10.1159\/000430199","article-title":"The neuroprotective effect of liraglutide is mediated by glucagon-like peptide 1 receptor-mediated activation of cAMP\/PKA\/CREB pathway","volume":"36","author":"Bao","year":"2015","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zhang, L., Tian, J., Diao, S., Zhang, G., Xiao, M., and Chang, D. (2020). GLP-1 receptor agonist liraglutide protects cardiomyocytes from IL-1beta-induced metabolic disturbance and mitochondrial dysfunction. Chem. Biol. Interact., 332.","DOI":"10.1016\/j.cbi.2020.109252"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3924","DOI":"10.1210\/en.2005-1204","article-title":"Energized, polarized, and actively respiring mitochondria are required for acute Leydig cell steroidogenesis","volume":"147","author":"Allen","year":"2006","journal-title":"Endocrinology"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Ardalan, A., Smith, M.D., and Jelokhani-Niaraki, M. (2022). Uncoupling Proteins and Regulated Proton Leak in Mitochondria. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031528"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.freeradbiomed.2004.10.016","article-title":"Mitochondrial H+ leak and ROS generation: An odd couple","volume":"38","author":"Brookes","year":"2005","journal-title":"Free Radic. Biol. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1007\/978-3-319-55330-6_20","article-title":"Mitochondrial Proton Leak Plays a Critical Role in Pathogenesis of Cardiovascular Diseases","volume":"982","author":"Cheng","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.bbrc.2018.03.142","article-title":"Liraglutide attenuates NLRP3 inflammasome-dependent pyroptosis via regulating SIRT1\/NOX4\/ROS pathway in H9c2 cells","volume":"499","author":"Chen","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1288","DOI":"10.1016\/j.bcp.2013.02.015","article-title":"SIRT1 inhibits NADPH oxidase activation and protects endothelial function in the rat aorta: Implications for vascular aging","volume":"85","author":"Zarzuelo","year":"2013","journal-title":"Biochem. Pharmacol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1002\/jcb.21625","article-title":"Maintenance of mitochondrial DNA copy number and expression are essential for preservation of mitochondrial function and cell growth","volume":"103","author":"Jeng","year":"2008","journal-title":"J. Cell. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e00527","DOI":"10.1016\/j.heliyon.2018.e00527","article-title":"Currently available murine Leydig cell lines can be applied to study early steps of steroidogenesis but not testosterone synthesis","volume":"4","author":"Engeli","year":"2018","journal-title":"Heliyon"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1111\/bph.12313","article-title":"Molecular mechanisms underlying physiological and receptor pleiotropic effects mediated by GLP-1R activation","volume":"171","author":"Pabreja","year":"2014","journal-title":"Br. J. Pharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1210\/endrev\/bnaa032","article-title":"Revisiting the Complexity of GLP-1 Action from Sites of Synthesis to Receptor Activation","volume":"42","author":"McLean","year":"2021","journal-title":"Endocr. Rev."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Puddu, A., and Maggi, D. (2021). Emerging Role of Caveolin-1 in GLP-1 Action. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.668012"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/toxsci\/kfs121","article-title":"BLTK1 murine Leydig cells: A novel steroidogenic model for evaluating the effects of reproductive and developmental toxicants","volume":"127","author":"Forgacs","year":"2012","journal-title":"Toxicol. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s40262-015-0343-6","article-title":"Liraglutide in Type 2 Diabetes Mellitus: Clinical Pharmacokinetics and Pharmacodynamics","volume":"55","author":"Tichopad","year":"2016","journal-title":"Clin. Pharmacokinet."},{"key":"ref_45","first-page":"3520","article-title":"Liraglutide promotes apoptosis of HepG2 cells by activating JNK signaling pathway","volume":"23","author":"Li","year":"2019","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e85","DOI":"10.1002\/cptx.85","article-title":"Assessment of Sertoli Cell Proliferation by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide and Sulforhodamine B Assays","volume":"81","author":"Martins","year":"2019","journal-title":"Curr. Protoc. Toxicol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e122","DOI":"10.1093\/nar\/gng122","article-title":"Standardized determination of real-time PCR efficiency from a single reaction set-up","volume":"31","author":"Tichopad","year":"2003","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/18\/8903\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:44:36Z","timestamp":1760035476000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/18\/8903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,12]]},"references-count":47,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["ijms26188903"],"URL":"https:\/\/doi.org\/10.3390\/ijms26188903","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,12]]}}}