{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T06:20:11Z","timestamp":1785478811631,"version":"3.56.0"},"reference-count":70,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003762","name":"Institut Pasteur Paris through Pasteur International Concerted Action program","doi-asserted-by":"publisher","award":["ACIP2015-10"],"award-info":[{"award-number":["ACIP2015-10"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003762","name":"Innovation Program Flash Maturation","doi-asserted-by":"publisher","award":["INNOV-80-20"],"award-info":[{"award-number":["INNOV-80-20"]}],"id":[{"id":"10.13039\/501100003762","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Odyssey Reinsurance Company"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Gastric cancer (GC) often presents a poor prognosis due to its asymptomatic phenotype at early stages. Upper endoscopy, which is the current gold standard to diagnose GC, is invasive with limited sensitivity for detecting gastric preneoplasia. Non-invasive biomarkers, such as blood circulating proteins, offer a promising alternative for the early detection of gastric lesions. In this prospective study, we identified plasma protein biomarkers for gastric preneoplasia and cancer using mass spectrometry-based proteomics in an exploratory cohort (n = 39). Fifteen promising protein candidates emerged to distinguish patient categories and were further confirmed by enzyme-linked immunosorbent assays (ELISA) in plasma samples from a validation cohort of 138 participants. Our predictive models demonstrated high classification performance with a minimal set of biomarkers. A four-protein panel (ARG1, CA2, F13A1, S100A12) achieved 94.1\u201398.2% AUROC (95% CI) for distinguishing cancer from non-cancer cases, while a five-protein panel (ARG1, CA2, HPT, MAN2A1, LBP) reached 97.3\u201399.5% AUROC (95% CI) for distinguishing cancer or preneoplasia from healthy or non-atrophic gastritis cases on the full cohort. Leveraging simple blood sampling, this strategy holds promise to detect high-risk gastric lesions, even at asymptomatic stages. Such an approach could significantly improve early detection and clinical management of GC, offering direct benefit for patients.<\/jats:p>","DOI":"10.3390\/ijms262010114","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:07:22Z","timestamp":1760706442000},"page":"10114","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plasma Protein Biomarkers to Detect Early Gastric Preneoplasia and Cancer: A Prospective Study"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6815-2498","authenticated-orcid":false,"given":"Quentin","family":"Giai Gianetto","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, UAR CNRS 2024, Proteomics Core Facility, MSBio, 75015 Paris, France"},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Bioinformatics and Biostatistics Hub, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Michel","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 6047, Infection, Genotoxicity and Cancer Team, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8627-6726","authenticated-orcid":false,"given":"Thibaut","family":"Douch\u00e9","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, UAR CNRS 2024, Proteomics Core Facility, MSBio, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4284-9991","authenticated-orcid":false,"given":"Karine","family":"Nozeret","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 6047, Infection, Genotoxicity and Cancer Team, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aziz","family":"Zaanan","sequence":"additional","affiliation":[{"name":"Assitance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Cit\u00e9, Georges Pompidou European Hospital, Department of Gastroenterology and Digestive Oncology, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oriane","family":"Colussi","sequence":"additional","affiliation":[{"name":"Assitance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Cit\u00e9, Georges Pompidou European Hospital, Department of Gastroenterology and Digestive Oncology, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Trouilloud","sequence":"additional","affiliation":[{"name":"Assitance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Cit\u00e9, Georges Pompidou European Hospital, Department of Gastroenterology and Digestive Oncology, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Pernot","sequence":"additional","affiliation":[{"name":"Assitance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Cit\u00e9, Georges Pompidou European Hospital, Department of Gastroenterology and Digestive Oncology, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Noelle","family":"Ungeheuer","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Center for Translational Research, Clinical Investigation and Access to Clinical Resources (ICAReB), 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Juli\u00e9","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris, CHU Paris IdF Ouest, Pathology Unit Ambroise Par\u00e9 Hospital, 92100 Boulogne Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Jolly","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, Clinical Research Coordination Office, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9955-4753","authenticated-orcid":false,"given":"Julien","family":"Ta\u00efeb","sequence":"additional","affiliation":[{"name":"Assitance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Cit\u00e9, Georges Pompidou European Hospital, Department of Gastroenterology and Digestive Oncology, 75015 Paris, France"},{"name":"Universit\u00e9 Paris-Cit\u00e9, Department of Gastroenterology and Digestive Oncology, Georges Pompidou European Hospital, SIRIC CARPEM, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Lamarque","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris CHU Paris IdF Ouest, Unit of Hepato-Gastroenterology, Ambroise Par\u00e9 Hospital, 92100 Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3958-7710","authenticated-orcid":false,"given":"Mariette","family":"Matondo","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, UAR CNRS 2024, Proteomics Core Facility, MSBio, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3450-1773","authenticated-orcid":false,"given":"Eliette","family":"Touati","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR 6047, Infection, Genotoxicity and Cancer Team, 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"ref_1","first-page":"394","article-title":"Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mukaisho, K.-I., Nakayama, T., Hagiwara, T., Hattori, T., and Sugihara, H. (2015). Two Distinct Etiologies of Gastric Cardia Adenocarcinoma: Interactions among pH, Helicobacter Pylori, and Bile Acids. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00412"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jcmgh.2016.12.001","article-title":"The Clinical Evidence Linking Helicobacter Pylori to Gastric Cancer","volume":"3","author":"Moss","year":"2017","journal-title":"Cell Mol. Gastroenterol. Hepatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1136\/gutjnl-2019-320234","article-title":"Is Gastric Cancer Becoming a Rare Disease? A Global Assessment of Predicted Incidence Trends to 2035","volume":"69","author":"Arnold","year":"2020","journal-title":"Gut"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1093\/jnci\/djx262","article-title":"The Changing Face of Noncardia Gastric Cancer Incidence Among US Non-Hispanic Whites","volume":"110","author":"Anderson","year":"2018","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1053\/j.gastro.2023.11.286","article-title":"The Effect of Nationwide Organized Cancer Screening Programs on Gastric Cancer Mortality: A Synthetic Control Study","volume":"166","author":"Sun","year":"2024","journal-title":"Gastroenterology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e535","DOI":"10.1016\/S1470-2045(13)70436-4","article-title":"Management of Gastric Cancer in Asia: Resource-Stratified Guidelines","volume":"14","author":"Shen","year":"2013","journal-title":"Lancet Oncol."},{"key":"ref_8","first-page":"6735","article-title":"Human Gastric Carcinogenesis: A Multistep and Multifactorial Process--First American Cancer Society Award Lecture on Cancer Epidemiology and Prevention","volume":"52","author":"Correa","year":"1992","journal-title":"Cancer Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1016\/j.cgh.2017.05.023","article-title":"Gastric Cancer as Preventable Disease","volume":"15","author":"Rugge","year":"2017","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1136\/gutjnl-2024-333684","article-title":"Commentary on: The Management of Patients with Gastric Intestinal Metaplasia","volume":"74","author":"Graham","year":"2024","journal-title":"Gut"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2570","DOI":"10.1016\/S0140-6736(24)01171-1","article-title":"40 Years after the Discovery of Helicobacter Pylori: Towards Elimination of H Pylori for Gastric Cancer Prevention","volume":"403","author":"Liou","year":"2024","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1053\/j.gastro.2022.05.027","article-title":"Long-Term Impact of Helicobacter Pylori Eradication Therapy on Gastric Cancer Incidence and Mortality in Healthy Infected Individuals: A Meta-Analysis Beyond 10 Years of Follow-Up","volume":"163","author":"Ford","year":"2022","journal-title":"Gastroenterology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1055\/a-0859-1883","article-title":"Management of Epithelial Precancerous Conditions and Lesions in the Stomach (MAPS II): European Society of Gastrointestinal Endoscopy (ESGE), European Helicobacter and Microbiota Study Group (EHMSG), European Society of Pathology (ESP), and Sociedade Portuguesa de Endoscopia Digestiva (SPED) Guideline Update 2019","volume":"51","author":"Areia","year":"2019","journal-title":"Endoscopy"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1136\/gutjnl-2024-333029","article-title":"The Road to a World-Unified Approach to the Management of Patients with Gastric Intestinal Metaplasia: A Review of Current Guidelines","volume":"73","author":"Shah","year":"2024","journal-title":"Gut"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Beck, M., Bringeland, E.A., Qvigstad, G., and Fossmark, R. (2021). Gastric Cancers Missed at Upper Endoscopy in Central Norway 2007 to 2016-A Population-Based Study. Cancers, 13.","DOI":"10.3390\/cancers13225628"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1126\/science.aar3247","article-title":"Detection and Localization of Surgically Resectable Cancers with a Multi-Analyte Blood Test","volume":"359","author":"Cohen","year":"2018","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4","DOI":"10.14740\/wjon1425","article-title":"Carcinoembryonic Antigen, Carbohydrate Antigen 19-9, Cancer Antigen 125, Prostate-Specific Antigen and Other Cancer Markers: A Primer on Commonly Used Cancer Markers","volume":"14","author":"Desai","year":"2023","journal-title":"World J. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Feng, F., Tian, Y., Xu, G., Liu, Z., Liu, S., Zheng, G., Guo, M., Lian, X., Fan, D., and Zhang, H. (2017). Diagnostic and Prognostic Value of CEA, CA19-9, AFP and CA125 for Early Gastric Cancer. BMC Cancer, 17.","DOI":"10.1186\/s12885-017-3738-y"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"136","DOI":"10.3109\/00365521.2011.645501","article-title":"Rationale in Diagnosis and Screening of Atrophic Gastritis with Stomach-Specific Plasma Biomarkers","volume":"47","author":"Kuipers","year":"2012","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"460","DOI":"10.3892\/br.2017.985","article-title":"Serum Pepsinogen and Gastrin-17 as Potential Biomarkers for Pre-Malignant Lesions in the Gastric Corpus","volume":"7","author":"Loong","year":"2017","journal-title":"Biomed. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ebiom.2019.05.044","article-title":"A Targeted Proteomics Approach Reveals a Serum Protein Signature as Diagnostic Biomarker for Resectable Gastric Cancer","volume":"44","author":"Shen","year":"2019","journal-title":"EBioMedicine"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Bazin, T., Nozeret, K., Juli\u00e9, C., Lamarque, D., and Touati, E. (2024). Protein Biomarkers of Gastric Preneoplasia and Cancer Lesions in Blood: A Comprehensive Review. Cancers, 16.","DOI":"10.20944\/preprints202407.2470.v1"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-Based Map of the Human Proteome","volume":"347","author":"Fagerberg","year":"2015","journal-title":"Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1111\/j.1523-5378.2009.00728.x","article-title":"Serum Levels of Leptin as Marker for Patients at High Risk of Gastric Cancer","volume":"14","author":"Capelle","year":"2009","journal-title":"Helicobacter"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1684\/ecn.2018.0408","article-title":"Clinical Significance of Serum Leptin Level in Patients with Gastric Cancer","volume":"29","author":"Tas","year":"2018","journal-title":"Eur. Cytokine Netw."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5126","DOI":"10.3748\/wjg.v27.i31.5126","article-title":"Endoscopic Diagnosis and Treatment of Gastric Dysplasia and Early Cancer: Current Evidence and What the Future May Hold","volume":"27","author":"Young","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kim, Y.-I., Park, J.Y., Kim, B.J., Hwang, H.W., Hong, S.A., and Kim, J.G. (2020). Risk of Metachronous Gastric Neoplasm Occurrence during Intermediate-Term Follow-up Period after Endoscopic Submucosal Dissection for Gastric Dysplasia. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-63722-0"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"105262","DOI":"10.1016\/j.jprot.2024.105262","article-title":"Delineating Protein Biomarkers for Gastric Cancers: A Catalogue of Mass Spectrometry-Based Markers and Assessment of Their Suitability for Targeted Proteomics Applications","volume":"306","author":"Ramesh","year":"2024","journal-title":"J. Proteom."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kim, M.-H., and Kim, H. (2021). Role of Leptin in the Digestive System. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.660040"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Niu, F., Yu, Y., Li, Z., Ren, Y., Li, Z., Ye, Q., Liu, P., Ji, C., Qian, L., and Xiong, Y. (2022). Arginase: An Emerging and Promising Therapeutic Target for Cancer Treatment. Biomed. Pharmacother., 149.","DOI":"10.1016\/j.biopha.2022.112840"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e19159","DOI":"10.1097\/MD.0000000000019159","article-title":"The Significance of Arginase-1 Expression in the Diagnosis of Liver Cancer: A Protocol for a Systematic Review","volume":"99","author":"Wang","year":"2020","journal-title":"Medicine"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s10863-012-9424-5","article-title":"ATAD3, a Vital Membrane Bound Mitochondrial ATPase Involved in Tumor Progression","volume":"44","author":"Li","year":"2012","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_33","first-page":"1304","article-title":"Prognostic Value of ATAD3 Gene Cluster Expression in Hepatocellular Carcinoma","volume":"18","author":"Liu","year":"2019","journal-title":"Oncol. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3748\/wjg.v11.i2.155","article-title":"Carbonic Anhydrases in Normal Gastrointestinal Tract and Gastrointestinal Tumours","volume":"11","author":"Saarnio","year":"2005","journal-title":"World J. Gastroenterol."},{"key":"ref_35","first-page":"6716","article-title":"Low CA II Expression Is Associated with Tumor Aggressiveness and Poor Prognosis in Gastric Cancer Patients","volume":"7","author":"Hu","year":"2014","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1080\/14789450.2020.1740091","article-title":"Glycosylation of Serum Haptoglobin as a Marker of Gastric Cancer: An Overview for Clinicians","volume":"17","author":"Jeong","year":"2020","journal-title":"Expert. Rev. Proteom."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1038\/bjc.2017.96","article-title":"Serum Inflammatory Markers and Colorectal Cancer Risk and Survival","volume":"116","author":"Ghuman","year":"2017","journal-title":"Br. J. Cancer"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"113414","DOI":"10.1016\/j.intimp.2024.113414","article-title":"IGFALS Suppresses Hepatocellular Carcinoma Progression by Stabilizing PPAR-\u03b3","volume":"143","author":"Xu","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jare.2020.06.026","article-title":"Effects of Differential Distributed-JUP on the Malignancy of Gastric Cancer","volume":"28","author":"Chen","year":"2021","journal-title":"J. Adv. Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"62159","DOI":"10.18632\/oncotarget.11398","article-title":"PADI4 Has Genetic Susceptibility to Gastric Carcinoma and Upregulates CXCR2, KRT14 and TNF-\u03b1 Expression Levels","volume":"7","author":"Zheng","year":"2016","journal-title":"Oncotarget"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Lv, H., Lv, M., Guo, X., Zhu, X., Chao, Y., and Li, D. (2025). Lipopolysaccharide-Binding Protein (LBP): A Prognostic Biomarker for Gastric Cancer Linked to Immune Infiltration. BMC Gastroenterol., 25.","DOI":"10.1186\/s12876-025-03794-2"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1186\/s13046-023-02833-8","article-title":"Gastric Cancer-Derived LBP Promotes Liver Metastasis by Driving Intrahepatic Fibrotic Pre-Metastatic Niche Formation","volume":"42","author":"Xie","year":"2023","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3686","DOI":"10.21037\/tcr-22-629","article-title":"MAN2A1 Predicts Prognosis and Progression through Cancer-Related Pathways in Colorectal Cancer","volume":"11","author":"Wang","year":"2022","journal-title":"Transl. Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6551","DOI":"10.1007\/s13277-015-4460-5","article-title":"Expression and Clinical Implication of S100A12 in Gastric Carcinoma","volume":"37","author":"Li","year":"2016","journal-title":"Tumour Biol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Ercan, H., Mauracher, L.-M., Grilz, E., Hell, L., Hellinger, R., Schmid, J.A., Moik, F., Ay, C., Pabinger, I., and Zellner, M. (2021). Alterations of the Platelet Proteome in Lung Cancer: Accelerated F13A1 and ER Processing as New Actors in Hypercoagulability. Cancers, 13.","DOI":"10.3390\/cancers13092260"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.3349\/ymj.2013.54.6.1394","article-title":"Relationships of Coagulation Factor XIII Activity with Cell-Type and Stage of Non-Small Cell Lung Cancer","volume":"54","author":"Lee","year":"2013","journal-title":"Yonsei Med. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1097\/PAI.0000000000000404","article-title":"Factor XIII Subunit A Immunohistochemical Expression Is Associated With Inferior Outcomes in Acute Promyelocytic Leukemia","volume":"26","author":"Raval","year":"2018","journal-title":"Appl. Immunohistochem. Mol. Morphol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Liu, Y., Deng, H., Song, P., and Zhang, M. (2025). Constructing a Glioblastoma Prognostic Model Related to Fatty Acid Metabolism Using Machine Learning and Identifying F13A1 as a Potential Target. Biomedicines, 13.","DOI":"10.3390\/biomedicines13020256"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"103920","DOI":"10.1016\/j.autrev.2025.103920","article-title":"The Roles of S100A8\/A9 and S100A12 in Autoimmune Diseases: Mechanisms, Biomarkers, and Therapeutic Potential","volume":"24","author":"Wang","year":"2025","journal-title":"Autoimmun. Rev."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"4580","DOI":"10.1046\/j.1432-1327.2001.02385.x","article-title":"Essential Roles of CD14 and Lipopolysaccharide-Binding Protein for Activation of Toll-like Receptor (TLR)2 as Well as TLR4 Reconstitution of TLR2- and TLR4-Activation by Distinguishable Ligands in LPS Preparations","volume":"268","author":"Muta","year":"2001","journal-title":"Eur. J. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1038\/modpathol.2009.189","article-title":"Carbonic Anhydrase II. A Novel Biomarker for Gastrointestinal Stromal Tumors","volume":"23","author":"Parkkila","year":"2010","journal-title":"Mod. Pathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5990","DOI":"10.1158\/1078-0432.CCR-20-0778","article-title":"Inhibition of MAN2A1 Enhances the Immune Response to Anti-PD-L1 in Human Tumors","volume":"26","author":"Shi","year":"2020","journal-title":"Clin. Cancer Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1600-065X.2010.00915.x","article-title":"Control of Immune Response by Amino Acid Metabolism","volume":"236","author":"Grohmann","year":"2010","journal-title":"Immunol. Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"112766","DOI":"10.1016\/j.jep.2020.112766","article-title":"Lily Steroidal Glycoalkaloid Promotes Early Inflammatory Resolution in Wounded Human Fibroblasts","volume":"258","author":"Di","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.2337\/db21-0842","article-title":"Leptin Signaling Suppression in Macrophages Improves Immunometabolic Outcomes in Obesity","volume":"71","author":"Monteiro","year":"2022","journal-title":"Diabetes"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1042\/BJ20071137","article-title":"Contribution of Leptin Receptor N-Linked Glycans to Leptin Binding","volume":"410","author":"Kamikubo","year":"2008","journal-title":"Biochem. J."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1055\/a-2529-5025","article-title":"Management of Epithelial Precancerous Conditions and Early Neoplasia of the Stomach (MAPS III): European Society of Gastrointestinal Endoscopy (ESGE), European Helicobacter and Microbiota Study Group (EHMSG) and European Society of Pathology (ESP) Guideline Update 2025","volume":"57","author":"Uchima","year":"2025","journal-title":"Endoscopy"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1093\/bioinformatics\/btl117","article-title":"Pvclust: An R Package for Assessing the Uncertainty in Hierarchical Clustering","volume":"22","author":"Suzuki","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Gianetto, Q.G., Wieczorek, S., Cout\u00e9, Y., and Burger, T. (2020). A Peptide-Level Multiple Imputation Strategy Accounting for the Different Natures of Missing Values in Proteomics Data. bioRxiv.","DOI":"10.1101\/2020.05.29.122770"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Rohart, F., Gautier, B., Singh, A., and L\u00ea Cao, K.-A. (2017). mixOmics: An R Package for \u2018omics Feature Selection and Multiple Data Integration. PLoS Comput. Biol., 13.","DOI":"10.1101\/108597"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/978-1-0716-1967-4_12","article-title":"Statistical Analysis of Post-Translational Modifications Quantified by Label-Free Proteomics Across Multiple Biological Conditions with R: Illustration from SARS-CoV-2 Infected Cells","volume":"Volume 2426","author":"Burger","year":"2023","journal-title":"Statistical Analysis of Proteomic Data"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"Limma Powers Differential Expression Analyses for RNA-Sequencing and Microarray Studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1002\/pmic.201500189","article-title":"Calibration Plot for Proteomics: A Graphical Tool to Visually Check the Assumptions Underlying FDR Control in Quantitative Experiments","volume":"16","author":"Combes","year":"2016","journal-title":"Proteomics"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1093\/bioinformatics\/btl328","article-title":"Robust Estimation of the False Discovery Rate","volume":"22","author":"Pounds","year":"2006","journal-title":"Bioinformatics"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Robin, X., Turck, N., Hainard, A., Tiberti, N., Lisacek, F., Sanchez, J.-C., and M\u00fcller, M. (2011). pROC: An Open-Source Package for R and S+ to Analyze and Compare ROC Curves. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-77"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"DeLong, E.R., DeLong, D.M., and Clarke-Pearson, D.L. (1988). Comparing the Areas under Two or More Correlated Receiver Operating Characteristic Curves: A Nonparametric Approach. Biometrics, 44.","DOI":"10.2307\/2531595"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1038\/nprot.2007.261","article-title":"Protocol for Micro-Purification, Enrichment, Pre-Fractionation and Storage of Peptides for Proteomics Using StageTips","volume":"2","author":"Rappsilber","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nmeth.2834","article-title":"Minimal, Encapsulated Proteomic-Sample Processing Applied to Copy-Number Estimation in Eukaryotic Cells","volume":"11","author":"Kulak","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1038\/nbt.1511","article-title":"MaxQuant Enables High Peptide Identification Rates, Individualized p.p.b.-Range Mass Accuracies and Proteome-Wide Protein Quantification","volume":"26","author":"Cox","year":"2008","journal-title":"Nat. Biotechnol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1021\/pr101065j","article-title":"Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment","volume":"10","author":"Cox","year":"2011","journal-title":"J. Proteome Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/20\/10114\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:46:27Z","timestamp":1760957187000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/20\/10114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,17]]},"references-count":70,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["ijms262010114"],"URL":"https:\/\/doi.org\/10.3390\/ijms262010114","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,17]]}}}