{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:49:33Z","timestamp":1787028573809,"version":"build-2736575974"},"reference-count":68,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T00:00:00Z","timestamp":1761782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["25-14-00047"],"award-info":[{"award-number":["25-14-00047"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Circular RNAs (circRNAs) are non-coding RNAs that can significantly influence the regulation of gene expression in health and disease, including ischemic stroke. We identified 597 differentially expressed circRNAs (DECs) (fold change &gt; 1.5; Padj &lt; 0.05) in the striatum region encompassing the ischemic lesion and penumbra 24 h after ischemia\u2013reperfusion injury (tMCAO) in rats, according to high-throughput RNA sequencing data (RNA-Seq). The DECs predominantly increased expression levels relative to those in sham-operated animals. In this study, we also compared these data with DECs we previously identified in the frontal cortex region containing the penumbra and healthy tissue. Furthermore, we bioinformatically constructed a network of competitive circRNA-microRNA-mRNA interactions characterizing the possible functions of DECs in brain areas with varying degrees of ischemic injury. We found that in both tissues, the identified DECs were involved in regulating the expression of genes associated with inflammation and neurotransmission. Moreover, in the striatum, most DECs decreased their expression, while in the frontal cortex, most DECs increased their expression. Thus, we demonstrated different circRNA activities in brain areas with varying degrees of injury. This result may indicate a role for these molecules in regulating brain cell responses, including those important for functional recovery after cerebral ischemia.<\/jats:p>","DOI":"10.3390\/ijms262110555","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T01:23:59Z","timestamp":1761873839000},"page":"10555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential Expression of Circular RNAs in Rat Brain Regions with Various Degrees of Damage After Ischemia\u2013Reperfusion"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3284-1249","authenticated-orcid":false,"given":"Ivan V.","family":"Mozgovoy","sequence":"first","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina V.","family":"Tsareva","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina E.","family":"Denisova","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasily V.","family":"Stavchansky","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid V.","family":"Gubsky","sequence":"additional","affiliation":[{"name":"Department of Neurology, Neurosurgery and Medical Genetics, Pirogov Russian National Research Medical University, Ostrovitianov Str. 1, Moscow 117997, Russia"},{"name":"Federal Center for the Brain and Neurotechnologies, Federal Biomedical Agency, Ostrovitianov Str. 1, Building 10, Moscow 117997, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2789-2419","authenticated-orcid":false,"given":"Lyudmila V.","family":"Dergunova","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana A.","family":"Limborska","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6964-3405","authenticated-orcid":false,"given":"Ivan B.","family":"Filippenkov","sequence":"additional","affiliation":[{"name":"Laboratory of Human Molecular Genetics, National Research Center \u201cKurchatov Institute\u201d, Kurchatov Sq. 2, Moscow 123182, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1186\/s12943-020-01286-3","article-title":"Circular RNA: Metabolism, Functions and Interactions with Proteins","volume":"19","author":"Zhou","year":"2020","journal-title":"Mol. 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