{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T15:09:09Z","timestamp":1788448149528,"version":"build-2803163510"},"reference-count":61,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto de Investigaci\u00f3n Marqu\u00e9s Valdecilla","award":["NVAL24\/10"],"award-info":[{"award-number":["NVAL24\/10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a significant public health concern, yet rare sublineages remain poorly characterized. Here, we described a K30-ST198 hvKp sublineage identified in four isolates from two patients, including three sequential strains (K30B1, K30B2, K30B3) recovered over eight months from recurrent liver abscesses and one strain (K30-HUMV1) from a urinary tract infection. All isolates exhibited a yYpermucoviscous phenotype and resistance restricted to ampicillin and amoxicillin. Screening with the eazyplex hvKp assay detected ybt and rmpA in all strains, yielding a virulence score of 1. Biofilm production was strong in K30B1, K30B2, moderate in K30-HUMV1, but weak in K30B3. In the Galleria mellonella infection model, K30B1 showed higher virulence than the other isolates. Whole-genome sequencing identified the ICEKp1 carrying hypervirulence-associated genes (ybt, pagO, rmpAC, iroBCDN) together with additional virulence factors (fim, mrkD, uge, ureA, wabG, wcaJ, mliC), while antibiotic resistance genes were limited to fosA and blaSHV-77. Protein structures and their functional domains were predicted using AlphaFold v3.0.1 and ColabFold v1.5.5, based on pLDDT scores, providing further insights into gene functionality. This work represents one of the first detailed characterizations of K30-ST198 hvKp, underscoring the need for integrated genomic, phenotypic, and structural approaches in hvKp surveillance.<\/jats:p>","DOI":"10.3390\/ijms26199601","type":"journal-article","created":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T12:22:03Z","timestamp":1759321323000},"page":"9601","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic Characterization of a Rare K30-ST198 Hypervirulent Klebsiella pneumoniae Clone with Distinctive Virulence Features"],"prefix":"10.3390","volume":"26","author":[{"given":"Domingo","family":"Fern\u00e1ndez Vecilla","sequence":"first","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Rodr\u00edguez Grande","sequence":"additional","affiliation":[{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Fraile Valc\u00e1rcel","sequence":"additional","affiliation":[{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5819-0148","authenticated-orcid":false,"given":"Mary Paz","family":"Roche Matheus","sequence":"additional","affiliation":[{"name":"Basurto University Hospital, Montevideo Etorb., 18, Basurtu-Zorrotza, 48013 Bilbao, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gotzon","family":"Iglesias Hidalgo","sequence":"additional","affiliation":[{"name":"Cruces University Hospital, Cruces Plaza, 12, 48903 Barakaldo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Aspichueta Vivanco","sequence":"additional","affiliation":[{"name":"Basurto University Hospital, Montevideo Etorb., 18, Basurtu-Zorrotza, 48013 Bilbao, Spain"},{"name":"Bioruces Health Research Institute, Cruces Plaza, 48903 Barakaldo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0095-7430","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"D\u00edaz de Tuesta del Arco","sequence":"additional","affiliation":[{"name":"Basurto University Hospital, Montevideo Etorb., 18, Basurtu-Zorrotza, 48013 Bilbao, Spain"},{"name":"Bioruces Health Research Institute, Cruces Plaza, 48903 Barakaldo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Garc\u00eda-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Siller Ruiz","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaira","family":"Moure","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Vallejo","family":"Iriarte","sequence":"additional","affiliation":[{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6739-8859","authenticated-orcid":false,"given":"Athanasia","family":"Varsaki","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Formaci\u00f3n Agrarias (CIFA), 39600 Muriedas, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Calvo Montes","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8677-8128","authenticated-orcid":false,"given":"Mar\u00eda P\u00eda","family":"Roiz Mesones","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Carmen","family":"Fari\u00f1as","sequence":"additional","affiliation":[{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"},{"name":"Infectious Diseases Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9104-3115","authenticated-orcid":false,"given":"Alain A.","family":"Ocampo-Sosa","sequence":"additional","affiliation":[{"name":"Microbiology Service, University Hospital Marqu\u00e9s de Valdecilla, Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"Marqu\u00e9s de Valdecilla Research Institute (IDIVAL), Av. Valdecilla s\/n, 39008 Santander, Spain"},{"name":"CIBERINFEC, Health Institute Carlos III, Monforte de Lemos, 5, 28020 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"107","DOI":"10.4161\/viru.22718","article-title":"Hypervirulent (hypermucoviscous) Klebsiella pneumoniae: A new and dangerous breed","volume":"4","author":"Shon","year":"2013","journal-title":"Virulence"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/S1473-3099(12)70205-0","article-title":"Klebsiella pneumoniae liver abscess: A new invasive syndrome","volume":"12","author":"Siu","year":"2012","journal-title":"Lancet Infect. 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