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Horse chestnut seed and ginseng root extracts stimulated lipase activity the most, while ivy leaf and fenugreek seed extracts had an inhibitory effect. The strongest antiradical activity (ABTS+\u2022\u20132,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) test) was exhibited by horse chestnut seed extract and the weakest by the soapwort root extract. Antioxidant activity strongly positively correlated with the total amount of phenols. Experimental studies showed that the surface properties of saponins present in extracts influence emulsion stabilization and have a moderate stimulating or inhibitory effect on pancreatic lipase activity. Computational studies demonstrated that lipase activity is stimulated by stabilizing an oil emulsion in the case of all studied saponins. Depending on the type of saponin, activation or inhibition of lipase was observed due to the immediate ligand\u2013enzyme interactions.<\/jats:p>","DOI":"10.3390\/ijms262110254","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T01:14:02Z","timestamp":1761182042000},"page":"10254","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antiradical and Antioxidant Activity and Stimulation of Pancreatic Lipase by Extracts Obtained from Saponin-Rich Raw Materials: Experimental and In Silico Study"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-017X","authenticated-orcid":false,"given":"Zbigniew","family":"Sroka","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8466-4098","authenticated-orcid":false,"given":"Karina","family":"Kapusta","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, Tougaloo College, Tougaloo, MS 39174, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9760-5240","authenticated-orcid":false,"given":"Klaudia","family":"Sucha\u0144ska","sequence":"additional","affiliation":[{"name":"Student Faculty of Pharmacy, Wroc\u0142aw Medical University, Borowska 211, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7754-8969","authenticated-orcid":false,"given":"Wojciech","family":"Ko\u0142odziejczyk","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Physics and Atmospheric Sciences, Jackson State University, Jackson, MS 39217, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"\u017bbikowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2206-6591","authenticated-orcid":false,"given":"Andrzej","family":"Gamian","sequence":"additional","affiliation":[{"name":"Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, 53-114 Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7248-7586","authenticated-orcid":false,"given":"Kacper","family":"Strzelczyk","sequence":"additional","affiliation":[{"name":"Student Faculty of Pharmacy, Wroc\u0142aw Medical University, Borowska 211, Wroc\u0142aw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Gle\u0144sk","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7693-9469","authenticated-orcid":false,"given":"Kamil","family":"Wojciechowski","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Warmia and Mazury in Olsztyn, Pl. \u0141\u00f3dzki 4, 10-721 Olsztyn, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.3748\/wjg.15.1673","article-title":"Chronic pancreatitis: Maldigestion, intestinal ecology and intestinal inflammation","volume":"15","author":"Pezzilli","year":"2009","journal-title":"World J. Gastroenterol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1097\/MPA.0000000000001733","article-title":"Effect of pancrelipase therapy on exocrine pancreatic insufficiency symptoms and coefficient of fat absorption associated with chronic pancreatitis","volume":"50","author":"Barkin","year":"2021","journal-title":"Pancreas"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13066","DOI":"10.1111\/ijcp.13066","article-title":"Introduction and practical approach to exocrine pancreatic insufficiency for the practicing clinician","volume":"72","author":"Othman","year":"2018","journal-title":"Int. J. Clin. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"9043","DOI":"10.1039\/D1FO00786F","article-title":"Gastric lipase can significantly increase lipolysis and carotenoid bioaccessibility from plant food matrices in the harmonized INFOGEST static in vitro digestion model","volume":"12","author":"Iddir","year":"2021","journal-title":"Food Funct."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/0016-5085(93)90908-U","article-title":"Secretion and contribution to lipolysis of gastric and pancreatic lipases during a test meal in humans","volume":"105","author":"Carriere","year":"1993","journal-title":"Gastroenterology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1016\/0016-5085(94)90559-2","article-title":"Pancreatic triglyceride lipase and colipase: Insight into dietary fat digestion","volume":"107","author":"Lowe","year":"1994","journal-title":"Gastroenterology"},{"key":"ref_7","first-page":"75","article-title":"Preparation of pancreatin and purification of lipase from hog pancreas","volume":"9","author":"Chang","year":"1985","journal-title":"Proc. Natl. Sci. Counc. Repub. China B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"15616","DOI":"10.1021\/jp1076406","article-title":"Role of the bile salt surfactant sodium cholate in enhancing the aqueous dispersion stability of single-walled carbon nanotubes: A molecular dynamics simulation study","volume":"114","author":"Lin","year":"2010","journal-title":"J. Phys. Chem. B"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0376-7388(94)00163-S","article-title":"The activity of lipase from Rhizopus sp. in native form and after immobilization on hollow-fiber membranes","volume":"97","author":"Sroka","year":"1994","journal-title":"J. Memb. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ejpb.2022.05.003","article-title":"Digestion of lipid excipients and lipid-based nanocarriers by pancreatic lipase and pancreatin","volume":"176","author":"To","year":"2022","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Toth, G.D., Kazsoki, A., Gyarmati, A., Szilagyi, A., Vasvari, G., Katona, G., Szente, L., Zelk\u00f3, R., Poppe, L., and Balogh-Weiser, D. (2021). Nanofibrous formulation of cyclodextrin stabilized lipases for efficient pancreatin replacement therapies. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13070972"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1002\/fft2.296","article-title":"Conventional and advanced extraction methods of some bioactive compounds with health benefits of food and plant waste: A comprehensive review","volume":"4","author":"Waseem","year":"2023","journal-title":"Food Front."},{"key":"ref_13","unstructured":"Phillipson, J.D. (1995). Saponins. Chemistry and Pharmacology of Natural Products, Cambridge University Press."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"100751","DOI":"10.1016\/j.fochx.2023.100751","article-title":"Fabrication and characterization of novel thermoresponsive emulsion gels and oleogels stabilizied by assembling nanofibrous from dual natural triterpenoid saponins","volume":"18","author":"Liu","year":"2023","journal-title":"Food Chem. X"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1002\/fsn3.3983","article-title":"Dietary phenolic compound as promising therapeutic agents for diabetes and its complications: A comprehensive review","volume":"12","author":"Aryal","year":"2024","journal-title":"Food Sci. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"del Carmen Villegas-Aguilar, M., Fern\u00e1ndez-Ochoa, \u00c1., de la Luz C\u00e1diz-Gurrea, M., Pimentel-Moral, S., Lozano-S\u00e1nchez, J., Arr\u00e1ez-Rom\u00e1n, D., and Segura-Carretero, A. (2020). Pleiotropic biological effects of dietary phenolic compounds and their metabolites on energy metabolism, inflammation and aging. Molecules, 25.","DOI":"10.3390\/molecules25030596"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"105003","DOI":"10.1016\/j.arabjc.2023.105003","article-title":"Micro-titer plate assay for measurement of total phenolic and total flavonoid contents in medicinal plant extracts","volume":"16","author":"Sari","year":"2023","journal-title":"Arab. J. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kiokias, S., and Oreopoulou, V. (2022). Review on the antioxidant activity of phenolics in o\/w emulsions along with the impact of a few important factors on their interfacial behavior. Colloids Interfaces, 6.","DOI":"10.3390\/colloids6040079"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"102145","DOI":"10.1016\/j.cis.2020.102145","article-title":"Surface activity and foaming properties of saponin-rich plants extracts","volume":"279","author":"Wojciechowski","year":"2020","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.colsurfa.2010.11.027","article-title":"Dynamic interfacial tensions of dietary oils","volume":"382","author":"Dopierala","year":"2011","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_21","first-page":"1529","article-title":"Rapid determination of \u03b1-hederin and hederacoside C in extracts of Hedera helix leaves available in the Czech Republic and Poland","volume":"10","author":"Opletal","year":"2015","journal-title":"Nat. Prod. Commun."},{"key":"ref_22","first-page":"211","article-title":"Horse-chestnut seed","volume":"Volume 2","author":"Bradley","year":"2006","journal-title":"British Herbal Compendium"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"18007","DOI":"10.1074\/jbc.271.30.18007","article-title":"Lipase Activation by Nonionic Detergents","volume":"271","author":"Hermoso","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"He, Y., Hu, Z., Li, A., Zhu, Z., Yang, N., Ying, Z., He, J., Wang, C., Yin, S., and Cheng, S. (2019). Recent Advances in Biotransformation of Saponins. Molecules, 24.","DOI":"10.3390\/molecules24132365"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0022-2275(20)34490-4","article-title":"On the interactions between pancreatic lipase and colipase and the substrate, and the importance of bile salts","volume":"16","author":"Borgstrom","year":"1975","journal-title":"J. Lipid Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.colsurfa.2011.03.058","article-title":"Superior thermal stability gel emulsion produced by low concentration Gemini surfactant","volume":"384","author":"Fan","year":"2011","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/0014-5793(93)81340-6","article-title":"Bile salt-stimulated lipase in human milk. Evidence that bile salt induces lipid lipid binding and activation via binding and activation via binding to different sites","volume":"323","author":"Hernell","year":"1993","journal-title":"FEBS Lett."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Akbari, S., Abdurahman, N.H., and Kudrashou, V. (2023). Surface activity and emulsification properties of saponins as biosurfactants. Advancement in Biosurfactants Research, Springer International Publishing.","DOI":"10.1007\/978-3-031-21682-4_7"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1208\/s12249-020-01683-3","article-title":"The effect of enzymes and sodium lauryl sulfate on the surface tension of dissolution media: Toward understanding the solubility and dissolution of carvedilol","volume":"21","author":"Hamed","year":"2020","journal-title":"AAPS PharmSciTech"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1007\/s11743-017-2016-y","article-title":"Surface Tension and adsorption studies by drop profile analysis tensiometry","volume":"20","author":"Kairaliyeva","year":"2017","journal-title":"J. Surfactants Deterg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1021\/acs.langmuir.2c03511","article-title":"Surfactant adsorption layers: Experiments and Modeling","volume":"39","author":"Kovalchuk","year":"2023","journal-title":"Langmuir"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"9525","DOI":"10.1021\/j100176a089","article-title":"Interaction of bile salts with hexadecyltrimethylammonium bromide and sodium dodecyl sulfate","volume":"95","author":"Jana","year":"1991","journal-title":"J. Phys. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1515\/znc-1997-3-415","article-title":"Stimulation of pancreatic lipase activity by saponins isolated from Medicago sativa L","volume":"52","author":"Sroka","year":"1997","journal-title":"Z. Naturforsch. C J. Biosci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.molcatb.2006.02.012","article-title":"Inhibition of Candida rugosa lipase by saponins, flavonoids and alkaloids","volume":"40","author":"Ruiz","year":"2006","journal-title":"J. Mol. Catal. B Enzym."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.jcis.2019.12.108","article-title":"Role of interfacial elasticity for the rheological properties of saponin-stabilized emulsions","volume":"564","author":"Tsibranska","year":"2020","journal-title":"J. Colloid Interface Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100400","DOI":"10.1016\/j.colcom.2021.100400","article-title":"Surface activity of the oat, horse chestnut, cowherb, soybean, quinoa, and soapwort extracts\u2014Is it only due to saponins?","volume":"42","author":"Stochmal","year":"2021","journal-title":"Colloid Interface Sci. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.5650\/jos.ess21081","article-title":"Effect of bile salts on the interfacial dilational rheology of lecithin in the lipid digestion process","volume":"70","author":"Mekkaoui","year":"2021","journal-title":"J. Oleo Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/0009-8981(66)90215-4","article-title":"A specific method for serum lipase glycerol ester hydrolase (EC 3.1.1.3) determination","volume":"13","author":"Tietz","year":"1966","journal-title":"Clin. Chim. Acta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.foodchem.2016.12.008","article-title":"Combined microplate-ABTS and HPLC-ABTS analysis of tomato and pepper extracts reveals synergetic and antagonist effects of their lipophilic antioxidative components","volume":"223","author":"Guffond","year":"2017","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3470","DOI":"10.1021\/jf703723s","article-title":"High-throughput methods to assess lipophilic and hydrophilic antioxidant capacity of food extracts in vitro","volume":"56","author":"Giuffrida","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/S0927-7757(96)03857-5","article-title":"Determination of equilibrium surface tension values by extrapolation via long time approximation","volume":"122","author":"Makievski","year":"1997","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S1383-7303(01)80038-7","article-title":"Drop and Bubble Shape Analysis as a Tool for Dilational Rheological Studies of Interfacial layers","volume":"11","author":"Loglio","year":"2001","journal-title":"Stud. Interf. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Bowers, K.J., Chow, D.E., Xu, H., Dror, R.O., Eastwood, M.P., Gregersen, B.A., Klepeis, J.L., Kolossvary, I., Moraes, M.A., and Sacerdoti, F.D. (2006, January 11\u201317). Scalable Algorithms for Molecular Dynamics Simulations on Commodity Clusters. Proceedings of the SC\u201906: Proceedings of the 2006 ACM\/IEEE Conference on Supercomputing, Tampa, FL, USA.","DOI":"10.1109\/SC.2006.54"},{"key":"ref_44","unstructured":"(2024, May 01). RCSB Protein Data Bank. Available online: https:\/\/www.rcsb.org\/."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1007\/s10822-007-9133-z","article-title":"Epik: A software program for pK(a) prediction and protonation state generation for drug-like molecules","volume":"21","author":"Shelley","year":"2007","journal-title":"J. Comput. Aided Mol. Des."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4291","DOI":"10.1021\/acs.jctc.1c00302","article-title":"OPLS4: Improving Force Field Accuracy on Challenging Regimes of Chemical Space","volume":"17","author":"Lu","year":"2021","journal-title":"J. Chem. Theory Comput."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6177","DOI":"10.1021\/jm051256o","article-title":"Extra precision glide: Docking and scoring incorporating a model of hydrophobic enclosure for protein-ligand complexes","volume":"49","author":"Friesner","year":"2006","journal-title":"J. Med. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10254\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T04:27:25Z","timestamp":1761280045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,22]]},"references-count":47,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["ijms262110254"],"URL":"https:\/\/doi.org\/10.3390\/ijms262110254","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,22]]}}}