{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T07:44:13Z","timestamp":1776411853625,"version":"3.51.2"},"reference-count":37,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education (MOE) and the Daegu, Republic of Korea","award":["2025-RISE-03-001"],"award-info":[{"award-number":["2025-RISE-03-001"]}]},{"name":"Ministry of Education (MOE) and the Daegu, Republic of Korea","award":["RS-2025-02214096"],"award-info":[{"award-number":["RS-2025-02214096"]}]},{"name":"Rural Development Administration, Republic of Korea","award":["2025-RISE-03-001"],"award-info":[{"award-number":["2025-RISE-03-001"]}]},{"name":"Rural Development Administration, Republic of Korea","award":["RS-2025-02214096"],"award-info":[{"award-number":["RS-2025-02214096"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Damping-off disease hinders rice seedling growth and reduces yield. Current control methods, such as seed or soil sterilization, rely on chemicals that cause environmental pollution and promote pathogen resistance. As a sustainable alternative, we targeted the damping-off resistance-related gene OsDGTq1 using CRISPR\/Cas9. Field experiments first verified OsDGTq1\u2019s significance in resistance. The CRISPR\/Cas9 system, delivered via Agrobacterium-mediated transformation, was used to edit OsDGTq1 in rice cultivar Ilmi. Lesions from major damping-off pathogens, Rhizoctonia solani and Pythium graminicola, were observed on G0 plants. All 37 regenerated plants contained T-DNA insertions. Among them, edits generated by sgRNA1-1, sgRNA1-2, and sgRNA1-3 resulted in the insertion of two thymine bases as target mutations. Edited lines were assigned names and evaluated for agronomic traits, seed-setting rates, and pathogen responses. Several lines with edited target genes showed distinct disease responses and altered gene expression compared to Ilmi, likely due to CRISPR\/Cas9-induced sequence changes. Further studies in subsequent generations are needed to confirm the stability of these edits and their association with resistance. These results confirm that genome editing of OsDGTq1 alters resistance to damping-off. The approach demonstrates that gene-editing technology can accelerate rice breeding, offering an environmentally friendly strategy to develop resistant varieties. Such varieties can reduce chemical inputs, prevent pollution, and minimize seedling loss, ultimately enhancing food self-sufficiency and stabilizing rice supply.<\/jats:p>","DOI":"10.3390\/ijms26199761","type":"journal-article","created":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T15:48:30Z","timestamp":1759852110000},"page":"9761","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of Resistance to Damping-Off in Rice, Oryza sativa L., Using CRISPR\/Cas9"],"prefix":"10.3390","volume":"26","author":[{"given":"Seung-Kyo","family":"Jeong","sequence":"first","affiliation":[{"name":"Field Crop Research Division, National Institute of Crop and Food Science, RDA, Miryang 50424, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2207-5313","authenticated-orcid":false,"given":"Jae-Ryoung","family":"Park","sequence":"additional","affiliation":[{"name":"Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun-Gyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4812-6297","authenticated-orcid":false,"given":"Kyung-Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Coastal Agriculture Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea"},{"name":"Department of Applied Biosciences, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100491","DOI":"10.1016\/j.gfs.2021.100491","article-title":"Inclusiveness of consumer access to food safety: Evidence from certified rice in Vietnam","volume":"28","author":"My","year":"2021","journal-title":"Glob. 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