{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T14:39:01Z","timestamp":1782225541183,"version":"3.54.5"},"reference-count":57,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Core Research for Evolutional Science and Technology (CREST) program","award":["JPMJCR22E3"],"award-info":[{"award-number":["JPMJCR22E3"]}]},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["50425708"],"award-info":[{"award-number":["50425708"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["00768880"],"award-info":[{"award-number":["00768880"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","award":["20335483"],"award-info":[{"award-number":["20335483"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003478","name":"Japanese Ministry of Health, Labour and Welfare","doi-asserted-by":"crossref","award":["202410072A"],"award-info":[{"award-number":["202410072A"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003478","name":"Japanese Ministry of Health, Labour and Welfare","doi-asserted-by":"crossref","award":["2023C-16"],"award-info":[{"award-number":["2023C-16"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Daiichi Sankyo Science Foundation"},{"DOI":"10.13039\/100020236","name":"Japan Foundation for Pediatric Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100020236","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Mishima Kaiun Memorial Foundation"},{"name":"Mitsubishi Tanabe Science Foundation"},{"name":"Otsuka Science Foundation"},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Characterized by social communication deficits and the presence of restricted and repetitive behaviors, autism spectrum disorder (ASD) is a significant neurodevelopmental condition. Genetic studies have revealed a strong association between ASD and numerous mutations that alter the function of key proteins, either through activation or inactivation. These alterations are widely hypothesized to affect neuronal morphogenesis; however, a comprehensive understanding of the specific molecular cascades driving these cellular and symptomatic changes remains lacking. In this study, we report for the first time that signaling through the atypical Rho family guanine-nucleotide exchange factor (GEF) Dock7 and ErbB2, an activator acting upstream of Dock7, drives the excessive elongation of neuronal processes observed in association with the ASD- and intellectual disability (ID)-linked semaphorin-5A (Sema5A) Arg676Cys variant (p.Arg676Cys). Knockdown of Dock7 using short hairpin RNA or inhibition of ErbB2 kinase signaling with a specific chemical inhibitor reduced this excessive process elongation in primary cortical neurons. Similar results were obtained in the N1E-115 cell line, a neuronal cell model that undergoes neuronal morphological differentiation. Moreover, inhibition of ErbB2-Dock7 signaling specifically decreased the overactivation of the downstream molecules Rac1 and Cdc42. These findings indicate that the ErbB2\u2013Dock7 signaling axis plays a role in mediating the aberrant neuronal morphology associated with the ASD- and ID-linked Sema5A p.Arg676Cys. Targeting this pathway may therefore offer a potential approach to addressing the molecular and cellular developmental challenges observed in ASD.<\/jats:p>","DOI":"10.3390\/ijms262110656","type":"journal-article","created":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:15:14Z","timestamp":1762175714000},"page":"10656","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The ErbB2\u2013Dock7 Signaling Axis Mediates Excessive Cell Morphogenesis Induced by Autism Spectrum Disorder- and Intellectual Disability-Associated Sema5A p.Arg676Cys"],"prefix":"10.3390","volume":"26","author":[{"given":"Mikito","family":"Takahashi","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2632-8488","authenticated-orcid":false,"given":"Hideji","family":"Yako","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaka","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuma","family":"Isa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9298-5799","authenticated-orcid":false,"given":"Yuki","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"},{"name":"Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3618-998X","authenticated-orcid":false,"given":"Junji","family":"Yamauchi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience and Neurology, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192-0392, Japan"},{"name":"Department of Biological Sciences, Tokyo College of Biotechnology, Ota, Tokyo 157-8535, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/j.chc.2010.07.005","article-title":"Psychopathology, families, and culture: Autism","volume":"19","author":"Bernier","year":"2010","journal-title":"Child Adolesc. Psychiatr. Clin. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Mazza, M., Pino, M.C., Mariano, M., Tempesta, D., Ferrana, M., Berardis, D.D., Masedu, F., and Valenti, M. (2014). Affective and cognitive empathy in adolescents with autism spectrum disorder. Front. Hum. Neurosci., 8.","DOI":"10.3389\/fnhum.2014.00791"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.copsyc.2018.07.004","article-title":"The state of research on the genetics of autism spectrum disorder: Methodological, clinical and conceptual progress","volume":"27","author":"Arnett","year":"2019","journal-title":"Curr. Opin. Psychol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/s40653-019-00253-5","article-title":"Trauma and autism spectrum disorder: Review, proposed treatment adaptations and future directions","volume":"12","author":"Peterson","year":"2019","journal-title":"J. Child. Adolesc. Trauma"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13229-017-0121-4","article-title":"Environmental risk factors for autism: An evidence-based review of systematic reviews and meta-analyses","volume":"8","author":"Modabbernia","year":"2017","journal-title":"Mol. Autism"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s00401-017-1736-4","article-title":"Autism spectrum disorder: Neuropathology and animal models","volume":"134","author":"Varghese","year":"2017","journal-title":"Acta Neuropathol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Wang, L., Wang, B., Wu, C., Wang, J., and Sun, M. (2023). Autism spectrum disorder: Neurodevelopmental risk factors, biological mechanism, and precision therapy. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24031819"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ayoub, G. (2025). Autism spectrum disorder as a multifactorial disorder: The interplay of genetic factors and inflammation. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202506.1224.v1"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1073\/pnas.65.4.905","article-title":"Surface movements during the growth of single explanted neurons","volume":"65","author":"Bray","year":"1970","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Rigby, M.J., Gomez, T.M., and Puglielli, L. (2020). Glial cell-axonal growth cone interactions in neurodevelopment and regeneration. Front. Neurosci., 14.","DOI":"10.3389\/fnins.2020.00203"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1146\/annurev.ne.17.030194.001411","article-title":"Neuronal polarity","volume":"17","author":"Craig","year":"1994","journal-title":"Annu. Rev. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/nrn918","article-title":"Breaking the neuronal sphere: Regulation of the actin cytoskeleton in neuritogenesis","volume":"3","author":"Dotti","year":"2002","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1038\/nrn2056","article-title":"Neuronal polarity: From extracellular signals to intracellular mechanisms","volume":"8","author":"Arimura","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nrn3379","article-title":"Neurotrophin regulation of neural circuit development and function","volume":"14","author":"Park","year":"2013","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nrd4337","article-title":"Semaphorins and plexins as therapeutic targets","volume":"13","author":"Worzfeld","year":"2014","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.tins.2008.11.006","article-title":"Axon guidance and synaptic maintenance: Preclinical markers for neurodegenerative disease and therapeutics","volume":"32","author":"Lin","year":"2009","journal-title":"Trends Neurosci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.conb.2020.12.014","article-title":"Semaphorins and plexins in central nervous system patterning: The key to it all?","volume":"66","author":"Limoni","year":"2021","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/bs.ctdb.2020.11.008","article-title":"New insights into the molecular mechanisms of axon guidance receptor regulation and signaling","volume":"142","author":"Zang","year":"2021","journal-title":"Curr. Top Dev. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/sj.embor.7400189","article-title":"Plexin-B3 is a functional receptor for semaphorin 5A","volume":"5","author":"Artigiani","year":"2004","journal-title":"EMBO Rep."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1523\/JNEUROSCI.4390-03.2004","article-title":"An oligodendrocyte lineage-specific semaphorin, Sema5A, inhibits axon growth by retinal ganglion cells","volume":"24","author":"Goldberg","year":"2004","journal-title":"J. Neurosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.neuron.2011.06.009","article-title":"Class 5 transmembrane semaphorins control selective mammalian retinal lamination and function","volume":"71","author":"Matsuoka","year":"2011","journal-title":"Neuron"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1038\/s41467-024-46725-7","article-title":"Structure and function of Semaphorin-5A glycosaminoglycan interactions","volume":"15","author":"Nagy","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1159\/000096040","article-title":"Constitutional downregulation of SEMA5A expression in autism","volume":"54","author":"Melin","year":"2006","journal-title":"Neuropsychobiology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1002\/ajmg.a.37445","article-title":"Multigenerational autosomal dominant inheritance of 5p chromosomal deletions","volume":"170","author":"Zhang","year":"2016","journal-title":"Am. J. Med. Genet. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1038\/ejhg.2015.211","article-title":"A de novo microdeletion of SEMA5A in a boy with autism spectrum disorder and intellectual disability","volume":"24","author":"Gueneau","year":"2016","journal-title":"Eur. J. Hum. Genet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00795-017-0161-z","article-title":"Autism spectrum disorder-associated genes and the development of dentate granule cells","volume":"50","author":"Ito","year":"2017","journal-title":"Med. Mol. Morphol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1212\/WNL.0b013e3181e396a8","article-title":"Familial cortical myoclonic tremor with epilepsy: The third locus (FCMTE3) maps to 5p","volume":"74","author":"Depienne","year":"2010","journal-title":"Neurology"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wang, Q., Liu, Z., Lin, Z., Zhang, R., Lu, Y., Su, W., Li, F., Xu, X., Tu, M., and Lou, Y. (2019). De novo germline mutations in SEMA5A associated with infantile spasms. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00605"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.bbr.2011.07.008","article-title":"Are Sema5a mutant mice a good model of autism? A behavioral analysis of sensory systems, emotionality and cognition","volume":"225","author":"Gunn","year":"2011","journal-title":"Behav. Brain Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e04390","DOI":"10.7554\/eLife.04390","article-title":"Semaphorin 5A inhibits synaptogenesis in early postnatal- and adult-born hippocampal dentate granule cells","volume":"3","author":"Duan","year":"2014","journal-title":"Elife"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"548","DOI":"10.3390\/pathophysiology30040040","article-title":"RhoG-binding domain of Elmo1 ameliorates excessive process elongation induced by autism spectrum disorder-associated Sema5A","volume":"30","author":"Okabe","year":"2023","journal-title":"Pathophysiology"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3092","DOI":"10.3390\/cimb46040194","article-title":"Autism spectrum disorder- and\/or intellectual disability-associated semaphorin-5A exploits the mechanism by which Dock5 signalosome molecules control cell shape","volume":"46","author":"Okabe","year":"2024","journal-title":"Curr. Issues Mol. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1146\/annurev.cellbio.20.022003.114619","article-title":"The engulfment process of programmed cell death in Caenorhabditis elegans","volume":"20","author":"Reddien","year":"2004","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cellsig.2009.09.036","article-title":"Cellular signaling of Dock family proteins in neural function","volume":"22","author":"Miyamoto","year":"2010","journal-title":"Cell. Signal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e973760","DOI":"10.4161\/21541248.2014.973760","article-title":"Epithelial junctions and Rho family GTPases: The zonular signalosome","volume":"5","author":"Citi","year":"2014","journal-title":"Small GTPases"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.sbi.2021.08.001","article-title":"Structural insights into the small GTPase specificity of the DOCK guanine nucleotide exchange factors","volume":"71","author":"Ihara","year":"2021","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.neuron.2006.07.020","article-title":"The Rac activator DOCK7 regulates neuronal polarity through local phosphorylation of stathmin\/Op18","volume":"51","author":"John","year":"2006","journal-title":"Neuron"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1083\/jcb.200709033","article-title":"ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration","volume":"181","author":"Yamauchi","year":"2008","journal-title":"J. Cell Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"28861","DOI":"10.1074\/jbc.M202927200","article-title":"Basic fibroblast growth factor evokes a rapid glutamate release through activation of the MAPK pathway in cultured cortical neurons","volume":"277","author":"Numakawa","year":"2002","journal-title":"J. Biol. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1007\/s11064-018-2674-9","article-title":"Psoralidin stimulates expression of immediate-early genes and synapse development in primary cortical neurons","volume":"43","author":"Hwang","year":"2018","journal-title":"Neurochem. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1073\/pnas.69.1.258","article-title":"Neurotransmitter synthesis by neuroblastoma clones","volume":"69","author":"Amano","year":"1972","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1083\/jcb.141.7.1625","article-title":"Molecular dissection of the Rho-associated protein kinase (p160ROCK)-regulated neurite remodeling in neuroblastoma N1E-115 cells","volume":"141","author":"Hirose","year":"1998","journal-title":"J. Cell Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"173815","DOI":"10.1016\/j.ejphar.2020.173815","article-title":"Role of RhoA-ROCK signaling in Parkinson\u2019s disease","volume":"894","author":"Iyer","year":"2021","journal-title":"Eur. J. Pharmacol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"113481","DOI":"10.1016\/j.bbr.2021.113481","article-title":"Role of RhoA\/ROCK signaling in Alzheimer\u2019s disease","volume":"414","author":"Cai","year":"2021","journal-title":"Behav. Brain Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mosaddeghzadeh, N., and Ahmadian, M.R. (2021). The RHO family GTPases: Mechanisms of regulation and signaling. Cells, 10.","DOI":"10.3390\/cells10071831"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"175207","DOI":"10.1016\/j.ejphar.2022.175207","article-title":"Antipsychotic-like effects of fasudil, a Rho-kinase inhibitor, in a pharmacologic animal model of schizophrenia","volume":"931","author":"Takase","year":"2022","journal-title":"Eur. J. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/s41572-019-0111-2","article-title":"Breast cancer","volume":"5","author":"Harbeck","year":"2019","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1038\/s41571-024-00924-9","article-title":"HER2-targeted therapies beyond breast cancer-an update","volume":"21","author":"Yoon","year":"2024","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"pe11","DOI":"10.1126\/stke.3252006pe11","article-title":"Neuregulin-1 and myelination","volume":"2006","author":"Lemke","year":"2006","journal-title":"Sci. STKE"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"178","DOI":"10.2174\/156720512799361637","article-title":"BACE1 dependent neuregulin processing: Review","volume":"9","author":"Fleck","year":"2012","journal-title":"Curr. Alzheimer Res."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kataria, H., Alizadeh, A., and Karimi-Abdolrezaee, S. (2019). Neuregulin-1\/ErbB network: An emerging modulator of nervous system injury and repair. Prog. Neurobiol., 180.","DOI":"10.1016\/j.pneurobio.2019.101643"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102092","DOI":"10.1016\/j.arr.2023.102092","article-title":"The mechanisms of perineuronal net abnormalities in contributing aging and neurological diseases","volume":"92","author":"Dong","year":"2023","journal-title":"Ageing Res. Rev."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1158\/0008-5472.CAN-24-1793","article-title":"The brain-penetrant pan-erbb inhibitor poziotinib effectively targets her2+ breast cancer brain metastases","volume":"85","author":"Lppolitov","year":"2025","journal-title":"Cancer Res."},{"key":"ref_54","first-page":"754","article-title":"Pan-Cancer Landscape and Analysis of ERBB2 Mutations Identifies Poziotinib as a Clinically Active Inhibitor and Enhancer of T-DM1 Activity","volume":"40","author":"Robichaux","year":"2022","journal-title":"Cancer Cell"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/978-1-61779-170-3_2","article-title":"Primary neurons in culture and neuronal cell lines for in vitro neurotoxicological studies","volume":"758","author":"Giordano","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2035","DOI":"10.1128\/MCB.00133-15","article-title":"Growth cone localization of the mRNA encoding the chromatin regulator HMGN5 modulates neurite outgrowth","volume":"35","author":"Moretti","year":"2015","journal-title":"Mol. Cell. Biol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Ishida, M., Ichikawa, R., Ohbuchi, K., Oizumi, H., Miyamoto, Y., and Yamauchi, J. (2025). A tardive dyskinesia drug target VMAT-2 participates in neuronal process elongation. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-97308-5"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10656\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T05:28:52Z","timestamp":1762234132000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/21\/10656"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,1]]},"references-count":57,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["ijms262110656"],"URL":"https:\/\/doi.org\/10.3390\/ijms262110656","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,1]]}}}