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No approved therapies currently halt the progression of this disease. Here, we evaluated the effects of daily oral administration of pulverized Boldo (Peumus boldus) leaves, commonly used as a nutraceutical, to blAJ mice, a model of dysferlinopathy. Symptomatic bIAJ mice were treated for four weeks with Boldo and presented significantly improved grip strength and restored endothelial-dependent vasodilation. Muscle perfusion and capillary density in the gastrocnemius were both enhanced by treatment. Histological analyses revealed that Boldo prevented myofiber atrophy, reduced centrally nucleated fibers, and improved muscle tissue architecture. Lipid accumulation observed in blAJ muscles was absent in Boldo-treated mice. At the cellular level, Boldo normalized sarcolemma membrane permeability (dye uptake) and reduced mRNA levels of inflammasome components (NLRP3, ASC, and IL-1\u03b2), suggesting anti-inflammatory activity. These findings indicate that Boldo improves vascular and muscle integrity, supporting its potential as a complementary therapeutic strategy for dysferlinopathy.<\/jats:p>","DOI":"10.3390\/ijms26209945","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T16:13:03Z","timestamp":1760717583000},"page":"9945","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Boldo Restores Vascularization and Reduces Skeletal Muscle Inflammation in Symptomatic Mice with Dysferlinopathy"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4113-8172","authenticated-orcid":false,"given":"Walter","family":"V\u00e1squez","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda, Pontificia Universidad Cat\u00f3lica de Chile, Santiago 8331150, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"Troncoso","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Vascular, Departamento de Ciencias, Universidad del Bio-Bio, Chill\u00e1n 3800708, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Lira","sequence":"additional","affiliation":[{"name":"Departamento de Morfolog\u00eda, Facultad de Medicina, Universidad Andres Bello, Santiago 8370211, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7688-4621","authenticated-orcid":false,"given":"Carlos","family":"Escudero","sequence":"additional","affiliation":[{"name":"Laboratorio de Fisiolog\u00eda Vascular, Departamento de Ciencias, Universidad del Bio-Bio, Chill\u00e1n 3800708, Chile"},{"name":"Group of Research and Innovation in Vascular Health, GRIVAS Health, Chill\u00e1n 3800708, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3811-0347","authenticated-orcid":false,"given":"Juan C.","family":"S\u00e1ez","sequence":"additional","affiliation":[{"name":"Instituto de Neurociencias, Centro Interdisciplinario de Neurociencias de Valpara\u00edso, Universidad de Valpara\u00edso, Valpara\u00edso 2360102, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nature01573","article-title":"Defective Membrane Repair in Dysferlin-Deficient Muscular Dystrophy","volume":"423","author":"Bansal","year":"2003","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1093\/hmg\/10.17.1761","article-title":"The Sarcolemmal Proteins Dysferlin and Caveolin-3 Interact in Skeletal Muscle","volume":"10","author":"Matsuda","year":"2001","journal-title":"Hum. 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