{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:33Z","timestamp":1787028573341,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation de l\u2019Avenir (Paris, France)"},{"name":"University Hospital of Nantes (Nantes, France)"},{"name":"F\u00e9d\u00e9ration Fran\u00e7aise de Cardiologie (Paris, France)"},{"name":"Soci\u00e9t\u00e9 Francaise de Cardiologie (Paris, France)"},{"name":"Soci\u00e9t\u00e9 de Chirurgie Vasculaire et Endovasculaire (Paris, France)"},{"name":"Fondation Genavie"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Vascular calcification (V) is an independent risk factor for all-cause and cardiovascular mortality. Vascular smooth muscle cells (VSMCs) play a major role in VC as they can acquire mineralizing properties when exposed to osteogenic conditions. Despite its clinical impact, there are still no dedicated therapeutic strategies targeting VC. To address this issue, we used human calcified and non-calcified atherosclerotic arteries (ECLAGEN Biocollection) to screen and identify microRNA (miR) associated with VC. We combined non-biased miRNomic (microfluidic arrays) and transcriptomic analysis to select miR candidates and their putative target genes with expression associated with VC and ossification. We further validated miR functional regulation and function in relation to cell mineralization using primary human VSMCs. Our study identified 12 miRs associated with VC in carotid and femoral arteries. Among those, we showed that miR136, miR155, and miR183 expression were regulated during VSMC mineralization and that overexpression of these miRs promoted VSMC mineralization. Cross-analysis of this miRNomic and a transcriptomic analysis led to the identification of CD73 and Smad3 pathways as putative target genes responsible for mediating the miR155 pro-mineralizing function. These results highlight the potential benefit of miR155 inhibition in limiting VC development in peripheral atherosclerotic arteries.<\/jats:p>","DOI":"10.3390\/ijms26199349","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:56:44Z","timestamp":1758790604000},"page":"9349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification of miR136, miR155, and miR183 in Vascular Calcification in Human Peripheral Arteries"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5444-4787","authenticated-orcid":false,"given":"Tom","family":"Le Corvec","sequence":"first","affiliation":[{"name":"Centre Hospitalier Universitaire de Nantes, L\u2019institut du Thorax, Service de Chirurgie Vasculaire, 44000 Nantes, France"},{"name":"Nantes Universit\u00e9, CNRS, INSERM, L\u2019institut du Thorax, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5943-4960","authenticated-orcid":false,"given":"Mathilde","family":"Burgaud","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Clermont-Ferrand, H\u00f4pital Gabriel Montpied, 63003 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4547-4448","authenticated-orcid":false,"given":"Marja","family":"Steenman","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CNRS, INSERM, L\u2019institut du Thorax, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2358-219X","authenticated-orcid":false,"given":"Robel A.","family":"Tesfaye","sequence":"additional","affiliation":[{"name":"Laboratory of Chromatin Dynamics, Centre de Biologie Int\u00e9grative (CBI), MCD Unit (UMR5077), Universit\u00e9 de Toulouse, CNRS, UPS, 31062 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann","family":"Gou\u00ebffic","sequence":"additional","affiliation":[{"name":"Groupe Hospitalier Paris St. Joseph, Department of Vascular and Endovascular Surgery, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-6951","authenticated-orcid":false,"given":"Blandine","family":"Maurel","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Universitaire de Nantes, L\u2019institut du Thorax, Service de Chirurgie Vasculaire, 44000 Nantes, France"},{"name":"Nantes Universit\u00e9, CNRS, INSERM, L\u2019institut du Thorax, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibaut","family":"Quillard","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CNRS, INSERM, L\u2019institut du Thorax, 44000 Nantes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1177\/0003319718796889","article-title":"Association Between Abdominal Visceral Artery Calcification and All-Cause Mortality-A Computerized Tomography Imaging-Based Longitudinal Follow-Up Study","volume":"70","author":"Sadat","year":"2019","journal-title":"Angiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ejvs.2017.11.007","article-title":"Editor\u2019s Choice\u2014Calcification of Thoracic and Abdominal Aneurysms is Associated with Mortality and Morbidity","volume":"55","author":"Chowdhury","year":"2018","journal-title":"Eur. 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