{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T04:26:37Z","timestamp":1772252797045,"version":"3.50.1"},"reference-count":72,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005191","name":"FIU start-up funds, Craig H. Neilsen Foundation awards","doi-asserted-by":"publisher","award":["385446"],"award-info":[{"award-number":["385446"]}],"id":[{"id":"10.13039\/100005191","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005191","name":"FIU start-up funds, Craig H. 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Neilsen Foundation awards","doi-asserted-by":"publisher","award":["DoD HT9425-25-1-0376"],"award-info":[{"award-number":["DoD HT9425-25-1-0376"]}],"id":[{"id":"10.13039\/100005191","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human induced pluripotent stem cells (hiPSCs) hold great potential for patient-specific therapies. Transplantation of hiPSC-derived neural progenitor cells (NPCs) is a promising reparative strategy for spinal cord injury (SCI), but clinical translation requires efficient differentiation into desired neural lineages and purification before transplantation. Here, differentiated hiPSCs\u2014reprogrammed from human skin fibroblasts using Sendai virus-mediated expression of OCT4, SOX2, KLF4, and C-MYC\u2014into neural rosettes expressing SOX1 and PAX6, followed by neuronal precursors (\u03b2-tubulin III+\/NESTIN+) and glial precursors (GFAP+\/NESTIN+). Both neuronal and glial precursors expressed the A2B5 surface antigen. A2B5+ NPCs, purified by fluorescence-activated cell sorting (FACS), proliferated in vitro with mitogens, and differentiated into mature neurons and astrocytes under lineage-specific conditions. Then, NOD-SCID mice received a T9 contusion injury followed by transplantation of A2B5+ NPCs, human fibroblasts, or control medium at 8 days post-injury. At two months, grafted NPCs showed robust survival, progressive neuronal maturation (\u03b2-tubulin III+\u2192doublecortin+\u2192NeuN+), and astrocytic differentiation (GFAP+), particularly in spared white matter. Transplantation significantly increased spared white matter volume and improved hindlimb locomotor recovery, with no teratoma formation observed. These results demonstrate that hiPSC-derived, FACS-purified A2B5+ NPCs can survive, differentiate into neurons and astrocytes, and enhance functional recovery after SCI. This approach offers a safe and effective candidate cell source for treating SCI and potentially other neurological disorders.<\/jats:p>","DOI":"10.3390\/ijms26188940","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T07:33:02Z","timestamp":1758007982000},"page":"8940","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Recovery by Transplantation of Human iPSC-Derived A2B5 Positive Neural Progenitor Cell After Spinal Cord Injury in Mice"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1512-384X","authenticated-orcid":false,"given":"Yiyan","family":"Zheng","sequence":"first","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3723-513X","authenticated-orcid":false,"given":"Xiaohui","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4097-490X","authenticated-orcid":false,"given":"Ping","family":"Bu","sequence":"additional","affiliation":[{"name":"Vivian L Smith Department of Neurosurgery, University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haipeng","family":"Xue","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Vivian L Smith Department of Neurosurgery, University of Texas Health Science Center at Houston, Houston, TX 77030, USA"},{"name":"Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Zhou","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xugang","family":"Xia","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1875-8293","authenticated-orcid":false,"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5572-3108","authenticated-orcid":false,"given":"Qilin","family":"Cao","sequence":"additional","affiliation":[{"name":"Center for Translational Science, Florida International University, 11350 SW Village Pkwy, Port St. Lucie, FL 34987, USA"},{"name":"Department of Environmental Health Sciences, Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.neurom.2022.01.010","article-title":"Rehabilitation of People With Chronic Spinal Cord Injury Using a Laparoscopically Implanted Neurostimulator: Impact on Mobility and Urinary, Anorectal, and Sexual Functions","volume":"26","author":"Lemos","year":"2023","journal-title":"Neuromodulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111472","DOI":"10.1016\/j.injury.2024.111472","article-title":"Management of traumatic spinal cord injury: A current concepts review of contemporary and future treatment","volume":"55","author":"Baroudi","year":"2024","journal-title":"Injury"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4103\/1673-5374.317961","article-title":"Delving into the recent advancements of spinal cord injury treatment: A review of recent progress","volume":"17","author":"Flack","year":"2022","journal-title":"Neural Regen. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/S1474-4422(21)00464-6","article-title":"Cell-based and stem-cell-based treatments for spinal cord injury: Evidence from clinical trials","volume":"21","author":"Zipser","year":"2022","journal-title":"Lancet Neurol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1038\/s41583-020-0314-2","article-title":"Transplanting neural progenitor cells to restore connectivity after spinal cord injury","volume":"21","author":"Fischer","year":"2020","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104207","DOI":"10.1016\/j.bas.2025.104207","article-title":"Stem cell therapies for spinal cord injury in humans: A review of recent clinical research","volume":"5","author":"Sugai","year":"2025","journal-title":"Brain Spine"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6","DOI":"10.21037\/sci-2022-037","article-title":"Current status and prospects of regenerative medicine for spinal cord injury using human induced pluripotent stem cells: A review","volume":"10","author":"Inoue","year":"2023","journal-title":"Stem Cell Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1093\/brain\/awad392","article-title":"Neural stem cell therapies for spinal cord injury repair: An update on recent preclinical and clinical advances","volume":"147","author":"Hosseini","year":"2024","journal-title":"Brain"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1093\/stcltm\/szad044","article-title":"Human Spinal Oligodendrogenic Neural Progenitor Cells Enhance Pathophysiological Outcomes and Functional Recovery in a Clinically Relevant Cervical Spinal Cord Injury Rat Model","volume":"12","author":"Pieczonka","year":"2023","journal-title":"Stem Cells Transl. Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Salazar, D.L., Uchida, N., Hamers, F.P., Cummings, B.J., and Anderson, A.J. (2010). Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0012272"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1016\/j.cell.2012.08.020","article-title":"Long-distance growth and connectivity of neural stem cells after severe spinal cord injury","volume":"150","author":"Lu","year":"2012","journal-title":"Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/nm.4502","article-title":"Restorative effects of human neural stem cell grafts on the primate spinal cord","volume":"24","author":"Rosenzweig","year":"2018","journal-title":"Nat. Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Gallegos, C.M., Xue, H., Li, S., Kim, D.H., Zhou, H., Xia, X., Liu, Y., and Cao, Q. (2022). Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Promotes Forelimb Functional Recovery after Cervical Spinal Cord Injury. Cells, 11.","DOI":"10.3390\/cells11172765"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kobayashi, Y., Okada, Y., Itakura, G., Iwai, H., Nishimura, S., Yasuda, A., Nori, S., Hikishima, K., Konomi, T., and Fujiyoshi, K. (2012). Pre-evaluated safe human iPSC-derived neural stem cells promote functional recovery after spinal cord injury in common marmoset without tumorigenicity. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0052787"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"16825","DOI":"10.1073\/pnas.1108077108","article-title":"Grafted human-induced pluripotent stem-cell-derived neurospheres promote motor functional recovery after spinal cord injury in mice","volume":"108","author":"Nori","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"14069","DOI":"10.1073\/pnas.0507063102","article-title":"Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice","volume":"102","author":"Cummings","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1186\/scrt209","article-title":"Amelioration of motor\/sensory dysfunction and spasticity in a rat model of acute lumbar spinal cord injury by human neural stem cell transplantation","volume":"4","author":"Leerink","year":"2013","journal-title":"Stem Cell Res. Ther."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1016\/j.stem.2018.05.014","article-title":"A First-in-Human, Phase I Study of Neural Stem Cell Transplantation for Chronic Spinal Cord Injury","volume":"22","author":"Curtis","year":"2018","journal-title":"Cell Stem Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.1172\/JCI92955","article-title":"Prolonged human neural stem cell maturation supports recovery in injured rodent CNS","volume":"127","author":"Lu","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/j.stem.2012.12.015","article-title":"Forebrain engraftment by human glial progenitor cells enhances synaptic plasticity and learning in adult mice","volume":"12","author":"Han","year":"2013","journal-title":"Cell Stem Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4694","DOI":"10.1523\/JNEUROSCI.0311-05.2005","article-title":"Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury","volume":"25","author":"Keirstead","year":"2005","journal-title":"J. Neurosci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.neuron.2014.07.014","article-title":"Long-distance axonal growth from human induced pluripotent stem cells after spinal cord injury","volume":"83","author":"Lu","year":"2014","journal-title":"Neuron"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"12704","DOI":"10.1073\/pnas.0910106107","article-title":"Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury","volume":"107","author":"Tsuji","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1002\/stem.1083","article-title":"Treatment of a mouse model of spinal cord injury by transplantation of human induced pluripotent stem cell-derived long-term self-renewing neuroepithelial-like stem cells","volume":"30","author":"Fujimoto","year":"2012","journal-title":"Stem Cells"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"22","DOI":"10.22603\/ssrr.2023-0135","article-title":"Regenerative Medicine for Spinal Cord Injury Using Induced Pluripotent Stem Cells","volume":"8","author":"Nagoshi","year":"2024","journal-title":"Spine Surg. Relat. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zeng, C.W. (2025). Stem Cell-Based Approaches for Spinal Cord Injury: The Promise of iPSCs. Biology, 14.","DOI":"10.3390\/biology14030314"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","article-title":"Induction of pluripotent stem cells from adult human fibroblasts by defined factors","volume":"131","author":"Takahashi","year":"2007","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"348","DOI":"10.2183\/pjab.85.348","article-title":"Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome","volume":"85","author":"Fusaki","year":"2009","journal-title":"Proc. Jpn. Acad. Ser. B Phys. Biol. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"14234","DOI":"10.1073\/pnas.1103509108","article-title":"Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors","volume":"108","author":"Ban","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.scr.2017.01.004","article-title":"Human neural progenitors derived from integration-free iPSCs for SCI therapy","volume":"19","author":"Liu","year":"2017","journal-title":"Stem Cell Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"100317","DOI":"10.1016\/j.crmeth.2022.100317","article-title":"Improved Sendai viral system for reprogramming to naive pluripotency","volume":"2","author":"Kunitomi","year":"2022","journal-title":"Cell Rep. Methods"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1016\/j.stemcr.2015.01.006","article-title":"Long-term safety issues of iPSC-based cell therapy in a spinal cord injury model: Oncogenic transformation with epithelial-mesenchymal transition","volume":"4","author":"Nori","year":"2015","journal-title":"Stem Cell Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/nbt.1554","article-title":"Variation in the safety of induced pluripotent stem cell lines","volume":"27","author":"Miura","year":"2009","journal-title":"Nat. Biotechnol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1038\/nm.3267","article-title":"Tumorigenicity as a clinical hurdle for pluripotent stem cell therapies","volume":"19","author":"Lee","year":"2013","journal-title":"Nat. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4913","DOI":"10.1073\/pnas.76.10.4913","article-title":"Monoclonal antibody to a plasma membrane antigen of neurons","volume":"76","author":"Eisenbarth","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/glia.10111","article-title":"Oligodendrocyte and astrocyte development in rodents: An in situ and immunohistological analysis during embryonic development","volume":"40","author":"Liu","year":"2002","journal-title":"Glia"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3996","DOI":"10.1073\/pnas.95.7.3996","article-title":"A tripotential glial precursor cell is present in the developing spinal cord","volume":"95","author":"Rao","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lepore, A.C., O\u2019Donnell, J., Kim, A.S., Williams, T., Tuteja, A., Rao, M.S., Kelley, L.L., Campanelli, J.T., and Maragakis, N.J. (2011). Human glial-restricted progenitor transplantation into cervical spinal cord of the SOD1 mouse model of ALS. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0025968"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.stemcr.2015.11.013","article-title":"Grafted Human iPS Cell-Derived Oligodendrocyte Precursor Cells Contribute to Robust Remyelination of Demyelinated Axons after Spinal Cord Injury","volume":"6","author":"Kawabata","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.stemcr.2016.08.015","article-title":"Pretreatment with a gamma-Secretase Inhibitor Prevents Tumor-like Overgrowth in Human iPSC-Derived Transplants for Spinal Cord Injury","volume":"7","author":"Okubo","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1186\/s13041-020-00662-w","article-title":"Cell therapy for spinal cord injury by using human iPSC-derived region-specific neural progenitor cells","volume":"13","author":"Kajikawa","year":"2020","journal-title":"Mol. Brain"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1016\/j.stemcr.2018.10.022","article-title":"Treatment with a Gamma-Secretase Inhibitor Promotes Functional Recovery in Human iPSC- Derived Transplants for Chronic Spinal Cord Injury","volume":"11","author":"Okubo","year":"2018","journal-title":"Stem Cell Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1186\/s13287-021-02217-9","article-title":"hiPSC-derived NSCs effectively promote the functional recovery of acute spinal cord injury in mice","volume":"12","author":"Kong","year":"2021","journal-title":"Stem Cell Res. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Bonilla, P., Hernandez, J., Giraldo, E., Gonzalez-Perez, M.A., Alastrue-Agudo, A., Elkhenany, H., Vicent, M.J., Navarro, X., Edel, M., and Moreno-Manzano, V. (2021). Human-Induced Neural and Mesenchymal Stem Cell Therapy Combined with a Curcumin Nanoconjugate as a Spinal Cord Injury Treatment. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115966"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Shiga, Y., Shiga, A., Mesci, P., Kwon, H., Brifault, C., Kim, J.H., Jeziorski, J.J., Nasamran, C., Ohtori, S., and Muotri, A.R. (2019). Tissue-type plasminogen activator-primed human iPSC-derived neural progenitor cells promote motor recovery after severe spinal cord injury. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-55132-8"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.biomaterials.2015.12.032","article-title":"Injectable hydrogel promotes early survival of induced pluripotent stem cell-derived oligodendrocytes and attenuates longterm teratoma formation in a spinal cord injury model","volume":"83","author":"Fuhrmann","year":"2016","journal-title":"Biomaterials"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1186\/s13287-015-0255-2","article-title":"Comparison of intraspinal and intrathecal implantation of induced pluripotent stem cell-derived neural precursors for the treatment of spinal cord injury in rats","volume":"6","author":"Amemori","year":"2015","journal-title":"Stem Cell Res. Ther."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.3727\/096368914X684042","article-title":"Beneficial Effect of Human Induced Pluripotent Stem Cell-Derived Neural Precursors in Spinal Cord Injury Repair","volume":"24","author":"Romanyuk","year":"2015","journal-title":"Cell Transpl."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.stemcr.2017.02.003","article-title":"Fail-Safe System against Potential Tumorigenicity after Transplantation of iPSC Derivatives","volume":"8","author":"Itakura","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Itakura, G., Kobayashi, Y., Nishimura, S., Iwai, H., Takano, M., Iwanami, A., Toyama, Y., Okano, H., and Nakamura, M. (2015). Controlling immune rejection is a fail-safe system against potential tumorigenicity after human iPSC-derived neural stem cell transplantation. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0116413"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.stem.2012.09.013","article-title":"Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes","volume":"12","author":"Tohyama","year":"2013","journal-title":"Cell Stem Cell"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cmet.2016.03.001","article-title":"Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells","volume":"23","author":"Tohyama","year":"2016","journal-title":"Cell Metab."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/sctm.18-0096","article-title":"Selective Ablation of Tumorigenic Cells Following Human Induced Pluripotent Stem Cell-Derived Neural Stem\/Progenitor Cell Transplantation in Spinal Cord Injury","volume":"8","author":"Kojima","year":"2019","journal-title":"Stem Cells Transl. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.stem.2020.07.007","article-title":"Neural Stem Cell Grafts Form Extensive Synaptic Networks that Integrate with Host Circuits after Spinal Cord Injury","volume":"27","author":"Ceto","year":"2020","journal-title":"Cell Stem Cell"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1016\/j.stemcr.2017.04.004","article-title":"Comprehensive Monosynaptic Rabies Virus Mapping of Host Connectivity with Neural Progenitor Grafts after Spinal Cord Injury","volume":"8","author":"Adler","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2633105520974000","DOI":"10.1177\/2633105520974000","article-title":"Regeneration of Corticospinal Axons into Neural Progenitor Cell Grafts After Spinal Cord Injury","volume":"15","author":"Poplawski","year":"2020","journal-title":"Neurosci. Insights"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/nm.4066","article-title":"Spinal cord reconstitution with homologous neural grafts enables robust corticospinal regeneration","volume":"22","author":"Kadoya","year":"2016","journal-title":"Nat. Med."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1002\/sctm.19-0135","article-title":"The leading edge: Emerging neuroprotective and neuroregenerative cell-based therapies for spinal cord injury","volume":"9","author":"Ahuja","year":"2020","journal-title":"Stem Cells Transl. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/S0014-4886(03)00037-2","article-title":"Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury","volume":"181","author":"Lu","year":"2003","journal-title":"Exp. Neurol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1089\/scd.2011.0596","article-title":"An in vivo characterization of trophic factor production following neural precursor cell or bone marrow stromal cell transplantation for spinal cord injury","volume":"21","author":"Hawryluk","year":"2012","journal-title":"Stem Cells Dev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1038\/nn.3163","article-title":"Cross-talk between neural stem cells and immune cells: The key to better brain repair?","volume":"15","author":"Kokaia","year":"2012","journal-title":"Nat. Neurosci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/s41419-019-1571-8","article-title":"Neural stem cell-derived small extracellular vesicles attenuate apoptosis and neuroinflammation after traumatic spinal cord injury by activating autophagy","volume":"10","author":"Rong","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"114779","DOI":"10.1016\/j.expneurol.2024.114779","article-title":"An improved method for generating human spinal cord neural stem cells","volume":"376","author":"Li","year":"2024","journal-title":"Exp. Neurol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1038\/s41592-018-0074-3","article-title":"Generation and post-injury integration of human spinal cord neural stem cells","volume":"15","author":"Kumamaru","year":"2018","journal-title":"Nat. Methods"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.reth.2021.08.005","article-title":"First-in-human clinical trial of transplantation of iPSC-derived NS\/PCs in subacute complete spinal cord injury: Study protocol","volume":"18","author":"Sugai","year":"2021","journal-title":"Regen. Ther."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.stem.2024.12.005","article-title":"Pluripotent stem-cell-derived therapies in clinical trial: A 2025 update","volume":"32","author":"Kirkeby","year":"2025","journal-title":"Cell Stem Cell"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.expneurol.2017.10.014","article-title":"Apolipoprotein E as a novel therapeutic neuroprotection target after traumatic spinal cord injury","volume":"299","author":"Cheng","year":"2018","journal-title":"Exp. Neurol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1523\/JNEUROSCI.3174-09.2010","article-title":"Transplantation of ciliary neurotrophic factor-expressing adult oligodendrocyte precursor cells promotes remyelination and functional recovery after spinal cord injury","volume":"30","author":"Cao","year":"2010","journal-title":"J. Neurosci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1089\/neu.2006.23.635","article-title":"Basso Mouse Scale for locomotion detects differences in recovery after spinal cord injury in five common mouse strains","volume":"23","author":"Basso","year":"2006","journal-title":"J. Neurotrauma"},{"key":"ref_70","first-page":"10","article-title":"Apolipoprotein E Mimetic Promotes Functional and Histological Recovery in Lysolecithin-Induced Spinal Cord Demyelination in Mice","volume":"2014","author":"Gu","year":"2013","journal-title":"J. Neurol. Neurophysiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/neu.2008.0543","article-title":"Anatomical and functional outcomes following a precise, graded, dorsal laceration spinal cord injury in C57BL\/6 mice","volume":"26","author":"Hill","year":"2009","journal-title":"J. Neurotrauma"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Gallegos, C., Carey, M., Zheng, Y., He, X., and Cao, Q.L. (2020). Reaching and Grasping Training Improves Functional Recovery After Chronic Cervical Spinal Cord Injury. Front. 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