{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T16:37:28Z","timestamp":1789576648527,"version":"build-2803163510"},"reference-count":109,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007771","name":"Medical University of Silesia","doi-asserted-by":"publisher","award":["BNW-1-075\/N\/4\/K"],"award-info":[{"award-number":["BNW-1-075\/N\/4\/K"]}],"id":[{"id":"10.13039\/100007771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Root cementum is a specialized connective tissue with a critical role in tooth function and periodontal health. This narrative review aims to consolidate current knowledge regarding the influence of cementum on the pathophysiology of periodontitis and root caries, its remodeling during orthodontic tooth movement, its significance in regenerative strategies and implantology, along with the effect of selected stimulants. A comprehensive literature search was conducted across PubMed, Embase, and Scopus databases for articles fulfilling the inclusion criteria. The analysis revealed that cementum\u2019s unique biological characteristics are fundamental to the success of periodontal regeneration, with biomimetic approaches showing promise for enhancing osseointegration of dental implants. Furthermore, the tissue is highly susceptible to mechanical forces, leading to adverse effects such as root resorption during orthodontic treatment. Its composition also renders it vulnerable to demineralization from root caries and damage from external stimulants. In conclusion, a profound understanding of the intricate biology of root cementum is essential for developing safer and more effective treatment modalities. The findings strongly support the development of targeted, cementum-oriented interventions and preventive strategies, thereby improving long-term success in restorative, periodontal, and orthodontic treatment.<\/jats:p>","DOI":"10.3390\/ijms262211178","type":"journal-article","created":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T12:55:31Z","timestamp":1763556931000},"page":"11178","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Root Cementum Molecular Structure and Its Role in Maintaining Oral Health\u2014Systematic Review"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-071X","authenticated-orcid":false,"given":"Katarzyna","family":"Janik","sequence":"first","affiliation":[{"name":"Department of Dental Propedeutics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 15 Poniatowskiego Street, 40-055 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0584-968X","authenticated-orcid":false,"given":"Ma\u0142gorzata","family":"Skucha-Nowak","sequence":"additional","affiliation":[{"name":"Department of Dental Propedeutics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 15 Poniatowskiego Street, 40-055 Katowice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1600-0757.1997.tb00095.x","article-title":"Dental cementum: The dynamic tissue covering of the root","volume":"13","author":"Bosshardt","year":"1997","journal-title":"Periodontol. 2000"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"102913","DOI":"10.1016\/j.micron.2020.102913","article-title":"Teeth resorption at cement\u2013enamel junction (CEJ)\u2014Microscopy analysis","volume":"137","author":"Metwally","year":"2020","journal-title":"Micron"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/BF02012780","article-title":"A preliminary characterization of human cementum collagen","volume":"23","author":"Christner","year":"1977","journal-title":"Calcif. Tissue Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jdsr.2016.04.002","article-title":"Histology of human cementum: Its structure, function, and development","volume":"52","author":"Yamamoto","year":"2016","journal-title":"Jpn. Dent. Sci. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1177\/00220345221100794","article-title":"The Bone Sialoprotein RGD Domain Modulates and Maintains Periodontal Development","volume":"101","author":"Nagasaki","year":"2022","journal-title":"J. Dent. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1093\/jbmr\/zjaf062","article-title":"Functions of secretory calcium-binding phosphoproteins in dental mineralization","volume":"40","author":"Chun","year":"2025","journal-title":"J. Bone Miner. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0003-9969(98)00001-6","article-title":"Immunolocalization of glycosaminoglycans in ageing, healthy and periodontally diseased human cementum","volume":"43","author":"Ababneh","year":"1998","journal-title":"Arch. Oral Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1600-0765.1999.tb02227.x","article-title":"The proteoglycans of human cementum: Immunohistochemical localization in healthy, periodontally involved and ageing teeth","volume":"34","author":"Ababneh","year":"1999","journal-title":"J. Periodontal Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0070-2153(06)78003-6","article-title":"Advances in Defining Regulators of Cementum Development and Periodontal Regeneration","volume":"Volume 78","author":"Foster","year":"2007","journal-title":"Current Topics in Developmental Biology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"103485","DOI":"10.1016\/j.micron.2023.103485","article-title":"Elemental mapping of human teeth enamel, dentine and cementum in view of their microstructure","volume":"172","author":"Skic","year":"2023","journal-title":"Micron"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0074-7696(08)62074-4","article-title":"Biological Problems of Regenerative Cementogenesis: Synthesis and Attachment of Collagenous Matrices on Growing and Established Root Surfaces","volume":"Volume 142","author":"Schroeder","year":"1992","journal-title":"International Review of Cytology"},{"key":"ref_12","first-page":"550","article-title":"Human cellular mixed stratified cementum: A tissue with alternating layers of acellular extrinsic- and cellular intrinsic fiber cementum","volume":"103","author":"Schroeder","year":"1993","journal-title":"Schweiz. Monatsschrift Zahnmed."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1177\/00220345200020010901","article-title":"Concerning Human Cementum","volume":"2","year":"1920","journal-title":"J. Dent. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/S1079-2104(05)80106-4","article-title":"Intermediate cementum","volume":"79","author":"Harrison","year":"1995","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2334\/josnusd.49.1","article-title":"Physiologic root resorption in primary teeth: Molecular and histological events","volume":"49","year":"2007","journal-title":"J. Oral Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1111\/jre.12808","article-title":"Comparative proteomic analysis of dental cementum from deciduous and permanent teeth","volume":"56","author":"Giovani","year":"2021","journal-title":"J. Periodontal Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/prd.12062","article-title":"Cementum proteins: Role in cementogenesis, biomineralization, periodontium formation and regeneration","volume":"67","author":"Arzate","year":"2015","journal-title":"Periodontol. 2000"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1111\/jre.13041","article-title":"Polycaprolactone scaffolds as a biomaterial for cementoblast delivery: An in vitro study","volume":"57","author":"Abdalla","year":"2022","journal-title":"J. Periodontal Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"107726","DOI":"10.1016\/j.jsb.2021.107726","article-title":"Cementum thickening leads to lower whole tooth mobility and reduced root stresses: An in silico study on aging effects during mastication","volume":"213","author":"Morgenthal","year":"2021","journal-title":"J. Struct. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1600-0757.1993.tb00230.x","article-title":"Tissues and cells of the periodontium","volume":"3","author":"Hassell","year":"1993","journal-title":"Periodontol. 2000"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"569","DOI":"10.14219\/jada.archive.1971.0101","article-title":"Cementogenic Repair of Root Fractures","volume":"82","author":"Michanowicz","year":"1971","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_22","first-page":"414","article-title":"Intraalveolar root fractures: Radiographic and histologic study of 50 cases","volume":"25","author":"Andreasen","year":"1967","journal-title":"J. Oral Surg. Am. Dent. Assoc. 1965"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1111\/iej.13611","article-title":"Cemental tear: Literature review, proposed classification and recommendations for treatment","volume":"54","author":"Lee","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"S82","DOI":"10.1111\/adj.12844","article-title":"Cemental tear: An overlooked finding associated with rapid periodontal destruction. A case series","volume":"66","author":"Pedercini","year":"2021","journal-title":"Aust. Dent. J."},{"key":"ref_25","first-page":"61","article-title":"Diagnosis and treatment of cemental tear: A case series","volume":"73","author":"Mathew","year":"2025","journal-title":"Gen. Dent."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Aizenbud, I., Wilensky, A., and Almoznino, G. (2023). Periodontal Disease and Its Association with Metabolic Syndrome\u2014A Comprehensive Review. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241613011"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S1","DOI":"10.1002\/JPER.18-0157","article-title":"A new classification scheme for periodontal and peri-implant diseases and conditions\u2014Introduction and key changes from the 1999 classification","volume":"89","author":"Caton","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12616","article-title":"Periodontal disease: A systemic condition","volume":"96","author":"Villoria","year":"2024","journal-title":"Periodontol. 2000"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/prd.12430","article-title":"Interconnection of periodontal disease and comorbidities: Evidence, mechanisms, and implications","volume":"89","author":"Hajishengallis","year":"2022","journal-title":"Periodontol. 2000"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12344","article-title":"Clinical and public health implications of periodontal and systemic diseases: An overview","volume":"83","author":"Genco","year":"2020","journal-title":"Periodontol. 2000"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2320120","DOI":"10.1080\/13814788.2024.2320120","article-title":"Periodontal diseases and cardiovascular diseases, diabetes, and respiratory diseases: Summary of the consensus report by the European Federation of Periodontology and WONCA Europe","volume":"30","author":"Herrera","year":"2024","journal-title":"Eur. J. Gen. Pract."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13686","DOI":"10.1038\/s41598-021-93062-6","article-title":"Bidirectional association between periodontal disease and diabetes mellitus: A systematic review and meta-analysis of cohort studies","volume":"11","author":"Barbaresko","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Vinel, A., Al Halabi, A., Roumi, S., Le Neindre, H., Millavet, P., Simon, M., Cuny, C., Barthet, J.S., Barthet, P., and Laurencin-Dalicieux, S. (2022). Non-surgical Periodontal Treatment: SRP and Innovative Therapeutic Approaches. Advances in Experimental Medicine and Biology, Springer International Publishing.","DOI":"10.1007\/978-3-030-96881-6_16"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/idj.12630","article-title":"Current Concepts in the Management of Periodontitis","volume":"71","author":"Kwon","year":"2021","journal-title":"Int. Dent. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/prd.12332","article-title":"Contemporary practices for mechanical oral hygiene to prevent periodontal disease","volume":"84","author":"Graetz","year":"2020","journal-title":"Periodontol. 2000"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"560","DOI":"10.4103\/0972-124X.142440","article-title":"Evaluation of the nanostructure of cervical third cementum in health and chronic periodontitis: An in vitro study","volume":"18","author":"Sundaram","year":"2014","journal-title":"J. Indian Soc. Periodontol."},{"key":"ref_38","first-page":"22808000231211416","article-title":"Polycaprolactone-based scaffolds for guided tissue regeneration in periodontal therapy: A systematic review","volume":"21","author":"Antunovic","year":"2023","journal-title":"J. Appl. Biomater. Funct. Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/jre.12130","article-title":"Comparison of treatment effects of guided tissue regeneration on infrabony lesions between animal and human studies: A systematic review and meta-analysis","volume":"49","author":"Yen","year":"2014","journal-title":"J. Periodontal Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"S77","DOI":"10.1902\/jop.2015.130685","article-title":"Periodontal Regeneration\u2014Intrabony Defects: A Systematic Review From the AAP Regeneration Workshop","volume":"86","author":"Kao","year":"2015","journal-title":"J. Periodontol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Liu, J., Dai, Q., and Weir, M.D. (2020). Biocompatible Nanocomposite Enhanced Osteogenic and Cementogenic Differentiation of Periodontal Ligament Stem Cells In Vitro for Periodontal Regeneration. Materials, 13.","DOI":"10.3390\/ma13214951"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1186\/s12903-020-01124-4","article-title":"Development and application of a 3D periodontal in vitro model for the evaluation of fibrillar biomaterials","volume":"20","author":"Koch","year":"2020","journal-title":"BMC Oral Health"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"559494","DOI":"10.3389\/fbioe.2020.559494","article-title":"An in-vivo Intraoral Defect Model for Assessing the Use of P11-4 Self-Assembling Peptide in Periodontal Regeneration","volume":"8","author":"Davies","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1601251","DOI":"10.1002\/adhm.201601251","article-title":"Tri-Layered Nanocomposite Hydrogel Scaffold for the Concurrent Regeneration of Cementum, Periodontal Ligament, and Alveolar Bone","volume":"6","author":"Sowmya","year":"2017","journal-title":"Adv. Healthc. Mater."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1016\/j.ijbiomac.2020.10.106","article-title":"Weissella cibaria EIR\/P2-derived exopolysaccharide: A novel alternative to conventional biomaterials targeting periodontal regeneration","volume":"165","author":"Kibar","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s10856-024-06840-9","article-title":"Comparison of different graft materials on the proliferation, mineralization and mineralized tissue-related gene expressions of cementoblasts","volume":"36","author":"Hakki","year":"2025","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3390\/encyclopedia1010011","article-title":"Periodontal Regeneration","volume":"1","author":"Mancini","year":"2021","journal-title":"Encyclopedia"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Narita, L.E., Mester, A., Onisor, F., Bran, S., Onicas, M.I., and Voina-Tonea, A. (2021). The Outcomes of Enamel Matrix Derivative on Periodontal Regeneration under Diabetic Conditions. Medicina, 57.","DOI":"10.3390\/medicina57101071"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Miron, R.J., Shirakata, Y., Ahmad, P., Romandini, M., Estrin, N.E., Farshidfar, N., Bosshardt, D.D., and Sculean, A. (2025). 30\u2009years of enamel matrix derivative: Mimicking tooth development as a clinical concept. Periodontol. 2000.","DOI":"10.1111\/prd.12635"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"310","DOI":"10.2174\/1389450121999201105152639","article-title":"The Role of Type 2 Fibroblast Growth Factor in Periodontal Therapy","volume":"22","author":"Mohammadipour","year":"2021","journal-title":"Curr. Drug Targets"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"20406223241302707","DOI":"10.1177\/20406223241302707","article-title":"Latest update on the use of recombinant growth factors for periodontal regeneration: Existing evidence and clinical applications","volume":"15","author":"Khehra","year":"2024","journal-title":"Ther. Adv. Chronic Dis."},{"key":"ref_52","first-page":"90","article-title":"Histologic evidence of the effect of fibroblast growth factor 2 on periodontal regeneration: A scoping review of animal studies","volume":"17","author":"Taghipour","year":"2025","journal-title":"J. Adv. Periodontol. Implant Dent."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.ajodo.2025.04.023","article-title":"Fibroblast growth factor 2 stimulates differentiation of mechanically-stressed human periodontal ligament fibroblasts into cementoblasts","volume":"168","author":"Rikimaru","year":"2025","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e23514","DOI":"10.1002\/dvg.23514","article-title":"Cementum is key to periodontal tissue regeneration: A review on apatite microstructures for creation of novel cementum-based dental implants","volume":"61","author":"Saito","year":"2023","journal-title":"Genesis"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"341","DOI":"10.3389\/fbioe.2020.00341","article-title":"The Construction of Biomimetic Cementum Through a Combination of Bioskiving and Fluorine-Containing Biomineralization","volume":"8","author":"Yang","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3","DOI":"10.5051\/jpis.2010.40.1.3","article-title":"Histological characteristics of newly formed cementum in surgically created one-wall intrabony defects in a canine model","volume":"40","author":"Park","year":"2010","journal-title":"J. Periodontal Implant Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.34172\/joddd.2022.001","article-title":"Biomaterials and biotechnology for periodontal tissue regeneration: Recent advances and perspectives","volume":"16","author":"Deng","year":"2022","journal-title":"J. Dent. Res. Dent. Clin. Dent. Prospect."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1186\/s13287-016-0417-x","article-title":"Composite cell sheet for periodontal regeneration: Crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration","volume":"7","author":"Zhang","year":"2016","journal-title":"Stem Cell Res. Ther."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.1016\/j.dental.2019.05.016","article-title":"Treated dentin matrix particles combined with dental follicle cell sheet stimulate periodontal regeneration","volume":"35","author":"Yang","year":"2019","journal-title":"Dent. Mater."},{"key":"ref_60","first-page":"2280800019900094","article-title":"A simvastatin-releasing scaffold with periodontal ligament stem cell sheets for periodontal regeneration","volume":"18","author":"Zhao","year":"2020","journal-title":"J. Appl. Biomater. Funct. Mater."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"27703","DOI":"10.1021\/acsami.2c06679","article-title":"Titanium Nanosurface with a Biomimetic Physical Microenvironment to Induce Endogenous Regeneration of the Periodontium","volume":"14","author":"Yamada","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"242","DOI":"10.5051\/jpis.2006080304","article-title":"Bio-hybrid dental implants prepared using stem cells with \u03b2-TCP-coated titanium and zirconia","volume":"52","author":"Safi","year":"2022","journal-title":"J. Periodontal Implant Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1111\/jre.13295","article-title":"Cementum and enamel surface mimicry influences soft tissue cell behavior","volume":"60","author":"Bellon","year":"2025","journal-title":"J. Periodontal Res."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kim, H.-Y., Im, H.-Y., Chang, H.-K., Jeong, H.-D., Park, J.-H., Kim, H.-I., Yi, H.-S., and Kim, Y.-S. (2023). Correlation between Collagen Type I\/III Ratio and Scar Formation in Patients Undergoing Immediate Reconstruction with the Round Block Technique after Breast-Conserving Surgery. Biomedicines, 11.","DOI":"10.3390\/biomedicines11041089"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"116139","DOI":"10.1016\/j.bone.2021.116139","article-title":"Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature","volume":"153","author":"Chavez","year":"2021","journal-title":"Bone"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"416","DOI":"10.2319\/071116-541.1","article-title":"Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement","volume":"87","author":"Matsuzawa","year":"2017","journal-title":"Angle Orthod."},{"key":"ref_67","first-page":"3174","article-title":"Establishment of in vitro three-dimensional cementocyte differentiation scaffolds to study orthodontic root resorption","volume":"20","author":"Wei","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3892\/mmr.2022.12744","article-title":"Dynamic alternations of RANKL\/OPG ratio expressed by cementocytes in response to orthodontic-induced external apical root resorption in a rat model","volume":"26","author":"Wei","year":"2022","journal-title":"Mol. Med. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/ger.12511","article-title":"The burden of root caries: Updated perspectives and advances on management strategies","volume":"38","author":"AlQranei","year":"2021","journal-title":"Gerodontology"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e20240013","DOI":"10.1590\/1678-7757-2024-0013","article-title":"The action of microbial collagenases in dentinal matrix degradation in root caries and potential strategies for its management: A comprehensive state-of-the-art review","volume":"32","author":"Barbosa","year":"2024","journal-title":"J. Appl. Oral Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1159\/000447309","article-title":"Ecological Hypothesis of Dentin and Root Caries","volume":"50","author":"Takahashi","year":"2016","journal-title":"Caries Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1159\/000510843","article-title":"How to Intervene in the Caries Process in Older Adults: A Joint ORCA and EFCD Expert Delphi Consensus Statement","volume":"54","author":"Paris","year":"2020","journal-title":"Caries Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1159\/000479304","article-title":"Root Surface Biofilms and Caries","volume":"26","author":"Do","year":"2017","journal-title":"Monogr. Oral Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1159\/000363582","article-title":"Matrix Metalloproteinases and Other Matrix Proteinases in Relation to Cariology: The Era of \u2018Dentin Degradomics\u2019","volume":"49","author":"Buzalaf","year":"2015","journal-title":"Caries Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1278754","DOI":"10.3389\/fcimb.2023.1278754","article-title":"Presence of host and bacterial-derived collagenolytic proteases in carious dentin: A systematic review of ex vivo studies","volume":"13","author":"Barbosa","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1111\/ger.12409","article-title":"Atraumatic vs conventional restorative treatment for root caries lesions in older patients: Meta- and trial sequential analysis","volume":"36","author":"McKenna","year":"2019","journal-title":"Gerodontology."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1159\/000501588","article-title":"How to Intervene in the Root Caries Process? Systematic Review and Meta-Analyses","volume":"53","author":"Machiulskiene","year":"2019","journal-title":"Caries Res."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e941","DOI":"10.1002\/cre2.941","article-title":"Evaluating the Effects of Cigarette Smoking and Heated Tobacco Products on Hard Dental Tissues: A Comparative Histological and Colorimetric Analysis","volume":"10","author":"Makkeyah","year":"2024","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Onor, I.O., Stirling, D.L., Williams, S.R., Bediako, D., Borghol, A., Harris, M.B., Darensburg, T.B., Clay, S.D., Okpechi, S.C., and Sarpong, D.F. (2017). Clinical Effects of Cigarette Smoking: Epidemiologic Impact and Review of Pharmacotherapy Options. Int. J. Environ. Res. Public Health, 14.","DOI":"10.3390\/ijerph14101147"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1093\/toxres\/tfaa031","article-title":"The effect of different tobacco tar levels on DNA damage in cigarette smoking subjects","volume":"9","author":"Zhao","year":"2020","journal-title":"Toxicol. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1002\/cre2.683","article-title":"Effect of tobacco and nicotine in causing staining of dental hard tissues and dental materials: A systematic review and meta-analysis","volume":"9","author":"Karanjkar","year":"2023","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1002\/cre2.764","article-title":"Effects of aerosols from heated tobacco products with flavors on the discoloration of bovine tooth enamel","volume":"9","author":"Kurachi","year":"2023","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Mori, Y., Tanaka, M., Kozai, H., Aoyama, Y., Shigeno, Y., Hotta, K., Aoike, M., Kawamura, H., Tsurudome, M., and Ito, M. (2022). Effects of Heat-Not-Burn Cigarette Smoking on the Secretion of Saliva and Its Innate Immune System Components. Healthcare, 11.","DOI":"10.3390\/healthcare11010132"},{"key":"ref_84","first-page":"211","article-title":"Association of Xerostomia and Assessment of Salivary Flow Using Modified Schirmer Test among Smokers and Healthy Individuals: A Preliminutesary Study","volume":"8","author":"Dyasanoor","year":"2014","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Sever, E., Bo\u017eac, E., Saltovi\u0107, E., Simoni\u0107-Kocijan, S., Brumini, M., and Gla\u017ear, I. (2023). Impact of the Tobacco Heating System and Cigarette Smoking on the Oral Cavity: A Pilot Study. Dent. J., 11.","DOI":"10.3390\/dj11110251"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.4317\/jced.55910","article-title":"A time based objective evaluation of the erosive effects of various beverages on enamel and cementum of deciduous and permanent teeth","volume":"12","author":"Rajeev","year":"2020","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"e242436","DOI":"10.1590\/2177-6709.29.6.e242436.oar","article-title":"Changes in salivary biomarkers of pain, anxiety, stress, and inflammation related to tooth movement during orthodontic treatment: A systematic review","volume":"29","author":"Rodrigues","year":"2024","journal-title":"Dent. Press J. Orthod."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ajodo.2012.08.026","article-title":"Time-related changes in salivary levels of the osteotropic factors sRANKL and OPG through orthodontic tooth movement","volume":"143","year":"2013","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s00774-020-01178-y","article-title":"Regulation of T cell-associated tissues and T cell activation by RANKL-RANK-OPG","volume":"39","author":"Walsh","year":"2021","journal-title":"J. Bone Miner. Metab."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"637606","DOI":"10.3389\/fphys.2021.637606","article-title":"Impact of Orthodontic Forces on Plasma Levels of Markers of Bone Turnover and Inflammation in a Rat Model of Buccal Expansion","volume":"12","author":"Danz","year":"2021","journal-title":"Front. Physiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1006\/bbrc.2000.3379","article-title":"Protein Expression and Functional Difference of Membrane-Bound and Soluble Receptor Activator of NF-\u03baB Ligand: Modulation of the Expression by Osteotropic Factors and Cytokines","volume":"275","author":"Nakashima","year":"2000","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1186\/s12903-022-02327-7","article-title":"Mechanical force regulates root resorption in rats through RANKL and OPG","volume":"22","author":"Zhou","year":"2022","journal-title":"BMC Oral Health"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.1007\/s11033-022-08190-7","article-title":"Bone cells and their role in physiological remodeling","volume":"50","author":"Maciel","year":"2023","journal-title":"Mol. Biol. Rep."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1177\/0004563218759371","article-title":"The bone remodelling cycle","volume":"55","author":"Kenkre","year":"2018","journal-title":"Ann. Clin. Biochem."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"105873","DOI":"10.1155\/2013\/105873","article-title":"Biomarkers of Periodontal Tissue Remodeling during Orthodontic Tooth Movement in Mice and Men: Overview and Clinical Relevance","volume":"2013","author":"Cappabianca","year":"2013","journal-title":"Sci. World J."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Yamaguchi, M., and Fukasawa, S. (2021). Is Inflammation a Friend or Foe for Orthodontic Treatment?: Inflammation in Orthodontically Induced Inflammatory Root Resorption and Accelerating Tooth Movement. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052388"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"116961","DOI":"10.1016\/j.bone.2023.116961","article-title":"Functional defects in cementoblasts with disrupted bone sialoprotein functional domains, in vitro","volume":"179","author":"Chavez","year":"2024","journal-title":"Bone"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Dab, S., Abdelhay, N., Figueredo, C.A., Ganatra, S., and Gibson, M.P. (2022). Characterization of SIBLING Proteins in the Mineralized Tissues. Dent. J., 10.","DOI":"10.3390\/dj10080144"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.bone.2015.05.007","article-title":"Mineralization defects in cementum and craniofacial bone from loss of bone sialoprotein","volume":"78","author":"Foster","year":"2015","journal-title":"Bone"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1177\/0022034515592581","article-title":"Mechanical Forces Exacerbate Periodontal Defects in Bsp-null Mice","volume":"94","author":"Soenjaya","year":"2015","journal-title":"J. Dent. Res."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.bone.2017.08.027","article-title":"Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis","volume":"105","author":"Ao","year":"2017","journal-title":"Bone"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/03008209509016974","article-title":"Role of Two Mineral-Associated Adhesion Molecules, Osteopontin and Bone Sialoprotein, during Cementogenesis","volume":"33","author":"Macneil","year":"1995","journal-title":"Connect. Tissue Res."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.bone.2017.12.004","article-title":"Osteopontin regulates dentin and alveolar bone development and mineralization","volume":"107","author":"Foster","year":"2018","journal-title":"Bone"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"605996","DOI":"10.3389\/fcell.2020.605996","article-title":"Aging and Senescence of Dental Pulp and Hard Tissues of the Tooth","volume":"8","author":"Maeda","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1016\/j.identj.2022.05.008","article-title":"Prevention of Oral Functional Decline","volume":"72","author":"Ogawa","year":"2022","journal-title":"Int. Dent. J."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jdent.2019.03.003","article-title":"An RCT of atraumatic restorative treatment for older adults: 5 year results","volume":"83","author":"McKenna","year":"2019","journal-title":"J. Dent."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1016\/j.jdent.2014.08.012","article-title":"Tooth loss and its association with dietary intake and diet quality in American adults","volume":"42","author":"Zhu","year":"2014","journal-title":"J. Dent."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s12903-022-02210-5","article-title":"Tooth retention, health, and quality of life in older adults: A scoping review","volume":"22","author":"Atanda","year":"2022","journal-title":"BMC Oral Health"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/ger.12606","article-title":"Philosophy of Oral Hypofunction","volume":"39","author":"Minakuchi","year":"2022","journal-title":"Gerodontology"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/22\/11178\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T12:55:59Z","timestamp":1763556959000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/26\/22\/11178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,19]]},"references-count":109,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["ijms262211178"],"URL":"https:\/\/doi.org\/10.3390\/ijms262211178","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,19]]}}}