{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T14:10:12Z","timestamp":1762179012494,"version":"build-2065373602"},"reference-count":57,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T00:00:00Z","timestamp":1761868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Natural Science Foundation of China, Youth Fund Project","award":["32302996"],"award-info":[{"award-number":["32302996"]}]},{"name":"Beijing Natural Science Foundation for Young Science","award":["6234045"],"award-info":[{"award-number":["6234045"]}]},{"name":"National Engineering Research Center for Freshwaters","award":["PT2024-19"],"award-info":[{"award-number":["PT2024-19"]}]},{"name":"Beijing Key Laboratory of Fisheries Biotechnology","award":["PT2024-20"],"award-info":[{"award-number":["PT2024-20"]}]},{"name":"National Freshwater Aquatic Germplasm Resource Bank","award":["FGRC: 18537"],"award-info":[{"award-number":["FGRC: 18537"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>After intensive artificial selection, the development of celestial eyes in goldfish involves the eyeballs protuberating and turning upwards. Thus, the celestial eye goldfish is an excellent model for both evolutionary and human ocular disease studies. Here, two mapping populations of goldfish with segregating eye phenotypes in the offspring were constructed. Through whole-genome sequencing and RNA-seq for eyeball samples, a premature stop codon in Exon 38 of the LRP2 gene was identified as the top candidate mutation for the celestial eye in goldfish. Fatty acid metabolism and epidermal cells, especially keratocyte-related functions, were inhibited in the eyeballs of celestial eye goldfish, while inflammatory reactions and extracellular matrix secretions were stimulated. These results suggest the dysfunction of the cornea in the celestial eye goldfish, and the same for the retina, which could be the results of the truncated LRP2 protein. In addition, the same gene, LRP2, is in charge of similar phenotypes (celestial eye and telescope eye) in goldfish, but these phenotypes have no shared mutations. In conclusion, the candidate mutation for the celestial eye in goldfish was identified by this study for the first time, and parallel evolutions of similar phenotypes at the molecular level under artificial selection were observed. These findings provide insights into the developmental and evolutionary processes of morphological changes in the eyes of goldfish.<\/jats:p>","DOI":"10.3390\/ijms262110625","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:15:14Z","timestamp":1762175714000},"page":"10625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Independent Mutations in the LRP2 Gene Mediating Telescope Eyes and Celestial Eyes in Goldfish"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5631-6501","authenticated-orcid":false,"given":"Rongni","family":"Li","sequence":"first","affiliation":[{"name":"Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, No. 18 Jiaomen Rd., Beijing 100097, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7331-1550","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Animal Breeding, China Agricultural University, No. 2 Yuanmingyuan West Rd., Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, No. 18 Jiaomen Rd., Beijing 100097, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6775-5190","authenticated-orcid":false,"given":"Jingyi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Street, Wuhan 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, S.Y., Luo, J., Murphy, R.W., Wu, S.F., Zhu, C.L., Gao, Y., and Zhang, Y.P. 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