{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T21:38:19Z","timestamp":1783114699249,"version":"3.54.6"},"reference-count":36,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,16]],"date-time":"2025-11-16T00:00:00Z","timestamp":1763251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dengue virus (DENV) affects not only peripheral immune cells but also hematopoietic progenitors in the bone marrow, particularly megakaryocytic precursors, which contribute to the thrombocytopenia characteristic of the disease. In this study, we evaluated the relationship between the differentiation status of the megakaryocytic lineage and its permissiveness and antiviral response to DENV. Our results demonstrate that the erythroid\u2013megakaryocytic precursor (K562 cells) was more permissive to DENV infection than megakaryoblasts, as evidenced by immunofluorescence, flow cytometry, and quantification of viral particles. The antiviral response in K562 cells peaked at three days post-infection, with maximal expression of genes associated with the type I interferon (IFN-I) pathway. In vitro-induced differentiation of K562 cells reduced the initial susceptibility to DENV and enhanced the expression of Toll-like receptor 3 (TLR3) and the type I interferon receptor (IFNAR1), accelerating and intensifying IFN-\u03b2 secretion, and increasing the expression of OAS2 and IRF3. Furthermore, pretreatment of K562 cells with recombinant IFN-\u03b2 significantly reduced viral replication from the first day post-infection. Collectively, these findings demonstrate for the first time that the differentiation status of erythroid\u2013megakaryocytic progenitor critically shapes their antiviral response and underscore the central role of IFN-\u03b2 in the early restriction of DENV infection.<\/jats:p>","DOI":"10.3390\/ijms262211081","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T10:24:58Z","timestamp":1763375098000},"page":"11081","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Megakaryocytic Differentiation Regulates the Permissiveness and Antiviral Response of the Megakaryocytic Erythroid Progenitor to Dengue Virus"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4535-5262","authenticated-orcid":false,"given":"Diego Sait","family":"Cruz-Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Departamento de Biomedicina Experimental, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma \u201cBenito Ju\u00e1rez\u201d de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7954-3910","authenticated-orcid":false,"given":"Francisco Javier","family":"S\u00e1nchez-Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Secretaria de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma Benito Ju\u00e1rez de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4604-0751","authenticated-orcid":false,"given":"Marymar","family":"Cruz-Cruz","sequence":"additional","affiliation":[{"name":"Secretaria de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma Benito Ju\u00e1rez de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2907-1440","authenticated-orcid":false,"given":"Dar\u00edo de Jes\u00fas","family":"Guill\u00e9n-Morales","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Experimental, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma \u201cBenito Ju\u00e1rez\u201d de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7862-2224","authenticated-orcid":false,"given":"Martha Cristina","family":"Castillo-Soriano","sequence":"additional","affiliation":[{"name":"Departamento de Biomedicina Experimental, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma \u201cBenito Ju\u00e1rez\u201d de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Cruz-Altamirano","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma Benito Ju\u00e1rez de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8532-6717","authenticated-orcid":false,"given":"Juan","family":"Alpuche","sequence":"additional","affiliation":[{"name":"Laboratorio de Bioqu\u00edmica, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma Benito Ju\u00e1rez de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7368-6432","authenticated-orcid":false,"given":"Jos\u00e9","family":"Bustos-Arriaga","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular e Inmunolog\u00eda de Arbovirus, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Estado de M\u00e9xico 54090, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda de Los \u00c1ngeles","family":"Romero-Tlalolini","sequence":"additional","affiliation":[{"name":"Secretaria de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n, Facultad de Medicina y Cirug\u00eda, Universidad Aut\u00f3noma Benito Ju\u00e1rez de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2853-4891","authenticated-orcid":false,"given":"Honorio","family":"Torres-Aguilar","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Aut\u00f3noma \u201cBenito Ju\u00e1rez\u201d de Oaxaca, Oaxaca 68120, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Rodr\u00edguez-Alba","sequence":"additional","affiliation":[{"name":"Unidad de Neuroinmunolog\u00eda y Neurooncolog\u00eda, Instituto Nacional de Neurolog\u00eda y Neurocirug\u00eda, Ciudad de M\u00e9xico 14269, 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