{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T23:25:39Z","timestamp":1761175539912,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca","award":["881\/31"],"award-info":[{"award-number":["881\/31"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The involvement of the serotoninergic system in the pathogenesis of calcific aortic stenosis introduced a novel dimension to our understanding of this complex cardiovascular condition. This study aimed to assess serotonin (5-HT) and its main metabolite, 5-Hydroxyindoleacetic acid (5-HIAA) in patients with severe aortic valve stenosis (AS). The study employed a case\u2013control design, including 76 patients who underwent transthoracic echocardiography, computed tomography (CT), and peripheral blood sampling. Serum concentrations of 5-HT and 5-HIAA were quantified using enzyme-linked immunosorbent assay (ELISA). The severe aortic valve stenosis group exhibited significantly elevated levels of 5-HT and 5-HIAA compared to the control group (5-HT 1066.5 ng\/mL (IQR = 961.9\u20131112 ng\/mL) vs. 977.4 ng\/mL (IQR = 394.3\u20131097.9 ng\/mL); p = 0.034 and 5-HIAA 57 \u00b1 12.7 ng\/mL vs. 47.5 \u00b1 15.3 ng\/mL; p = 0.004, respectively). Receiver operating characteristic (ROC) analysis revealed that 5-HT predicted severe AS with a sensitivity of 73.7% and specificity of 50% at a cut-off level &gt; 973.5 ng\/mL, whereas 5-HIAA exhibited a sensitivity of 86.8% and specificity of 47.4% when a cut-off level &gt; 45.49 ng\/mL was used. This study showed a significant elevation in the 5-HT and 5-HIAA among patients with severe AS, further supporting the potential involvement of the peripheral serotonergic system in the pathophysiology of this condition.<\/jats:p>","DOI":"10.3390\/ijms262110250","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T08:41:08Z","timestamp":1761122468000},"page":"10250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Peripheral Serotonergic Activation in Severe Aortic Stenosis: A Biochemical Perspective"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1169-3020","authenticated-orcid":false,"given":"Denisa Bianca","family":"Mercean","sequence":"first","affiliation":[{"name":"1st Department of Morpho-functional Sciences, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"},{"name":"Cardiology Department, Heart Institute \u201cN. St\u0103ncioiu\u201d, 400001 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1944-4088","authenticated-orcid":false,"given":"Raluca","family":"Tomoaia","sequence":"additional","affiliation":[{"name":"4th Department of Internal Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"},{"name":"Cardiology Department, Rehabilitation Hospital, 400347 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-1325","authenticated-orcid":false,"given":"Ioana","family":"Berindan-Neagoe","sequence":"additional","affiliation":[{"name":"Genomics Department, MEDFUTURE Institute of Biomedical Research, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 23 Marinescu Street, 400337 Cluj-Napoca, Romania"},{"name":"Doctoral School, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 8 Victor Babe\u015f Street, 400012 Cluj-Napoca, Romania"},{"name":"Academy of Medical Sciences, 1 Ion C. Br\u0103tianu Boulevard, 3rd Sector, 030167 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5725-662X","authenticated-orcid":false,"given":"Liviu\u0163a","family":"Budi\u015fan","sequence":"additional","affiliation":[{"name":"Genomics Department, MEDFUTURE Institute of Biomedical Research, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, 23 Marinescu Street, 400337 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1744-7952","authenticated-orcid":false,"given":"Dana","family":"Pop","sequence":"additional","affiliation":[{"name":"4th Department of Internal Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"},{"name":"Cardiology Department, Rehabilitation Hospital, 400347 Cluj-Napoca, Romania"},{"name":"Doctoral School, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 8 Victor Babe\u015f Street, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adela Mihaela","family":"\u0218erban","sequence":"additional","affiliation":[{"name":"Cardiology Department, Heart Institute \u201cN. St\u0103ncioiu\u201d, 400001 Cluj-Napoca, Romania"},{"name":"4th Department of Internal Medicine, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen Stanca","family":"Melincovici","sequence":"additional","affiliation":[{"name":"1st Department of Morpho-functional Sciences, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"},{"name":"Radiology and Imaging Department, County Emergency Hospital, 400006 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen Mihaela","family":"Mihu","sequence":"additional","affiliation":[{"name":"1st Department of Morpho-functional Sciences, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania"},{"name":"Radiology and Imaging Department, County Emergency Hospital, 400006 Cluj-Napoca, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1093\/eurheartj\/ehab395","article-title":"2021 ESC\/EACTS Guidelines for the management of valvular heart disease","volume":"43","author":"Vahanian","year":"2021","journal-title":"Eur. 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Heart Assoc."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10250\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T08:41:40Z","timestamp":1761122500000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,22]]},"references-count":23,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["ijms262110250"],"URL":"https:\/\/doi.org\/10.3390\/ijms262110250","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,22]]}}}