{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:59:09Z","timestamp":1792123149200,"version":"4.4.0"},"reference-count":51,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Polysaccharides from edible mushrooms are increasingly recognized as bioactive compounds with health-promoting properties. In this study, a polysaccharide-rich fraction Mp-CPS was isolated from fruiting bodies of Macrolepiota procera using ultrasound-assisted extraction. The chemical composition of crude polysaccharides from the parasol mushroom was evaluated using spectrophotometric and electrophoretic methods. Chemical analysis revealed that Mp-CPS is mainly composed of glucose- and galactose-based heteropolysaccharides, with \u03b2-glucans as the predominant glucan type. The biological potential of Mp-CPS was evaluated in light of its antioxidant, anti-inflammatory, and anticancer activities. Antioxidant assays (TEAC, ORAC) demonstrated significant radical-scavenging capacity, with higher activity observed in the ORAC test. As revealed by biochemical examination, Mp-CPS also inhibited key pro-inflammatory enzymes: COX-1, COX-2, and LOX. At the same time, in vitro research (MTT and LDH assays) has shown the great chemopreventive abilities of Mp-CPS against human colon cancer cells, which intensified with the degree of cell malignancy. Overall, these results highlight M. procera as a sustainable and valuable source of biologically active polysaccharides with antioxidant, anti-inflammatory, and anticancer potential. The findings support further exploration of Mp-CPS for applications in functional foods and nutraceuticals.<\/jats:p>","DOI":"10.3390\/ijms26209978","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T15:20:12Z","timestamp":1760973612000},"page":"9978","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chemical Characterization and In Vitro Antioxidant, Anti-Inflammatory, and Colon Cancer-Preventive Potential of a Polysaccharide Fraction from Macrolepiota procera"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1133-2150","authenticated-orcid":false,"given":"Natalia","family":"Nowacka-Jechalke","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Botany, Medical University of Lublin, Chod\u017aki 1, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9367-0684","authenticated-orcid":false,"given":"Marta Kinga","family":"Lemieszek","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108037","DOI":"10.1016\/j.carres.2020.108037","article-title":"Extraction methods, chemical characterizations and biological activities of mushroom polysaccharides: A mini-review","volume":"494","author":"Gong","year":"2020","journal-title":"Carbohydr. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhang, X., Duan, Y., Xue, J., Chen, S., and Wang, H. (2025). Edible mushroom polysaccharides: Structural characteristics, chemical modification strategies, and structure-activity relationship: A review. Int. J. Biol. Macromol., 320.","DOI":"10.1016\/j.ijbiomac.2025.145888"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"121978","DOI":"10.1016\/j.carbpol.2024.121978","article-title":"An overview on mushroom polysaccharides: Health-promoting properties, prebiotic and gut microbiota modulation effects and structure-function correlation","volume":"333","author":"Sousa","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.fshw.2021.04.001","article-title":"Immunomodulatory effects of polysaccharides from edible fungus: A review","volume":"10","author":"Yin","year":"2021","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"25887","DOI":"10.1021\/acsomega.4c00322","article-title":"Characterization of polysaccharide extracts of four edible mushrooms and determination of in vitro antioxidant, enzyme inhibition and anticancer activities","volume":"9","author":"Deveci","year":"2024","journal-title":"ACS Omega"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhong, X., Wang, G., Li, F., Fang, S., Zhou, S., Ishiwata, A., Tonevitsky, A.G., Shkurnikov, M., Cai, H., and Ding, F. (2023). Immunomodulatory effect and biological significance of \u03b2-glucans. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15061615"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Park, H.J. (2022). Current uses of mushrooms in cancer treatment and their anticancer mechanisms. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810502"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1002\/fft2.490","article-title":"From structure to function: A comprehensive overview of polysaccharide roles and applications","volume":"6","author":"Xu","year":"2025","journal-title":"Food Front."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Guo, D., Liu, C., Zhu, H., Cheng, Y., Guo, Y., Yao, W., Jiang, J., and Qian, H. (2025). Advanced insights into mushroom polysaccharides: Extraction methods, structure-activity, prebiotic properties, and health-promoting effects. Int. J. Biol. Macromol., 308.","DOI":"10.1016\/j.ijbiomac.2025.142319"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5773275","DOI":"10.1155\/2022\/5773275","article-title":"Possibilities of using Macrolepiota procera in the production of prohealth food and in medicine","volume":"2022","author":"Adamska","year":"2022","journal-title":"Int. J. Food Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Shim, S.M., Oh, Y.H., Lee, K.R., Kim, S.H., Im, K.H., Kim, J.W., Lee, U.Y., Shim, J.O., Shim, M.J., and Lee, M.W. (2005). The characteristics of cultural conditions for the mycelial growth of Macrolepiota procera. Mycobiology, 33.","DOI":"10.4489\/MYCO.2005.33.1.015"},{"key":"ref_12","first-page":"121","article-title":"Antioxidant and antimicrobial activities of Armillaria mellea and Macrolepiota procera extracts","volume":"11","author":"Aytar","year":"2020","journal-title":"J. Fungus"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"173274","DOI":"10.1155\/2010\/173274","article-title":"The role of polyphenols, \u03b2-carotene, and lycopene in the antioxidative action of the extracts of dried, edible mushrooms","volume":"2010","author":"Robaszkiewicz","year":"2010","journal-title":"J. Nutr. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Georgiev, Y.N., Vasicek, O., Dzhambazov, B., Batsalova, T.G., Denev, P.N., Dobreva, L.I., Danova, S.T., Simova, S.D., Wold, C.W., and Ognyanov, M.H. (2022). Structural features and immunomodulatory effects of water-extractable polysaccharides from Macrolepiota procera (Scop.) Singer. J. Fungi, 8.","DOI":"10.3390\/jof8080848"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1615\/IntJMedMushrooms.v17.i12.60","article-title":"Contents and antioxidant activities of polysaccharides in 14 wild mushroom species from the forest of northeastern China","volume":"17","author":"Xu","year":"2015","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1007\/s00394-017-1436-9","article-title":"The preliminary study of prebiotic potential of polish wild mushroom polysaccharides: The stimulation effect on Lactobacillus strains growth","volume":"57","author":"Nowak","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5794","DOI":"10.1039\/D2FO00680D","article-title":"Mycelium polysaccharides of Macrolepiota procera alleviate reproductive impairments induced by nonylphenol","volume":"13","author":"Wang","year":"2022","journal-title":"Food Funct."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.1016\/j.sjbs.2022.02.018","article-title":"Identification of Macrolepiota procera extract as a novel G6PD inhibitor for the treatment of lung cancer","volume":"29","author":"Zara","year":"2022","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1080\/02726351.2022.2089303","article-title":"Green synthesis of silver nanoparticles using Macrolepiota procera extract and investigation of their HSP27, HSP70, and HSP90 inhibitory potentials in human cancer cells","volume":"41","author":"Kaplan","year":"2023","journal-title":"Part. Sci. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nowacka-Jechalke, N., Nowak, R., Lemieszek, M.K., Rzeski, W., Gawlik-Dziki, U., Szpakowska, N., and Kaczy\u0144ski, Z. (2021). Promising potential of crude polysaccharides from Sparassis crispa against colon cancer: An in vitro study. Nutrients, 13.","DOI":"10.3390\/nu13010161"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/gutjnl-2022-327736","article-title":"Global burden of colorectal cancer in 2020 and 2040: Incidence and mortality estimates from GLOBOCAN","volume":"72","author":"Morgan","year":"2023","journal-title":"Gut"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.1038\/oncsis.2013.35","article-title":"Epigenetic and genetic features of 24 colon cancer cell lines","volume":"2","author":"Ahmed","year":"2013","journal-title":"Oncogenesis"},{"key":"ref_23","first-page":"3712","article-title":"1,25-dihydroxyvitamin D3 receptor as a marker of human colon carcinoma cell line differentiation and growth inhibition","volume":"53","author":"Shabahang","year":"1993","journal-title":"Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Miro\u0144czuk-Chodakowska, I., and Witkowska, A.M. (2020). Evaluation of Polish wild mushrooms as beta-glucan sources. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17197299"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11","DOI":"10.24326\/asphc.2020.1.2","article-title":"Determination of optimum culture conditions for mycelial growth of Macrolepiota procera Mushroom","volume":"19","author":"Kibar","year":"2020","journal-title":"Acta Sci. Pol. Hortorum Cultus"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Bucurica, I.A., Dulama, I.D., Radulescu, C., Banica, A.L., and Stanescu, S.G. (2024). Heavy metals and associated risks of wild edible mushrooms consumption: Transfer factor, carcinogenic risk, and health risk index. J. Fungi, 10.","DOI":"10.3390\/jof10120844"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"15528","DOI":"10.1007\/s11356-017-9136-9","article-title":"Analysis of some metallic elements and metalloids composition and relationships in parasol mushroom Macrolepiota procera","volume":"24","author":"Falandysz","year":"2017","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chun, S., Gopal, J., Muthu, M., Pablos, P.A., and Omaye, S. (2021). Antioxidant activity of mushroom extracts\/polysaccharides\u2014Their antiviral properties and plausible antiCOVID-19 properties. Antioxidants, 10.","DOI":"10.3390\/antiox10121899"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.foodchem.2008.09.033","article-title":"ORAC and TEAC assays comparison to measure the antioxidant capacity of food products","volume":"114","author":"Zulueta","year":"2009","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mizuno, M., and Minato, K.I. (2024). Anti-inflammatory and immunomodulatory properties of polysaccharides in mushrooms. Curr. Opin. Biotechnol., 86.","DOI":"10.1016\/j.copbio.2024.103076"},{"key":"ref_31","first-page":"316","article-title":"Lipoxygenase and cyclooxygenase pathways and colorectal cancer prevention","volume":"8","author":"Rao","year":"2012","journal-title":"Curr. Color. Cancer Rep."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chun, K.S., Kim, E.H., Kim, D.H., Song, N.Y., Kim, W., Na, H.K., and Surh, Y.J. (2024). Targeting cyclooxygenase-2 for chemoprevention of inflammation-associated intestinal carcinogenesis: An update. Biochem. Pharmacol., 228.","DOI":"10.1016\/j.bcp.2024.116259"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Li, C., Wu, G., Zhao, H., Dong, N., Wu, B., Chen, Y., and Lu, Q. (2021). Natural-derived polysaccharides from plants, mushrooms, and seaweeds for the treatment of inflammatory bowel disease. Front. Pharmacol., 12.","DOI":"10.3389\/fphar.2021.651813"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Nowacka-Jechalke, N., Kanak, S., Moczulski, M., Martyna, A., Kubi\u0144ski, K., Mas\u0142yk, M., Szpakowska, N., Kaczy\u0144ski, Z., Nowak, R., and Olech, M. (2023). Crude polysaccharides from wild-growing Armillaria mellea\u2014Chemical composition and antidiabetic, anti-inflammatory, antioxidant, and antiproliferative potential. Appl. Sci., 13.","DOI":"10.3390\/app13063853"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.carbpol.2019.01.064","article-title":"Polysaccharides as potential anticancer agents\u2014A review of their progress","volume":"210","author":"Khan","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1080\/14786419.2018.1466129","article-title":"Bioactive mushroom polysaccharides as antitumor: An overview","volume":"33","author":"Pandya","year":"2019","journal-title":"Nat. Prod. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1039\/c2fo10279j","article-title":"Antitumor activity of mushroom polysaccharides: A review","volume":"3","author":"Ren","year":"2012","journal-title":"Food Funct."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Bentharavithana, J., Islam, T., and Xu, B. (2025). medicinal mushrooms in colon cancer therapy: Mechanisms of action of bioactive compounds and therapeutic potential. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26115304"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1615\/IntJMedMushr.v15.i3.70","article-title":"Enhanced antiproliferative effects of aqueous extracts of some medicinal mushrooms on colon cancer cells","volume":"15","author":"Arora","year":"2013","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1615\/IntJMedMushrooms.2018028589","article-title":"Potential anticancer activity of the parasol mushroom, Macrolepiota procera (Agaricomycetes), against the A549 human lung cancer cell line","volume":"20","author":"Kaygusuz","year":"2018","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.jfda.2016.01.008","article-title":"Evaluation of metal concentration and antioxidant, antimicrobial, and anticancer potentials of two edible mushrooms Lactarius deliciosus and Macrolepiota procera","volume":"24","year":"2016","journal-title":"J. Food Drug Anal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7678","DOI":"10.1039\/C9FO01900F","article-title":"Comparative investigation on edible mushrooms Macrolepiota mastoidea, M. rhacodes and M. procera: Functional foods with diverse biological activities","volume":"10","author":"Fernandes","year":"2019","journal-title":"Food Funct."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric method for determination of sugars and related substances","volume":"28","author":"Dubois","year":"1956","journal-title":"Anal. Chem."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"He, C., Zhang, R., Jia, X., Dong, L., Ma, Q., Zhao, D., Sun, Z., Zhang, M., and Huang, F. (2022). Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.993828"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_46","first-page":"501","article-title":"Influence of different extraction procedures on the antiradical activity and phenolic profile of Rosa rugosa petals","volume":"69","author":"Olech","year":"2012","journal-title":"Acta Pol. Pharm."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1002\/elps.200600783","article-title":"Determination of neutral carbohydrates by CZE with direct uv detection","volume":"28","author":"Rovio","year":"2007","journal-title":"Electrophoresis"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"\u0141ubek-Nguyen, A., Olech, M., Nowacka-Jechalke, N., Martyna, A., Kubi\u0144ski, K., Mas\u0142yk, M., Moczulski, M., and Kanak, S. (2022). Crude polysaccharide fraction from Rosa rugosa Thunb. root\u2014Chemical characterisation, enzyme inhibitory, antioxidant and antiproliferative activity. Appl. Sci., 12.","DOI":"10.3390\/app121910126"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Dienait\u0117, L., Pukalskas, A., Pukalskien\u0117, M., Pereira, C.V., Matias, A.A., and Venskutonis, P.R. (2020). Phytochemical composition, antioxidant and antiproliferative activities of defatted sea buckthorn (Hippopha\u00eb rhamnoides L.) berry pomace fractions consecutively recovered by pressurized ethanol and water. Antioxidants, 9.","DOI":"10.3390\/antiox9040274"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Olech, M., \u0141yko, L., and Nowak, R. (2020). Influence of accelerated solvent extraction conditions on the LC-ESI-MS\/MS polyphenolic profile, triterpenoid content, and antioxidant and anti-lipoxygenase activity of Rhododendron luteum sweet leaves. Antioxidants, 9.","DOI":"10.3390\/antiox9090822"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.jep.2005.08.063","article-title":"Antioxidant and 15-lipoxygenase inhibitory activities of the malian medicinal plants Diospyros abyssinica (Hiern) F. White (Ebenaceae), Lannea velutina A. Rich (Anacardiaceae) and Crossopteryx febrifuga (Afzel) Benth. (Rubiaceae)","volume":"104","author":"Maiga","year":"2006","journal-title":"J. Ethnopharmacol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/20\/9978\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T15:23:02Z","timestamp":1760973782000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/20\/9978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,14]]},"references-count":51,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["ijms26209978"],"URL":"https:\/\/doi.org\/10.3390\/ijms26209978","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,14]]}}}