{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T10:16:36Z","timestamp":1789035396063,"version":"build-2803163510"},"reference-count":39,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2021YFA0910800"],"award-info":[{"award-number":["2021YFA0910800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["SZYJY2022005"],"award-info":[{"award-number":["SZYJY2022005"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["AML2023B02"],"award-info":[{"award-number":["AML2023B02"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"HZAU-AGIS Cooperation Fund","award":["2021YFA0910800"],"award-info":[{"award-number":["2021YFA0910800"]}]},{"name":"HZAU-AGIS Cooperation Fund","award":["SZYJY2022005"],"award-info":[{"award-number":["SZYJY2022005"]}]},{"name":"HZAU-AGIS Cooperation Fund","award":["AML2023B02"],"award-info":[{"award-number":["AML2023B02"]}]},{"name":"National Key Laboratory of Agricultural Microbiology","award":["2021YFA0910800"],"award-info":[{"award-number":["2021YFA0910800"]}]},{"name":"National Key Laboratory of Agricultural Microbiology","award":["SZYJY2022005"],"award-info":[{"award-number":["SZYJY2022005"]}]},{"name":"National Key Laboratory of Agricultural Microbiology","award":["AML2023B02"],"award-info":[{"award-number":["AML2023B02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Bacterial microcompartments (BMCs) are intracellular structures for compartmentalizing specific metabolic pathways in bacteria. As a unique type of BMCs, carboxysomes utilize protein shells to sequester ribulose-1,5-bisphosphate carboxylase\/oxygenase (Rubisco) and carbonic anhydrase for efficient carbon dioxide (CO2) fixation. This study aims to reconstruct an \u03b1-carboxysome in Saccharomyces cerevisiae and investigate its metabolic effects. Here, genes of the cso operon from Halothiobacillus neapolitanus, Calvin cycle-related enzyme phosphoribulokinase (PRK) from Spinacia oleracea, and two S. cerevisiae chaperone genes, HSP60 and HSP10, were introduced into S. cerevisiae. The engineered yeast strain demonstrated assembled and enzymatically active Rubisco, significant increase in ethanol production and reduction in the byproduct glycerol. Formation of the \u03b1-carboxysome structures was observed after purification by sucrose density gradient centrifugation. The engineered yeast strain harboring functional \u03b1-carboxysome has the potential for enhancing bioethanol production.<\/jats:p>","DOI":"10.3390\/ijms26199759","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T15:48:30Z","timestamp":1759852110000},"page":"9759","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Engineering CO2-Fixing Carboxysome into Saccharomyces cerevisiae to Improve Ethanol Production"],"prefix":"10.3390","volume":"26","author":[{"given":"Mengqi","family":"Li","sequence":"first","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simin","family":"Zeng","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunling","family":"Guo","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Ji","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuling","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9365-362X","authenticated-orcid":false,"given":"Deqiang","family":"Duanmu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China"},{"name":"Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518108, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1002\/cbic.201600431","article-title":"A catalytic nanoreactor based on in vivo encapsulation of multiple enzymes in an engineered protein nanocompartment","volume":"17","author":"Giessen","year":"2016","journal-title":"ChemBioChem"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nchembio.2429","article-title":"Harnessing yeast organelles for metabolic engineering","volume":"13","author":"Hammer","year":"2017","journal-title":"Nat. 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