{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T10:33:55Z","timestamp":1776940435918,"version":"3.51.4"},"reference-count":61,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["FSSF-2023-0046"],"award-info":[{"award-number":["FSSF-2023-0046"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Pancreatic inflammation and subsequent fibrosis drive serious disease complications. However, the pathogenesis of this process and the mechanisms underlying excessive extracellular matrix (ECM) deposition remain poorly understood. Our aim was to study intercellular interactions and ultrastructural changes in mast cells, pancreatic stellate cells, and telocytes, as well as in the extracellular matrix in various degrees of pancreatic fibrosis. Histological, immunohistochemical, and electron microscopic (EM) studies were performed on surgical materials from 17 patients. Mapping of fibrosis fields was performed on scanned images using the QuPath software v0.6.0. The IHC study was performed using a panel of antibodies: CD34, CD117, and SMA. Fluorescent IHC was performed using a panel of antibodies: CD34 and CD117. The EM study was performed on ultrathin sections with a thickness of 100\u2013120 nm. The functional activity of mast cells (MCs) increased in pancreatic fibrosis. Most of the MCs were in a degranulation state, with the formation of intercellular contacts. The activation of pancreatic stellate cells (PaSCs), which underwent ultrastructural and functional changes in pancreatic fibrosis that developed as a result of chronic pancreatitis (CP), was noted. Multiple plasmolemma discontinuities, telopode shortenings, and nuclear fragmentations were observed among telocytes (TCs). The presence of MCs in the inflammatory infiltrate, as well as the destruction of TCs with the activation of exosomal transport, plays an important role in the pathogenesis of fibrosis in CP and provides a promising therapeutic target for the treatment of this pathology.<\/jats:p>","DOI":"10.3390\/ijms262010169","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T08:18:54Z","timestamp":1760948334000},"page":"10169","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mast Cells, Pancreatic Stellate Cells, and Telocytes in Chronic Pancreatitis: Ultrastructural Study"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0126-4473","authenticated-orcid":false,"given":"Irina","family":"Chekmareva","sequence":"first","affiliation":[{"name":"Federal State Budgetary Institution \u201cNational Medical Research Center of Surgery Named after A.V. Vishnevsky\u201d of the Ministry of Health of the Russian Federation, Bolshaya Serpukhovskaya St, 27, 115093 Moscow, Russia"},{"name":"Research Institute of Human Morphology Named After Academician A.P. Avtsyn, Petrovsky National Research Center of Surgery, 3 Tsyurupy St, 117418 Moscow, Russia"},{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrey","family":"Kostin","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oksana","family":"Paklina","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cNational Medical Research Center of Surgery Named after A.V. Vishnevsky\u201d of the Ministry of Health of the Russian Federation, Bolshaya Serpukhovskaya St, 27, 115093 Moscow, Russia"},{"name":"State Budgetary Healthcare Institution \u201cMoscow Clinical Scientific Center Named After A.S. Loginov, Department of Health of the City of Moscow\u201d, Enthusiasts Highway, 86, 111123 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6247-9481","authenticated-orcid":false,"given":"Dmitry","family":"Kalinin","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cNational Medical Research Center of Surgery Named after A.V. Vishnevsky\u201d of the Ministry of Health of the Russian Federation, Bolshaya Serpukhovskaya St, 27, 115093 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9435-7527","authenticated-orcid":false,"given":"Dmitry","family":"Suraev","sequence":"additional","affiliation":[{"name":"State Budgetary Healthcare Institution \u201cMoscow City Oncological Hospital No. 62, Department of Health of the City of Moscow\u201d, pos. Istra, 27, building 1 to 30, Krasnogorsk Municipal District, 143515 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0889-2720","authenticated-orcid":false,"given":"Nikolay","family":"Karnaukhov","sequence":"additional","affiliation":[{"name":"State Budgetary Healthcare Institution \u201cMoscow Clinical Scientific Center Named After A.S. Loginov, Department of Health of the City of Moscow\u201d, Enthusiasts Highway, 86, 111123 Moscow, Russia"},{"name":"Institute of Clinical Morphology and Digital Pathology, Sechenov University, Bolshaya Pirogovskaya Street, Bl. 2\/4, 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Alekhnovich","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1749-9563","authenticated-orcid":false,"given":"Atim","family":"Emaimo John","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9185-4578","authenticated-orcid":false,"given":"Viktoria","family":"Shishkina","sequence":"additional","affiliation":[{"name":"Research Institute of Experimental Biology and Medicine, Burdenko Voronezh State Medical University, 394036 Voronezh, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1142-7483","authenticated-orcid":false,"given":"Igor","family":"Buchwalow","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"},{"name":"Institute for Hematopathology, Fangdieckstr. 75a, 22547 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4060-8355","authenticated-orcid":false,"given":"Markus","family":"Tiemann","sequence":"additional","affiliation":[{"name":"Institute for Hematopathology, Fangdieckstr. 75a, 22547 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4556","authenticated-orcid":false,"given":"Dmitrii","family":"Atiakshin","sequence":"additional","affiliation":[{"name":"Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, RUDN University, 6 Miklukho-Maklaya St, 117198 Moscow, Russia"},{"name":"Research Institute of Experimental Biology and Medicine, Burdenko Voronezh State Medical University, 394036 Voronezh, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1007\/s10620-017-4621-z","article-title":"Validation of Demographics, Etiology, and Risk Factors for Chronic Pancreatitis in the USA: A Report of the North American Pancreas Study (NAPS) Group","volume":"62","author":"Conwell","year":"2017","journal-title":"Dig. 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