{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T19:06:20Z","timestamp":1782327980948,"version":"3.54.5"},"reference-count":87,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["23-15-00357"],"award-info":[{"award-number":["23-15-00357"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Russian state-funded project for ICBFM SB RAS","award":["125012300658-9"],"award-info":[{"award-number":["125012300658-9"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["IJMS"],"abstract":"<jats:p>Antinuclear antibodies, especially anti-DNA antibodies, are known to be a hallmark of systemic lupus erythematosus (SLE) and represent a diverse pool of autoantibodies with different origins, antigenic properties, and physicochemical features. Antibodies with catalytic properties have been found among the antibody repertoire in SLE, but the specific features and clinical associations of such antibodies have not been sufficiently studied. This study showed that chromatographically purified IgG from the serum of SLE patients effectively hydrolyzed DNA and DNA-associated proteins such as histones and high-mobility group box 1 (HMGB1) compared to healthy individuals. Remarkably, the level of hydrolysis of DNA and DNA-associated proteins was closely correlated. At the same time, these antibodies did not hydrolyze the control protein, tumor necrosis factor-\u03b1 (TNF\u03b1), which does not possess DNA-binding properties. IgG DNase activity levels varied significantly, so patients were divided into high- and low-activity subgroups using the DBSCAN algorithm, with the difference between median values being greater than 49 times. The subgroup with high IgG DNase activity was characterized by an increase in anti-DNA antibodies (p &lt; 0.04) than the subgroup with low activity, which had a shorter duration of the disease (p = 0.03) and was more often characterized by a subacute rather than a non-chronic course of the disease (p = 0.048). High catalase-like activity of IgG was also detected in SLE. Thus, the antibody pool in SLE contains not only high-affinity antinuclear autoantibodies but also catalytic antibodies capable of hydrolyzing DNA and DNA-associated proteins. These findings expand our understanding of the heterogeneity of the repertoire of catalytic autoantibodies among SLE patients.<\/jats:p>","DOI":"10.3390\/ijms26199635","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:18:33Z","timestamp":1759421913000},"page":"9635","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Catalytic IgG Antibodies Hydrolyze DNA, Histones, and HMGB1 in Systemic Lupus Erythematosus"],"prefix":"10.3390","volume":"26","author":[{"given":"Mark M.","family":"Melamud","sequence":"first","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1084-6419","authenticated-orcid":false,"given":"Evgeny A.","family":"Ermakov","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0984-0810","authenticated-orcid":false,"given":"Anna S.","family":"Tolmacheva","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0987-7066","authenticated-orcid":false,"given":"Irina A.","family":"Kostrikina","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7213-7482","authenticated-orcid":false,"given":"Alexey E.","family":"Sizikov","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Department of Rheumatology, Immunopathology Clinic, Research Institute of Fundamental and Clinical Immunology, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4988-8923","authenticated-orcid":false,"given":"Georgy A.","family":"Nevinsky","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2556-979X","authenticated-orcid":false,"given":"Valentina N.","family":"Buneva","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nrrheum.2016.137","article-title":"The Global Burden of SLE: Prevalence, Health Disparities and Socioeconomic Impact","volume":"12","author":"Carter","year":"2016","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1093\/rheumatology\/ken477","article-title":"Employment and Work Disability in Systemic Lupus Erythematosus: A Systematic Review","volume":"48","author":"Baker","year":"2008","journal-title":"Rheumatology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1136\/ard.2005.046367","article-title":"Cost of Illness in Rheumatoid Arthritis, Ankylosing Spondylitis, Psoriatic Arthritis and Systemic Lupus Erythematosus in Germany","volume":"65","author":"Huscher","year":"2006","journal-title":"Ann. Rheum. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"103648","DOI":"10.1016\/j.autrev.2024.103648","article-title":"Immunopathogenesis of Systemic Lupus Erythematosus: An Update","volume":"23","author":"Arnaud","year":"2024","journal-title":"Autoimmun. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s12969-024-00953-9","article-title":"Lymphocyte Apoptosis and Its Association with the Inflammatory Markers and Disease Severity in Juvenile-Onset Systemic Lupus Erythematosus Patients","volume":"22","author":"Eissa","year":"2024","journal-title":"Pediatr. Rheumatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e2350918","DOI":"10.1002\/eji.202350918","article-title":"Canonical and Noncanonical Functions of Complement in Systemic Lupus Erythematosus","volume":"54","author":"Pickering","year":"2024","journal-title":"Eur. J. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Huang, J., Li, X., Zhu, Q., Wang, M., Xie, Z., and Zhao, T. (2024). Imbalance of Th17 Cells, Treg Cells and Associated Cytokines in Patients with Systemic Lupus Erythematosus: A Meta-Analysis. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1425847"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"109031","DOI":"10.1016\/j.clim.2022.109031","article-title":"T Cell Dysregulation in SLE","volume":"239","author":"Tenbrock","year":"2022","journal-title":"Clin. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1002\/art.40396","article-title":"Review: Abnormal B Cell Development in Systemic Lupus Erythematosus: What the Genetics Tell Us","volume":"70","author":"Karrar","year":"2018","journal-title":"Arthritis Rheumatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100139","DOI":"10.1016\/j.jtauto.2021.100139","article-title":"Anti-dsDNA Antibodies in the Classification Criteria of Systemic Lupus Erythematosus","volume":"5","author":"Infantino","year":"2022","journal-title":"J. Transl. Autoimmun."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wang, X., and Xia, Y. (2019). Anti-Double Stranded DNA Antibodies: Origin, Pathogenicity, and Targeted Therapies. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.01667"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kubota, T. (2023). An Emerging Role for Anti-DNA Antibodies in Systemic Lupus Erythematosus. Int. J. Mol. Sci., 24.","DOI":"10.20944\/preprints202310.2067.v1"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1016\/j.clinthera.2016.02.029","article-title":"The Alarmin Properties of DNA and DNA-Associated Nuclear Proteins","volume":"38","author":"Magna","year":"2016","journal-title":"Clin. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Duvvuri, B., and Lood, C. (2019). Cell-Free DNA as a Biomarker in Autoimmune Rheumatic Diseases. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00502"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Park, H., Kim, M., Seo, Y., Ham, Y., Cho, M.-Y., and Kwon, M.-H. (2018). Cytosolic Internalization of Anti-DNA Antibodies by Human Monocytes Induces Production of Pro-Inflammatory Cytokines Independently of the Tripartite Motif-Containing 21 (TRIM21)-Mediated Pathway. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.02019"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1007\/s00018-009-9179-2","article-title":"A Nucleic Acid-Hydrolyzing Antibody Penetrates into Cells via Caveolae-Mediated Endocytosis, Localizes in the Cytosol and Exhibits Cytotoxicity","volume":"66","author":"Jang","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.molimm.2015.06.025","article-title":"Cell- and Nuclear-Penetrating Anti-dsDNA Autoantibodies Have Multiple Arginines in CDR3 of VH and Increase Cellular Level of pERK and Bcl-2 in Mesangial Cells","volume":"67","author":"Im","year":"2015","journal-title":"Mol. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/cei.13382","article-title":"Monoclonal Anti-dsDNA Antibody 2C10 Escorts DNA to Intracellular DNA Sensors in Normal Mononuclear Cells and Stimulates Secretion of Multiple Cytokines Implicated in Lupus Pathogenesis","volume":"199","author":"Inoue","year":"2020","journal-title":"Clin. Exp. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2417","DOI":"10.1161\/ATVBAHA.121.315928","article-title":"Anti-dsDNA Antibodies Increase the Cardiovascular Risk in Systemic Lupus Erythematosus Promoting a Distinctive Immune and Vascular Activation","volume":"41","author":"Espinosa","year":"2021","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1038\/s41577-023-00894-6","article-title":"The Multifunctional Protein HMGB1: 50 Years of Discovery","volume":"23","author":"Tang","year":"2023","journal-title":"Nat. Rev. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.smim.2018.02.011","article-title":"High-Mobility Group Box 1 Protein (HMGB1) Operates as an Alarmin Outside as Well as inside Cells","volume":"38","author":"Andersson","year":"2018","journal-title":"Semin. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"R71","DOI":"10.1186\/ar3332","article-title":"High Mobility Group Box 1 (HMGB1) and Anti-HMGB1 Antibodies and Their Relation to Disease Characteristics in Systemic Lupus Erythematosus","volume":"13","author":"Abdulahad","year":"2011","journal-title":"Arthritis Res. Ther."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1186\/s13075-015-0856-2","article-title":"Antibodies against High Mobility Group Box Protein-1 (HMGB1) versus Other Anti-Nuclear Antibody Fine-Specificities and Disease Activity in Systemic Lupus Erythematosus","volume":"17","author":"Wirestam","year":"2015","journal-title":"Arthritis Res. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"283","DOI":"10.3109\/s10165-009-0151-7","article-title":"Lupus Antibodies to the HMGB1 Chromosomal Protein: Epitope Mapping and Association with Disease Activity","volume":"19","author":"Hayashi","year":"2009","journal-title":"Mod. Rheumatol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Dema, B., and Charles, N. (2016). Autoantibodies in SLE: Specificities, Isotypes and Receptors. Antibodies, 5.","DOI":"10.3390\/antib5010002"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.biopha.2004.04.011","article-title":"The Natural Autoantibody Repertoire and Autoimmune Disease","volume":"58","author":"Quintana","year":"2004","journal-title":"Biomed. Pharmacother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.jaci.2023.02.008","article-title":"Antibody Repertoire and Autoimmunity","volume":"151","author":"Skevaki","year":"2023","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1582-4934.2003.tb00227.x","article-title":"Catalytic Antibodies in Healthy Humans and Patients with Autoimmune and Viral Diseases","volume":"7","author":"Nevinsky","year":"2003","journal-title":"J. Cell. Mol. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S373","DOI":"10.1134\/S0006297924604167","article-title":"Immune Response and Production of Abzymes in Patients with Autoimmune and Neurodegenerative Diseases","volume":"90","author":"Nevinsky","year":"2025","journal-title":"Biochem. Mosc."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ermakov, E.A., Nevinsky, G.A., and Buneva, V.N. (2020). Immunoglobulins with Non-Canonical Functions in Inflammatory and Autoimmune Disease States. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155392"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.it.2020.03.006","article-title":"Noncanonical Functions of Antibodies","volume":"41","author":"Dimitrov","year":"2020","journal-title":"Trends Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.jaut.2011.05.007","article-title":"Autoantibodies with Enzymatic Properties in Human Autoimmune Diseases","volume":"37","author":"Wootla","year":"2011","journal-title":"J. Autoimmun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e202300078","DOI":"10.1002\/ijch.202300078","article-title":"Immunoglobulin Go: Synergy of Combinatorics for Catalysis","volume":"63","author":"Smirnov","year":"2023","journal-title":"Isr. J. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Bowen, A., Wear, M., and Casadevall, A. (2017). Antibody-Mediated Catalysis in Infection and Immunity. Infect. Immun., 85.","DOI":"10.1128\/IAI.00202-17"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.imlet.2005.10.003","article-title":"Theory of Proteolytic Antibody Occurrence","volume":"103","author":"Paul","year":"2006","journal-title":"Immunol. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1002\/bit.20031","article-title":"Catalytic Antibody Light Chain Capable of Cleaving a Chemokine Receptor CCR-5 Peptide with a High Reaction Rate Constant","volume":"86","author":"Mitsuda","year":"2004","journal-title":"Biotechnol. Bioeng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/S0022-2836(03)00902-1","article-title":"High-Resolution Crystal Structure of the Fab-Fragments of a Family of Mouse Catalytic Antibodies with Esterase Activity","volume":"332","author":"Ruzheinikov","year":"2003","journal-title":"J. Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1007\/s12010-022-04183-1","article-title":"Catalytic Antibodies: Design, Expression, and Their Applications in Medicine","volume":"195","author":"Zhao","year":"2023","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1126\/science.1585181","article-title":"DNA Hydrolyzing Autoantibodies","volume":"256","author":"Shuster","year":"1992","journal-title":"Science"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9887","DOI":"10.3390\/cimb45120617","article-title":"Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus Erythematosus","volume":"45","author":"Novikova","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/s41537-021-00143-6","article-title":"DNA Hydrolysing IgG Catalytic Antibodies: An Emerging Link between Psychoses and Autoimmunity","volume":"7","author":"Ramesh","year":"2021","journal-title":"Npj Schizophr."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5243","DOI":"10.1093\/nar\/26.23.5243","article-title":"Characterization and Selectivity of Catalytic Antibodies from Human Serum with RNase Activity","volume":"26","author":"Vlassov","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0165-2478(02)00006-8","article-title":"Catalytic Diversity of Polyclonal RNA-Hydrolyzing IgG Antibodies from the Sera of Patients with Systemic Lupus Erythematosus","volume":"81","author":"Andrievskaya","year":"2002","journal-title":"Immunol. Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"681","DOI":"10.2147\/JIR.S258558","article-title":"IgGs-Abzymes from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyzed miRNAs","volume":"13","author":"Ermakov","year":"2020","journal-title":"J. Inflamm. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4052\/tigg.16.17","article-title":"Human Abzymes with Amylolytic Activity","volume":"16","author":"Kulminskaya","year":"2004","journal-title":"Trends Glycosci. Glycotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1002\/jmr.1033","article-title":"Anti-histone H1 IgGs from Blood Serum of Systemic Lupus Erythematosus Patients Are Capable of Hydrolyzing Histone H1 and Myelin Basic Protein","volume":"23","author":"Magorivska","year":"2010","journal-title":"J. Mol. Recognit."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1093\/intimm\/dxs071","article-title":"IgGs Containing Light Chains of the \u03bb- and \u03ba- Type and of All Subclasses (IgG1\u2013IgG4) from the Sera of Patients with Systemic Lupus Erythematosus Hydrolyze Myelin Basic Protein","volume":"24","author":"Bezuglova","year":"2012","journal-title":"Int. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"173","DOI":"10.5152\/eurjrheum.2018.17194","article-title":"Catalytic Antibodies in Patients with Systemic Lupus Erythematosus","volume":"5","author":"Pradhan","year":"2018","journal-title":"Eur. J. Rheumatol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1002\/jmr.2474","article-title":"IgG Abzymes with Peroxidase and Oxidoreductase Activities from the Sera of Healthy Humans: Oxidoreductase Activities of Human IgGs","volume":"28","author":"Tolmacheva","year":"2015","journal-title":"J. Mol. Recognit."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Tolmacheva, A.S., Sedykh, S.E., Onvumere, M.K., Timofeeva, A.M., Maksimenko, L.V., Gashnikova, N.M., and Nevinsky, G.A. (2024). Catalase Activity of IgG and \u039a\u03ba-IgG, \u039b\u03bb-IgG, and \u039a\u03bb-IgG Subfractions in HIV-Infected Patients and Healthy Donors. Front. Biosci.-Landmark, 29.","DOI":"10.31083\/j.fbl2905191"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"171097","DOI":"10.1098\/rsos.171097","article-title":"Substrate Specificity of Healthy Human Sera IgG Antibodies with Peroxidase and Oxydoreductase Activities","volume":"5","author":"Tolmacheva","year":"2018","journal-title":"R. Soc. Open Sci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Ermakov, E.A., Smirnova, L.P., Bokhan, N.A., Semke, A.V., Ivanova, S.A., Buneva, V.N., and Nevinsky, G.A. (2017). Catalase Activity of IgG Antibodies from the Sera of Healthy Donors and Patients with Schizophrenia. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0183867"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Tolmacheva, A.S., Onvumere, M.K., Sedykh, S.E., Timofeeva, A.M., and Nevinsky, G.A. (2023). Catalase Activity of IgGs of Patients Infected with SARS-CoV-2. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241210081"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Tolmacheva, A.S., and Nevinsky, G.A. (2022). Essential Protective Role of Catalytically Active Antibodies (Abzymes) with Redox Antioxidant Functions in Animals and Humans. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073898"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.imlet.2017.01.018","article-title":"Differential Reactive Oxygen Species Production of Neutrophils and Their Oxidative Damage in Patients with Active and Inactive Systemic Lupus Erythematosus","volume":"184","author":"Elloumi","year":"2017","journal-title":"Immunol. Lett."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1651724","DOI":"10.1155\/2019\/1651724","article-title":"Systemic Lupus Erythematosus: Pathogenesis at the Functional Limit of Redox Homeostasis","volume":"2019","author":"Pravda","year":"2019","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ermakov, E.A., Smirnova, L.P., Parkhomenko, T.A., Dmitrenok, P.S., Krotenko, N.M., Fattakhov, N.S., Bokhan, N.A., Semke, A.V., Ivanova, S.A., and Buneva, V.N. (2015). DNA-Hydrolysing Activity of IgG Antibodies from the Sera of Patients with Schizophrenia. Open Biol., 5.","DOI":"10.1098\/rsob.150064"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Ermakov, E.A., Parshukova, D.A., Nevinsky, G.A., and Buneva, V.N. (2020). Natural Catalytic IgGs Hydrolyzing Histones in Schizophrenia: Are They the Link between Humoral Immunity and Inflammation?. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21197238"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nrrheum.2015.151","article-title":"Anti-DNA Antibodies\u2014Quintessential Biomarkers of SLE","volume":"12","author":"Pisetsky","year":"2016","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5816","DOI":"10.1111\/jcmm.13850","article-title":"Changes in Cell Differentiation and Proliferation Lead to Production of Abzymes in EAE Mice Treated with DNA\u2013Histone Complexes","volume":"22","author":"Aulova","year":"2018","journal-title":"J. Cell. Mol. Med."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Richter, P., Macovei, L.A., Mihai, I.R., Cardoneanu, A., Burlui, M.A., and Rezus, E. (2023). Cytokines in Systemic Lupus Erythematosus\u2014Focus on TNF-\u03b1 and IL-17. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914413"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.2217\/bmm-2019-0034","article-title":"Circulating Antioxidant Levels in Systemic Lupus Erythematosus Patients: A Systematic Review and Meta-Analysis","volume":"13","author":"Sam","year":"2019","journal-title":"Biomark. Med."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1126\/science.2727702","article-title":"Catalytic Hydrolysis of Vasoactive Intestinal Peptide by Human Autoantibody","volume":"244","author":"Paul","year":"1989","journal-title":"Science"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1052","DOI":"10.1093\/rheumatology\/kem112","article-title":"Fifty Years of Anti-Ds DNA Antibodies: Are We Approaching Journey\u2019s End?","volume":"46","author":"Isenberg","year":"2007","journal-title":"Rheumatology"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1002\/art.38365","article-title":"Review: Antinucleosome Antibodies: A Critical Reflection on Their Specificities and Diagnostic Impact","volume":"66","author":"Rekvig","year":"2014","journal-title":"Arthritis Rheumatol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1038\/nrrheum.2015.69","article-title":"The Anti-DNA Antibody: Origin and Impact, Dogmas and Controversies","volume":"11","author":"Rekvig","year":"2015","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Rekvig, O.P. (2022). The Anti-DNA Antibodies: Their Specificities for Unique DNA Structures and Their Unresolved Clinical Impact\u2014A System Criticism and a Hypothesis. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.808008"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1038\/nm.4027","article-title":"Neutrophil Extracellular Traps Enriched in Oxidized Mitochondrial DNA Are Interferogenic and Contribute to Lupus-like Disease","volume":"22","author":"Lood","year":"2016","journal-title":"Nat. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1002\/art.39417","article-title":"Circulating Apoptotic Microparticles in Systemic Lupus Erythematosus Patients Drive the Activation of Dendritic Cell Subsets and Prime Neutrophils for NETosis","volume":"68","author":"Dieker","year":"2016","journal-title":"Arthritis Rheumatol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1084\/jem.20081165","article-title":"Induction of Inflammatory and Immune Responses by HMGB1\u2013Nucleosome Complexes: Implications for the Pathogenesis of SLE","volume":"205","author":"Urbonaviciute","year":"2008","journal-title":"J. Exp. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1056\/NEJM198302243080802","article-title":"Polyspecificity of Monoclonal Lupus Autoantibodies Produced by Human-Human Hybridomas","volume":"308","author":"Shoenfeld","year":"1983","journal-title":"N. Engl. J. Med."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.jaut.2009.03.011","article-title":"Polyreactive Autoantibodies in Systemic Lupus Erythematosus Have Pathogenic Potential","volume":"33","author":"Zhang","year":"2009","journal-title":"J. Autoimmun."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1038\/nm1101-1189","article-title":"A Subset of Lupus Anti-DNA Antibodies Cross-Reacts with the NR2 Glutamate Receptor in Systemic Lupus Erythematosus","volume":"7","author":"DeGiorgio","year":"2001","journal-title":"Nat. Med."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1038\/s41467-023-37083-x","article-title":"Affinity Maturation Generates Pathogenic Antibodies with Dual Reactivity to DNase1L3 and dsDNA in Systemic Lupus Erythematosus","volume":"14","author":"Yu","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e20201138","DOI":"10.1084\/jem.20201138","article-title":"Autoantibody-Mediated Impairment of DNASE1L3 Activity in Sporadic Systemic Lupus Erythematosus","volume":"218","author":"Hartl","year":"2021","journal-title":"J. Exp. Med."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Nevinsky, G.A., Buneva, V.N., and Dmitrienok, P.S. (2022). Autoimmune Diseases: Enzymatic Cross Recognition and Hydrolysis of H2B Histone, Myelin Basic Protein, and DNA by IgGs against These Antigens. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23158102"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.molimm.2015.11.001","article-title":"Anti-DNA Antibody Mediated Catalysis Is Isotype Dependent","volume":"69","author":"Xia","year":"2016","journal-title":"Mol. Immunol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1080\/03009740701772465","article-title":"Increased Levels of Autoantibodies against Catalase and Superoxide Dismutase Associated with Oxidative Stress in Patients with Rheumatoid Arthritis and Systemic Lupus Erythematosus","volume":"37","author":"Mansour","year":"2008","journal-title":"Scand. J. Rheumatol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mito.2016.05.007","article-title":"The Pathogenesis of Systemic Lupus Erythematosus\u2014From the Viewpoint of Oxidative Stress and Mitochondrial Dysfunction","volume":"30","author":"Lee","year":"2016","journal-title":"Mitochondrion"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/nrrheum.2013.147","article-title":"Oxidative Stress in the Pathology and Treatment of Systemic Lupus Erythematosus","volume":"9","author":"Perl","year":"2013","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1756-185X.2011.01630.x","article-title":"Oxidative Stress in Systemic Lupus Erythematosus and Rheumatoid Arthritis Patients: Relationship to Disease Manifestations and Activity: Oxidative Stress in SLE and RA Patients","volume":"14","author":"Hassan","year":"2011","journal-title":"Int. J. Rheum. Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00018-023-04853-1","article-title":"Catalytic Antibodies in Arrhythmogenic Cardiomyopathy Patients Cleave Desmoglein 2 and N-Cadherin and Impair Cardiomyocyte Cohesion","volume":"80","author":"Yeruva","year":"2023","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1136\/annrheumdis-2018-214819","article-title":"2019 European League Against Rheumatism\/American College of Rheumatology Classification Criteria for Systemic Lupus Erythematosus","volume":"78","author":"Aringer","year":"2019","journal-title":"Ann. Rheum. Dis."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"2550","DOI":"10.1056\/NEJMoa051135","article-title":"Combined Oral Contraceptives in Women with Systemic Lupus Erythematosus","volume":"353","author":"Petri","year":"2005","journal-title":"N. Engl. J. Med."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0076-6879(84)05016-3","article-title":"[13] Catalase in Vitro","volume":"Volume 105","author":"Aebi","year":"1984","journal-title":"Methods in Enzymology"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0021-9258(19)50881-X","article-title":"A Spectrophotometric Method for Measuring the Breakdown of Hydrogen Peroxide by Catalase","volume":"195","author":"Beers","year":"1952","journal-title":"J. Biol. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/19\/9635\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:20:19Z","timestamp":1759422019000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/19\/9635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,2]]},"references-count":87,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["ijms26199635"],"URL":"https:\/\/doi.org\/10.3390\/ijms26199635","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,2]]}}}