{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:00:26Z","timestamp":1759334426273,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundaci\u00f3n Seneca","award":["21756\/IV\/22"],"award-info":[{"award-number":["21756\/IV\/22"]}]},{"name":"MDPI"},{"name":"Fundaci\u00f3n Seneca-Murcia Regional Agency for Science and Technology","award":["20855\/PI\/18"],"award-info":[{"award-number":["20855\/PI\/18"]}]},{"name":"European Union NextGenerationEU","award":["PRTR-C17.I1"],"award-info":[{"award-number":["PRTR-C17.I1"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Plants from the Brassicaceae family are characterized by their high content of glucosinolates (GSLs), whose hydrolysis products, isothiocyanates (ITC) or indole compounds, have been found to have anti-inflammatory, antioxidant and metabolic regulatory functions. In this work, we used a model of transformation using the MCF10A cell line, a non-tumorigenic breast fibrocystic disease cell line, treated with benzo(a)pyrene (B(a)P), a potent carcinogen known to induce the production of reactive oxygen species (ROS) and DNA damage. Broccoli sprout (BSE) or red cabbage aqueous (RCA) extracts were rich in ITC and indole compounds. Their use decreased B(a)P induced cellular proliferation and ROS production. in addition, RCA extract induced autophagy in MCF10A cells. Our results indicate a potential use of BSE or RCA for the prevention of carcinogen-induced transformation and of RCA as a method for autophagy, a tumor suppressor pathway, induction.<\/jats:p>","DOI":"10.3390\/ijms26199519","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T15:38:55Z","timestamp":1759246735000},"page":"9519","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Brassica Extracts Prevent Benzo(a)pyrene-Induced Transformation by Modulating Reactive Oxygen Species and Autophagy"],"prefix":"10.3390","volume":"26","author":[{"given":"Jos\u00e9 Benito","family":"Montes-Alvarado","sequence":"first","affiliation":[{"name":"Biotechnology Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2117-3130","authenticated-orcid":false,"given":"Paula","family":"Garcia-Iba\u00f1ez","sequence":"additional","affiliation":[{"name":"Aquaporins Group, Plant Nutrition Department, Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura, Consejo Superior de Investigaciones Cient\u00edficas, Campus Universitario de Espinardo-25, E-30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6547-8764","authenticated-orcid":false,"given":"Diego A.","family":"Moreno","sequence":"additional","affiliation":[{"name":"Phytochemistry and Healthy Food Lab (LabFAS), Centro de Edafolog\u00eda y Biolog\u00eda Aplicada del Segura, Consejo Superior de Investigaciones Cient\u00edficas, Campus Universitario de Espinardo-25, E-30100 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola Lil\u00ed","family":"Sarmiento-Salinas","sequence":"additional","affiliation":[{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n (SECIHTI), Mexico City 03940, Mexico"},{"name":"Metabolic Biochemistry Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiadani Ed\u00e9n","family":"Susano-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Metabolic Biochemistry Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"},{"name":"Facultad de Ciencias Biol\u00f3gicas, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8049-8352","authenticated-orcid":false,"given":"Karen Andrea","family":"Larrauri-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Metabolic Biochemistry Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"},{"name":"Facultad de Ciencias Biol\u00f3gicas, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Jes\u00fas","family":"Garc\u00eda-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Metabolic Biochemistry Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"},{"name":"Programa de Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica de Puebla, Puebla 72640, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Milflores-Flores","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Benem\u00e9rita Universidad Aut\u00f3noma de Puebla, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiola","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"Biotechnology Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4059-0554","authenticated-orcid":false,"given":"Paola","family":"Maycotte","sequence":"additional","affiliation":[{"name":"Metabolic Biochemistry Laboratory, Centro de Investigaci\u00f3n Biom\u00e9dica de Oriente, Instituto Mexicano del Seguro Social, Puebla 74360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abdel-Massih, R.M., Debs, E., Othman, L., Attieh, J., and Cabrerizo, F.M. 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