{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T13:48:54Z","timestamp":1784814534594,"version":"3.55.0"},"reference-count":51,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004881","name":"Instituto Mexicano del Seguro Social","doi-asserted-by":"publisher","award":["R-2023-785-020"],"award-info":[{"award-number":["R-2023-785-020"]}],"id":[{"id":"10.13039\/501100004881","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Placenta and visceral adipose tissue (VAT) are implicated in the development of gestational diabetes mellitus (GDM). In the present study, we examined the whole-transcriptomic profile of both tissues in GDM women to elucidate the molecular basis of GDM pathogenesis. The whole-transcriptome profile was analyzed in placenta and VAT from at-term patients with GDM and controls using RNA-seq. qPCR was used to validate several differentially expressed genes (DEGs). A total of 179 DEGs were observed in the placenta and 4 in VAT, including both up- and downregulated genes. The expression of the selected mRNAs for validation was consistent with the sequencing results. An analysis of the placental upregulated DEGs in the GDM women showed enrichment in functions including the G-protein-coupled receptor signaling pathway, organophosphate biosynthetic process, and phospholipid metabolic process, while the downregulated DEGs were enriched in cell motility and the cell migration process. The target pathways of DEGs in VAT are related to cancer and to the activation of the complement cascade. Molecular pathways involved in G-protein-coupled receptor signaling, the organophosphate biosynthetic process, the phospholipid metabolic process, and cell motility and cell migration are altered in the placentas of GDM women. Moreover, a disordered complement cascade might take place in the VAT of GDM women.<\/jats:p>","DOI":"10.3390\/ijms26199595","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T11:06:02Z","timestamp":1759316762000},"page":"9595","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gene Expression Profile of Placenta and Adipose Tissue in Women with Gestational Diabetes Mellitus"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5167-6156","authenticated-orcid":false,"given":"Renata","family":"Saucedo","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Endocrinas, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3637-4325","authenticated-orcid":false,"given":"Erika","family":"Magall\u00f3n-Gay\u00f3n","sequence":"additional","affiliation":[{"name":"Escuela de Ingenier\u00eda y Ciencias, Campus Monterrey, Tecnol\u00f3gico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4551-1862","authenticated-orcid":false,"given":"Rocio Alejandra","family":"Chavez-Santoscoy","sequence":"additional","affiliation":[{"name":"Escuela de Ingenier\u00eda y Ciencias, Campus Monterrey, Tecnol\u00f3gico de Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey 64700, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Flor","family":"D\u00edaz-Vel\u00e1zquez","sequence":"additional","affiliation":[{"name":"Hospital de Gineco Obstetricia 3, Centro M\u00e9dico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City 02990, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5159-9856","authenticated-orcid":false,"given":"Aldo","family":"Ferreira-Hermosillo","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Endocrinas, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7395-9780","authenticated-orcid":false,"given":"Diana","family":"Ojeda-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Hospital de Gineco Obstetricia 3, Centro M\u00e9dico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City 02990, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5830-8133","authenticated-orcid":false,"given":"Wendy","family":"Porras-Marcial","sequence":"additional","affiliation":[{"name":"Hospital de Gineco Obstetricia 3, Centro M\u00e9dico Nacional La Raza, Instituto Mexicano del Seguro Social, Mexico City 02990, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5797-6131","authenticated-orcid":false,"given":"Debbie","family":"L\u00f3pez-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Estudios de Posgrado, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Valencia-Ortega","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica en Enfermedades Endocrinas, Hospital de Especialidades, Centro M\u00e9dico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City 06720, Mexico"},{"name":"Laboratorio de Investigaci\u00f3n en Biolog\u00eda Molecular, Hospital Infantil de M\u00e9xico \u201cFederico G\u00f3mez,\u201d Mexico City 06720, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,1]]},"reference":[{"key":"ref_1","unstructured":"American Diabetes Association Professional Practice Committee (2025). 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