{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:38:13Z","timestamp":1778643493411,"version":"3.51.4"},"reference-count":124,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Invest in ME Research","award":["1153730"],"award-info":[{"award-number":["1153730"]}]},{"name":"BBSRC Institute Strategic Programme","award":["BB\/X011054\/1"],"award-info":[{"award-number":["BB\/X011054\/1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS) is a debilitating chronic condition without a definitive aetiology, no reliable diagnostic test, and no proven effective treatment. Despite most patients reporting a post-viral onset of illness, findings to date are conflicting on whether a single virus or multiple viral triggers are involved. Most studies to date have focused on detecting viruses in blood and circulating immune cells with relatively few investigating the presence of viruses in mucosal sites. In this review, we propose that this represents a critical gap in understanding the pathophysiology of ME\/CFS knowledge, as mucosal tissues are primary entry points for most pathogens and often serve as reservoirs where viruses may persist. Consequently, they represent ideal niches for identifying persistent infections in ME\/CFS. Emerging evidence from saliva and other mucosal samples in ME\/CFS patients is consistent with this proposal and that latent viruses can persist and periodically reactivate in mucosal tissues from where they can potentially contribute to immune dysregulation, chronic inflammation, and increased symptom severity that defines ME\/CFS.<\/jats:p>","DOI":"10.3390\/ijms262211161","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T08:50:07Z","timestamp":1763542207000},"page":"11161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mucosal Viruses in Myalgic Encephalomyelitis\/Chronic Fatigue Syndrome: A Missing Piece of the Puzzle?"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3778-4975","authenticated-orcid":false,"given":"Krishani Dinali","family":"Perera","sequence":"first","affiliation":[{"name":"Food, Microbiome and Health Research Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige","family":"Cameron","sequence":"additional","affiliation":[{"name":"Food, Microbiome and Health Research Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tayyibah","family":"Sarwar","sequence":"additional","affiliation":[{"name":"Food, Microbiome and Health Research Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2383-9701","authenticated-orcid":false,"given":"Simon R.","family":"Carding","sequence":"additional","affiliation":[{"name":"Food, Microbiome and Health Research Programme, Quadram Institute Bioscience, Norwich Research Park, Norwich NR4 7UQ, UK"},{"name":"Norwich Medical School, University East Anglia, Norwich NR4 7UQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1186\/s12967-025-06281-0","article-title":"Letter: Time to correct the record on the global burden of myalgic encephalomyelitis\/chronic fatigue syndrome (ME\/CFS)","volume":"23","author":"Vardaman","year":"2025","journal-title":"J. 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