{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T05:24:29Z","timestamp":1762493069858,"version":"build-2065373602"},"reference-count":61,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund CAFS","doi-asserted-by":"crossref","award":["2025XT0702","2023TD39"],"award-info":[{"award-number":["2025XT0702","2023TD39"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the earmarked fund","award":["CARS-48-07"],"award-info":[{"award-number":["CARS-48-07"]}]},{"name":"Seed Industry Revitalization Project of Jiangsu Province","award":["JBGS [2021]118"],"award-info":[{"award-number":["JBGS [2021]118"]}]},{"name":"Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study conducted a bioinformatic analysis of two Hyaluronidase-like isoforms (Mn-HyaL1 and Mn-HyaL2) in Macrobrachium nipponense and investigated their phylogenetic relationships. The open reading frames of Mn-HyaL1 and Mn-HyaL2 were 1101 bp (encoding 366 amino acids) and 1164 bp (encoding 387 amino acids), respectively. Both isoforms exhibited similar conserved domains, with an amino acid sequence similarity of 60.21%. Quantitative PCR analysis revealed that the expression levels of Mn-HyaL1 and Mn-HyaL2 increased during the mid-to-late phase of each developmental stage, were higher during the reproductive season than in the non-reproductive season, and were more abundant in the hepatopancreas than in other tissues. RNA interference experiments targeting both genes simultaneously demonstrated that knockdown of Mn-HyaL2 significantly accelerated ovarian development in M. nipponense, indicating that Mn-HyaL genes function as negative regulators of ovarian maturation. A comparative analysis of multiple genes revealed the following descending order of potency in promoting ovarian development in M. nipponense: Mn-Cholesterol 7-desaturase &gt; Mn-Cathepsin L1. The order of potency in inhibiting ovarian development in M. nipponense, from strongest to weakest, was determined to be Mn-Gonad-inhibiting hormone &gt; Mn-HyaL2.<\/jats:p>","DOI":"10.3390\/ijms262110748","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T13:19:29Z","timestamp":1762348769000},"page":"10748","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Analysis of Hyaluronidase-like Genes in Ovarian Development of Macrobrachium nipponense and Comparative Evaluation with Other Key Regulatory Genes"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1089-7075","authenticated-orcid":false,"given":"Zhiming","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key 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Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China\u2019s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghao","family":"Ye","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Gong","sequence":"additional","affiliation":[{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sufei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China"},{"name":"Key Laboratory of Mariculture & Stock Enhancement in North China\u2019s Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China"},{"name":"Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, 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