{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T04:18:10Z","timestamp":1761365890164,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100021039","name":"Ross University School of Veterinary Medicine","doi-asserted-by":"crossref","award":["41003-21"],"award-info":[{"award-number":["41003-21"]}],"id":[{"id":"10.13039\/100021039","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Research Foundation (NRF), South Africa"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Falciparum malaria is a life-threatening vector-borne disease prevalent in tropical and subtropical regions. The complexity of severe malaria demands a thorough investigation of host\u2013parasite interactions. Twenty male Sprague Dawley rats were divided into two groups: uninfected controls and Plasmodium berghei-infected rats, infected via intraperitoneal injection of parasitized red blood cells. Serum samples were analysed using high-resolution untargeted Gas Chromatography\u2013Time-of-Flight Mass Spectrometry. Metabolomic analyses revealed altered metabolites and enriched metabolic pathways. Distinct metabolite profiles were observed between infected and control groups. Infected rats showed elevated urea levels and reduced concentrations of 1,5-anhydroglucitol, D-(+)-Talose, and arachidonic acid. Pathway analysis revealed significant enrichment of the glucose-alanine cycle, alpha-linolenic acid metabolism, and linoleic acid metabolism in infected rats. Minimal enrichment was observed in arachidonic acid metabolism and lactose biosynthesis. The upregulation of the glucose-alanine cycle suggests increased gluconeogenesis in response to parasite-induced glucose depletion and energy demand. Elevated urea indicates enhanced amino acid catabolism. These findings highlight the potential of metabolomics as a diagnostic tool for malaria detection and prognosis.<\/jats:p>","DOI":"10.3390\/ijms262110324","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:47:36Z","timestamp":1761266856000},"page":"10324","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of a Non-Targeted Metabolomics Study in Plasmodium berghei-Infected Rats: Towards Unravelling Metabolic Alterations During Malaria Infection"],"prefix":"10.3390","volume":"26","author":[{"given":"Zoxolo Nokulunga","family":"Mbuli","sequence":"first","affiliation":[{"name":"School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 3630, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1384-2026","authenticated-orcid":false,"given":"Innocent Siyanda","family":"Ndlovu","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 3630, South Africa"},{"name":"Non-Communicable Diseases Research Unit, South African Medical Research Council, Parow Valley 7505, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bubuya","family":"Masola","sequence":"additional","affiliation":[{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, School of Veterinary Medicine, Ross University, Basseterre KN0101, Saint Kitts and Nevis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samson","family":"Mukaratirwa","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 3630, South Africa"},{"name":"One Health Center for Zoonoses and Tropical Veterinary Medicine, School of Veterinary Medicine, Ross University, Basseterre KN0101, Saint Kitts and Nevis"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"ref_1","unstructured":"WHO (2024). 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