{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T05:28:06Z","timestamp":1763184486465,"version":"3.45.0"},"reference-count":33,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T00:00:00Z","timestamp":1762992000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014271","name":"University of Life Sciences in Lublin","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100014271","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Post-translational modifications (phosphorylation and glutathionylation) not only assure protein diversity but are also responsible for the controlling of the biological activity of selected proteins in health and disease. The aim of the study was to monitor the profile of changes in molecular weight of proteins undergoing selected post-translational modifications by measurement of the intensity of phosphorylation and glutathionylation within the pregnancy course and parturition in cows with and without the retention of foetal membranes. The intensity of selected post-translational modifications was measured in bovine placental tissues collected during pregnancy (2nd, 4th, 5th, and 7th month, n = 4 per month) and parturition (not-retained foetal membranes (NRFM, n = 6) and retained foetal membranes (RFM, n = 6). Placental tissues were homogenised and used for the Phosphoprotein Phosphate Estimation Assay Kit and Western blotting analyses with adequate antibodies. The content of phosphorylated proteins was significantly higher (p &lt; 0.05) in the 2nd month as compared to other months, both in the maternal and foetal parts of the placenta. Moreover, no significant differences were observed between NRFM and RFM samples. The results of Western blotting showed the shift in molecular weight and indirect content of phosphorylated selected amino acids. Further research on the role of post-translational modifications in pregnancy and parturition may give new insight into their biochemical regulation.<\/jats:p>","DOI":"10.3390\/ijms262210984","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T09:59:09Z","timestamp":1763027949000},"page":"10984","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selected Post-Translational Modifications\u2014Phosphorylation and Glutathionylation\u2014As Factors Involved in the Regulation During the Pregnancy Course and Foetal Membrane Release in Cows"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2219-0701","authenticated-orcid":false,"given":"Jacek","family":"Wawrzykowski","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1984-6407","authenticated-orcid":false,"given":"Monika A.","family":"Jamio\u0142","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5196-4975","authenticated-orcid":false,"given":"Ewelina","family":"Kosztowny","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3053-0742","authenticated-orcid":false,"given":"Marta","family":"Kankofer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dutta, H., and Jain, N. (2023). Post-Translational Modifications and Their Implications in Cancer. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1240115"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e261","DOI":"10.1002\/mco2.261","article-title":"Protein Posttranslational Modifications in Health and Diseases: Functions, Regulatory Mechanisms, and Therapeutic Implications","volume":"4","author":"Zhong","year":"2023","journal-title":"MedComm"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"207691","DOI":"10.4061\/2011\/207691","article-title":"Small Changes Huge Impact: The Role of Protein Posttranslational Modifications in Cellular Homeostasis and Disease","volume":"2011","author":"Karve","year":"2011","journal-title":"J. Amino Acids"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1038\/s41570-020-00223-8","article-title":"Deciphering Protein Post-Translational Modifications Using Chemical Biology Tools","volume":"4","author":"Conibear","year":"2020","journal-title":"Nat. Rev. Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"baab012","DOI":"10.1093\/database\/baab012","article-title":"Post-Translational Modifications in Proteins: Resources, Tools and Prediction Methods","volume":"2021","author":"Ramazi","year":"2021","journal-title":"Database"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3892\/ijmm.2017.3036","article-title":"Lo The Crucial Role of Protein Phosphorylation in Cell Signaling and Its Use as Targeted Therapy (Review)","volume":"40","author":"Ardito","year":"2017","journal-title":"Int. J. Mol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6.17.1","DOI":"10.1002\/0471140856.tx0617s57","article-title":"Methods to Detect Protein Glutathionylation","volume":"57","author":"Poerschke","year":"2013","journal-title":"Curr. Protoc. Toxicol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3165","DOI":"10.1016\/j.bbagen.2013.02.009","article-title":"Protein Glutathionylation in Health and Disease","volume":"1830","author":"Ghezzi","year":"2013","journal-title":"Biochim. Biophys. Acta-Gen. Subj."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.ejps.2012.03.010","article-title":"S-Glutathionylation Signaling in Cell Biology: Progress and Prospects","volume":"46","author":"Pastore","year":"2012","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.3168\/jds.2017-13201","article-title":"The Effects of L-Type Amino Acid Transporter 1 on Milk Protein Synthesis in Mammary Glands of Dairy Cows","volume":"101","author":"Lin","year":"2018","journal-title":"J. Dairy Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.3168\/jds.2006-778","article-title":"Abundance and Phosphorylation State of Translation Initiation Factors in Mammary Glands of Lactating and Nonlactating Dairy Cows","volume":"90","author":"Toerien","year":"2007","journal-title":"J. Dairy Sci."},{"key":"ref_12","first-page":"3","article-title":"Growth Factors and Steroidogenesis in the Bovine Placenta","volume":"5","author":"Sousa","year":"2008","journal-title":"Anim. Reprod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1095\/biolreprod.103.020651","article-title":"Osteopontin: Roles in Implantation and Placentation","volume":"69","author":"Johnson","year":"2003","journal-title":"Biol. Reprod."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1016\/j.theriogenology.2005.08.021","article-title":"Defining Postpartum Uterine Disease in Cattle","volume":"65","author":"Sheldon","year":"2006","journal-title":"Theriogenology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1093\/biolre\/ioae149","article-title":"Insight into the Post-Translational Modifications in Pregnancy and Related Complications","volume":"112","author":"Yin","year":"2025","journal-title":"Biol. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1051\/vetres:2002015","article-title":"Evaluation of L-Selectin Expression and Assessment of Protein Tyrosine Phosphorylation in Bovine Polymorphonuclear Neutrophil Leukocytes around Parturition","volume":"33","author":"Monfardini","year":"2002","journal-title":"Vet. Res."},{"key":"ref_17","first-page":"125","article-title":"Phosphorylation of Tyrosine in Cultured Human Placenta","volume":"804","author":"Galski","year":"1984","journal-title":"BBA\u2014Mol. Cell Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wang, F., Wang, L., Shi, Z., and Liang, G. (2013). Comparative N-Glycoproteomic and Phosphoproteomic Profiling of Human Placental Plasma Membrane between Normal and Preeclampsia Pregnancies with High-Resolution Mass Spectrometry. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080480"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1292\/jvms.54.81","article-title":"Signal Transduction Systems Mediated by Protein Kinase-C and Estradiol Receptors in Cow Placenta and Caruncle","volume":"54","author":"Katoh","year":"1992","journal-title":"J. Vet. Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1046\/j.1439-0442.2001.00352.x","article-title":"Protein Peroxidation Processes in Bovine Retained and Not-Retained Placenta","volume":"48","author":"Kankofer","year":"2001","journal-title":"J. Vet. Med. Ser. A"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1046\/j.1439-0531.2001.d01-38.x","article-title":"Non-Enzymatic Antioxidative Defence Mechanisms against Reactive Oxygen Species in Bovine-Retained and Not-Retained Placenta: Vitamin C and Glutathione","volume":"36","author":"Kankofer","year":"2001","journal-title":"Reprod. Domest. Anim."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8751","DOI":"10.1038\/s41467-024-52742-3","article-title":"Strain-Dependent Glutathionylation of Fibronectin Fibers Impacts Mechano-Chemical Behavior and Primes an Integrin Switch","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"C27","DOI":"10.1152\/ajpcell.00379.2024","article-title":"B3-Adrenergic Agonist Counters Oxidative Stress and Na1 -K1 Pump Inhibitory S-Glutathionylation of Placental Cells: Implications for Preeclampsia","volume":"328","author":"Liu","year":"2025","journal-title":"Am. J. Physiol.\u2014Cell Physiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1111\/rda.13543","article-title":"Patterns of Protein Glycosylation in Bovine Placentomes as a Function of Gestational Age and in Retained versus Non-Retained Placenta","volume":"54","author":"Wawrzykowski","year":"2019","journal-title":"Reprod. Domest. Anim."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1111\/rda.13747","article-title":"Activity of Selected Glycosidases and Availability of Their Substrates in Bovine Placenta during Pregnancy and Parturition with and without Retained Foetal Membranes","volume":"55","author":"Wawrzykowski","year":"2020","journal-title":"Reprod. Domest. Anim."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.theriogenology.2017.09.031","article-title":"Pregnancy-Associated Glycoproteins in Cows with Retained Fetal Membranes","volume":"105","author":"Hooshmandabbasi","year":"2018","journal-title":"Theriogenology"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Krog, C.H., Agerholm, J.S., and Nielsen, S.S. (2018). Fetal Age Assessment for Holstein Cattle. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0207682"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1093\/biomet\/52.3-4.591","article-title":"An Analysis of Variance Test for Normality (Complete Samples)","volume":"52","author":"Shapiro","year":"1965","journal-title":"Biometrika"},{"key":"ref_29","unstructured":"Levene, H. (1960). Robust Tests for Equality of Variances. Contributions to Probability and Statistics: Essays in Honor of Harold Hotelling, Stanford University Press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1080\/01621459.1952.10483441","article-title":"Use of Ranks in One-Criterion Variance Analysis","volume":"47","author":"Kruskal","year":"1952","journal-title":"J. Am. Stat. Assoc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1214\/aoms\/1177730491","article-title":"On a Test of Whether One of Two Random Variables Is Stochastically Larger than the Other","volume":"18","author":"Mann","year":"1947","journal-title":"Ann. Math. Stat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1097\/00001648-199001000-00010","article-title":"No Adjustments Are Needed for Multiple Comparisons","volume":"1","author":"Rothman","year":"1990","journal-title":"Epidemiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1093\/jnen\/nly003","article-title":"Cleaved \u03b2-Actin May Contribute to DNA Fragmentation Following Very Brief Focal Cerebral Ischemia","volume":"77","author":"Zhouheng","year":"2018","journal-title":"J. Neuropathol. Exp. Neurol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/22\/10984\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,15]],"date-time":"2025-11-15T05:23:03Z","timestamp":1763184183000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/22\/10984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,13]]},"references-count":33,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["ijms262210984"],"URL":"https:\/\/doi.org\/10.3390\/ijms262210984","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2025,11,13]]}}}