{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:11:05Z","timestamp":1760832665448,"version":"build-2065373602"},"reference-count":16,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T00:00:00Z","timestamp":1760572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We investigated the diagnostic efficacy of the universal transport medium\u2122 (UTM\u00ae) as a transport medium for aqueous humor polymerase chain reaction (PCR) testing in patients with clinically suspected viral uveitis. This retrospective study included 31 patients (31 eyes) with presumed viral uveitis who underwent anterior chamber sampling and compared the viral detection rates between using UTM\u00ae and conventional test tubes only. The positivity rate for any virus, including cytomegalovirus, herpes simplex virus, and varicella zoster virus, was significantly higher in the UTMT\u00ae group than in the test tube group (64.3% vs. 23.5%, p = 0.033). Logistic regression analysis also revealed that the use of UTM\u00ae significantly increased the PCR positivity rate (odds ratio, 5.850; 95% confidence interval, 1.222\u201327.994; p = 0.027). Thus, the use of UTM\u00ae was associated with improved detection of causative pathogens in patients with presumed viral uveitis.<\/jats:p>","DOI":"10.3390\/ijms262010091","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T16:33:22Z","timestamp":1760632402000},"page":"10091","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic Performance of Universal Transport Medium for Viral Polymerase Chain Reaction in Aqueous Humor Samples of Suspected Viral Uveitis: A Pilot Methods Study"],"prefix":"10.3390","volume":"26","author":[{"given":"Chungwoon","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6871-6721","authenticated-orcid":false,"given":"Yoo-Ri","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2860-2340","authenticated-orcid":false,"given":"Ji Hun","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8129-1174","authenticated-orcid":false,"given":"Young Joon","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3226-6148","authenticated-orcid":false,"given":"Hae Rang","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1080\/09273948.2023.2271077","article-title":"Viral anterior uveitis: A practical and comprehensive review of diagnosis and treatment","volume":"32","author":"Feng","year":"2024","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.ophtha.2023.12.034","article-title":"Use of anterior chamber paracentesis for diagnosis in viral anterior uveitis","volume":"131","author":"Chen","year":"2024","journal-title":"Ophthalmology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Standardization of Uveitis Nomenclature (SUN) Working Group (2021). Classification criteria for varicella zoster virus anterior uveitis. Am. J. Ophthalmol., 228, 165\u2013173.","DOI":"10.1016\/j.ajo.2021.03.037"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Standardization of Uveitis Nomenclature (SUN) Working Group (2021). Classification criteria for herpes simplex virus anterior uveitis. Am. J. Ophthalmol., 228, 231\u2013236.","DOI":"10.1016\/j.ajo.2021.03.053"},{"key":"ref_5","unstructured":"Standardization of Uveitis Nomenclature (SUN) Working Group (2021). Classification criteria for cytomegalovirus anterior uveitis. 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