{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:09:09Z","timestamp":1763975349031,"version":"3.45.0"},"reference-count":25,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Strategic Programme Excellence Initiative at Jagiellonian University","award":["U1C\/W42\/NO\/28.17"],"award-info":[{"award-number":["U1C\/W42\/NO\/28.17"]}]},{"name":"Polish Ministry of Science and Education","award":["N42\/DBS\/000435"],"award-info":[{"award-number":["N42\/DBS\/000435"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The main aim of this study was to evaluate the effect of space-like environment on the chemopreventive activity of Brassica sprouts against thyroid cancer cells in vitro. For this purpose the sprouts of broccoli, kale, kohlrabi, and Brussels sprouts were cultivated in darkness and in microgravity for 5\u20137 days. Then, the sprouts\u2019 extracts were examined for cytotoxic and antiproliferative activity against thyroid cancer and normal cells. The tested microgravity environment stimulated the cytotoxic activity of kohlrabi sprouts, causing approximately 50% reduction in thyroid cancer cells\u2019 viability, while at the same time increasing the viability of normal thyroid cells. Broccoli sprouts showed the strongest antiproliferative activity against normal thyroid cells, with the best effect visible for darkness conditions, which may contribute to the reduction of thyroid hyperplasia. Microgravity and darkness significantly enhanced the antiproliferative activity of kale, especially in 7-day-old sprouts (inhibition approximately 90%). The tested conditions also increased the antiproliferative activity of kohlrabi sprouts, but in the case of Brussels sprouts the effect was unfavorable. The study showed that microgravity and darkness conditions may have significant influence on the chemopreventive role of Brassica sprouts against thyroid cancer cells in vitro, especially in the case of broccoli and kohlrabi sprouts.<\/jats:p>","DOI":"10.3390\/ijms262311287","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:02:07Z","timestamp":1763974927000},"page":"11287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Searching for Innovative Functional Foods: Correlation Between Chemopreventive Potential and Bioactive Compounds Accumulation in Brassica Sprouts Grown Under Altered Gravity Conditions"],"prefix":"10.3390","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9338-3677","authenticated-orcid":false,"given":"Marta","family":"Markiewicz","sequence":"first","affiliation":[{"name":"Doctoral School of Medical and Health Sciences, Jagiellonian University Medical College, \u0141azarza 16, 31-530 Cracow, Poland"},{"name":"Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-8646","authenticated-orcid":false,"given":"Agnieszka","family":"Galanty","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5101-036X","authenticated-orcid":false,"given":"Pawe\u0142","family":"Zagrodzki","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7030-3555","authenticated-orcid":false,"given":"Agata","family":"Ko\u0142odziejczyk","sequence":"additional","affiliation":[{"name":"Faculty of Space Technologies, AGH University of Technology, 36 Czarnowiejska St., 30-054 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4821-4492","authenticated-orcid":false,"given":"Pawe\u0142","family":"Pa\u015bko","sequence":"additional","affiliation":[{"name":"Department of Food Chemistry and Nutrition, Jagiellonian University Medical College, Medyczna 9, 30-688 Krak\u00f3w, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4779","DOI":"10.2174\/1381612825666190116124233","article-title":"Cruciferous Vegetables as Antioxidative, Chemopreventive and Antineoplasic Functional Foods: Preclinical and Clinical Evidences of Sulforaphane Against Prostate Cancers","volume":"24","author":"Ferreira","year":"2018","journal-title":"Curr. 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